Microtubule Structure and Male Sterility in a Gene-Cytoplasmic Male Sterile Line of Rice, Zhen Shan 97A

作     者：叶秀麟 杨子德 徐是雄 梁承邺 YE Xiu-lin;Edward Yeung;XU Shi-Xiong;LIANG Cheng-Ye

作者机构：香港大学植物系 加拿大卡加里大学生物系 中国科学院华南植物研究所广州510650 

出 版 物：《Acta Botanica Sinica》 (Acta Botanica Sinica（植物学报：英文版）)

年 卷 期：2003年第45卷第2期

页      面：183-192页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0903[农学-农业资源与环境] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 

基　　金：香港大学研究及会议资助委员会和卓越学科领域基金 国家自然科学基金 (3 0 170 0 61) 加拿大自然科学和工程研究委员会基金~~ 

主　　题：Oryza saliva cytoplasmic male sterility microtubules microsporocyte vacuole tubulin speckles 

摘      要：Histological changes that occur during microsporogenesis are documented in a gene-cytoplasmic male sterile rice ( Oryza saliva L.) line, Zhen Shan 97A, its maintainer line, Zhen Shan 97B, and the restorer line, Ce64 of a Mine hybrid rice production system. In the restorer line, Ce64, the developing microsporocytes have dense cytoplasm and a distinct set of circumferential microtubules around the nucleus. Successive cytokinesis results in the formation of tetrads. The microtubules within the cells of tetrads and microspores radiate from the surface of the nucleus towards the outer edge of the cytoplasm. Subsequent pollen development is normal. During the course of microspore formation tubulin speckles can be found in the cytoplasm. The general pattern of development and microtubule organization in the maintainer lined Zhen Shan 97B, is similar to Ce64, except that a few more tubulin speckles appear during microspore formation. In the case of the mate sterile line, Zhen Shan 97A, a number of abnormalities can be discerned during early microsporogenesis. These include vacuoles forming within the developing microsporocyte and faintly stained microtubules with no defined distribution pattern. Prominent tubulin speckles are common within the cytoplasm. For those microsporocytes that undergo meiosis, no defined organizational patterns of microtubules can be found within the tetrad. All microspores abort soon after. Abnormalities and defects in microtubule organization observed in Zhen Shan 97A showed that complex interactions between the cytoplasm and the nucleus began at very early stage of microsporocyte development.