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Production of n-acetylglucosamine with Vibrio alginolyticus FA2,an emerging platform for economical unsterile open fermentation

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Synthetic and Systems Biotechnology 2023年第3期8卷 546-554页

作者： Yuan Peng Ping Xu Fei Tao State Key Laboratory of Microbial Metabolism Joint International Research Laboratory of Metabolic and Developmental Sciencesand School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghaiPeople’s Republic of China

Members of the Vibrionaceae family are predominantly fast-growing and halophilic microorganisms that have captured the attention of researchers owing to their potential applications in rapid *** them,Vibrio alginolyticus FA2 is a particularly noteworthy halophilic bacterium that exhibits superior growth *** has the potential to serve as a biotechnological platform for sustainable and eco-friendly open fermentation with *** evaluate this hypothesis,we integrated the n-acetylglucosamine(GlcnAc)pathway into *** *** nag genes were knocked out to obstruct the utilization of GlcnAc,and then 16 exogenous gna1s co-expressing with EcglmS were introduced to strengthen the flux of GlcnAc pathway,*** further enhance GlcnAc production,we fine-tuned promoter strength of the two genes and inactivated two genes alsS and alsD to prevent the production of ***,unsterile open fermentation was carried out using simulated seawater and a chemically defined medium,resulting in the production of 9.2 g/L GlcnAc in 14 *** is the first report for de-novo synthesizing GlcnAc with a Vibrio strain,facilitated by an unsterile open fermentation process employing seawater as a substitute for fresh *** development establishes a basis for production of diverse valuable chemicals using Vibrio strains and provides insights into biomanufacture.

关键词： Vibrio Open fermentation Metabolic engineering n-acetylglucosamine

Synergistic utilization of carbon sources for efficient biosynthesis of n-acetylglucosamine

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Green Chemical Engineering 2023年第2期4卷 233-238页

作者： Yanni Pei Yuhan Wang Xiaolin Shen Jia Wang Xinxiao Sun Qipeng Yuan State Key Laboratory of Chemical Resource Engineering Beijing University of Chemical TechnologyBeijing100029China

n-acetylglucosamine(GlcnAc)is an amino monosaccharide that has a variety of bioactivities and is widely used in pharmaceutical and food *** of GlcnAc by chitin hydrolysis is limited by the supply of raw materials and encounters the risk of shellfish protein *** efficient biosynthesis of GlcnAc,one challenge is to balance the carbon distribution between growth and ***,we applied the strategy of synergistic carbon utilization,in which glycerol supports cell growth and provides the acetyl group of GlcnAc while glucose serves as the precursor to *** efficiency of GlcnAc production was stepwise improved by blocking the product re-uptake and degradation,strengthening the biosynthetic pathway and synergistically utilizing two carbon *** these efforts,the final strain produced 41.5 g/L GlcnAc with a yield of 0.49 g/g of total carbon *** addition,we also explored the feasibility of using acetate as a cheap carbon source to partly replace *** study provides a promising alternative strategy for sustainable and efficient pro-duction of GlcnAc.

关键词： n-acetylglucosamine Synergistic carbon utilization Glucose facilitated diffusion system Acetate

Synthesis of two derivatives of tigogenyl disaccharides containing n-acetylglucosamine and the long range shielding effect of benzoyl groups

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Chinese Chemical Letters 2007年第4期18卷 373-376页

作者： Shu Jie Hou Liang Zhou Ping Sheng Lei De Quan Yu Key Laboratory of Bioactive Substance and Resources Utilization of Chinese Herbal Medicine Ministry of Education Institute of Materia Medica Peking Union Medical College ＆ Chinese Academy of Medical Sciences Beijing 100050 China

Two tigogenyl glycosides containing n-acetylglucosamine were synthesized. Their structures were confirmed by ^1H and ^13C nMR spectra. The shielding effect caused by benzoyl groups was elucidated by ^1H nMR, COSY, HSQ... 详细信息

关键词： n-acetylglucosamine Glycosylation Shielding effect

Metabolic engineering of Corynebacterium glutamicum S9114 based on whole-genome sequencing for efficient n-acetylglucosamine synthesis

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Synthetic and Systems Biotechnology 2019年第3期4卷 120-129页

作者： Chen Deng Xueqin Lv Yanfeng Liu Jianghua Li Wei Lu Guocheng Du Long Liu Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of EducationJiangnan UniversityWuxi214122China Key Laboratory of Industrial Biotechnology Ministry of EducationJiangnan UniversityWuxi214122China Shandong Runde Biotechnology CO. LTDTaian271200China

Glucosamine(Glcn)and its acetylated derivative n-acetylglucosamine(GlcnAc)are widely used in the pharmaceutical ***,we attempted to achieve efficient production of GlcnAc via genomic engineering of Corynebacterium ***,we ligated the GnA1 gene,which converts Glcn-6-phosphate to GlcnAc-6-phosphate by transferring the acetyl group in Acetyl-CoA to the amino group of Glcn-6-phosphate,into the plasmid pJYW4 and then transformed this recombinant vector into the *** ATCC 13032,ATCC 13869,ATCC 14067,and S9114 strains,and we assessed the GlcnAc titers at 0.5 g/L,1.2 g/L,0.8 g/L,and 3.1 g/L from each strain,*** suggested that there were likely to be significant differences among the key genes in the glutamate and GlcnAc synthesis pathways of these *** ***,we performed whole genome sequencing of the S9114 strain,which has not been previously published,and found that there are many differences among the genes in the glutamate and GlcnAc synthesis pathways among the four strains ***,nagA(encoding GlcnAc-6-phosphate deacetylase)and gamA(encoding Glcn-6-phosphate deaminase)were deleted in *** S9114 to block the catabolism of intracellular GlcnAc,leading to a 54.8%increase in GlcnAc production(from 3.1 to 4.8 g/L)when grown in a shaker *** addition,lactate synthesis was blocked by knockout of ldh(encoding lactate dehydrogenase);thus,further increasing the GlcnAc titer to 5.4 g/***,we added a key gene of the Glcn synthetic pathway,glmS,from different sources into the expression vector pJYW-4-cen,and the resulting recombinant strain CGGn2-GnA1-CgglmS produced the GlcnAc titer of 6.9 g/*** is the first report concerning the metabolic engineering of ***,and the results of this study provide a good starting point for further metabolic engineering to achieve industrial-scale production of GlcnAc.

关键词： C.glutamicum Complete genome n-acetylglucosamine Metabolic engineering

Synthesis of 4-[F-18]Fluoro-4-Deoxy-n-Acetyl-1,3,6-Tri-O-acetylglucosamine, a Potential Brain Imaging PET Agent

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natural Science 2024年第3期16卷 19-23页

作者： Murthy Akula Derek Cressy Lee Collier George Kabalka Dustin Osborne Department of Radiology The University of Tennessee Medical Center Knoxville USA Advion Biosciences Ithaca USA

The synthesis of a promising brain imaging agent 4-[F-18]fluoro-4-deoxy-n-acetyl-1,3,6-tri-O-acetylglucosamine, 2, was successfully accomplished from commercially available n-acetyl glucosamine in 5 steps. The non-decay corrected radiochemical yield and purity were found to be 31% ± 4% (n = 3) and >98% respectively. The total reaction time for radio labelling step was 50 min.

关键词： n-acetylglucosamine Amyloidogenesis Lipase-Candida rugosa

Systematic analyses of glutamine and glutamate metabolisms across different cancer types

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Chinese Journal of Cancer 2017年第12期36卷 712-725页

作者： Yuan Tian Wei Du Sha Cao Yue Wu ning Dong Yan Wang Ying Xu College of Computer Science and Technology Jilin UniversityChangchun 130012JilinP.R.China The First Hospital Jilin UniversityChangchun 130012JilinP.R.China College of Public Health Jilin UniversityChangchun 130012JilinP.R.China Computational Systems Biology Lab Department of Biochemistry and Molecular Biology and Institute of BioinformaticsUniversity of Georgia120 E Green StAthensGA 30602USA

Background: Glutamine and glutamate are known to play important roles in cancer biology. However, no detailed information is available in terms of their levels of involvement in various biological processes across different cancer types, whereas such knowledge could be critical for understanding the distinct characteristics of different cancer types. Our computational study aimed to examine the functional roles of glutamine and glutamate across different cancer ***: We conducted a comparative analysis of gene expression data of cancer tissues versus normal control tissues of 11 cancer types to understand glutamine and glutamate metabolisms in cancer. Specifically, we developed a linear regression model to assess differential contributions by glutamine and/or glutamate to each of seven biological processes in cancer versus control ***: While our computational predictions were consistent with some of the previous observations, multiple novel predictions were made:(1) glutamine is generally not involved in purine synthesis in cancer except for breast cancer, and is similarly not involved in pyridine synthesis except for kidney cancer;(2) glutamine is generally not involved in ATP production in cancer;(3) glutamine's contribution to nucleotide synthesis is minimal if any in cancer;(4) glutamine is not involved in asparagine synthesis in cancer except for bladder and lung cancers; and(5) glutamate does not contribute to serine synthesis except for bladder ***: We comprehensively predicted the roles of glutamine and glutamate metabolisms in selected metabolic pathways in cancer tissues versus control tissues, which may lead to novel approaches to therapeutic development targeted at glutamine and/or glutamate metabolism. However, our predictions need further functional validation.

关键词： Glutamine metabolism Glutamate metabolism nucleotide synthesis Lipid synthesis Uridine diphosphate n-acetylglucosamine (UDP-GlcnAc) metabolism