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Virologica Sinica 2021年第4期36卷 818-822页

作者： Xiaolong Lu Xiaoquan Wang Tiansong Zhan Yifan Sun Xin Wang Naiqing Xu Tianxing Liao Yu Chen Min Gu Shunlin Hu Xiaowen Liu Xiufan Liu Animal Infectious Disease Laboratory College of Veterinary MedicineYangzhou UniversityYangzhou 225000China Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis Yangzhou UniversityYangzhou 225000China Jiangsu Key Laboratory of Zoonosis Yangzhou UniversityYangzhou 225000China

Dear Editor,newcastle disease(ND),caused by virulent newcastle disease virus(NDV),is a highly contagious and economically devastating viral disease of birds(Habib et al.2018).NDV,also termed as avian paramyxovirus type 1(APMV-1),belongs to the genus Orthoavulavirus in the family Paramyxoviridae according to the International Committee on Taxonomy of Viruses(ICTV)(Amarasinghe et al.2019).According to the latest phylogenetic classification system.

关键词： newcastle ClassⅠ Poultry

Application of newcastle disease virus in the treatment of colorectal cancer

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World Journal of Clinical Cases 2019年第16期7卷 2143-2154页

作者： Hui Song Li-Ping Zhong Jian He Yong Huang Yong-Xiang Zhao National Center for International Research of Bio-targeting Theranostics Guangxi Key Laboratory of Biotargeting Theranostics Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy Guangxi Medical University

Colorectal cancer (CRC) is one of the main reasons of tumor-related deaths *** present,the main treatment is surgery,but the results are unsatisfactory,and the prognosis is *** majority of patients die due to liver or lung metastasis or *** recent years,great progress has been made in the field of tumor gene therapy,providing a new treatment for combating *** oncolytic viruses selectively replicate almost exclusively in the cytoplasm of tumor cells and do not require integration into the host genome,they are safer,more effective and more attractive as oncolytic *** disease virus (NDV) is a natural RNA oncolytic *** NDV selectively infects tumor cells,the immune response induced by NDV’s envelope protein and intracellular factors can effectively kill the tumor without affecting normal *** genetic techniques make NDV a vector for gene *** the virus by inserting various exogenous genes or using NDV in combination with immunotherapy can also improve the anti-CRC capacity of NDV,and good results have been achieved in animal models and clinical treatment *** article reviews the molecular biological characteristics and oncolytic mechanism of NDV and discusses in vitro and in vivo experiments on NDV anti-CRC capacity and clinical *** conclusion,NDV is an excellent candidate for cancer treatment,but more preclinical studies and clinical trials are needed to ensure its safety and efficacy.

关键词： newcastle disease virus Exogenous gene Armed virus Oncolytic therapy Colorectal cancer

Production and application of recombinant haemagglutinin neuraminidase of newcastle disease virus

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Asian Pacific Journal of Tropical Medicine 2010年第8期3卷 629-632页

作者： Selvan Nallaiyan Ranjit Singh Amirtha Jacob Abbadorai Sukumar Sundaramoorthy Jeyakumar Nelson Suryanarayana Veluvarthy Venkata Sanyasi Centre for Biotechnology Muthayammal College of Arts & ScienceRasipuram -637 408Tamil NaduIndia Department of Advanced Zoology Sri Paramakalyani CollegeAlwarkuruchi - 627412Tamil NaduIndia Centralized Instrumentation Laboratory Madras Veterinary CollegeChennai - 600 007Tamil NaduIndia Unit of Microbiology Faculty of MedicineAIMST UniversityMalaysia Molecular Virology Laboratory Indian Veterinary Research Institute CampusHebbalBangalore-560024KamatakaIndia

Objective:To discuss the possibility of expressing the haemagglutinin-neuraminidase（HN） protein in prokaryotic system such as Escherichia coli（***） cells by cloning the full length HN ***:The full length HN gene of newcastle Disease Virus（NDV） of size 1 734 bp was preciously isolated by *** sequence was assessed and submitted to Nucleic Acid Databank（NCBI） and the gene ID was EU215390.1 after cloning and *** the assessed HN gene was subcloned into pET 32 a+ expression vector for production the HN protein in ***, BL21（DE3） PLYSS cells following standard *** crude lysate protein from the induced positive clone was size assessed by sodium dodecyl sulfale-polyacrylamide gel electrophoresis （SDS-PAGE） and their haemagglutination（HA） property against chicken RBC was assessed by standard micro HA ***:The molecular size of the full HN gene of NDV as assessed by cloning and digesting the positive clone to release the insert was 1.7 *** expressed protein in both crude and pure form was assessed to be 63 kDa and 81 kDa,*** HA activity of the crude protein of the positive clone was 1 in ***:This finding indicates that the fusion protein retains the biological activity of native protein in the crude form and therefore could be used as a diagnostic reagent for antibody detection and for routine assessment of immune status in commercial layer forms.

关键词： newcastle disease virus Haemagglutinin Neuraminidase Surface glycoprotein Diagnostic reagent

Message from Lord Mayor of newcastle

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技术经济与管理研究 1995年第6期 13-14页

作者： JohnMcNaughtonAM

newcastle Disease Virus Isolation and Its Prevalence in Uganda Poultry Farms

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Open Journal of Veterinary Medicine 2014年第1期4卷 1-5页

作者： Keneth Iceland Kasozi Paul Ssuna Dickson Stuart Tayebwa Mohd Alyas Central Diagnostic Laboratory Department of Pharmacy Clinics and Comparative Medicine College of Veterinary Medicine Animal Resources and Biosecurity Makerere University Kampala Uganda entral Diagnostic Laboratory Department of Pharmacy Clinics and Comparative Medicine College of Veterinary Medicine Animal Resources and Biosecurity Makerere University Kampala Uganda Indian Veterinary Research Institute (IVRI) Central Avian Research Institute (CARI) Izatnagar India

The present research work was carried out to isolate and identify newcastle disease virus (NDV) by using haemagglutination inhibition (HI) test and HA-HI virus isolation, embryonated eggs (EE) and chicken embryo fibroblasts (CEF). A total of 95 clinical (blood, tracheal and cloacal swabs) and post-mortem (brain, lung, colon and spleen) samples were collected from chickens of field outbreaks of suspected newcastle disease virus (NDV). The HI and HA-HI were employed to detect NDV in tissue homogenates of all the clinical and post-mortem samples as well as laboratory samples (AF and ICF). Among the four different types of post-mortem samples, virus isolation rate was found to be low in body organs. In CEF cell culture system, the rate of virus isolation from all the aforesaid samples was found to be at 100% with the exception of serum samples;while in tracheal and cloacal swabs, it was at 90%;while in serum, it was at 10%, in all clinical cases. The isolation rate of NDV was higher in CEF culture system (66.7%) compared to that of avian embryos (33.3%). Samples were inoculated and the allantoic fluid (AF) of the dead embryos and the infected culture fluid (ICF) of the CEF were harvested at 24 to 96 hours of the post-infection, respectively, which revealed that the virulent strain of NDV is highly prevalent in the region. The prevalence of NDV was established at 1.1%, 2.1% and 4.2% using HA-HI, EE, and CEF methods. Rapid detection and identification of the virus are crucial for the effective control of the disease as conventional diagnostic methods such as virus isolation on embryonated eggs followed by serological identification in haemagglutination-inhibition test are laborious and time-consuming. The speed of the diagnosis can be considerably increased by using methods based on molecular biology, e.g. reverse transcription—polymerase chain reaction. However, the genetic variability of APMV-1 isolates should be considered carefully as the potential cause for false neg

关键词： newcastle Disease Virus Chicken Embryo Virus Isolation Poultry Farmers in Uganda Avain Viruses in Africa Animal Production Challenges

Comparative Evaluation of the Effects of Velogenic newcastle Disease Virus Infection on the Hematology of Ducks and Chickens

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Open Journal of Veterinary Medicine 2014年第6期4卷 113-121页

作者： Chekwube Paul Eze John Osita Arinze Okoye Innocent Okonkwo Ogbonna Wilfred Sunday Ezema Didacus Chukwuemeka Eze Emmanuel Chukwudi Okwor Christian Okorie-Kanu Kalu Idika Idika Department of Veterinary Parasitology and Entomology University of Nigeria Nsukka Nigeria Department of Veterinary Pathology and Microbiology University of Agriculture Umudike Nigeria Department of Veterinary Pathology and Microbiology University of Nigeria Nsukka Nigeria Microbiology Unit Department of Biological Sciences University of Agriculture Makurdi Nigeria

The hematological lesions consequent upon velogenic newcastle disease virus (NDV) infection were investigated in 6-week-old ducks and chickens. Following intramuscular inoculation, the results indicated significantly lower (p < 0.05) packed cell volume (PCV) in infected chickens (IC) on days 3 - 9 post inoculation (PI) and in infected ducks (ID) on days 3 - 15 PI. The hemoglobin concentrations were significantly lower (p < 0.05) in IC on days 3, 6 and 15 PI while in the ID, they were significantly lower (p < 0.05) on days 3, 9 and 15 PI. The total erythrocyte counts were significantly lower (p < 0.05) in IC on days 3, 9 and 15 PI and in ID, they were significantly lower (p < 0.05) on days 3 and 9 PI. The mean corpuscular values indicated macrocytic hypochromic anemia in IC and macrocytic normochromic anemia in ID. The leucogram showed leucopenia in IC and initial leucopenia followed by leucocytosis in ID. The hematological pictures of the velogenic NDV in this experiment indicate less susceptibility of ducks when compared with the chickens. The severity of this virus infection in chickens and the mild clinical signs and lesions presented by ducks showed that ducks are far less susceptible than chickens.

关键词： Velogenic newcastle Disease Experimental Infection Hematology Ducks Chickens

Comparison of the Serum Proteins and Immune Responses of Velogenic newcastle Disease Virus Infected Chickens and Ducks

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Open Journal of Veterinary Medicine 2014年第6期4卷 122-128页

作者： Chekwube Paul Eze Vincent S. O. Shoyinka John Osita Arinze Okoye Wilfred Sunday Ezema Innocent Okonkwo Ogbonna Didacus Chukwuemeka Eze Emmanuel Chukwudi Okwor Ogbu Kenneth Ikejiofor Department of Veterinary Pathology and Microbiology University of Nigeria Nsukka Nigeria Microbiology Unit Department of Biological Sciences University of Agriculture Makurdi Nigeria National Veterinary Research Institute Vom Nigeria

The effect of velogenic newcastle disease virus (vNDV) on the immune responses and serum proteins was investigated in six-week-old ducks and chickens. Results showed that weight loss was markedly significant (p < 0.05... 详细信息

关键词： Velogenic newcastle Disease Experimental Infection Immune Response Serum Proteins Ducks Chickens

Effects of the Soil-Derived Microorganism BX-1 on Chicken newcastle Disease

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Advances in Infectious Diseases 2020年第1期10卷 1-10页

作者： Kiyotaka Murakami Kazuhide Adachi Retno Damajanti Soejoedono Ekowati Handharyani Yasuhiro Tsukamoto Department of Animal Hygiene Graduate School of Environmental & Biological Sciences Kyoto Prefecture University Kyoto Japan R&D Section Taiga Inc. Tokyo Japan Faculty of Veterinary Medicine Bogor Agriculture University Bogor Indonesia

In recent years, effective microorganisms (EMs) have been administered to humans and domestic animals, and their usefulness has been recognized for promoting health and enhancing immunity. For example, the preventative effects against flu are enhanced by ingestion of Lactobacillus by humans, and symptom relief of atopic dermatitis has been reported, with EMs actually used in commercial products. In addition, EM preparations are being used in livestock to prevent infections (e.g. Salmonella and Escherichia coli infection). In poultry, avian influenza and newcastle disease are terrible and fatal infectious diseases that cause significant economic damage. Furthermore, countries designated as contaminated with these pathogens can experience major trade problems. Given the above, how to protect livestock from infections safely and at low cost without using disinfectants, antibiotics and vaccines is a major issue. In the present study, we examined whether or not newcastle disease could be suppressed by feeding chickens BX-1 as an EM feed. A field strain of newcastle virus was cloned from cloaca swabs of large numbers of dying chickens in a poultry farm in Indonesia by polymerase chain reaction (PCR) and hemaggregation assays. Chicken kidney cells and embryonated eggs were highly sensitive to this virus, and high titers of virus were able to be collected. The experimental viral inoculated to chickens showed a high mortality rate, with high pathogenicity in birds. Conventional chickens were also raised on a diet supplemented with BX-1 and directly infected with the newcastle virus. The mortality was decreased in these infected birds. Even the low dose of BX-1 had an inhibitory effect on the lethality of the infection. These results suggest that BX-1 intake through an EM diet is effective in controlling newcastle disease.

关键词： newcastle Disease Effective Microorganisms BX-1 Chicken

Antibodies to newcastle Disease Virus in Egg Yolks of Great Cormorant (<i>Phalacrocorax carbo</i>) at Qinghai Lake

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Advances in Infectious Diseases 2014年第4期4卷 194-197页

作者： Alexander Y. Alekseeev Kirill A. Sharshov Vasiliy Y. Marchenko Zhuo Li Jian Cao Fang Yang Alexander M. Shestopalov Vyacheslav A. Shkurupy Laixing Li Northwest Institute of Plateau Biology Chinese Academy of Sciences Xining China Research Center of Clinical and Experimental Medicine Siberian Branch of Russian Academia Medical Science Novosibirsk Russia Novosibirsk State University Novosibirsk Russia State Research Center of Virology and Biotechnology “Vector” Novosibirsk Russia

newcastle disease virus (NDV) is not considered as cause of serious disease in humans. But, recent data make it clear that, under particular circumstances, it is indeed possible for NDV to cause severe human respiratory disease. newcastle Disease infection has been reported in many bird species. Cormorants that inhabit at the Qinghai-Tibet Plateau are mainly represented by Great Cormorant (Phalacrocorax carbo sinensis), which is distributed mainly on the Qinghai Lake area. Cormorants are considered as one of the main NDV-reservoir. We conducted the study for the presence of antibodies to newcastle disease virus by hemagglutination inhibition test in yolks. We got 50% of seropositive yolks to newcastle disease virus. These results show that NDV circulates in the Qinghai Lake population of cormorants. We first used the technique of detection of antibodies to newcastle disease virus in the egg yolk for study the circulation of the virus in cormorants and demonstrated its effectiveness. We should carefully monitor cases of pneumonia in the population of people living around the lake and assess the causes of the disease.

关键词： newcastle Diseases Virus Antibodies Egg Yolk Great Cormorant Hemagglutination Inhibition Test

Establishment of a Real-time Fluorescent Quantitative RTPCR Method for Detecting NP Gene of Class Ⅰ newcastle Disease Virus(NDV)

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Agricultural Biotechnology 2018年第6期7卷 16-19,24页

作者： Junping CAO Xiaoquan WANG Han CHENG Xiaowen LIU Xiufan LIU College of Veterinary Medicine Yangzhou UniversityYangzhou 225009China Jiangsu Agri-animal Husbandry Vocational College Taizhou 225300China

newcastle disease( ND) is one of the most serious infectious diseases that infect the poultry *** is only one serotype of newcastle disease virus( NDV),but NDVs can be divided into two distinct classes( class Ⅰ,and class Ⅱ) according to their genetic *** develop a method for rapid quantitative detection of class Ⅰ NDV,a pair of primers and a TaqM an probe were designed and synthesized according to the conservative sequence of NP gene of class Ⅰ *** positive recombinant plasmid harboring NP gene of JS-18-05 isolate was used as a positive template to establish the standard curve.A real-time fluorescent quantitative RT-PCR method was established for rapid detection of class Ⅰ NDV with strong specificity,high sensitivity and good *** established method exhibited a good linear relationship within the concentration of 102 to 108 copies of NDV,by which 1 μl of 10 copy of NDV nucleic acid could be detected in the initial *** with conventional virus isolation methods,the established method had similar sensitivity and led to the same results in detecting33 class Ⅰ,class Ⅱ NDV *** study provided the basis for rapid quantitative detection of class Ⅰ NDVs and further clarification of their pathogenicity and pathogenic mechanism in poultry.

关键词： Class Ⅰ newcastle disease virus Nucleocapsid protein gene Fluorescent quantitative RT-PCR