Neural differentiation and synaptogenesis in retinal development

作     者：Wen-juan Fan Xue Li Huan-ling Yao Jie-xin Deng Hong-liang Liu Zhan-jun Cui Qiang Wang Ping Wu Jin-bo Deng 

作者机构：Institute of Neurobiology School of Life Science Henan University 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2016年第11卷第2期

页      面：312-318页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100212[医学-眼科学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China,No.31070952 and U1204311 the Scientific Research Foundation of Henan University of China,No.0000A40475 and 0000A40356 

主　　题：nerve regeneration neural stem cells neural differentiation retinal development synaptogenesis neural regeneration 

摘      要：To investigate the pattern of neural differentiation and synaptogenesis in the mouse retina, immunolabeling, Brd U assay and transmission electron microscopy were used. We show that the neuroblastic cell layer is the germinal zone for neural differentiation and retinal lamination. Ganglion cells differentiated initially at embryonic day 13（E13）, and at E18 horizontal cells appeared in the neuroblastic cell layer. Neural stem cells in the outer neuroblastic cell layer differentiated into photoreceptor cells as early as postnatal day 0（P0）, and neural stem cells in the inner neuroblastic cell layer differentiated into bipolar cells at P7. Synapses in the retina were mainly located in the outer and inner plexiform layers. At P7, synaptophysin immunostaining appeared in presynaptic terminals in the outer and inner plexiform layers with button-like structures. After P14, presynaptic buttons were concentrated in outer and inner plexiform layers with strong staining. These data indicate that neural differentiation and synaptogenesis in the retina play important roles in the formation of retinal neural circuitry. Our study showed that the period before P14, especially between P0 and P14, represents a critical period during retinal development. Mouse eye opening occurs during that period, suggesting that cell differentiation and synaptic formation lead to the attainment of visual function.