Analysis of Genetic Structure of Natural Populations of Castanopsis fargesii by RAPDs
栲树天然群体遗传结构的RAPD分析(英文)作者机构：南京林业大学林木遗传和基因工程重点实验室南京210037
出 版 物：《Acta Botanica Sinica》 (Acta Botanica Sinica（植物学报：英文版）)
年 卷 期：2002年第44卷第11期
页 面：1321-1326页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种]
主 题：Castanopsis fargesii natural populations RAPD genetic structure
摘 要：Genetic diversity and population genetic structure in 188 individuals from five natural populations of Castampsis fargesii Franch. were studied by RAPD markers. Three hundred and eighty-five loci were identified with 41 oligonucleotide primers, out of which 157 loci were polymorphic and accounted for 40.78% of total genetic diversity at species level. Shannon s indices of diversity (I) and Nei s gene diversity ( h) were 0.459 7 and 0.296 at the species level, respectively. The result showed that genetic variation of C. fargesii populations mainly existed within populations. Genetic differentiation (Hsp-hpop)/Hsp estimated with Shannon s index of diversity and coefficient of gene differentiation (Gst) were 0.047 6 and 0.042 9 respectively, which were confirmed by the analysis of molecular variance (AMOVA). Therefore, it is apparent that within-population variation accounted for 94.97% and among-populations variation accounted for only 5.03% of the total genetic diversity. AMOVA also indicated that there was significant differentiation among populations as well as among individuals within a population.
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