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The genetic diversity analysis in the donkey myostatin gene

Journal of Integrative Agriculture 2017年第3期16卷 656-663页

作者： LIU Dong-hua HAN Hao-yuan ZHANG Xin SUN Ting LAN Xian-yong CHEN Hong LEI Chu-zhao DANG Rui-hua College of Animal Science and Technology Northwest A&F University Institute of Hexi Ecology and Oasis Agriculture Hexi University

myostatin（MSTN）gene negatively controls skeletal muscle development and growth,variations of which play an important role in the regulation of skeletal muscle growth in ***,study on genetic polymorphism of MSTN gene in donkey is *** this study,we screened the single nucleotide polymorphsims（SNPs）of MSTN gene in 13 Chinese donkey *** novel SNPs（g.229T〉C,g.872A〉G,g.2014G〉A,and g.2395C〉G）were detected and genotyped by sequencing and polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）*** haplotypes（H1–H6）were analyzed,which indicated abundant haplotype diversities in Chinese *** haplotype H1 was the most dominant and ancient in all *** donkey displayed the highest haplotype *** Neighbour-Joining（NJ）tree of MSTN gene among different species was *** clustering result of nine species was consistent with the fact of species *** results will provide a reliable theoretical basis for the preservation,exploration and utilization of Chinese donkey genetic resources.

关键词： Chinese donkey myostatin gene SNP genetic diversity

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Sequencing Analysis of myostatin gene （MSTN） for Meat Cattle in Mongolia

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Journal of Agricultural Science and Technology(A) 2016年第6期6卷 429-434页

作者： Undarmaa Budragchaa Munkhtogtokh Baljinnyam Davaakhuu Luvsan Sergelen Baldan Nyamsuren Balsai Sodnom Lkhagvasuren School of Husbandry and Technology Mongolian University of Life Science (MULS) Zaisan 17024 Ulaanbaatar Mongolia Research Institute of Animal Husbandry Mongolian University of Life Science (MULS) Zaisan 17024 Ulaanbaatar Mongolia Institute of Veterinary Medicine Mongolian University of Life Science (MULS) Zaisan 17024 Ulaanbaatar Mongolia

In developed countries, study on special or candidate genes, which are useful for identifying species, breed and productivity of livestock, was conducted at high level and the results have already been used in practice. Such advanced technology and innovation that we are facing is necessary to adopt in Mongolia. In this study, the myostatin gene （MSTN） was investigated as a candidate gene for meat animal in Mongolian breeds of cattle. The conventional phenol-chloroform method and FavorPrepTM tissue DNA extraction kit were used for DNA isolation, and the polymerase chain reaction （PCR） and sequencing analysis were used for further study. The nucleotide sequences of MSTN gene from Selenge, Kazakh white head breeds and Mongolian cattle were sequenced and reported on the DDBJ/EMBL/GenBank database （LC142726, LC146648, LC146649）, and Selenge breed showed the result of single nucleotide mutation in MSTN gene.

关键词： Meat cattle candidate gene myostatin gene sequencing.

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Cloning and Sequence Analysis on 3′ Coding Region of Wild Boar and Cross Bred Pig myostatin gene

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Journal of Northeast Agricultural University(English Edition) 2004年第2期11卷 148-150页

作者： LIUDi YANGXiu-qin YANGJia-fang FacultyofAnimalScienceandTechnology NortheastAgriculturalUniversityHarbinHeilongjiang150030PRC／／HeilongjiangAgriculturalAcademyHarbinHeilongjiang150086PRC FacultyofAnimalScienceandTechnology NortheastAgriculturalUniversityHarbinHeilongjiang150030PRC

myostatin, with a highly conservative gene among breeds is a negative regulator of muscle. The 3′ coding regions of wild boar and crossbred pig myostatin were cloned by RT-PCR and sequenced respectively. The homology of the nucleotide sequence between wild boar and crossbred pig was 100% and there was no difference in this region compared with pig myostatin gene of Genbank. This indicated that there was not change of gene sequence in this region during the evolution processes.

关键词： myostatin gene RT-PCR 3'coding regions

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Study on the Polymorphisms of Porcine myostatin gene in Promoter Region by PCR-RFLPS

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Journal of Northeast Agricultural University(English Edition) 2005年第1期12卷 65-67页

作者： YANGXiu-qin LIUDi FacultyofAnimalScienceandTechnology NortheastAgriculturalUniversityHarbinHeilongjiang150030PRC FacultyofAnimalScienceandTechnology NortheastAgriculturalUniversityHarbinHeilongjiang150030PRC／／AgriculturalAcademyofHeilongjiangProvinceHarbinHeilongjiang150086PRC

In order to further study functions of the porcine myostatin gene, we analyzed the polymorphisms of porcine myostatin gene in promoter region among different breeds including Yorkshire, Landrace, Duroc, Junmu, Min pig and Sanjiang white pig by PCR-RFLPs. The allele T dominated in the imported lean-type pig breeds such as Yorkshire, Landrace and Duroc. No allele A was detected in Junmu and Sanjiang white pig, and the frequencies of three genotypes were about equal in Min pig. The result using X2 analysis showed that the distribution of three genotypes was related to pig breeds.

关键词： polymorphisms PCR-RFLPS porcine myostatin gene

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genetic Variation Of myostatin Promoter In Nanyang Cattle And Yak

Genetic Variation Of Myostatin Promoter In Nanyang Cattle An...

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第十次全国畜禽遗传标记研讨会

作者： ZHANG Run-feng, CHEN Hong, ZHANG Zheng-feng, ZHANG Hai-jun, YAN Lin-jun, BAO Bin (College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou, Jiangsu 221116, China)

myostatin (GDF-8) acts as a negative regulator of muscle growth. Mutations of the gene are responsible for the double muscling phenotype in several European cattle breeds. In order to further study functions of the bovine myostatin gene, the polymorphisms at promoter region of bovine myostatin gene were detected in Nanyang breed and yak by PCR-RFLPs. The frequencies of the SNP at -371 (T/A) ( relative to ATG translation start codon) were assessed. The allele T dominated in Nanyang breed and yak. No allele A was detected in yak and no homozygous individual with genotype AA was found in Nanyang breed.

关键词： myostatin gene SNP Nanyang cattle Yak PCR-RFLP

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