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nanobiotechnology for the Detection and Control of Waterborne Parasites

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Open Journal of Ecology 2021年第3期11卷 203-223页

作者： Wafaa M. Hikal Amra Bratovcic Rowida S. Baeshen Kirill G. Tkachenko Hussein A. H. Said-Al Ahl Department of Biology Faculty of Science University of Tabuk Tabuk KSA Water Pollution Research Department Environmental Research Division National Research Centre Giza Egypt Department of Physical Chemistry and Electrochemistry Faculty of Technology University of Tuzla Tuzla Bosnia and Herzegovina V. L. Komarov Botanical Institute of the Russian Academy of Sciences Saint Petersburg Russia Medicinal and Aromatic Plants Research Department National Research Centre Giza Egypt

Nowadays, the fast development of nanobiotechnology, has led to rapid diagnosis of important infectious diseases such as arboviruses-borne diseases, vector-borne infections and waterborne parasites diseases and others in order to reduce and avoid further dissemination of the infections within the general population. Furthermore, new nanomedicines based on the application of silver and gold nanoparticles which are less toxic, more effective, and that does not generate resistance could help to solve the problems of parasitic disease like leishmaniasis and chagas disease. It turns out that the combination of nanoparticles with antibiotics not only reduces the toxicity of both agents towards human cells but also enhances their ability to destroy bacteria by facilitating the binding of antibiotics to the microbes. Moreover, combining nanoparticles with antimicrobial peptides and essential oils with nanoparticles generates genuine synergy against microbial resistance.

关键词： Parasites Water nanobiotechnology Control

Review Article: Immobilized Molecules Using Biomaterials and nanobiotechnology

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Journal of Biomaterials and nanobiotechnology 2010年第1期1卷 61-77页

作者： Magdy M. M. Elnashar Centre of Scientific Excellence - Polymers Department - Advanced Materials & Nanotechnology Laboratory National Research Center El-Behooth St. Dokki Cairo Egypt.

Immobilized molecules using biomaterials and nanobiotechnology is a very interesting topic that touching almost all aspects of our life. It uses the sciences of biology, chemistry, physics, materials engineering and computer science to develop instruments and products that are at the cutting edge of some of today’s most promising scientific frontiers. In this review article, the author based on his experience in this arena has tried to focus on some of the supports for im-mobilization;the most important molecules to be immobilized such as DNA, cells, enzymes, metals, polysaccharides, etc and their applications in medicine, food, drug, water treatment, energy and even in aerospace. He specified a special section on what is new in the arena of supports and technologies used in enzyme immobilization and finally a recommendation by the author for future work with a special attention to up-to-date references.

关键词： Immobilized molecules Biotechnology Enzymes Biomaterials nanobiotechnology

Nanostructured biohybrid material with wide-ranging antiviral action

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Nano Research 2023年第8期16卷 11455-11463页

作者： Noelia Losada-Garcia Angela Vazquez-Calvo David Ortega-Alarcon Olga Abian Adrian Velazquez-Campoy Pilar Domingo-Calap Antonio Alcami Jose M.Palomo Instituto de Catálisis y Petroleoquímica(ICP) CSICC/Marie Curie 2.Madrid 28049Spain Centro de Biología Molecular Severo Ochoa Consejo Superior de Investigaciones Científicas(CSIC)-Universidad Autónoma de Madrid(UAM)Madrid 28049Spain Institute of Biocomputation and Physics of Complex Systems(BIFI) Joint Unit GBsC-CSIC-BIFIUniversity of ZaragozaZaragoza 50018Spain Fundación Instituto de Investigación Sanitaria de Aragón(IIS Aragón) Zaragoza 50009Spain CIBERehd Madrid 28029Spain Instituto de Biología Integrativa de Sistemas I2SyBioUniversitat de València-CSICValència E-46071Spain

Respiratory pathogens kill more people than any other infectious agent each year *** of novel,economically friendly,sustainable,and highly efficient materials against viruses is a major ***,we describe a nanostructured material composed of very small crystalline phosphate copper nanoparticles synthesized using a new biohybrid technology that employs a biological agent for its formation at room temperature in aqueous *** evaluation of different enzymes in the final preparation of the nanomaterial or even in synthetic methods was *** characterization revealed the formation of Cu species in the protein *** best biomaterial synthesized using a lipase called BioCuNPs showed excellent inhibition capacity against functional proteins of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2);for example,assent 3-chymotrypsin like protease(3CLpro)complete inhibition was achieved by using 5µg/mL,or acetone(ACE)–spike protein interaction was inhibited by more than 80%in the presence of 400µg/mL of *** these in vitro results into account,an efficacy analysis against human coronavirus 229E(HCoV-E229)coronavirus was performed.A virus reduction of 99%was obtained in 5 ***,SARS-CoV-2 virus was tested to demonstrate high efficiency,with>99%inhibition in 15 min using 500 microgram of *** determine the wide applicability of this nanohybrid against viruses,an evaluation was carried out against a non-enveloped virus such as Human Rhinovirus(HRV-14),obtaining a virus reduction of 99.9%in 5 ***,the virucidal capacity against different bacteriophages was also evaluated,obtaining an excellent inhibition effect against PhageΦX174(99.999%reduction in 5 min).

关键词： antiviral compound coronaviruses copper nanoparticles nanobiotechnology viruses

Advancing sustainable agriculture: Enhancing crop nutrition with next-generation nanotech-based fertilizers

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Nano Research 2023年第12期16卷 13205-13225页

作者： Yiwen Liao Dawei Xu Yuhong Cao Yong-Guan Zhu State Key Laboratory of Urban and Regional Ecology Research Centre for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China University of Chinese Academy of Sciences Beijing 100049China CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety National Center for Nanoscience and TechnologyBeijing 100190China

The pressing need to enhance nutrient use efficiency(NUE)in fertilizers has become increasingly urgent in light of food insecurity and climate-related *** offers promising prospects for the development of effective and environmentally friendly alternatives in the field of *** review focuses on the impact of nanotechnology on conventional fertilizers,encompassing inorganic,organic,and microbial *** emphasize the superior attributes of nano-fertilizers compared with their conventional counterparts and explore their potential and versatility in boosting crop productivity,reducing fertilizer expenses,and mitigating detrimental environmental *** conclusion,given the significant challenges posed by food insecurity and climate change,the application of nano-fertilizers demonstrates immense potential for advancing sustainable and intelligent agricultural practices.

关键词： nanobiotechnology nano-fertilizers(NFs) nutrient use efficiency(NUE) slow release sustainable agriculture

Construction of RNA nanotubes

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Nano Research 2019年第8期12卷 1952-1958页

作者： Hui Li Shaoying Wang Zhouxiang Ji Congcong Xu Lyudmila S. Shlyakhtenko Peixuan Guo Center for RNA nanobiotechnology and Nanomedicine Division of Pharmaceutics and Pharmaceutical ChemistryCollege of Pharmacy/Department of Physiology & Cell BiologyCollege of Medicine/Dorothy M. Davis Heart and Lung Research Institute and James Comprehensive Cancer CenterThe Ohio State UniversityColumbusOH43210USA UNMC Nanoimaging Core Facility Department of Pharmaceutical SciencesCollege of Pharmacy University of Nebraska Medical CenterOmahaNE68182USA Present address: University of California San FranciscoCA94158USA Present address: P&Z Biological Technology NewarkNJ07103USA

Nanotubes are miniature materials with significant potential applications in nanotechnological, medical, biological and material sciences. The quest for manufacturing methods of nano-mechanical modules is in progress. For example, the application of carbon nanotubes has been extensively investigated due to the precise width control, but the precise length control remains challenging. Here we report two approaches for the one-pot self-assembly of RNA nanotubes. For the first approach, six RNA strands were used to assemble the nanotube by forming a 11 nm long hollow channel with the inner diameter of 1.7 nm and the outside diameter of 6.3 nm. For the second approach, six RNA strands were designed to hybridize with their neighboring strands by complementary base pairing and formed a nanotube with a six-helix hollow channel similar to the nanotube assembled by the first approach. The fabricated RNA nanotubes were characterized by gel electrophoresis and atomic force microscopy (AFM), confirming the formation of nanotube-shaped RNA nanostructures. Cholesterol molecules were introduced into RNA nanotubes to facilitate their incorporation into lipid bilayer. Incubation of RNA nanotube complex with the free-standing lipid bilayer membrane under applied voltage led to discrete current signatures. Addition of peptides into the sensing chamber revealed discrete steps of current blockage. Polyarginine peptides with different lengths can be detected by current signatures, suggesting that the RNA-cholesterol complex holds the promise of achieving single molecule sensing of peptides.

关键词： RNA nanotechnology peptide sensing RNA nanotube nanobiotechnology

Translation of the long-term fundamental studies on viral DNA packaging motors into nanotechnology and nanomedicine

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Science China(Life Sciences) 2020年第8期63卷 1103-1129页

作者： Chenxi Liang Tao Weitao Lixia Zhou Peixuan Guo Center for RNA nanobiotechnology and Nanomedicine College of PharmacyCollege of MedicineDorothy M.Davis Heart and Lung Research Instituteand Comprehensive Cancer Center.The Ohio State UniversityColumbusOhio 43210USA Biology Department College of Science and MathematicsSouthwest Baptist UniversityBolivarMissouri 65613USA

Many years of fundamental studies on viral genome packaging motors have led to fruitful *** double-stranded DNA(dsDNA)viruses package their genomes into preformed protein shells via nanomotors including several elegant and meticulous coaxial *** motor is geared by the hexameric RNA *** open washer displayed as hexametric string of phi29 motor ATPase has been *** open washer linked into a filament as a queue with left-handed chirality along the dsDNA *** was found that a free 5′-and 3′-dsDNA end is not required for one gp16 dimer and four monomers to assemble into the hexametric washer on *** above studies have inspired several applications in nanotechnology and *** applications include:(i)studies on the precision motor channels have led to their application in the single pore sensing;(ii)investigations into the hand-in-hand integration of the hexametric pRNA ring have resulted in the emergence of the new field of RNA nanotechnology;and(iii)the studies on the motor stoichiometry of homologous multi-subunits that subsequently have inspired the discovery of new methods in highly potent drug *** review focuses on the structure and function of the viral DNA packaging motors and describes how fundamental studies inspired various *** these advantages,more nanotechnological and biomedical applications using bacteriophage motor components are expected.

关键词： nanopores packaging motor phi29 pRNA single pore sensing chemical detection peptide differentiation and sensing nanotechnology nanobiotechnology

The interaction of nanostructured antimicrobials with biological systems:Cellular uptake,trafficking and potential toxicity

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Food Science and Human Wellness 2020年第1期9卷 8-20页

作者： Adriano Brandelli Centro de Nanociencia e Nanotecnologia Universidade Federal do Rio Grande do SulICTA-UFRGSAv.Bento Gonccalves 950091501-970Porto AlegreBrazil

Nanomaterials have been found increasing applications in the food *** antimicrobials can be incorporated either to food matrix of food packaging or to provide extended safety and ***,the interactions and effects of nanomaterials with biological systems are still poorly *** can enter the organism by oral,dermal and inhalation routes and distributed to different tissues by the circulatory *** evidence indicate that targeting to specific tissues,cellular uptake and intracellular fate of nanoparticles are strongly influenced by size,shape and surface *** specific characteristics of nanomaterials are also determinant for their toxicity in higher *** dose,exposure time and administration route are important aspects influencing toxicity of nanoparticles as *** in vitro and in vivo evaluation studies on different types of nanostructures have providing information to support a better understanding about the interactions of nanoscale materials with biological systems.

关键词： Active packaging Antimicrobial Food safety nanobiotechnology Nanoparticle toxicity

Plant-mediated synthesis of nanoparticles:A newer and safer tool against mosquito-borne diseases?

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Asian Pacific Journal of Tropical Biomedicine 2016年第4期6卷 353-354页

作者： Giovanni Benelli Insect Behavior Group Department of AgricultureFood and EnvironmentUniversity of Pisa

Prevention and control of mosquito-borne diseases is a key challenge of huge public health ***-mediated synthesis of nanoparticles has recently gained attention as a cheap,rapid and eco-friendly method to control mosquito vector populations,with special reference to young ***,plant-fabricated nanoparticles have been successfully employed as dengue virus growth *** this Editorial,parasitologists,entomologists and researchers in drug nanosynthesis are encouraged to deal with a number of crucial challenges of public health importance.

关键词： Arbovirus Dengue Yellow fever Malaria Mosquito vectors nanobiotechnology

ZnO Nanoparticles: Synthesis and Adsorption Study

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Natural Science 2009年第2期1卷 129-135页

作者： K. Prasad Anal K. Jha 不详

A low-cost, green and reproducible probiotic microbe (Lactobacillus sporogens) mediated biosynthesis of ZnO nanoparticles is reported. The synthesis is performed akin to room tem-perature in five replicate samples. X-ray and transmission electron microscopy analyses are performed to ascertain the formation of ZnO nanoparticles. Rietveld analysis to the X-ray data indicated that ZnO nanoparticles have hexagonal unit cell structure. Individual nanoparticles having the size of 5-15 nm are found. A possible involved mechanism for the synthesis of ZnO nanoparticles has been pro-posed. The H2S adsorption characteristic of ZnO nanoparticles has also been assayed.

关键词： ZnO Nanoparticle Biosynthesis nanobiotechnology Eco-friendly H2S Adsorption