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Identification of genes responsive to apoptosis in HL-60 cells

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Acta Pharmacologica Sinica 2004年第3期25卷 65-72页

作者： Wei JIN2,Le-feng QU3,Ping MIN2,Shan CHEN2,Hong LI4,He LU2,4,Yong-tai HOU2,5 2Shanghai Instituteof Materia Medica,Chinese Academyof Sciences,Shanghai 201203 3Departmentof Vascular Surgery & Institute of Vascular Surgery of PLA,Changhai Hospital,Shanghai 200433 4 Le p?le franco-chinois du science du vivant et génomique,Ruijin Hospital,Shanghai 200025,China Shanghai Institute of Materia Medica Chinese Academy of Sciences Shanghai 201203 China

AIM: To identify genes responsive to apoptosis in HL-60 cells treated by homoharringtonine. METHODS: cDNA microarray technology was used to detect gene expression and the result of microarrays for genes (TIEG and VDUP1) was confirmed by Northern analysis. RESULTS: Seventy-five individual mRNAs whose mass changed significantly were identified. Among these genes (25 were up-regulated and 50 were down-regulated), most are known related to oncogenes and tumor suppressor. Some genes were involved in apoptosis signaling pathways. CONCLUSION: TGFβ and TNF apoptosis signaling pathways were initiated during apoptosis in HL-60 cells. TIEG and VDUP1 play important roles in mediating apoptosis.

关键词： HL-60 cells homoharringtonine apoptosis oligonucleotide array sequence analysis gene expression

Effect of acupuncture at Renying(ST 9) on gene expression profile of hypothalamus in spontaneously hypertensive rats

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Journal of Traditional Chinese Medicine 2018年第2期38卷 227-241页

作者： Guo Yan Lu Juan Liang Jingrong Zhao Ruili Xu Jing Zhang Wei Park Kibeum Zhu Shipeng Chen Huan Ma Liangxiao Department of Acupuncture and Moxibustion First Hospital Affiliated to Tianjin College of Traditional Chinese Medicine College of Acupuncture-Moxibustion and Tuina Beijing University of Chinese Medicine College of Acupuncture-Moxibustion and Tuina Second School of Clinical Medical of Nanjing University of Traditional Chinese Medicine Department of Acupuncture and Moxibustion Jiangsu Province Hospital

OBJECTIVE: To investigate changes in gene expression profiles in the hypothalamus related to the effects of acupuncture at the Renying(ST 9) acupoint in spontaneously hypertensive(SH) ***: We randomly divided 18 SH rats into Renying(ST 9) group and model control group, 9 body weight-matched Wistar-Kyoto rats were used as blank controls. Acupuncture was performed manually for 20-min daily over 28 d in the Renying(ST 9) group. Rat Gene 2.0 array technology was used for the determination of gene expression profiles and the screened key genes were verified by real-time quantitative polymerase chain reaction(RT-PCR) ***: The different groups exhibited differential gene expression: compared with the blank control group, 48 genes were up-regulated and 91 genes were down-regulated in the model group;compared with the model group, 79 genes were up-regulated and 80 genes were down-regulated in Renying(ST 9) group. The RT-PCR results of the key genes including Chi3 l1, Ephx2, Klk1, 5-HT1 A and Cbs were consistent with that of gene chip ***: Acupuncture at Renying(ST 9)could significantly lower the blood pressure of SH rats and affect their hypothalamic gene expression profile. Genes associated with the contraction of vascular smooth muscle and the regulation of inflammation, neurotransmitters may be involved in acupuncture's antihypertensive mechanism.

关键词： Essential hypertension Point ST 9(Re-nying) oligonucleotide array sequence analysis Hypothalamus

Genotype of ethanol metabolizing enzyme genes by oligonucleotide microarray in alcoholic liver disease in Chinese people

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Chinese Medical Journal 2002年第7期115卷 1085-1087,156+155页

作者：虞朝辉厉有名陈卫星乐敏浙江大学医学院第一附属医院杭州310003

OBJECTIVE: To explore the relationship between genetic polymorphisms of the ethanol metabolizing enzymes and the occurrence of alcoholic liver disease (ALD). METHODS: Sixty-five healthy male controls and 165 alcoholisms (including 122 ALD patients and 43 male alcohol abusers without liver complications defined as alcohol-dependent) were analyzed by polymerase chain reaction and hybridized with oligonucleotide microarray to detect the polymorphisms of the ethanol metabolizing enzymes genes. RESULTS: The frequencies of alcohol dehydrogenase gene 2 * 1 ( ADH2 * 1 ) allele were shown as 37.69%, 46.51% and 59.02% in control, alcohol-dependent and ALD groups respectively; while those of ADH2 * 2 allele were shown as 62.31 %, 53.49% and 40.98% respectively. No ADH2 * 3 was detected in any of the subjects. The frequency of ADH2 * 1 was significantly higher in alcoholisms (ALD group and alcohol-dependent group) than in healthy controls ( P

关键词： oligonucleotide array sequence analysis Alcohol Dehydrogenase Aldehyde Oxidoreductases Genotype Humans Isoenzymes Liver Diseases, Alcoholic Male Polymorphism, Genetic

Polymorphisms of GSTM1 and CYP1A1 genes and their genetic susceptibility to prostate cancer in Chinese men

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Chinese Medical Journal 2008年第4期121卷 305-308页

作者： LIMing GUAN Tong-yu LI Yao NA Yan-qun Department of Urology Peking University School of Oncology Beijing Cancer Hospital & Institute Beijing 100036 China Department of Urology First Hospital Peking University Beijing 100034 China State Key Laboratory of Genetic Engineering Institute of Genetics School of Life Science Fudan University Shanghai 200433 China

Background Variation in prostate cancer incidence between different racial groups has been well documented, for which genetic polymorphisms are hypothesized to be an explanation. We evaluated the association between polymorphisms in the cytochrome P-450 CYP1A1 （CYP1A1） and glutathione S-transferase M1 （GSTM1） genes and genetic susceptibility to prostate cancer in Chinese men.Methods TWO hundred and eight prostate cancer patients and 230 age matched controls were enrolled in this study. All DNA samples from peripheral blood lymphocytes were genotyped for common genetic polymorphisms of the CYP1A1 and GSTM1 genes using the oligonucleotide microarray （DNA chip） technique and the polymorphism results confirmed by sequencing. The different polymorphisms in prostate cancer patients were also analyzed according to age at diagnosis, prostate specific antigen level, cancer stage and grade （Gleason score）.Results The prevalence of the GSTM1 （0/0） genotype was significantly higher in prostate cancer patients （58.2%） than in controls （41.7%, P〈0.05）. Further analysis demonstrated that the prostate cancer patients with a GSTM1 （0/0） genotype were younger than those with the GSTM1 （＋/＋） genotype （P=-0.024）. No significant differences in the frequency distributions of CYP1A1 polymorphisms were observed between prostate cancer patients and controls.Conclusion GSTM1 （0/0）-gene polymorphism may be linked to prostate cancer risk and early age of Onset in Chinese.

关键词： cytochrome P-450 CYP1A1 glutathione S-transferase M1 oligonucleotide array sequence analysis prostatic neoplasms

analysis of differentially expressed genes in fetal skin of scarless and scar-forming periods of gestational rats

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Chinese Journal of Traumatology 2006年第2期9卷 94-99页

作者：陈伟付小兵葛世丽孙同柱盛志勇 Key Research Laboratory of Wound Repair Burn Institute 304th Clinical DepartmentGeneral Hospital of PLABeijing 100037China Institute of Radiation Medicine Beijing 100850 China

Objective ：To study the differences of gene expression between earlier gestational skin and later gestational skin of rats with the aids of single primer amplification （SPA） and high-density oligonucleotide DNA array to understand the molecular mechanism of scarless healing. Methods： Total RNAs were isolated from fetal rat skin of the scarless（E15） and scar-forming （ E18 ） periods of gestation （term = 21.5 days）. The RNAs from earlier gestational skin （ EGS ） and later gestational skin （ LGS ） were both reversely transcribed to cDNAs, then labeled with the incorporation of fluorescent dCTP for preparing the hybridization probes by SPA method. The mixed probes were then hybridized to the oligonucleotide DNA arrays which contained 5 705 probes representing 5 705 rat genes. After highly stringent washing, these DNA arrays were scanned for fluorescent signals to display the differentially expressed genes between the 2 groups of skin. Results. Among 5 705 rat genes, there were 53 genes （0.93%） with differentially expressed levels between EGS and LGS groups, 27 genes, including fibroblast growth factor 2 （ FGF2 ） and follistatin were up-regulated （0.47%） and 26 genes were down-regulated （0.46%） in fetal skin during scarless period versus scar-forming period. Higher expressions of FGF2 and follistatin in EGS than those in LGS were also revealed by RT-PCR method. Conclusions： High-density oligonucleotide DNA array provided a powerful tool for investigating differential gene expression in earlier and later gestational fetal skins. This technology validates that the mechanism of fetal scarless healing is very complicate and the change of many gene expressions is associated with fetal scarless healing.

关键词： Skin Gene expression oligonucleotide array sequence analysis Gestion Scarless healing

Study on Effects of Acupuncture at the Heart Meridian on Gene Expression Pattern of Hypothalamus in Rats with Acute Myocardial Ischemia

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Journal of Acupuncture and Tuina Science 2008年第5期6卷 292-294页

作者：吴子建汪克明周逸平蔡荣林龙迪和何璐刘靖 Research Institute of Acupuncture and Moxibustion Anhui College of TCMHefeiChina 230038

Objective： To discovery the central mechanism of acupuncture Heart Meridian precondition myocardial ischemia in gene expression pattern, the authors applied gene chip tech to filter variably expressed genes in hypothalamus. Methods： Rats were seperated into normal, model, acupuncture Heart Meridian group and acup Lung Meridian group randomly. Acute myocardial ischemia rat model was made with ligation left anterior descending branch of the coronary artery. After model succeed, select hypothalamus seperately and mixed the same group together of 3 rats. Then applied Rats U230A genechip refered by Affymetrix Co. to compare the variations between these groups. Results： To compare with normal group, differential expression genes and expression sequence tags （ESTs） in model group were 73 with signal log ratio ≥ 1 and 92 with signal log ratio ≤-1 , mainly included ion channel, calcium/ calmodulin-dependent protein kinase Ⅱ inhibitor, antigen and so on. Similarly, compared with model group, differential expression genes and expression sequence tags （ESTs） were 190 with signal log ratio ≥ 1 and 34 with signal log ratio ≤-1 in acupuncture Heart Meridian group, mainly included 5-hydroxytryptamine receptor, cellular metabolism, fatty, immuno reaction, G-protein coupled receptors, ion transport, signal transductions and so on, while in acupucnturc Lung Meridian, the number is 57 and 26 correspondly. Conclusion： There were exactly variations in hypothalamus mechanism that relate to acupuncture Heart and Lung Meridians.

关键词： Acupuncture Heart Meridian Lung Meridian oligonucleotide array sequence analysis Hypothalamus Acute myocardial ischemia Rats

Statistics and data analysis for microarrays using R and Bioconductor

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