检索结果-南通市图书馆

Evaluation of genetic response of mesenchymal stem cells to nanosecond pulsed electric fields by whole transcriptome sequencing

引用

World Journal of Stem Cells 2024年第3期16卷 305-323页

作者： Jian-Jing Lin Tong Ning Shi-Cheng Jia Ke-Jia Li Yong-Can Huang Qiang Liu Jian-Hao Lin Xin-Tao Zhang Department of Sports Medicine and Rehabilitation Peking University Shenzhen HospitalShenzhen 518036Guangdong ProvinceChina Institute of Medical Science The Second HospitalCheeloo College of MedicineShandong UniversityJinan 250033Shandong ProvinceChina Department of Biomedical Engineering Institute of Future TechnologyPeking UniversityBeijing 100871China Shenzhen Engineering Laboratory of Orthopaedic Regenerative Technologies Peking University Shenzhen HospitalShenzhen 518036Guangdong ProvinceChina Arthritis Clinical and Research Center Peking University People’s HospitalBeijing 100044China

BACKGROUND Mesenchymal stem cells(MSCs)modulated by various exogenous signals have been applied extensively in regenerative medicine ***,nanosecond pulsed electric fields(nsPEFs),characterized by short duration and high strength,significantly influence cell phenotypes and regulate MSCs differentiation via multiple ***,we used transcriptomics to study changes in messenger RNA(mRNA),long noncoding RNA(lncRNA),microRNA(miRNA),and circular RNA expression during nsPEFs *** To explore gene expression profiles and potential transcriptional regulatory mechanisms in MSCs pretreated with *** The impact of nsPEFs on the MSCs transcriptome was investigated through whole transcriptome *** were pretreated with 5-pulse nsPEFs(100 ns at 10 kV/cm,1 Hz),followed by total RNA *** transcript was normalized by fragments per kilobase per *** change and difference significance were applied to screen the differentially expressed genes(DEGs).Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to elucidate gene functions,complemented by quantitative polymerase chain reaction *** In total,263 DEGs were discovered,with 92 upregulated and 171 *** were predominantly enriched in epithelial cell proliferation,osteoblast differentiation,mesenchymal cell differentiation,nuclear division,and wound *** cellular components,DEGs are primarily involved in condensed chromosome,chromosomal region,actin cytoskeleton,and *** aspect of molecular functions,DEGs are mainly involved in glycosaminoglycan binding,integrin binding,nuclear steroid receptor activity,cytoskeletal motor activity,and steroid *** real-time polymerase chain reaction confirmed targeted transcript *** Our systematic investigation of the wide-ranging transcriptional pattern modulated by nsPEFs revealed the differential expression of 26

关键词： nanosecond pulsed electric fields Whole transcriptome sequencing Mesenchymal stem cells Genetic response Stem cell engineering

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

nanosecond pulsed electric fields prime mesenchymal stem cells to peptide ghrelin and enhance chondrogenesis and osteochondral defect repair in vivo

引用

Science China(Life Sciences) 2022年第5期65卷 927-939页

作者： Kejia Li Litong Fan Jianjing Lin Boon Chin Heng Zhantao Deng Qiujian Zheng Jue Zhang Yangzi Jiang Zigang Ge Department of Biomedical Engineering College of EngineeringPeking UniversityBeijing 100871China Arthritis Clinical and Research Center Peking University People’s HospitalBeijing 100044China Arthritis Institute Peking UniversityBeijing 100871China Peking University School of Stomatology Beijing 100081China Department of Orthopedics Guangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhou 510080China Institute of Biomechanics and Biomedical Engineering College of EngineeringPeking UniversityBeijing 100871China Institute for Tissue Engineering and Regenerative Medicine School of Biomedical SciencesFaculty of MedicineThe Chinese University of Hong KongHong Kong 999077China School of Biomedical Sciences Faculty of MedicineThe Chinese University of Hong KongHong Kong 999077China Department of Biomedical Engineering College of Future TechnologyPeking UniversityBeijing 100871China

Mesenchymal stem cells(MSCs) are important cell sources in cartilage tissue development and homeostasis,and multiple strategies have been developed to improve MSCs chondrogenic differentiation with an aim of promoting cartilage *** we report the effects of combining nanosecond pulsed electric fields(ns PEFs) followed by treatment with ghrelin(a hormone that stimulates release of growth hormone) to regulate chondrogenesis of *** PEFs and ghrelin were observed to separately enhance the chondrogenesis of MSCs,and the effects were significantly enhanced when the bioelectric stimulation and hormone were combined,which in turn improved osteochondral tissue repair of these cells within Sprague Dawley *** further found that ns PEFs can prime MSCs to be more receptive to subsequent stimuli of differentiation by upregulated Oct4/Nanog and activated JNK signaling *** initiated chondrogenic differentiation by activation of ERK1/2 signaling pathway,and RNA-seq results indicated 243 genes were regulated,and JAK-STAT signaling pathway was ***,the sequential order of applying these two stimuli is critical,with ns PEFs pretreatment followed by ghrelin enhanced chondrogenesis of MSCs in vitro and subsequent cartilage regeneration in vivo,but not vice *** synergistic prochondrogenic effects provide us new insights and strategies for future cell-based therapies.

关键词： mesenchymal stem cells nanosecond pulsed electric fields ghrelin chondrogenic differentiation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Recent progress in pulsed electric field ablation for liver cancer

引用

World Journal of Gastroenterology 2020年第24期26卷 3421-3431页

作者： Zhen-Guo Liu Xin-Hua Chen Zu-Jiang Yu Jun Lv Zhi-Gang Ren Department of Infectious Diseases the First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052Henan ProvinceChina Gene Hospital of Henan Province Zhengzhou 450052Henan ProvinceChina Precision Medicine Center the First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052Henan ProvinceChina Department of Hepatobiliary and Pancreatic Surgery the First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310003Zhejiang ProvinceChina Key Laboratory of Combined Multi-organ Transplantation Ministry of Public HealthHangzhou 310003Zhejiang ProvinceChina Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Hangzhou 310003China

The number of liver cancer patients is likely to continue to increase in the coming decades due to the aging of the population and changing risk *** treatments cannot meet the needs of all *** treatment methods evolved from pulsed electric field ablation are expected to lead to breakthroughs in the treatment of liver *** paper reviews the safety and efficacy of irreversible electroporation in clinical studies,the methods to detect and evaluate its ablation effect,the improvements in equipment and its antitumor effect,and animal and clinical trials on *** also summarize studies on the most novel nanosecond pulsed electric field ablation techniques in vitro and in *** research results are certain to promote the progress of pulsed electric field in the treatment of liver cancer.

关键词： Hepatocellular carcinoma pulsed electric field Irreversible electroporation Electrochemotherapy nanosecond pulsed electric fields Ablation treatment

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：