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Aircraft noise exposure affects rat behavior,plasma norepinephrine levels,and cell morphology of the temporal lobe

中国药理学会第十一次全国学术会议

作者： SUN Peng~1,SUN Shi-guang~2,WEI Sheng~1 (1.Key Laboratory of the Classical Theory of Traditional Chinese Medicine of Ministry of Education,Shandong University of Traditional Chinese Medicine,Jinan 250355,China 2.Brain Behavioral Analysis Laboratory,Department of Pharmacy,Second Affiliated Hospita of Shandong University of Traditional Chinese Medicine/Shandong Provincial Hospital of Integrated Medicine,Jinan 250001,China)

OBJECTIVE To explore the correlation of open field test(OFT) and light/dark box(LDB),as two animal models of state anxiety in Kunming *** The behavior of adult,male,Kunming mice in OFT and LDB was recorded by sequence, for five minutes,with a one-week inter-trial *** following parameters were evaluated:percentage of time exploring in the OFT central area(OFTtime%);percentage of squares crossing in the OFT central area(OFTcross%);total number of squares crossing in OFT(OFTross);total number of rears in the OFT(OFTear);number of fecal boli in OFT(OFTB);percentage of time exploring in the LDB light area(LDB Ltime%);percentage of squares crossing in the LDB light area(LDB Lcross%);and percentage of rears in the LDB light area(LDBrear%);transitions between two areas in LDB(LDBransition);total number of squares crossing in LDB(LDBross);total number of rears in LDB(LDBear);number of fecal boli in LDB(LDB B).Subsequently,factor analysis,cluster analysis and correlation analysis were calculated for these ***①Factor analysis and cluster analysis of variances from either OFT or LDB revealed a three-dimension structure:OFT/LDB-anxiety factor (loaded by OFTtime%,OFTcross%or LDBtime%,LDBcross%,LDBrear%),OFT/LDB-activity factor(loaded by OFTross,OFTear or LDBransition,LDBross,LDBear) and OFT/LDB-emotionality factor(loaded by OFTB or LDBFB).While all the variances from both OFT and LDB in combination were analyzed,it could be seen as five components:DB-anxiety factor(loaded by LDBtime%,LDBcross%and LDBrear%),FT-anxiety factor(loaded by OFTtime%and OFTCcross%),DB-activity factor(loaded by LDBransition,LDBross and LDBear),motionality factor(loaded by OFTB and LDBB) and FT-activity factor(loaded by OFTross and OFTear).②Correlation analysis displayed good correlations between OFT-emotionality factor and LDB-emotionality factor(Pearson =0.383,P<0.001),as same as LDB-anxiety factor and DB-anxiety factor(Pearson = 0.989,P<0.001),OFT-anxiety factor and FT-a

关键词： open field test light/dark box state anxiety animal model factor analysis cluster analysis construct validit

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第12期12卷 969-975页

作者： Guo-qing DI Bing ZHOU Zheng-guang LI Qi-li LIN Institute for Environmental Pollution Control Technology Zhejiang University Hangzhou 310058 China

In order to investigate the physiological effects of airport noise exposure on organisms,in this study,we exposed Sprague-Dawley rats in soundproof chambers to previously recorded aircraft-related noise for 65 *** comparison,we also used unexposed control *** was arranged according to aircraft flight schedules and was adjusted to its weighted equivalent continuous perceived noise levels(LWECPN) of 75 and 80 dB for the two experimental *** examined rat behaviors through an open field test and measured the concentrations of plasma norepinephrine(NE) by high performance liquid chromatography-fluorimetric detection(HPLC-FLD).We also examined the morphologies of neurons and synapses in the temporal lobe by transmission electron microscopy(TEM).Our results showed that rats exposed to airport noise of 80 dB had significantly lower line crossing number(P<0.05) and significantly longer center area duration(P<0.05) than control *** 29 d of airport noise exposure,the concentration of plasma NE of exposed rats was significantly higher than that of the control group(P<0.05).We also determined that the neuron and synapsis of the temporal lobe of rats showed signs of damage after aircraft noise of 80 dB exposure for 65 *** conclusion,exposing rats to long-term aircraft noise affects their behaviors,plasma NE levels,and cell morphology of the temporal lobe.

关键词： Aircraft noise open field test Norepinephrine Neuron Synapse

Use of infrared thermography to detect reactions to stressful events:does animal personality matter?

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Integrative Zoology 2024年第2期19卷 224-239页

作者： Maria Vittoria MAZZAMUTO Marina MORANDINI William LAMPMAN Lucas Armand WAUTERS Damiano PREATONI John Lad KOPROWSKI Adriano MARTINOLI School of Natural Resources and Environment College of Agriculture and Life ScienceUniversity of ArizonaArizonaUSA Unitàdi Analisi e Gestione delle Risorse Ambientali Dipartimento di Scienze Teoriche e ApplicateGuido Tosi Research GroupUniversitàdegli Studi dell’InsubriaVareseItaly Haub School of Environment and Natural Resources University of WyomingWyomingUSA

The study of the relationship between animal stress and personality for free-ranging animals is limited and provides contrasting *** perception of stressors by an individual may vary due to its personality,and certain personality traits may help individuals to better cope with *** non-invasive infrared thermography(IRT),we investigated the link between physiological and behavioral components expressed during an acute stress event by free-ranging Fremont’s squirrels(Tamiasciurus fremonti).We expected that,during the acute stress event of being approached by the researcher,individuals that showed a fast pace-of-life syndrome(bolder,more active,and less social/more aggressive)based on an arena test would exhibit stronger sympathetic-adrenal-medullary system reactivity showing a more intense stress-induced hyperthermia(high core body temperature and low peripheral temperature)than individuals with a slow pace of life(shy,less active,and more social).We successfully employed IRT technology to images of Fremont’s squirrels with identification of the individuals’body parts(eye,nose,ear,hind foot).However,we found no support for our ***’body surface temperatures told us more about a squirrel’s external environment and less about the thermal state of the body in that environment following a stressful *** studies need to assess how to make IRT effective and efficient in the field and improve its performance in studying the relationships between physiology and personality in wildlife.

关键词： non-invasive method open field test physiology stress-induced hyperthermia Tamiasciurus fremonti

Genome-wide association study identifies quantitative trait loci affecting cattle temperament

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Zoological Research 2022年第1期43卷 14-25页

作者： Jia-Fei Shen Qiu-Ming Chen Feng-Wei Zhang Quratulain Hanif Bi-Zhi Huang Ning-Bo Chen Kai-Xing Qu Jing-Xi Zhan Hong Chen Yu Jiang Chu-Zhao Lei Key Laboratory of Animal Genetics Breeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityYanglingShaanxi 712100China College of Animal Science Xinjiang Agricultural UniversityUrumqiXinjiang 830052China National Institute for Biotechnology and Genetic Engineering Pakistan Institute of Engineering and Applied SciencesFaisalabad 577Pakistan Yunnan Academy of Grassland and Animal Science KunmingYunnan 650212China Academy of Science and Technology Chuxiong Normal UniversityChuxiongYunnan 675000China

Cattle temperament is an interesting trait due to its correlation with production efficiency,labor safety,and animal *** date,however,its genetic basis is not clearly ***,we performed a genome-wide association study for a series of temperament traits in cattle,assessed with via open field and novel object tests,using autosomal single nucleotide polymorphisms(SNPs)derived from the whole-genome *** identified 37 and 29 genome-wide significant loci in the open field and novel object tests,*** set analysis revealed the most significant pathway was the neuroactive ligand-receptor interaction pathway,which may be essential for emotional control in *** of the expression levels of 18 tissue-specific genes based on transcriptomic data showed enrichment in the brain,with some candidate genes involved in psychiatric and neurodegenerative diseases in *** on principal component analysis,the first principal component explained the largest variance in the open field and novel object test data,and the most significant loci were assigned to SORCS3 and SESTD1,*** findings should help facilitate cattle breeding for sound temperament by pyramiding favorable alleles to further improve cattle production.

关键词： Cattle temperament open field test Novel object test SORCS3 SESTD1

Orbitofrontal cortex action of 5-hydroxytryptamine and its receptor in an acute forced swimming stress-induced depression model

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Neural Regeneration Research 2009年第7期4卷 530-535页

作者： Huipeng Li Fengli An Shucheng An College of Life Science Shaanxi Normal University Xi'an 710062 Shaanxi Province China

BACKGROUND： The orbitofrontal cortex （OFC） is a brain region closely associated with emotion. 5-hydroxytryptamine （5-HT） has been shown to be involved in human depression. OBJECTIVE： To investigate OFC actions and mechanisms of 5-HT and 5-HT1A receptor （5-HT1AR） in stress-induced depression.DESIGN, TIME AND SETTING： A randomized, controlled, animal experiment was performed at Laboratory of Neurobiology, College of Life Science, Shaanxi Normal University between May 2006 and March 2008. MATERIALS： 5-HT, p-chlorophenylalanine （PCPA, an inhibitor to tryptophan hydroxylase） and spiperone （5-HT1AR antagonist） were provided by Sigma, USA; rabbit anti-rat 5-HT1AR antibody was provided by Tianjin Haoyang Biological Manufacture. METHODS： A total of 40 male Sprague Dawley rats, aged 3 months, were randomly divided into five groups： control, model, 5-HT, spiperone ＋ 5-HT, and PCPA, with 8 rats in each group. Except for control group, rats in the other four groups were used to establish depression models by forced swimming for 15 minutes. At 30 minutes before forced swimming test, 0.5 pL of 5-HT （12.5 pg/pL）, PCPA （20 pg/pL）, spiperone （1.3 pg/pL）＋ 5-HT （12.5 pg/pL, 10 minutes later）, and saline were respectively injected into the OFC of 5-HT, PCPA, spiperone ＋ 5-HT, and model groups, respectively. The control group received a saline microinjection into the OFC.MAIN OUTCOME MEASURES： Forced swimming and open field tests were employed to measure animal behaviors, and immunohistochemistry was used to analyze 5-HT1AR expression in the OFC, cingulate cortex, and piriform cortex. RESULTS：（1） Compared with the model group, 5-HT microinjection into the OFC prominently reduced immobility time in the forced swimming test and rearing in open field test （P 〈 0.05）; locomotion and grooming in open field test were increased, although there was no significance （P 〉 0.05）. Furthermore, following PCPA microinjection into the OFC （PCPA ＋ forced swimming stress）, immobility time in f

关键词： depression orbitofrontal cortex 5-hydroxytryptamine forced swimming test open field test

Neuronal protein-tyrosine phosphatase 1B hinders sensory-motor functional recovery and causes affective disorders in two different focal ischemic stroke models

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Neural Regeneration Research 2021年第1期16卷 129-136页

作者： Shelly A.Cruz Zhaohong Qin Konrad M.Ricke Alexandre F.R.Stewart Hsiao-Huei Chen Ottawa Hospital Research Institute Neuroscience ProgramUniversity of OttawaOttawaONCanada Brain and Mind Institute University of OttawaOttawaONCanada Department of Biochemistry Microbiology and ImmunologyUniversity of OttawaOttawaONCanada Cellular and Molecular Medicine University of OttawaOttawaONCanada Department of Medicine University of OttawaOttawaONCanada Centre for Infection Immunity and InflammationUniversity of OttawaOttawaONCanada University of Ottawa Heart Institute OttawaONCanada

Ischemic brain injury causes neuronal death and *** activates protein-tyrosine phosphatase 1B(PTP1B).Here,we tested the significance of PTP1B activation in glutamatergic projection neurons on functional recovery in two models of stroke:by photothrombosis,focal ischemic lesions were induced in the sensorimotor cortex(SM stroke)or in the peri-prefrontal cortex(peri-PFC stroke).Elevated PTP1B expression was detected at 4 days and up to 6 weeks after *** ablation of PTP1B in neurons of neuronal knockout(NKO)mice had no effect on the volume or resorption of ischemic lesions,markedly different effects on functional recovery were *** stroke caused severe sensory and motor deficits(adhesive removal test)in wild type and NKO mice at 4 days,but NKO mice showed drastically improved sensory and motor functional recovery at 8 *** addition,peri-PFC stroke caused anxiety-like behaviors(elevated plus maze and open field tests),and depression-like behaviors(forced swimming and tail suspension tests)in wild type mice 9 and 28 days after stroke,respectively,with minimal effect on sensory and motor ***-PFC stroke-induced affective disorders were associated with fewer active(FosB+)neurons in the PFC and nucleus accumbens but more FosB+neurons in the basolateral amygdala,compared to sham-operated *** contrast,mice with neuronal ablation of PTP1B were protected from anxiety-like and depression-like behaviors and showed no change in FosB+neurons after peri-PFC *** together,our study identifies neuronal PTP1B as a key component that hinders sensory and motor functional recovery and also contributes to the development of anxiety-like and depression-like behaviors after ***,PTP1B may represent a novel therapeutic target to improve stroke *** procedures for animal use were approved by the Animal Care and Use Committee of the University of Ottawa Animal Care and Veterinary Service(protocol 1806)on July 27,2018.

关键词： adhesive removal test anxiety depression elevated plus maze forced swimming test Iba1 interleukin-1β microglia open field test tail suspension test tumor necrosis factor-α

Influence of nitric oxide synthase inhibitor on gerbil behavior after hyperbaric oxygen-induced convulsion

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Neural Regeneration Research 2008年第8期3卷 903-906页

作者： Jianguang Zhou Changyun Liu Yiqun Fang Yingqi Zhou Erli XU Jingchang Liu The 411 Hospital of Chinese PLA Shanghai 200081 China Naval Medical Research Institute of Chinese PLA Shanghai 200433 China

BACKGROUND：Studies have reported that nitric oxide synthase （NOS） inhibitor can prolong the latency of hyperbaric oxygen-induced convulsion （HBOC）. However, there are very few reports addressing the influence of NOS inhibitor on mental behavior. OBJECTIVE： To investigate behavioral changes after HBOC in gerbils, as well as the influence of NOS inhibitor. DESIGN, TIME AND SETTING： Randomized experiments were performed in the Laboratory of Hyperbaric Pressure and Diving Physiology, Naval Medical Research Institute of Chinese PLA （Shanghai, China） from March 2005 to June 2007. MATERIALS： Forty male gerbils were randomly divided into five groups： HBOC, saline control, NOS inhibitor, pressure control, and normal control. Each group contained eight animals. METHODS： In the HBOC group, once depression induction ended, animals were removed from the chamber five minutes after the first appearance of generalized convulsion induced by 0.5 MPa hyperbaric oxygen. Ten minutes before entering the chamber, saline control and NOS inhibitor animals were intraperitoneally injected with 1 mL saline and 20 mg/kg NG-nitro-L-arginine, respectively. The pressure control group was only exposed to 0.5 MPa. The remaining procedures in these three groups were identical to the HBOC group. The normal control group received no intervention. MAIN OUTCOME MEASURES： open field test scores in gerbils prior to HBOC, as well as immediately, 24 hours, and 72 hours after decompression ended. RESULTS： HBOC was not detected in either the normal control or the pressure control group, and there were no significant differences in open field test scores prior to and after HBOC （P 〉 0.05）. HBOC occurred in the HBOC, saline control, and NOS inhibitor groups, with significant differences in open field test scores after decompression ended compared to normal control and pressure control groups （P 〈 0.05–0.01）. Compared to the HBOC and saline control groups, the NOS inhibitor group exhibited a significantly lower s

关键词： behavior nitric oxide synthase oxygen-induced convulsion open field test

Overexpression of brain-derived neurotrophic factor in the hippocampus protects against post-stroke depression

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Neural Regeneration Research 2015年第9期10卷 1427-1432页

作者： Hao-hao Chen Ning Zhang Wei-yun Li Ma-rong Fang Hui Zhang Yuan-shu Fang Ming-xing Ding Xiao-yan Fu Medical Molecular Biology Laboratory School of Medicine Jinhua Polytechnic Institute of Neuroscience School of Medicine Zhejiang University Center of Experimental Animals Jinhua Food and Drug Administration

Post-stroke depression is associated with reduced expression of brain-derived neurotrophic factor （BDNF）. In this study, we evaluated whether BDNF overexpression affects depression-like behavior in a rat model of post-stroke depression. The middle cerebral artery was occluded to produce a model of focal cerebral ischemia. These rats were then subjected to isolation-housing combined with chronic unpredictable mild stress to generate a model of post-stroke depression. A BDNF gene lentiviral vector was injected into the hippocampus. At 7 days after injection, western blot assay and real-time quantitative PCR revealed that BDNF expression in the hippo- campus was increased in depressive rats injected with BDNF lentivirus compared with depressive rats injected with control vector. Furthermore, sucrose solution consumption was higher, and horizontal and vertical movement scores were increased in the open field test in these rats as well. These findings suggest that BDNF overexpression in the hippocampus of post-stroke depressive rats alleviates depression-like behaviors.

关键词： nerve regeneration brain injury brain-derived neurotrophic factor lentivirus post-strokedepression depression-like behavior hippocampus cerebral ischemia sucrose solution consumption open field test chronic unpredictable mild stress western blot assay neural regeneration

Effects of extremely low frequency magnetic field on anxiety level and spatial memory of adult rats

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Chinese Medical Journal 2011年第20期124卷 3362-3366页

作者： HE Li-hua SHI Hong-mei LIUTong-tong XU Ying-chun YE Kang-ping WANG Sheng Department of Occupational and Environmental Health Peking University Health Science Center 38 Xueyuan Road Haidian District Beijing 100191 China Beijing Chongwen District Center for Disease Control and Prevention 16 Xixiaoshi Street Chongwen District Beijing 100050 China Chinese Center for Health Education Building 12 Anhuaxili 1District Andingmenwai Avenue Beijing 100011 China

Background As the widespread use of electric devices in modern life, human are exposed to extremely low frequency magnetic fields （ELF MF） much more frequently than ever. Over the past decades, a substantial number of epidemiological and experimental studies have demonstrated that ELF MF （50 Hz） exposure is associated with increased risk of various health effects. The present study examined the effects of chronic exposure to ELF MF on anxiety level and spatial memory of adult rats. Methods The 50-Hz ELF MF was used during the whole experimental procedures and the value of magnetic field （MF） was set to 2 mT. Adult rats were divided randomly to control, MF 1 hour and MF 4 hours group. Anxiety-related behaviors were examined in the open field test and the elevated plus maze; changes in spatial learning and memory were determined in Morris water maze aRer 4 weeks of daily exposure. Results Rats in MF 4 hours group had increased anxiety-like behaviors with unaltered locomotor activity. In the Morris water maze test, rats had reduced latency to find the hidden platform and improved long-term memory of former location of platform without changes in short-term memory and locomotor activity. Conclusion Chronic ELF MF exposure has anxiogenic effect on rats, and the promoting effects on spatial learning and long-term retention of spatial memory.

关键词： extremely low frequency magnetic field anxiety spatial memory Morris water maze test open field test