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Zeptomole Imaging of Cytosolic MicroRNA Cancer Biomarkers with A Light-Controlled Nanoantenna

Nano-Micro Letters 2021年第12期13卷 468-482页

作者： Yang Song Xiaoli Cai Grayson Ostermeyer Shichao Ding Dan Du Yuehe Lin School of Mechanical and Materials Engineering Washington State UniversityPullmanWA 99164USA Nanosong Systems LLC RedmondWA 98052USA School of Biological Sciences Washington State UniversityPullmanWA 99164USA

Detecting and quantifying intracellular microRNAs(miRNAs)are a critical step in resolving a cancer diagnostic and resolving the ensemble of gene products that orchestrate the living state of ***,the nanoprobe for detecting low abundance miRNAs in cell cytosol is restricted by either the"one-to-one"signaltrigger model or di culty for cytosol *** address these challenges,we designed a lightharvesting nanoantenna-based nanoprobe,which directs excitation energy to a single molecule to sensitively detect cytosolic *** light irradiation,the light-harvesting nanoantenna e ectively disrupted lysosomal structures by generationof reactive oxygen species,substantially achieved cytosol *** nanoantenna containing>4000 donor dyes can e ciently transfer excitation energy to one or two acceptors with 99%e ciency,leading to unprecedented signal amplification and biosensing *** designed nanoantenna can quantify cytosolic miR-210 at zeptomolar *** fluorescence lifetime of the donor exhibited good relationship with miR-210 concentration in the range of 0.032 to 2.97 amol/*** zeptomole sensitivity of nanoantenna provides accurate bioimaging of miR-210 both in multiple cell lines and in vivo assay,which creates a pathway for the creation of miRNA toolbox for quantitative epigenetics and personalized medicine.

关键词： Light-harvest Nanoantenna miRNA detection Zeptomole multiple cell lines

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Three-dimensional bioprinting is not only about cell-laden structures

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Chinese Journal of Traumatology 2016年第4期19卷 187-192页

作者： Hong-Bo Zhang Tian-Long Xing Rui-Xue Yin Yong Shi Shi-Mo Yang Wenqun Zhang Complex and Intelligent Research Centre School of Mechanical and Power Engineering East China University of Sdence and Technology Shanghai China Division of Biomedical Engineering University of Saskatchewan Saskatoon SK STN 5A9 Canada

In this review, we focused on a few obstacles that hinder three-dimensional (3D) bioprinting process in tissue engineering. One of the obstacles is the bioinks used to deliver cells. Hydrogels are the most widely used bioink materials; however, they aremechanically weak in nature and cannot meet the requirements for supporting structures, especially when the tissues, such as cartilage, require extracellular matrix to be mechanically strong. Secondly and more importantly, tissue regeneration is not only about building all the components in a way that mimics the structures of living tissues, but also about how to make the constructs function normally in the long term. One of the key issues is sufficient nutrient and oxygen supply to the engineered living constructs. The other is to coordinate the interplays between cells, bioactive agents and extracellular matrix in a natural way. This article reviews the approaches to improve the mechanical strength of hydrogels and their suitability for 3D bioprinting; moreover, the key issues of multiple cell lines coprinting with multiple growth factors, vascularization within engineered living constructs etc. were also reviewed.

关键词： 3D bioprinting Hydrogels Vascularization multiple cell lines

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