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A Clinical Study on the Treatment of Peripheral nerve Injury growth factor of Mecobalamin Combined with nerve

中国神经科学学会第六届学术会议暨学会成立十周年庆祝大会

作者：塔依尔·阿不都哈德尔新疆医科大学第一附属医院骨科专病医院

＜正＞目的:末梢神经损伤常常引起神经源性疼痛并且很难治愈,原因之一是这种疾病的发生机制还不完全清楚。在骨科临床上常常遇到的反射性交感神经营养不良,肩手综合征均属于神经源性疼痛。为了解明不同程度的末梢神经损伤和修复是否直... 详细信息

＜正＞目的:末梢神经损伤常常引起神经源性疼痛并且很难治愈,原因之一是这种疾病的发生机制还不完全清楚。在骨科临床上常常遇到的反射性交感神经营养不良,肩手综合征均属于神经源性疼痛。为了解明不同程度的末梢神经损伤和修复是否直接影响神经源性疼痛,单侧末梢神经损伤是否影响对侧后足皮肤和后根神经节神经生长因子（nerve growth factorNGF）浓度的改变,我设计了一种系列的脊髓神经损伤动物模型（Spinal nerve Injury Series,SNIS）,包括SNIS-I:紧密结扎左侧腰5脊髓神

关键词： neuropathic pain mirror image allodynia nerve growth factor spinal nerve injury dorsal root ganglion nerve regeneration nerve degeneration

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World Journal of Neuroscience 2016年第2期6卷 75-81页

作者： Chengkua Huang Guosheng Su Wen Wei Wenzhong Lu Yinwen Mai Shuliang Hua Yong Zhao Jili Lu The People’s Hospital in Baise Guangxi Youjiang Ethnic Southwest Hospital Affiliated to Medical School Spine Surgery Baise China The Fourth People’s Hospital Clinical Laboratory Nanning Guangxi Guangxi Medical University Affiliated to Infectious Diseases Hospital of Nanning Guangxi AIDS Clinical Treatment Center (Nanning) Nanning China

Objective: This research aims to explore the clinical curative effect for the treatment peripheral nerve injury growth factor of Mecobalamin combined with nerve. Methods: 150 cases of patients with peripheral nerve injury treated in the hospital in July were selected from 2011 to 2013. Those patients were randomly divided into three groups according to the types of injured nerve and the undergoing treatment order. 50 cases are for each group. Patients in group A were injected with 0.5 mg Mecobalamin by intravenous injection. It lasted for once a day, for 10 days. Later, 0.5 mg Mecobalamin was changed to be taken orally, three times a day, a course of treatment. And a course is 3 to 6 weeks. 50 patients in group B were injected with nerve growth factor for 30 micrograms by intramuscular injection, once a day, a course of treatment. And a course is 3 to 6 weeks. While in group C, 50 patients were treated with Mecobalamin (0.5 mg, intravenous injection, once a day) combined with nerve growth factor (30 mg, intramuscular injection, once a day). A course lasted 3 to 6 weeks. The purpose is to observe the therapeutic effect of two different drugs on patients with peripheral nerve injury. Results: The curative effect of Mecobalamin combined with nerve growth factors for the treatment of peripheral nerve injury in group C is more significant than the single drug treatment in group A and group B. Peripheral nerve injury after sensorimotor function rating evaluation of curative effect made by The British Medical Research Institute of Neurotrauma Society was applied in this research. The comparison of the results of 3 groups after treatment is showed as follows. As to the sensory recovery above level II, the curative effect in C group is generally better than in group A and group B, the approximate chi square test H = 13.6573, P = 0.0011. About motion recovery situation, there is a statistical significance in group A and group C which recovered V level X2 = 3.8431,

关键词： Mecobalamin nerve growth factor Peripheral nerve Injury

Effect of electroacupuncture at Neiguan(PC6)at different time points on myocardial ischemia reperfusion arrhythmia in rats

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Journal of Traditional Chinese Medicine 2024年第1期44卷 113-121页

作者： SUN Qianhui CHENG Kai DAI Xingye YANG Zhiwen WU Xiaoling XU Chang QIU Xinghua GAO Xiaofeng LIU Daonan YANG Qirui School of Acupuncture and Tuina Beijing University of Chinese MedicineBeijing 100029China Beijing MedEx Technology Co. Ltd.Beijing 100029China

OBJECTIVE:To observe the effects of electroacupuncture at Neiguan(PC6)at different time points on reperfusion arrhythmia(RA)after myocardial ischemia and reperfusion in rats,and to investigate the correlation of this protective effect with nerve growth factor(NGF),tyrosine kinase A(Trk A),tyrosine hydroxylase(TH),and norepinephrine(NE).METHODS:A total of 72 Sprague-Dawley male rats were randomly divided into six groups(n=12 rats/group):normal group(Norm),sham operation group(Sham),ischemia reperfusion group(I/R),pre-ischemic electroacupuncture group(EAI),pre-reperfusion electroacupuncture group(EAII),post-reperfusion electroacupuncture group(EAIII).The myocardial ischemia-reperfusion injury(MIRI)model was induced by occlusion of left anterior descending coronary artery for 20 min followed by reperfusion for 40 min in *** no intervention in the Norm group and only threading without ligation in the Sham *** pretreatment at 20 min/d for 7 d before ligation in the EAⅠgroup,20 min of electroacupuncture before reperfusion in the EAII group and 20 min of electroacupuncture after reperfusion in the EAIII *** electrocardiogram(ECG)of each group was recorded throughout the whole process,and the success of the MIRI model was determined based on the changs of J-point and T-wave in the *** arrhythmia score was used to record premature ventricular contractions,ventricular tachycardia and ventricular fibrillation during the reperfusion period to assess the reperfusion induced *** expression levels of NGF,Trk A,TH protein were measured by Western ***,the expression levels of plasma and myocardial NE levels were detected by enzyme linked immunosorbent ***:The differences between Norm group and Sham group were not statistically significant in all *** score,myocardial NGF,Trk A,TH,and NE expression were significantly higher in the I/R group compared with the Sham *** score,myocardial NGF,T

关键词： myocardial reperfusion injury reperfusion arrhythmia electroacupuncture point PC6(Neiguan) nerve growth factor protein-tyrosine kinases norepinephrine

Functional recovery after peripheral nerve injury via sustained growth factor delivery from mineral-coated microparticles

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Neural Regeneration Research 2021年第5期16卷 871-877页

作者： Daniel J.Hellenbrand Clayton L.Haldeman Jae-Sung Lee Angela G.Gableman Elena K.Dai Stephen D.Ortmann Jerrod CGotchy Kierra K.Miller Adrianna M.Doucas Nicole C.Nowak William L.Murphy Amgad S.Hanna Department of Neurological Surgery University of WisconsinMadisonWIUSA Department of Biomedical Engineering University of WisconsinMadisonWIUSA Department of Orthopedics and Rehabilitation University of Wisconsin Madison WIUSA

The gold standard for treating peripheral nerve injuries that have large nerve gaps where the nerves cannot be directly sutured back together because it creates tension on the nerve,is to incorporate an autologous nerve ***,even with the incorporation of a nerve graft,generally patients only regain a small portion of function in limbs affected by the ***,there has been some promising results using growth factors to induce more axon growth through the nerve graft,many of these previous therapies are limited in their ability to release growth factors in a sustained manner and tailor them to a desired time *** ideal drug delivery platform would deliver growth factors at therapeutic levels for enough time to grow axons the entire length of the nerve *** hypothesized that mineral coated microparticles(MCMs)would bind,stabilize and release biologically active glial cell-derived neurotrophic factor(GDNF)and nerve growth factor(NGF)in a sustained ***,the objective of this study was to test the ability of MCMs releasing growth factors at the distal end of a 10 mm sciatic nerve graft,to induce axon growth through the nerve graft and restore hind limb *** sciatic nerve grafting in Lewis rats,the hind limb function was tested weekly by measuring the angle of the ankle at toe lift-off while walking down a *** weeks after grafting,the grafts were harvested and myelinated axons were analyzed proximal to the graft,in the center of the graft,and distal to the *** physiological conditions in vitro,the MCMs delivered a burst release of NGF and GDNF for 3 days followed by a sustained release for at least 22 *** vivo,MCMs releasing NGF and GDNF at the distal end of sciatic nerve grafts resulted in significantly more myelinated axons extending distal to the graft when compared to rats that received nerve grafts without growth factor *** rats with nerve grafts incorporated with MCMs releasing NGF and G

关键词： autografts axon growth drug delivery glial cell-derived neurotrophic factor growth factors mineral coatings nerve grafting nerve growth factor

Müller cells are activated in response to retinal outer nuclear layer degeneration in rats subjected to simulated weightlessness conditions

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Neural Regeneration Research 2025年第7期20卷 2116-2128页

作者： Yuxue Mu Ning Zhang Dongyu Wei Guoqing Yang Lilingxuan Yao Xinyue Xu Yang Li Junhui Xue Zuoming Zhang Tao Chen Aerospace Clinical Medical Center School of Aerospace MedicineAir Force Medical UniversityXi’anShaanxi ProvinceChina Department of Aviation Medicine Xijing HospitalAir Force Medical UniversityXi’anShaanxi ProvinceChina Department of Emergency Medicine Wuhan No.1 HospitalWuhanHubei ProvinceChina Third Regiment School of Basic MedicineAir Force Medical UniversityXi’anShaanxi ProvinceChina Fourth Regiment School of Basic MedicineAir Force Medical UniversityXi’anShaanxi ProvinceChina

A microgravity environment has been shown to cause ocular damage and affect visual acuity,but the underlying mechanisms remain ***,we established an animal model of weightlessness via tail suspension to examine the pathological changes and molecular mechanisms of retinal damage under *** 4 weeks of tail suspension,there were no notable alterations in retinal function and morphology,while after 8 weeks of tail suspension,significant reductions in retinal function were observed,and the outer nuclear layer was thinner,with abundant apoptotic *** investigate the mechanism underlying the degenerative changes that occurred in the outer nuclear layer of the retina,proteomics was used to analyze differentially expressed proteins in rat retinas after 8 weeks of tail *** results showed that the expression levels of fibroblast growth factor 2(also known as basic fibroblast growth factor)and glial fibrillary acidic protein,which are closely related to Müller cell activation,were significantly *** addition,Müller cell regeneration and Müller cell gliosis were observed after 4 and 8 weeks,respectively,of simulated *** findings indicate that Müller cells play an important regulatory role in retinal outer nuclear layer degeneration during weightlessness.

关键词： glial fibrous acidic protein gliosis Müller cells nerve growth factor neural differentiation neurodegeneration proteomic retinal degeneration retinal outer nuclear layer simulated weightlessness

Neurotrophic factor-based pharmacological approaches in neurological disorders

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Neural Regeneration Research 2023年第6期18卷 1220-1228页

作者： Margherita Alfonsetti Michele d’Angelo Vanessa Castelli Department of Life Health and Environmental SciencesUniversity of L’AquilaL’AquilaItaly

Aging is a physiological event dependent on multiple pathways that are linked to lifespan and processes leading to cognitive *** process represents the major risk factor for aging-related diseases such as Alzheimer’s disease,Parkinson’s disease,and ischemic *** incidence of all these pathologies increases exponentially with *** on aging biology has currently focused on elucidating molecular mechanisms leading to the development of those *** deficit and neurodegeneration,common features of aging-related pathologies,are related to the alteration of the activity and levels of neurotrophic factors,such as brain-derived neurotrophic factor,nerve growth factor,and glial cell-derived neurotrophic *** this reason,treatments that modulate neurotrophin levels have acquired a great deal of interest in preventing neurodegeneration and promoting neural regeneration in several neurological *** treatments include both the direct administration of neurotrophic factors and the induced expression with viral vectors,neurotrophins’binding with biomaterials or other molecules to increase their bioavailability but also cell-based *** neurotrophins’crucial role in aging pathologies,here we discuss the involvement of several neurotrophic factors in the most common brain aging-related diseases and the most recent therapeutic approaches that provide direct and sustained neurotrophic support.

关键词： Alzheimer’s disease brain brain-derived neurotrophic factor glial cell-derived neurotrophic factor nerve growth factor neurotrophins neurturin Parkinson’s disease stroke tropomyosin receptor kinase receptors

Application of neurotrophic and proangiogenic factors as therapy after peripheral nervous system injury

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Neural Regeneration Research 2022年第6期17卷 1240-1247页

作者： Kamilla Faritovna Idrisova Alina Kazymovna Zeinalova Galina Andreevna Masgutova Alexey Andreevich Bogov Jr. Cinzia Allegrucci Valeriia Yurievna Syromiatnikova Ilnur Ildusovich Salafutdinov Ekaterna Evgenievna Garanina Dina Ivanovna Andreeva Adilet Abdullaatovich Kadyrov Albert Anatolevich Rizvanov Ruslan Faridovich Masgutov Institute of Fundamental Medicine and Biology Kazan Federal UniversityKazanRussia Republican Clinical Hospital KazanRussia Biodiscovery Institute School of Veterinary Medicine and ScienceUniversity of NottinghamNottinghamUK Department of Traumatology and Orthopedics Kazan State Medical AcademyKazanRussia

The intrinsic ability of peripheral nerves to regenerate after injury is extremely limited,especially in case of severe *** often leads to poor motor function and permanent *** approaches for the treatment of injured nerves do not provide appropriate conditions to support survival and growth of nerve *** drawback can be compensated by the use of gene therapy and cell therapy-based drugs that locally provide an increase in the key regulators of nerve growth,including neurotrophic factors and extracellular matrix *** growth factor plays its own specific angiotrophic or neurotrophic ***,growth factors are widely studied as accelerators of nerve *** noteworthy is synergy between various growth factors,that is essential for both angiogenesis and *** growth factor 2 and vascular endothelial growth factor are widely known for their proangiogenic *** the same time,fibroblast growth factor 2 and vascular endothelial growth factor stimulate neural cell growth and play an important role in neurodegenerative diseases of the peripheral nervous *** together,their neurotrophic and angiogenic properties have positive effect on the regeneration *** this review we provide an in-depth overview of the role of fibroblast growth factor 2 and vascular endothelial growth factor in the regeneration of peripheral nerves,thus demonstrating their neurotherapeutic efficacy in improving neuron survival in the peripheral nervous system.

关键词： fibroblast growth factor 2 growth factors nerve growth factor peripheral nerve injury peripheral nervous system vascular endothelial growth factor

Artificial nerve graft constructed by coculture of activated Schwann cells and human hair keratin for repair of peripheral nerve defects

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Neural Regeneration Research 2023年第5期18卷 1118-1123页

作者： Han-Jun Qin Hang Li Jun-Ze Chen Kai-Rui Zhang Xing-Qi Zhao Jian-Qiang Qin Bin Yu Jun Yang Department of Orthopedics Nanfang HospitalSouthern Medical UniversityGuangdong Provincial Key Laboratory of Bone and Cartilage Regenerative MedicineNanfang HospitalSouthern Medical UniversityGuangzhouGuangdong ProvinceChina Hebei Medical University ShijiazhuangHebei ProvinceChina Department of Orthopedics Baiyun Branch of Southern HospitalGuangzhouGuangdong ProvinceChina Department of Anatomy School of Basic Medical SciencesSouthern Medical UniversityGuangzhouGuangdong ProvinceChina Department of Orthopedics The 74th Group Military Hospital of PLAGuangzhouGuangdong ProvinceChina

Studies have shown that human hair keratin(HHK) has no antigenicity and excellent mechanical properties. Schwann cells, as unique glial cells in the peripheral nervous system, can be induced by interleukin-1β to secrete nerve growth factor, which promotes neural regeneration. Therefore, HHK with Schwann cells may be a more effective approach to repair nerve defects than HHK without Schwann cells. In this study, we established an artificial nerve graft by loading an HHK skeleton with activated Schwann cells. We found that the longitudinal HHK microfilament structure provided adhesion medium, space and direction for Schwann cells, and promoted Schwann cell growth and nerve fiber regeneration. In addition, interleukin-1β not only activates Schwann cells, but also strengthens their activity and increases the expression of nerve growth factors. Activated Schwann cells activate macrophages, and activated macrophages secrete interleukin-1β, which maintains the activity of Schwann cells. Thus, a beneficial cycle forms and promotes nerve repair. Furthermore, our studies have found that the newly constructed artificial nerve graft promotes the improvements in nerve conduction function and motor function in rats with sciatic nerve injury, and increases the expression of nerve injury repair factors fibroblast growth factor 2 and human transforming growth factor B receptor 2. These findings suggest that this artificial nerve graft effectively repairs peripheral nerve injury.

关键词： artificial nerve graft bioactive human hair keratin interleukin-1β macrophages nerve graft nerve growth factor nerve repair peripheral nervous injury Schwann cells

A new peptide,VD11,promotes structural and functional recovery after spinal cord injury

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Neural Regeneration Research 2023年第10期18卷 2260-2267页

作者： Shan-Shan Li Bai-Yu Zhang Sai-Ge Yin Zi-Qi Wei Nai-Xin Liu Yi-Lin Li Si-Yu Wang Yu-Heng Shi Jian Zhao Li-Juan Wang Yue Zhang Jun Sun Ying Wang Xin-Wang Yang Department of Anatomy and Histology&Embryology Faculty of Basic Medical ScienceKunming Medical UniversityKunmingYunnan ProvinceChina Key Laboratory of Chemistry in Ethnic Medicinal Resources&Key Laboratory of Natural Products Synthetic Biology of Ethnic Medicinal Endophytes State Ethnic Affairs Commission&Ministry of EducationSchool of Ethnic MedicineYunnan Minzu UniversityKunmingYunnan ProvinceChina Department of Urology First Affiliated Hospital of Kunming Medical UniversityKunmingYunnan ProvinceChina Yunnan Province Clinical Research Center for Chronic Kidney Disease First Affiliated Hospital of Kunming Medical UniversityKunmingYunnan ProvinceChina

The regenerative capacity of the central nervous system is very limited and few effective treatments are currently available for spinal cord *** is therefore a priority to develop new drugs that can promote structural and functional recovery after spinal cord *** studies have shown that peptides can promote substantial repair and regeneration of injured *** amphibians have a pronounced ability to regenerate the spinal cord,few studies have investigated the effect of amphibian spinal cord-derived peptides on spinal cord *** we report for the first time the successful identification and isolation of a new polypeptide,VD11(amino acid sequence:VDELWPPWLPC),from the spinal cord of an endemic Chinese amphibian(Odorrana schmackeri).In vitro experiments showed that VD11 promoted the secretion of nerve growth factor and brain-derived neurotrophic factor in BV2 cells stimulated with lipopolysaccharide,as well as the proliferation and synaptic elongation of PC12 cells subjected to *** vivo experiments showed that intravertebral injection of VD11 markedly promoted recovery of motor function in rats with spinal cord injury,alleviated pathological damage,and promoted axonal ***,RNA sequencing and western blotting showed that VD11 may affect spinal cord injury through activation of the AMPK and AKT signaling *** summary,we discovered a novel amphibian-derived peptide that promotes structural and functional recovery after spinal cord injury.

关键词： Akt signaling pathway amphibian-derived bioactive peptide AMPK signaling pathway axonal regeneration brain-derived neurotrophic factor ischemia/reperfusion injury motor function nerve growth factor neuroprotective effect spinal cord injury

单侧盆腔神经节切除术后的大鼠腹侧前列腺中的神经生长因子信号的年龄依赖性

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Asian Journal of Andrology 2013年第6期15卷 764-769,I0007页

作者： Carol A Podlasek Rudrani Ghosh Omer Onur Cakir Christopher Bond Kevin E McKenna Kevin T McVary Department of Urology Northwestern University Feinberg School of Medicine Chicago IL60611 USA Department of Physiology Northwestern University Feinberg School of Medicine Chicago IL 60611 USA Division of Urology Southern Illinois University School of Medicine Springfield IL 62794 USA

Benign prostatic hyperplasia （BPH） is a serious health concern and is an underlying cause of lower urinary tract symptoms （LUTS） in many men. In affected men, LUTS/BPH is believed to result from benign proliferation of the prostate resulting in bladder outlet obstruction. Postnatal growth of the prostate is controlled via growth factor and endocrine mechanisms. However, little attention had been given to the function of the autonomic nervous system in prostate growth and differentiation. nerve growth factor （NGF） is a prostatic mitogen that has a trophic role in autonomic sensory end organ interaction. In this study, we examine how the autonomic nervous system influences prostate growth as a function of age by quantifying NGF in the rat ventral prostate （VP） after pelvic ganglionectomy. Unilateral pelvic ganglionectomy was performed on postnatal days 30 （P30）, 60 and 120 Sprague-Dawley rats in comparison to sham controls （n=39）. Semiquantitative RT-PCR, Western blotting and immunohistochemical analysis for NGF were performed on denervated, intact （contralateral side） and sham control VP 7 days after surgery. Ngf RNA expression was significantly increased in the denervated and intact hyperplastic VP. Western blotting showed age-dependent increases in NGF protein at P60 in the contralateral intact VP. NGF was localized in the nerves, basal cells and columnar epithelium of the prostatic ducts. Denervation causes age-dependent increases in NGF in the VP, which is a potential mechanism by which the autonomic nervous system may regulate prostate growth and lead to BPH/LUTS.

关键词： denervation nerve growth factor prostate growth ventral prostate