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Analysis of Genetic Structure of natural populations of Castanopsis fargesii by RAPDs

Acta Botanica Sinica 2002年第11期44卷 1321-1326页

作者：朱其慧潘惠新诸葛强尹佟明邹惠渝黄敏仁南京林业大学林木遗传和基因工程重点实验室南京210037

Genetic diversity and population genetic structure in 188 individuals from five natural populations of Castampsis fargesii Franch. were studied by RAPD markers. Three hundred and eighty-five loci were identified with 41 oligonucleotide primers, out of which 157 loci were polymorphic and accounted for 40.78% of total genetic diversity at species level. Shannon's indices of diversity (I) and Nei's gene diversity ( h) were 0.459 7 and 0.296 at the species level, respectively. The result showed that genetic variation of C. fargesii populations mainly existed within populations. Genetic differentiation (Hsp-hpop)/Hsp estimated with Shannon's index of diversity and coefficient of gene differentiation (Gst) were 0.047 6 and 0.042 9 respectively, which were confirmed by the analysis of molecular variance (AMOVA). Therefore, it is apparent that within-population variation accounted for 94.97% and among-populations variation accounted for only 5.03% of the total genetic diversity. AMOVA also indicated that there was significant differentiation among populations as well as among individuals within a population.

关键词： Castanopsis fargesii natural populations RAPD genetic structure

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Geographical differences of leaf traits of the endangered plant Litsea coreana *** and its relationship with climate

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Journal of Forestry Research 2023年第1期34卷 125-135页

作者： Gangyi Yuan Qiqiang Guo Yaqin Zhang Qin Gui Na Xie Siqiong Luo Institute for Forest Resources and Environment of Guizhou Key Laboratory of Forest Cultivation in Plateau Mountain of Guizhou ProvinceCollege of ForestryGuizhou UniversityGuiyang 550025People’s Republic of China

Seventeen morphological and anatomical characteristics of the leaves were selected from five natural populations to explore the variation in leaf traits of Lits ea core ana *** and the effects of geographical environment on these *** analysis of variance,multiple comparisons,principal component analysis(PCA),and correlation analysis were conducted to explore the variations within and between populations and their correlation with geographical and climatic *** differences in the 17 leaf traits were observed within and among *** average,the relative contribution of within population variation to total variation was 24.8%,which was lower than among population variation(54.6%).The average differentiation coefficient of the traits was 65.8%,and the average coefficient of variation 11.8%,ranging from6.7%for main vein thickness to 21.4%for petiole *** PC A results showed that morphological characteristics were divided into two categories,and the level of variation was greater than that of leaf *** of the leaf traits were significantly correlated with geography and climate and showed a gradual variation with longitude,latitude,and *** areas with high temperatures,less rainfall,and strong seasonal rainfall,the leaves are larger,longer and *** study shows that variations in leaf traits of *** *** mainly come from variations among *** level of trait differentiation among populations is high and the level of variation within populations *** findings help further understand leaf morphological characteristics of this species and can provide a valuable reference for the protection and sustainable utilization of this natural resource.

关键词： Litsea coreana var.sinensis Climate Leaf trait variations natural populations Endangered species

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