Optical biosensing of monkeypox virus using novel recombinant silica-binding proteins for site-directed antibody immobilization

作     者：Xixi Song Ying Tao Sumin Bian Mohamad Sawan Xixi Song;Ying Tao;Sumin Bian;Mohamad Sawan

作者机构：CenBRAIN NeurotechSchool of EngineeringWestlake UniversityHangzhou310030China 

出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)

年 卷 期：2024年第14卷第10期

页      面：1496-1504页

核心收录：

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100401[医学-流行病与卫生统计学] 10[医学] 

基　　金：This research was supported by Westlake University,China(Startup funds) the Research Center for Industries of the Future of Westlake University,China(Grant No.:WU2022C040) the National Natural Science Foundation of China(Grant No.:82104122) 

主　　题：Site-directed immobilization Silica-binding proteins Optical biosensing Monkeypox virus Spiked clinical samples Multi-virus biosensor 

摘      要：The efficient immobilization of capture antibodies is crucial for timely pathogen detection during global pandemic ***,we proposed a silica-binding protein featuring core functional domains(cSP).It comprises a peptide with a silica-binding tag designed to adhere to silica surfaces and tandem protein G fragments(2C2)for effective antibody *** innovation facilitates precise site-directed immobilization of antibodies onto silica *** applied cSP to silica-coated optical fibers,creating a fiber-optic biolayer interferometer(FO-BLI)biosensor capable of monitoring the monkeypox virus(MPXV)protein A29L in spiked clinical samples to rapidly detect the *** cSP-based FO-BLI biosensor for MPXV demonstrated a limit of detection(LOD)of 0.62 ng/mL in buffer,comparable to the 0.52 ng/mL LOD achieved using a conventional streptavidin(SA)-based FO-BLI ***,it achieved LODs of 0.77 ng/mL in spiked serum and 0.80 ng/mL in spiked saliva,exhibiting no cross-reactivity with other viral *** MPXV detection process was completed within 14 *** further proposed a cSP-based multi-virus biosensor strategy capable of detecting various pandemic strains,such as MPXV,the latest coronavirus disease(COVID)variants,and influenza A protein,to extend its *** proposed cSP-modified FO-BLI biosensor has a high potential for rapidly and accurately detecting MPXV antigens,making valuable contributions to epidemiological studies.