检索结果-南通市图书馆

Identification of ABA-responsive genes in rice shoots via cDNA macroar- ray

Cell Research 2003年第1期13卷 59-68页

作者： FANG LIN, SHOU LING XU, WEI MIN NI, ZHAO QING CHU, ZHI HONG XU, HONG WEI XUENational Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science (SIBS), Chinese Academy of Sciences and Partner Group of Max-Planck-Institute of Molecular Plant Physiology (MPI-MP) on "Plant Molecular Physiology and Signal Transduc-tion", 300 Fenglin Road, 200032 Shanghai, China 1National Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS) Chinese Academy of Sciences and Partner Group of Max-Planck-Institute of Molecular Plant Physiology (MPI-MP) on Plant Molecular Physiology and Signal Transduction 300 Fenglin Road 200032 Shanghai China 1National Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS) Chinese Academy of Sciences and Partner Group of Max-Planck-Institute of Molecular Plant Physiology (MPI-MP) on Plant Molecular Physiology and Signal Transduction 300 Fenglin Road 200032 Shanghai China 1National Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS) Chinese Academy of Sciences and Partner Group of Max-Planck-Institute of Molecular Plant Physiology (MPI-MP) on Plant Molecular Physiology and Signal Transduction 300 Fenglin Road 200032 Shanghai China 1National Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS) Chinese Academy of Sciences and Partner Group of Max-Planck-Institute of Molecular Plant Physiology (MPI-MP) on Plant Molecular Physiology and Signal Transduction 300 Fenglin Road 200032 Shanghai China 1National Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS) Chinese Academy of Sciences and Partner Group of Max-Planck-Institute of Molecular Plant Physiology (MPI-MP) on Plant Molecular Physiology and Signal Transduction 300 Fenglin Road 200032 Shanghai China 1National Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS) Chinese Academy of Sciences and Partner Group of Max-Planck-Institute of Molecular Plant Physiology (MPI-MP) on Plant Molecular Physiology and Signal Transduct

Phytohormone abscisic acid (ABA) was critical for many plant growth and developmental processes including seed maturation, germination and response to environmental factors. With the purpose to detect the possible ABA related signal transduction pathways, we tried to isolate ABA-regulated genes through cDNA macroarray technology using ABA-treated rice seedling as materials (under treatment for 2, 4, 8 and 12 h). Of 6144 cDNA clones tested, 37 differential clones showing induction or suppression for at least one time, were isolated. Of them 30 and 7 were up- or down-regulated respectively. Sequence analyses revealed that the putative encoded proteins were involved in different possible processes, including transcription, metabolism and resistance, photosynthesis, signal transduction, and seed maturation. 6 cDNA clones were found to encode proteins with unknown functions. Regulation by ABA of 7 selected clones relating to signal transduction or metabolism was confirmed by reverse transcription PCR. In add

关键词： oryza saliva abscisic acid (ABA) cDNA macroarray responsive genes signal transduc- tion

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Expression pattern and functional analysis of a MADS-box gene M79 from rice

引用

Science China(Life Sciences) 2001年第2期44卷 161-169页

作者：瞿礼嘉张毅李春升刘美华王金玲黄葆英李志英王石平顾红雅陈章良 1. National Laboratory of Protein Engineering and Plant Genetic Engineering Peking University 100871 Beijing China 2. National Laboratory of Genetic Improvement for Crops Central China Agriculture University 430070 Wuhan China 3. The Gateway De Montfort University LE1 9BH Leicester UK

Using a degenerated primer and a T-primer, a MADS-box gene, M79,was amplified by RT-PCR from rice fluorescence at meiosis stage and then cloned. Sequence analysis shows that M79 shares 98.2% homology with OsMADS7 at DNA level while only 92% at the amino acid level. The transcript of M79 possesses five different polyadenylation sites. Only a single copy of M79 gene has been found in rice genome, which is located on chromosome 8. M79 is expressed specifically in flower organs, from pre-meiosis stage through pollen maturation. Ectopic expression of M79 in T0 and T1 transgenic rice results in early-flowering, implying that M79 is involved in controlling the flowering time. In the same time, M79 may be involved in controlling the branching process to make more flower buds.

关键词： oryza saliva MADS-box flowering time control.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A comparison of nitrate transport in four different rice (oryza sativa L.) cultivars

引用

Science China Life Sciences 2005年第S2期48卷 897-911页

作者： Anthony J.Miller Crop Performance and Improvement Division Rothamsted Research Harpenden U.K. Hertfordshire AL5 2JQ

As rice can use both nitrate (NO3-) and ammonium (NH4+), we have tested the hypothesis that the shift in the pattern of cultivars grown in Jiangsu Province reflects the ability of the plants to exploit NO3- as a nitrogen (N) source. Four rice cultivars were grown in solution culture for comparison of their growth on NO3- and NH4+ nitrogen sources. All four types of rice, Xian You 63 (XY63), Yang Dao 6 (YD), Nong Keng 57 (NK) and Si You 917 (SY917), grew well and produced similar amounts of shoot biomass with 1 mmol/L NH4+ as the only N source. However, the roots of NK were significantly smaller in comparison with the other cultivars. When supplied with 1 mmol/L NO3- YD produced the greatest biomass; while NK achieved the lowest growth among the four cultivars. Electrophysiological measurements on root rhizodermal cells showed that the NO3- elicited changes in membrane potential (△Em) of these four rice cultivars were significantly different when exposed to low external NO3- (<1 mmol/L); while they were very similar at high external NO3- (10 mmol/L). The root cell membrane potentials of YD and XY63 were more responsive to low external NO3- than those of NK and SY917. The△Em values for YD and XY63 rhizodermal cells were almost the same at both 0.1 mmol/L and 1 mmol/L NO3-; while for the NK and SY917 the values became larger as the external NO3- increased. For YD cultivar,△Em was measured over a range of NO3- concentrations and a Michaelis-Menten fit to the data gave a Km value of 0.17 mmol/L. Net NO3- uptake depletion kinetics were also compared and for some cultivars (YD and XY63) a single-phase uptake system with first order kinetics best fitted the data; while other cultivars (ND and SY917) showed a better fit to two uptake systems. These uptake systems had two affinity ranges: one had a similar Km in all the cultivars (0.2 mmol/L); the other much higher affinity system (0.03 mmol/L) was only present in NK and SY917. The expression pattern of twelve different NO3-

关键词： oryza saliva rice cultivars nitrate uptake nitrate transporters.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Plant regeneration from cultured protoplasts of a glutinous rice

引用

Cell Research 1990年第1期1卷 17-21页

作者： Wang Guangyuan ,Hsia Chenau(Xia Zhenao), Chi Jinfen and Gao XiaoyanShanghai Institute of Plant Physiology, Academia Sinica Shanghai Institute of Plant Physiology Academia Sinica

Young embryos of rice （oryza sativa L. subsp, japonicavar. Guo-xiang No. 1） were cultured on MS agar medium（2,4-D 2 mg/l）. Calli were formed and subcultured on N6 agar medium （2,4-D 2 mg/l ）. After selection, the small, grainy and pale yellowish cell clusters with dense cytoplasm were used in protoplast preparation. Isolated protoplasts were cultured in N6 medium （2,4-D 1 mg/l, 6-BA 0.2 mg/l）1 with agarose block culture method. The protoplasts grew, divided and formed calli. After inducing differentiation, the regenerated mature plants were obtained.

关键词： protoplast culture oryza saliva glutinous va-riety plant regeneration.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Microtubule Structure and Male Sterility in a Gene-Cytoplasmic Male Sterile Line of Rice, Zhen Shan 97A

引用

Acta Botanica Sinica 2003年第2期45卷 183-192页

作者：叶秀麟杨子德徐是雄梁承邺香港大学植物系加拿大卡加里大学生物系中国科学院华南植物研究所广州510650

Histological changes that occur during microsporogenesis are documented in a gene-cytoplasmic male sterile rice ( oryza saliva L.) line, Zhen Shan 97A, its maintainer line, Zhen Shan 97B, and the restorer line, Ce64 of a Mine hybrid rice production system. In the restorer line, Ce64, the developing microsporocytes have dense cytoplasm and a distinct set of circumferential microtubules around the nucleus. Successive cytokinesis results in the formation of tetrads. The microtubules within the cells of tetrads and microspores radiate from the surface of the nucleus towards the outer edge of the cytoplasm. Subsequent pollen development is normal. During the course of microspore formation tubulin speckles can be found in the cytoplasm. The general pattern of development and microtubule organization in the maintainer lined Zhen Shan 97B, is similar to Ce64, except that a few more tubulin speckles appear during microspore formation. In the case of the mate sterile line, Zhen Shan 97A, a number of abnormalities can be discerned during early microsporogenesis. These include vacuoles forming within the developing microsporocyte and faintly stained microtubules with no defined distribution pattern. Prominent tubulin speckles are common within the cytoplasm. For those microsporocytes that undergo meiosis, no defined organizational patterns of microtubules can be found within the tetrad. All microspores abort soon after. Abnormalities and defects in microtubule organization observed in Zhen Shan 97A showed that complex interactions between the cytoplasm and the nucleus began at very early stage of microsporocyte development.

关键词： oryza saliva cytoplasmic male sterility microtubules microsporocyte vacuole tubulin speckles

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Embryological Studies on In Vitro Pollination in Rice

引用

Acta Botanica Sinica 2001年第9期43卷 905-909页

作者：王文和赵洁杨弘远武汉大学植物发育生物学教育部重点实验室

In. vitro pollination and its embryological studies were carried out in two japonica cultivars of rice (oryza sativa L.), 'Chunjiang 05' and '95046'. N-6 basic medium supplemented with different exogenous hormones was used for ovary culture after in vitro pollination. The main results were as follows: (1) both cultivars were induced to set kernels after in vitro pollination. The frequency of seedset was 52.1%, including 28.4% normal embryo development, 2.2% abnormal embryo development and 21.5% callus formation. (2) The processes of embryo and endosperm development after in vitro pollination were basically as normal as those in vivo, except there was some retardation in the first division of zygotes and primary endosperm nuclei as well as in their subsequent development. However, both kernels and plantlets could be produced finally. (3) A few abnormal embryos were observed, for instance, proembryos with elongated suspensor and vacuolated proembryos. (4) Two types of calli in the cultured ovaries appeared, namely, the compact callus and the fragile callus, which were able to differentiate into adventitious buds and roots.

关键词： oryza saliva in vitro pollination embryology

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Programmed cell death during microgenesis in a Honglian CMS line of rice is correlated with oxidative stress in mitochondria

Programmed cell death during microgenesis in a Honglian CMS ...

引用

2007中国科协年会专题论坛“红莲型杂交水稻学术专题研讨会”

作者： Shaoqing Li~A Cuixiang Wan~A Jin Kong~A Zaijun Zhang~A Yangsheng Li~A Yingguo Zhu~(A,B) A Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University,Wuhan, Hubei 430072, China.

The key role of mitochondria in the integration of apoptosis and oxidative stress in mammals has been documented. In plants, mitochondria are implicated in cytoplasmic male sterility (CMS), programmed cell death (PCD), and oxidative stress. However, to date there has been no evidence presented of the interplay among CMS, PCD and oxidative stress. In this study, we demonstrate that the pollen abortion of Honglian CMS line of rice (oryza sativa L.) displays a PCD phenotype. A premature loss of microspores was accompanied by several biochemical markers of apoptosis. Analysis of mitochondria revealed that during the PCD process there was disruption of the inner mitochondrial membrane potential in microspores. This disruption was correlated with excess production of reactive oxygen species and down-regulation of the activity of superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase in mitochondria. The excess accumulation of ROS spanned four stages from pollen mother cell to early-uninucleate stage, and was followed by the occurrence of PCD at meiosis. Our data suggest that the microspores suffered from severe oxidative stress during pollen development. It is likely the chronic oxidative stress triggered the tissue-specific PCD and consequently resulted in the abortion of microspores.

关键词： cytoplasmic male sterility mitochondria oryza saliva oxidative stress programmed cell death.

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：