检索结果-南通市图书馆

myeloid-derived suppressor cells Activate Liver Natural Killer cells in a Murine Model in Uveal Melanoma

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Current Medical Science 2022年第5期42卷 1071-1078页

作者： Yuan-yuan WANG Shuang-ying LI San-qian CHEN Liang-liang WANG Zhi-qiang HAN Department of Obstetrics and Gynecology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Department of Obstetrics and Gynecology Chibi People’s HospitalChibi437300China

Objective Elevated myeloid-derived suppressor cells(MDSCs)in many malignancies are associated with the increased risk for metastases and poor ***,a mouse model of intraocular melanoma was established to explore how MDSCs influence liver *** In this study,murine B16LS melanoma cells were transplanted into the posterior compartment(PC)of the eye of C57BL/6 *** from the liver of naive mice and mice bearing melanoma liver metastasis were isolated using isotonic Percoll centrifugation,examined by flow cytometry for their expression of Gr1,CD11b,F4/80,RAE-1,and Mult-1,and further isolated for MDSCs and natural killer(NK)*** effects of MDSCs on NK cells were tested by coculturing and assessing the ability of NK cells to produce interferon-gamma(IFN-γ)by ELISA and NK cell cytotoxicity by 3H-thymidine incorporation *** impact of IFN-γon liver metastases was examined via selectively depleting IFN-γin *** The results showed that mice with liver metastases had increased levels of CD11b+Gr1+F4/80+as well as CD11b+Gr1+F4/80−*** significantly enhanced the generation of IFN-γtogether with the cytotoxicity of the NK ***,these effects were cell-cell *** IFN-γwas not of a toxic nature to the melanoma cells,it profoundly inhibited B16LS cell *** IFN-γin vivo led to increased liver *** All these findings first revealed that MDSCs accumulated in liver metastasis of intraocular melanoma could activate the NK cells to produce an effective anti-tumor immune ***,the MDSCs’performance in different tumor models would need more investigation to boost current immunotherapy modalities.

关键词： myeloid-derived suppressor cells natural killer cells IFN-γ liver metastases

myeloid-derived suppressor cells in cancer

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

iLIVER 2022年第2期1卷 81-89页

作者： Jun Gao Wei-Ren Liu Zheng Tang Jia Fan Ying-Hong Shi Department of Liver Surgery and Transplantation Liver Cancer InstituteZhongshan HospitalFudan UniversityShanghaiChina Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education ShanghaiChina Institutes of Biomedical Sciences Fudan UniversityShanghaiChina Shanghai Key Laboratory of Organ Transplantation ShanghaiChina State Key Laboratory of Genetic Engineering and Collaborative Innovation Center for Genetics and Development School of Life SciencesFudan UniversityShanghaiChina

Numerous recent studies have shown that myeloid-derived suppressor cells(MDSCs),a strongly heterogeneous population of immunosuppressive cells,are dysregulated in the presence of many *** present different phenotypes and play prominent roles in the tumor *** date,gene therapies targeting MDSCs are the most innovative and flexible methods to specifically modify the tumor ***,we summarize current studies related to the phenotypes,functions,and mechanisms of MDSCs and explore the therapeutic landscape of chemokines that affect the balance between subpopulations of MDSCs.

关键词： myeloid-derived suppressor cells Immunosuppression Immunostimulatory Tumor microenvironment Targeted therapy

Contribution of myeloid-derived suppressor cells to tumor-induced immune suppression,angiogenesis,invasion and metastasis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2010年第7期37卷 423-430页

作者： Xian-Zong Ye Shi-Cang Yu Xiu-Wu Bian Institute of Pathology and Southwest Cancer Center Southwest Hospital Third Military Medical University Chongqing 400038 China

Growing evidence suggests that myeloid-derived suppressor cells （MDSCs）,which have been named ＂immature myeloid cells＂ or ＂myeloid suppressor cells＂（MSCs）,play a critical role during the progression of cancer in tumor-bearing mice and cancer *** their name implies,these cells are derived from bone marrow and have a tremendous potential to suppress immune *** studies indicated that these cells also have a crucial role in tumor *** can directly incorporate into tumor *** secret many pro-angiogenic factors as *** addition,they play an essential role in cancer invasion and metastasis through inducing the production of matrix metalloproteinases （MMPs）,chemoattractants and creating a pre-metastatic *** evidence supports the idea that cancer stem cells （CSCs） are responsible for tumorigenesis,resistance to therapies,invasion and ***,we hypothesize that CSCs may ＂hijack＂ MDSCs for use as alternative niche cells,leading to the maintenance of stemness and enhanced chemo-and radio-therapy *** countermeasure that directly targets to MDSCs may be useful for against angiogenesis and preventing cancer from invasion and ***,the study of MDSCs is important to understand tumor progression and to enhance the therapeutic efficacy against cancer.

关键词： myeloid-derived suppressor cells immune suppression angiogenesis invasion metastasis

Circulating myeloid-derived suppressor cells in patients with pancreatic cancer

在线全文

学校读者我要写书评

暂无评论

Hepatobiliary & Pancreatic Diseases International 2016年第1期15卷 99-105页

作者： Xiao-Dong Xu Jun Hu Min Wang Feng Peng Rui Tian Xing-Jun Guo Yu Xie Ren-Yi Qin Department of Biliary-Pancreatic Surgery Affiliated Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China Department of Colon Cancer Tianjin Medical University Cancer Institute and Hospital National Clinical Research Center of Cancer Key Laboratory of Cancer Prevention and TherapyTianjin 300060 China

BACKGROUND： myeloid-derived suppressor cells （MDSCs） are heterogeneous cell types that suppress T-cell responses in cancer patients and animal models, some MDSC subpopulations are increased in patients with pancreatic cancer. The present study was to investigate a specific subset of MDSCs in patients with pancreatic cancer and the mechanism of MDSCs increase in these patients. METHODS： myeloid cells from whole blood were collected from 37 patients with pancreatic cancer, 17 with cholangiocarcinoma, and 47 healthy controls. Four pancreatic cancer cell lines were co- cultured with normal peripheral blood mononudear cells （PBMCs） to test the effect of tumor cells on the conversion of PBMCs to MDSCs. Levels of granulocyte-macrophage colony-stimulating factor （GM-CSF） and arginase activity in the plasma of cancer patients were analyzed by enzyme-linked immunosorbent assay. RESULTS： CD14＋/CD11b＋/HLA-DR MDSCs were increased in patients with pancreatic or bile duct cancer compared with those in healthy controls, and this increase was correlated with clinical cancer stage. Pancreatic cancer cell lines induced PBMCs to MDSCs in a dose-dependent manner. GM-CSF and arginase activity levels were significantly increased in the se rum of patients with pancreatic cancer. CONCLUSIONS： MDSCs were tumor related： tumor cells induced PBMCs to MDSCs in a dose-dependent manner and circulating CD14＋/CD11b＋/HLA-DR- MDSCs in pancreatic cancer patients were positively correlated with tumor burden. MDSCs might be useful markers for pancreatic cancer detection and progression.

关键词： pancreatic cancer myeloid-derived suppressor cells granulocyte- macrophage colony-stimulating factor arginase

ACAT1 deficiency in myeloid cells promotes glioblastoma progression by enhancing the accumulation of myeloid-derived suppressor cells

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2023年第12期13卷 4733-4747页

作者： Mingjin Wang Weida Wang Shen You Zhenyan Hou Ming Ji Nina Xue Tingting Du Xiaoguang Chen Jing Jin Department of Pharmacology State Key Laboratory of Bioactive Substances and Functions of Natural MedicinesInstitute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China

Glioblastoma(GBM)is a highly aggressive and lethal brain tumor with an immunosuppressive tumor microenvironment(TME).In this environment,myeloid cells,such as myeloid-derived suppressor cells(MDSCs),play a pivotal role in suppressing antitumor *** is closely related to the function of myeloid ***,our study reports that acetyl-CoA acetyltransferase 1(ACAT1),the key enzyme of fatty acid oxidation(FAO)and ketogenesis,is significantly downregulated in the MDSCs infiltrated in GBM *** investigate the effects of ACAT1 on myeloid cells,we generated mice with myeloid-specific(LyzM-cre)depletion of *** results show that these mice exhibited a remarkable accumulation of MDSCs and increased tumor progression both ectopically and *** mechanism behind this effect is elevated secretion of C-X-C motif ligand 1(CXCLI)of macrophages(Mo).Overall,our findings demonstrate that ACAT1 could serve as a promising drug target for GBM by regulating the function of MDSCs in the TME.

关键词： Glioblastoma myeloid cells myeloid-derived suppressor cells Acetyl-CoA acetyltransferase 1 CXCL1 Tumor microenvironment Lipid metabolism Macrophages

Cyclooxygenase-2 Blockade Inhibits Accumulation and Function of myeloid-derived suppressor cells and Restores T Cell Response after Traumatic Stress

在线全文

学校读者我要写书评

暂无评论

Journal of Huazhong University of Science and Technology(Medical Sciences) 2014年第2期34卷 234-240页

作者：李仁杰刘琳高伟宋先舟白祥军李占飞 Department of Traumatic Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Department of Biochemistry and Molecular Biology Tongji Medical College Huazhong University of Science and Technology

myeloid-derived suppressor cells （MDSCs） play a crucial role in T cell dysfunction, which is related to poor outcome in patients with severe trauma. Cyclooxygenase-2 （Cox-2） contributes to immune disorder in trauma and infection via production of prostaglandin E2. However, the role of Cox-2 in the accumulation and function of MDSCs after traumatic stress has not been fully elucidated. In the present study, we treated murine trauma model with NS398, a selective Cox-2 inhibitor. Then the percentages of CD1 lb＋/Gr-l＋ cells, proliferation and apoptosis of CD4＋ T cells were determined. Ar- ginase activity and arginase-1 （Arg-1） protein expression of splenic CDllb＋/Gr-I＋ cells, and de- layed-type hypersensitivity （DTH） response were analyzed. The results showed that Cox-2 blockade significantly decreased the percentages of CD1 lb＋/Gr-l＋ cells in the spleen and bone marrow 48 and 72 h after traumatic stress. NS398 inhibited arginase activity and down-regulated the Arg-1 expression of splenic CD1 lb＋/Gr-l＋ ceils. Moreover, NS398 could promote proliferation and inhibit apoptosis of CD4＋ T cells. It also restored DTH response of traumatic mice. Taken together, our data revealed that Cox-2 might play a pivotal role in the accumulation and function of MDSC after traumatic stress.

关键词： myeloid-derived suppressor cells trauma T cell dysfunction cyclooxygenase-2

The role of myeloid-derived suppressor cells in gastrointestinal cancer

在线全文

学校读者我要写书评

暂无评论

Cancer Communications 2021年第6期41卷 442-471页

作者： Cheng Cui Penglin Lan Li Fu Guangdong Provincial Key Laboratory of Regional Immunity and Diseases Department of Pharmacology and International Cancer CentreShenzhen University School of MedicineShenzhenGuangdong 518055P.R.China

Gastrointestinal(GI)cancer encompasses a range ofmalignancies that originate in the digestive system,which together represent the most common form of cancer diagnosed ***,despite numerous advances in both diagnostics and treatment,the incidence and mortality rate of GI cancer are on the ***-derived suppressor cells(MDSCs)are a heterogeneous population of immature myeloid cells that increase in number under certain pathological conditions,such as infection and inflammation,and this expansion is of particular relevance to *** are heavily involved in the regulation of the immune system and act to dampen its response to tumors,favoring the escape of tumor cells from immunosurveillance and increasing both metastasis and *** recent studies have supported the use of MDSCs as a prognostic and predictive biomarker in patientswith cancer,and potentially as a novel treatment *** the present review,the mechanisms underlying the immunosuppressive functions of MDSCs are described,and recent researches concerning the involvement of MDSCs in the progression,prognosis,and therapies of GI cancer are *** aim of this work was to present the development of novel treatments targeting MDSCs in GI cancer in the hope of improving outcomes for patients with this condition.

关键词： gastrointestinal cancer tumor immunology myeloid-derived suppressor cells progression prognosis therapy

Amplification of Functional myeloid-derived suppressor cells during Stem Cell Mobilization Induced by Granulocyte Colony-stimulation-factor

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Huazhong University of Science and Technology(Medical Sciences) 2013年第6期33卷 817-821页

作者：朱晓健胡静孙立肖音陈智超游泳邹萍王红祥仲照东 Institute of Hematology Union HospitalTongji Medical CollegeHuazhong University of Science and Technology Department of Hematology Ningbo Medical Treatment Center Department of Hematology Wuhan Central Hospital

The effects of granulocyte colony-stimulation-factor （G-CSF） on stem cell mobilization and its impact on the amplification of myeloid-derived suppressor cells （MDSCs） of donor mice were ex- amined. A mouse model of stem cell mobilization was established by consecutive subcutaneous injec- tion of 100 μg/kg G-CSF for 5 days. The blood from the donor mice was routinely examined during mobilization. Stem cells and MDSCs were analyzed by flow cytometry. The immunosuppressive mole- cules derived from MDSCs in serum and spleen, including hydrogen dioxide （H202） and nitric oxide （NO）, and the activity of nitric oxide synthase （NOS） were determined during the mobilization. Apop- tosis of T lymphocytes was assessed by using Annexin-V/PI. During stem cell mobilization, the number of lymphocytes and white blood cells in the peripheral blood was increased, and peaked on the 4th day. The number of stem cells in G-CSF-treated mice was significantly greater than that in controls （P〈0.01）. The expansions of MSDCs were also observed after G-CSF mobilization, with a more notable rate of growth in the peripheral blood than in the spleen. The activity of NOS and the production of NO were increased in the donor mice, and the serum H202 levels were approximately 4-fold greater than the con- trois. Consequently, apoptosis of T lymphocytes was increased and showed a positive correlation with the elevated percentage of MDSCs. It was concluded that G-CSF could provide sufficient peripheral blood stem cells for transplantation. Exogenous administration of G-CSF caused the accumulation of MDSCs in the peripheral blood and the spleen, which could lead to apoptosis ofT lymphocytes and may offer a new strategy for the prevention and treatment of graft versus host disease.

关键词： myeloid-derived suppressor cells granuloeyte colony-stimulation-factor stem cell mobili- zation transplantation immunosuppressive molecules

myeloid-derived suppressor cells(MDSCs) recruited and activated by chemokine CCL5 and its receptor CCR1 is crucial for pathogenic progression of endometriosis

在线全文

学校读者我要写书评

暂无评论

Myeloid-derived suppressor cells(MDSCs) recruited and activa...