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Biocatalytic synthesis of (R)-(-)-mandelic acid from racemic mandelonitrile by a newly isolated nitrilase-producer Alcaligenes sp. ECU0401

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Chinese Chemical Letters 2007年第6期18卷 677-680页

作者： Yu Cai He Jian He Xu Yi Xu Li Ming Ouyang Jiang Pan Laboratory of Biocatalysis and Bioprocessing State Key Laboratory of Bioreactor Engineering East China University of Science and Technology Shanghai 200237 China

By using acetonitrile as the sole nitrogen source, a microbial strain with high nitrilase activity, named as Alcaligenes sp. ECU0401, was newly isolated from soil, which could enantioselectively transform racemic mand... 详细信息

关键词： Mandelonitrile nitrilase (R)-()-Mandelic acid Screening Alcaligenes sp. ECU0401

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Increased β-Cyanoalanine nitrilase Activity Improves Cyanide Tolerance and Assimilation in Arabidopsis

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Molecular Plant 2014年第1期7卷 231-243页

作者： Brendan O'Leary GaU M. Preston Lee J. Sweetlove Department of Plant Sciences University of Oxford Oxford UK

Plants naturally produce cyanide （CN） which is maintained at low levels in their cells by a process of rapid assimilation. However, high concentrations of environmental CN associated with activities such as industrial pollution are toxic to plants. There is thus an interest in increasing the CN detoxification capacity of plants as a potential route to phytoremediation. Here, Arabidopsis seedlings overexpressing the Pseudomonas fluorescens β-cyanoalanine nitrilase pinA were compared with wild-type and a β-cyanoalanine nitrilase knockout line （△Atnit4） for growth in the presence of exogenous CN. After incubation with CN, ＋PfpinA seedlings had increased root length, increased fresh weight, and decreased leaf bleaching compared with wild-type, indicating increased CN tolerance. The increased tolerance was achieved without an increase in β-cyanoalanine synthase activity, the other enzyme in the cyanide assimilation pathway, suggesting that nitrilase activity is the limiting factor for cyanide detoxification. Labeling experiments with [^13C] KCN demonstrated that the altered CN tolerance could be explained by differences in flux from CN to Asn caused by altered β-cyanoalanine nitrilase activity. Metabolite profiling after CN treatment provided new insight into downstream metabolism, revealing onward metabolism of Asn by the photorespiratory nitrogen cycle and accumulation of aromatic amino acids.

关键词： cyanide β-cyanoalanine cyanide detoxification asparagine metabolism nitrilase

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Evidence That the Auxin Signaling Pathway Interacts with Plant Stress Response

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Acta Botanica Sinica 2002年第5期44卷 532-536页

作者：包方李家洋中国科学院遗传与发育生物学研究所北京100101

Auxin influences a variety of developmental and physiological processes. Early reports, suggested that auxin might affect plant stress response. We have identified a number of auxin responsive genes in Arabidopsis thaliana (L.) Heynh. by using cDNA an-ay and found that stress responsive genes, such as,Arabidopsis homolog of MEK kinase 1 (ATMEKK1), ReL/SpoT homolog 3 ( At-RSH3), Catalase 1 ( Cat1) and Ferritin 1 (Fer1), were down-regulated by auxin, indicating that auxin regulates ale expression of stress responsive genes. We also demonstrated that nitrilase genes, nitrilase I ( NIT]) and nitrilase 2 (NIT2) involving in indole-3-acetic acid (IAA) biosynthesis, were induced by salinity stress, suggesting that the level of IAA might increase in response to salinity stress. To dissect the signal pathway involved in the interaction, two auxin insensitive mutants, auxin resistant 2 (axr2) and auxin resistant 1-3 (axrl-3) were used. Stress responsive genes were induced by salt stress in wild type and axr2, but not in axr1-3. The result suggests that die interaction between auxin and stress responses may be linked in the ubiquitin pathway.

关键词： auxin stress-responsive genes auxin insensitive mutants nitrilase Arabidopsis thaliana

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