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optical biosensing of monkeypox virus using novel recombinant silica-binding proteins for site-directed antibody immobilization

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Journal of Pharmaceutical Analysis 2024年第10期14卷 1496-1504页

作者： Xixi Song Ying Tao Sumin Bian Mohamad Sawan CenBRAIN Neurotech School of EngineeringWestlake UniversityHangzhou310030China

The efficient immobilization of capture antibodies is crucial for timely pathogen detection during global pandemic ***,we proposed a silica-binding protein featuring core functional domains(cSP).It comprises a peptide with a silica-binding tag designed to adhere to silica surfaces and tandem protein G fragments(2C2)for effective antibody *** innovation facilitates precise site-directed immobilization of antibodies onto silica *** applied cSP to silica-coated optical fibers,creating a fiber-optic biolayer interferometer(FO-BLI)biosensor capable of monitoring the monkeypox virus(MPXV)protein A29L in spiked clinical samples to rapidly detect the *** cSP-based FO-BLI biosensor for MPXV demonstrated a limit of detection(LOD)of 0.62 ng/mL in buffer,comparable to the 0.52 ng/mL LOD achieved using a conventional streptavidin(SA)-based FO-BLI ***,it achieved LODs of 0.77 ng/mL in spiked serum and 0.80 ng/mL in spiked saliva,exhibiting no cross-reactivity with other viral *** MPXV detection process was completed within 14 *** further proposed a cSP-based multi-virus biosensor strategy capable of detecting various pandemic strains,such as MPXV,the latest coronavirus disease(COVID)variants,and influenza A protein,to extend its *** proposed cSP-modified FO-BLI biosensor has a high potential for rapidly and accurately detecting MPXV antigens,making valuable contributions to epidemiological studies.

关键词： Site-directed immobilization Silica-binding proteins optical biosensing Monkeypox virus Spiked clinical samples Multi-virus biosensor

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Tunable excitonic emission of monolayer WS2 for the optical detection of DNA nucleobases

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Nano Research 2018年第3期11卷 1744-1754页

作者： Shun Feng Chunxiao Cong Namphung Peimyoo Yu Chen Jingzhi Shang Chenji Zou Bingchen Cao Lishu Wu Jing Zhang Mustafa Eginligil Xingzhi Wang Qihua Xiong Arundithi Ananthanarayanan Peng Chen Baile Zhang Ting Yu Division of Physics and Applied Physics School of Physical and Mathematical Sciences Nanyang Technological University Singapore 637371 Singapore School of Information Science and Technology Fudan University Shanghai 200433 China Key Laboratory of Flexible Uectronics (KLOFE) & Institute of Advanced Materials (IAM) Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM) Nanjing Tech University (NanjingTech) 30 South Puzhu Road Nanjing 211816 China Division of Bioengineering School of Chemical and Biomedical Engineering Nanyang Technological University Singapore 637457Singapore

Two-dimensional transition metal dichalcogenides （2D TMDs） possess a tunable excitonic light emission that is sensitive to external conditions such as electric field, strain, and chemical doping. In this work, we reveal the interactions between DNA nucleobases, i.e., adenine （A）, guanine （G）, cytosine （C）, and thymine （T） and monolayer WS2 by investigating the changes in the photoluminescence （PL） emissions of the monolayer WS2 after coating with nucleobase solutions. We found that adenine and guanine exert a clear effect on the PL profile of the monolayer WS2 and cause different PL evolution trends. In contrast, cytosine and thymine have little effect on the PL behavior. To obtain information on the interactions between the DNA bases and WS2, a series of measurements were conducted on adenine-coated WS2 monolayers, as a demonstration. The p-type doping of the WS2 monolayers on the introduction of adenine is clearly shown by both the evolution of the PL spectra and the electrical transport response. Our findings open the door for the development of label-free optical sensing approaches in which the detection signals arise from the tunable excitonic emission of the TMD itself rather than the fluorescence signals of label molecules. This dopant-selective optical response to the DNA nucleobases fills the gaps in previously reported optical biosensing methods and indicates a potential new strategy for DNA sequencing.

关键词： tungsten disulfide photoluminescence optical biosensing chemical doping

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