检索结果-南通市图书馆

Microcurvature landscapes induce neural stem cell polarity and enhance neural differentiation

生物设计与制造(英文) 2023年第5期6卷 522-535页

作者： Ho-Yin Yuen Wai-Sze Yip Suet To Xin Zhao Department of Biomedical Engineering The Hong Kong Polytechnic UniversityHung HomKowloonHong Kong SARChina State Key Laboratory of Ultra-Precision Machining Technology Department of Industrial and Systems EngineeringThe Hong Kong Polytechnic UniversityHung HomKowloonHong Kong SARChina

Tissue curvature has long been recognized as an important anatomical parameter that affects intracellular behaviors,and there is emerging interest in applying cell-scale curvature as a designer property to drive cell fates for tissue engineering purposes.Although neural cells are known to undergo dramatic and terminal morphological changes during development and curvature-limiting behaviors have been demonstrated in neurite outgrowth studies,there are still crucial gaps in understanding neural cell behaviors,particularly in the context of a three-dimensional(3D)curvature landscape similar to an actual tissue engineering scaffold.In this study,we fabricated two substrates of microcurvature(curvature-substrates)that present a smooth and repeating landscape with focuses of either a concave or a convex pattern.Using these curvature-substrates,we studied the properties of morphological differentiation in N2a neuroblastoma cells.In contrast to other studies where two-dimensional(2D)curvature was demonstrated to limit neurite outgrowth,we found that both the concave and convex substrates acted as continuous and uniform mechanical protrusions that significantly enhanced neural polarity and differentiation with few morphological changes in the main cell body.This enhanced differentiation was manifested in various properties,including increased neurite length,increased nuclear displacement,and upregulation of various neural markers.By demonstrating how the micron-scale curvature landscape induces neuronal polarity,we provide further insights into the design of biomaterials utilizing the influence of surface curvature in neural tissue engineering.

关键词： Curvature neural differentiation Neurite outgrowth Mechanotransduction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Mechanical stretching of 3D hydrogels for neural stem cell differentiation

引用

Bio-Design and Manufacturing 2022年第4期5卷 714-728页

作者： Quanjing Mei Ho-Yin Yuen Xin Zhao Department of Biomedical Engineering The Hong Kong Polytechnic UniversityHung HomHong KongChina

While it is known that mechanical dynamics are influential in neural differentiation for critical processes like neurogenesis or neurodegeneration, studies on neural stem cell therapies usually focus on biochemical interactions rather than mechanical aspects, frequently resulting in low efficacy and unfulfilled potential. Therefore, current studies are attempting to elucidate the effect of mechanical stimulus on neural performance using conventional two-dimensional(2D) planar substrates. Yet, these2D substrates fail to capture the defining three-dimensional(3D) characteristics of the in vivo neural stem cell environment.To complete this research gap, we synthesized a series of soft and elastic 3D hydrogels to mimic the neural tissue mechanical environment for 3D cell culture, using long-chain polyethylene glycol diacrylate(PEGDA) and gelatin-methacryloyl(Gel MA).By varying the concentration of the polymer, we obtained biomimicking hydrogels with a tensile modulus as low as 10 k Pa and a compressive modulus as low as 0.8 k Pa. The in vitro results demonstrated that Gel MA-PEGDA hydrogels have the high biocompatibility required to support neural cell growth, proliferation, and differentiation, as well as neurite outgrowth. We then studied the effect of mechanical stretching on the behaviors of neural cells and observed that mechanical stretching could significantly enhance neurite extension and axon elongation. In addition, the neurites were more directionally oriented to the stretching direction. Immunocytochemistry and relative gene expression data also suggested that mechanical tension could upregulate the expression of neural differentiation protein and genes, including GFAP and βIII-Tubulin. Overall, this study shows that in addition to the specific mechanical properties of Gel MA-PEGDA that improve neural differentiation towards specific lineages, hydrogel stretching is also a potentially attractive strategy to improve the therapeutic outcomes of neural stem cell therapies.

关键词： Mechanical property Tensile stretching Hydrogels neural differentiation 3D cell culture

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

neural differentiation and synaptogenesis in retinal development

引用

neural Regeneration Research 2016年第2期11卷 312-318页

作者： Wen-juan Fan Xue Li Huan-ling Yao Jie-xin Deng Hong-liang Liu Zhan-jun Cui Qiang Wang Ping Wu Jin-bo Deng Institute of Neurobiology School of Life Science Henan University

To investigate the pattern of neural differentiation and synaptogenesis in the mouse retina, immunolabeling, Brd U assay and transmission electron microscopy were used. We show that the neuroblastic cell layer is the germinal zone for neural differentiation and retinal lamination. Ganglion cells differentiated initially at embryonic day 13（E13）, and at E18 horizontal cells appeared in the neuroblastic cell layer. neural stem cells in the outer neuroblastic cell layer differentiated into photoreceptor cells as early as postnatal day 0（P0）, and neural stem cells in the inner neuroblastic cell layer differentiated into bipolar cells at P7. Synapses in the retina were mainly located in the outer and inner plexiform layers. At P7, synaptophysin immunostaining appeared in presynaptic terminals in the outer and inner plexiform layers with button-like structures. After P14, presynaptic buttons were concentrated in outer and inner plexiform layers with strong staining. These data indicate that neural differentiation and synaptogenesis in the retina play important roles in the formation of retinal neural circuitry. Our study showed that the period before P14, especially between P0 and P14, represents a critical period during retinal development. Mouse eye opening occurs during that period, suggesting that cell differentiation and synaptic formation lead to the attainment of visual function.

关键词： nerve regeneration neural stem cells neural differentiation retinal development synaptogenesis neural regeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

neural differentiation of human Wharton＇s jelly-derived mesenchymal stem cells improves the recovery of neurological function after transplantation in ischemic stroke rats

引用

neural Regeneration Research 2017年第7期12卷 1103-1110页

作者： Lei Zhang Lin-mei Wang Wei-wei Chen Zhi Ma Xiao Han Cheng-ming Liu Xiang Cheng Wei Shi Jing-jing Guo Jian-bing Qin Xiao-qing Yang Guo-hua Jin Xin-hua Zhang Department of Anatomy Nantong University Nantong Jiangsu Province China Department of Radiation Oncology Third People's Hospital of Yancheng Yancheng Jiangsu Province China Department of Neurosurgery the-Affiliated Hosptial of Nantong University Nantong Jiangsu Province China Department of Obstetricsand Gynecology the Affiliated Hosptial of Nantong University Nantong Jiangsu Province China Co-innovation Center of Neuroregeneration Nantong University Nantong Jiangsu Province China

Human Wharton＇s jelly-derived mesenchymal stem cells（h WJ-MSCs）have excellent proliferative ability,differentiation ability,low immunogenicity,and can be easily obtained.However,there are few studies on their application in the treatment of ischemic stroke,therefore their therapeutic effect requires further verification.In this study,h WJ-MSCs were transplanted into an ischemic stroke rat model via the tail vein 48 hours after transient middle cerebral artery occlusion.After 4 weeks,neurological functions of the rats implanted with h WJ-MSCs were significantly recovered.Furthermore,many h WJ-MSCs homed to the ischemic frontal cortex whereby they differentiated into neuron-like cells at this region.These results confirm that h WJ-MSCs transplanted into the ischemic stroke rat can differentiate into neuron-like cells to improve rat neurological function and behavior.

关键词： nerve regeneration human Wharton＇s jelly-derived mesenchymal stem cells ischemic stroke cell transplantation middle cerebral arteryocclusion neural differentiation neurological function neural regeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Multiple factors to assist human-derived induced pluripotent stem cells to efficiently differentiate into midbrain dopaminergic neurons

引用

neural Regeneration Research 2024年第4期19卷 908-914页

作者： Yalan Chen Junxin Kuang Yimei Niu Hongyao Zhu Xiaoxia Chen Kwok-Fai So Anding Xu Lingling Shi Guangdong-Hong Kong-Macao Institute of CNS Regeneration Ministry of Education CNS Regeneration Collaborative Joint LaboratoryJinan University Department of Neurology and Stroke Center The First Affiliated HospitalJinan University Department of Psychiatry The First Affiliated HospitalJinan University Co-innovation Center of Neuroregeneration Nantong University

Midbrain dopaminergic neurons play an important role in the etiology of neurodevelopmental and neurodegenerative diseases.They also represent a potential source of transplanted cells for therapeutic applications.In vitro differentiation of functional midbrain dopaminergic neurons provides an accessible platform to study midbrain neuronal dysfunction and can be used to examine obstacles to dopaminergic neuronal development.Emerging evidence and impressive advances in human induced pluripotent stem cells,with tuned neural induction and differentiation protocols,makes the production of induced pluripotent stem cell-derived dopaminergic neurons feasible.Using SB431542 and dorsomorphin dual inhibitor in an induced pluripotent stem cell-derived neural induction protocol,we obtained multiple subtypes of neurons,including 20%tyrosine hydroxylase-positive dopaminergic neurons.To obtain more dopaminergic neurons,we next added sonic hedgehog(SHH)and fibroblast growth factor 8(FGF8)on day 8 of induction.This increased the proportion of dopaminergic neurons,up to 75%tyrosine hydroxylase-positive neurons,with 15%tyrosine hydroxylase and forkhead box protein A2(FOXA2)co-expressing neurons.We further optimized the induction protocol by applying the small molecule inhibitor,CHIR99021(CHIR).This helped facilitate the generation of midbrain dopaminergic neurons,and we obtained 31-74%midbrain dopaminergic neurons based on tyrosine hydroxylase and FOXA2 staining.Thus,we have established three induction protocols for dopaminergic neurons.Based on tyrosine hydroxylase and FOXA2 immunostaining analysis,the CHIR,SHH,and FGF8 combined protocol produces a much higher proportion of midbrain dopaminergic neurons,which could be an ideal resource for tackling midbrain-related diseases.

关键词： dopaminergic neurons FGF signal induced pluripotent stem cells midbrain neural differentiation SHH signal SMAD signal WNT signal

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

ARPE-19 conditioned medium promotes neural differentiation of adipose-derived mesenchymal stem cells

引用

World Journal of Stem Cells 2021年第11期13卷 1783-1796页

作者： Giuliana Mannino Martina Cristaldi Giovanni Giurdanella Rosario Emanuele Perrotta Debora Lo Furno Rosario Giuffrida Dario Rusciano Department of Biomedical and Biotechnological Sciences University of CataniaCatania 95123CTItaly Research Center SOOFT-Italia S.p.A.Catania 95123CTItaly Department of General Surgery and Medical-Surgery Specialties University of CataniaCatania 95100CTItaly

BACKGROUND Adipose-derived stem cells(ASCs)have been increasingly explored for cell-based medicine because of their numerous advantages in terms of easy availability,high proliferation rate,multipotent differentiation ability and low immunogenicity.In this respect,they have been widely investigated in the last two decades to develop therapeutic strategies for a variety of human pathologies including eye disease.In ocular diseases involving the retina,various cell types may be affected,such as Müller cells,astrocytes,photoreceptors and retinal pigment epithelium(RPE),which plays a fundamental role in the homeostasis of retinal tissue,by secreting a variety of growth factors that support retinal cells.AIM To test ASC neural differentiation using conditioned medium(CM)from an RPE cell line(ARPE-19).METHODS ASCs were isolated from adipose tissue,harvested from the subcutaneous region of healthy donors undergoing liposuction procedures.Four ASC culture conditions were investigated:ASCs cultured in basal Dulbecco's Modified Eagle Medium(DMEM);ASCs cultured in serum-free DMEM;ASCs cultured in serumfree DMEM/F12;and ASCs cultured in a CM from ARPE-19,a spontaneously arising cell line with a normal karyotype derived from a human RPE.Cell proliferation rate and viability were assessed by crystal violet and MTT assays at 1,4and 8 d of culture.At the same time points,ASC neural differentiation was evaluated by immunocytochemistry and western blot analysis for typical neuronal and glial markers:Nestin,neuronal specific enolase(NSE),protein gene product(PGP)9.5,and glial fibrillary acidic protein(GFAP).RESULTS Depending on the culture medium,ASC proliferation rate and viability showed some significant differences.Overall,less dense populations were observed in serum-free cultures,except for ASCs cultured in ARPE-19 serum-free CM.Moreover,a different cell morphology was seen in these cultures after 8 d of treatment,with more elongated cells,often showing cytoplasmic ramifications.Immunofluorescence results and western blot anal

关键词： Adipose-derived mesenchymal stem cells Retinal pigment epithelium neural markers neural differentiation Retina damage Cell-based medicine

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Histone deacetylase inhibitor promotes differentiation of embryonic stem cells into neural cells in adherent monoculture

引用

Chinese Medical Journal 2010年第6期123卷 734-738页

作者： YAO Xing ZHANG Jia-rong HUANG Hua-rong DAI Li-cheng LIU Qing-jun ZHANG Ming Department of Hepatopancreatobilary Surgery Huzhon Central Hospital Huzhou Zhejiang 313000 China College of Life Science Zhejiang University Hangzhou Zhejiang 310058 China Biosensor National Special Laboratory Department of Biomedical Engineering Zhejiang University Hangzhou Zhejiang 310027 China

Background Embryonic stem （ES） cells poss unlimited self-renewal capacity and the ability to differentiate into cell of all three germ layers in vitro. Induced differentiation of ES cells to neural lineage cells has great potential in basic study of neurogenesis and regeneration therapy of neurodegenerative diseases. Histone deacetylase （HDAC） inhibitors enhance histone acetylation so that globularly activate gene expression and may initiate multilineage differentiation. In this study,we aimed to develop a method to induce the differentiation of ES cells to neural cells combining HDAC inhibition and neural cellselection.Methods In this study, we used HDAC inhibitor sodium butyrate （NAB） to induce the differentiation of mouse embryonic stem cells to neural cells through monolayer culture. After differentiation initiation by histone deacetylase inhibitor sodium butyrate, neural cells were induced and selected with a serum free culture system. Results Homogeneous neurons without glial cells demonstrated by molecular marker expression were differentiated with the method. The resultant neurons were excitable. Conclusion The method combined differentiation induction effect of HDAC inhibitors and selective culture system to derive neural cells from ES cells, and implied the involvement of epigenetic regulation in neural differentiation.

关键词： embryonic stem cells histone deacetylase inhibitor neural differentiation adherent monoculture

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

In vitro neural differentiation of human umbilical cord blood-derived mesenchymal stem cells induced by secreted protein of olfactory ensheathing cells

In vitro neural differentiation of human umbilical cord bloo...

引用

IANR第四届和GCNN第八届年会

作者： Xiaoyu Tao Mingqiang Rong Zhenyu Zhao Yu Zeng Xiaohua Teng Da Duan Bo Liu Chen Peng Ping Chen Songping Liang Ming Lu Department of Neurosurgery Second Affiliated Hospital of Hunan Normal University(163 Hospital of PLA) Key laboratory of Protein Chemistry and Developmental Biology of Ministry of Education College of Life Sciences Hunan Normal University Changsha

Human umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) could be induced to differentiatein to neural cells under certain conditions, nevertheless, optimal protocols that could be reproducible and reliable in generating transplantable human UCB-MSCs for neurological disease in vitro are still not available. We studied for the first time the neural differentiation of human UCB-MSCs induced by secreted protein of olfactory ensheathing cells(OECs) from neonatal rat. At various time points (12h, 24h, 72h,1 week and 2 Weeks post-induced), human UCB-MSCs were morphologically observed, and processed for immunocytochemistry 1week post-induced. Whole-cell patch clamp technique was used to detect cell membrane current prior to and 2 weeks following induction. Neuron-like cells appeared 3 days following induction, and almost all human USB-MSCs appeared Neuron-like cells 1 week following induction. so as the number of immunocytochemistry cells labeled by neural markers NSE and GFAP. Our findings demonstrate that human UCB-MSCs can efficiently differentiatein to neural cells induced by secreted protein of OECs from neonatal rat and the human UCB-MSCs have the electrophysiological properties of mature neurons after 2 Weeks post-induced. The present protocol provides an alternative neurogenesis pathway for generating sufficient numbers of neural cells from human UCB-MSCs.

关键词： olfactory ensheathing cells human umbilical cord blood-derived mesenchymal stem cells neural differentiation protein

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Fibroblast growth factor 8 and retionic acid synergistically promote neural differentiation of monolayer P19 cells through inhibition of bone morphogenetic protein signaling with distinct mechanisms

Fibroblast growth factor 8 and retionic acid synergistically...

引用

中国神经科学学会第六届学术会议暨学会成立十周年庆祝大会

作者： Hisato Kondoh Ang Siew-lan Graduate School of Frontier Biosciences Osaka University 1-3 UamadaokaSuitaOsaka 565-0871Japan Division of Developmental Neurobiology National Institute for Medical Research The RidgewayMill HillLondon NW7 1AAUK

＜正＞FGF8, a member of the fibroblast growth factor （FGF） family, has been shown to play important roles in different developing systems, such as central nervous system （CNS） and limbuds. Mouse embryonic carcinoma P19 cells could be induced by retinoic acid （RA） to differentiate into neuroectodermal cell lineages, and this process is both RA and cell aggregation dependent. In this report, we showed that FGF8 mRNA and protein are transiently upregulated upon P19 cell aggregation. Overexpression of FGF8 in monolayer P19 cells could promote RA-induced P19 cell neural differentiation without aggregation. Inhibition of FGF8 expression by RNA interference （RNAi） and neutralizing of FGF8 protein by soluble FGFR3-Fc, as well as inhibition of FGF signaling by FGF receptor inhibitor, SU5402, could suppress P19 cell neural differentiation. We also showed that RA and FGF8 might induce P19 cell neural differentiation by synergistically inhibiting bone morphogenetic protein （BMP） signaling through distinct mechanisms. FGF8 inhibits Smadl nuclear translocation through Erk phosphorylation, and RA induces expression of Noggin and RA receptors, RARs, which virtually blocked Smadl-dependent transcriptional activity.

关键词： FGF retionic acid P19 cell neural differentiation

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Autophagy facilitates mouse mesenchymal stem cell neural differentiation

Autophagy facilitates mouse mesenchymal stem cell neural dif...

引用

中国神经科学学会第十届全国学术会议

作者： Bo LI Chun-Tian HUANG Jia-Cheng ZHANGU Xue-Fei HAN Yan XU Wen-Hai YAN Ying XING Stem cell research center Zhengzhou University Department of Physiology Zhengzhou University Department of Pathophysiology Zhengzhou University Department of Physiology Xinxiang medical university

Objective Autophagy is known as a process of "self-eating" and degrade long-lived proteins and unnecessary or dysfunctional organelles,recent studies shows autophagy plays a critical role in cell survival,proliferation and other physiological and pathophysiological process.However,despite its importance in cell physiology,there is little information about the role of autophagy in stem cells.We investigated the autophagy phenomenon in mouse mesenchymal stem cell and the effect in neural differentiation.Methods FACS,western blotting and immunofluorescence were performed to detect autophagy gene LC3 in mouse mesenchymal stem cell,Acridine orange and MDC staining were conducted to detect the acidic vesicle organelles.FACS and immunofluorescence were performed to detect nestin,NSE,tuj-1,GFAP after mouse mesenchymal stem cell neural differentiation with treatment of autophagy inducer rapamycine and autophagy inhibitor 3-methyladenine and chloroquine.Notchl and its downstream gene JAG1,PS1,HES1 were detected by Western-blot.Results It was found that autophagy is exist in mouse mesenchymal stem cell,treatment with rapamycine could promote mouse mesenchymal stem cell neural differentiation,and autophagy inhibitors have the opposite effect,autophagy activation downregulated Notchl expression.Conclusion Our experiment found that autophagy facilitated mouse mesenchymal stem cell neural differentiation,and Notchl signaling may involve.

关键词： mesenchymal stem cell autophagy neural differentiation Notchl

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：