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Genetic Diversity, Antimicrobial Resistance, and Virulence Genes of Aeromonas Isolates from Clinical Patients, Tap Water Systems, and Food

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Biomedical and Environmental Sciences 2020年第6期33卷 385-395页

作者： MENG Shuang WANG Yong Lu LIU Chen Geng YANG Jing YUAN Min BAI Xiang Ning JIN Dong LIANG Jun Rong CUI Zhi Gang LI Juan State Key Laboratory of Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 10026China Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Zhejiang 310058HangzhouChina Ma'anshan Center for Disease Control and Prevention Ma'anshan 243031AnhuiChina Clinical Laboratoty of Xuanwu Hospital Captital Medcial University'Beijing 100053China

Objective This study aimed to evaluate the genetic diversity,virulence,and antimicrobial resistance of Aeromonas isolates from clinical patients,tap water systems,and *** Ninety Aeromonas isolates were obtained from Ma’anshan,Anhui province,China,and subjected to multi-locus sequence typing(MLST)with six housekeeping *** taxonomy was investigated using concatenated gyr B-cpn60 sequences,while their resistance to 12 antibiotics was *** putative virulence factors and several resistance genes were identified by PCR and *** The 90 Aeromonas isolates were divided into 84 sequence types,80 of which were novel,indicating high genetic *** Aeromonas isolates were classified into eight different *** assays identified virulence genes in the isolates,with the enterotoxin and hemolysin genes act,aer A,alt,and ast found in 47(52.2%),13(14.4%),22(24.4%),and 12(13.3%)of the isolates,*** majority of the isolates(≥90%)were susceptible to aztreonam,imipenem,cefepime,chloramphenicol,gentamicin,tetracycline,and ***,several resistance genes were detected in the isolates,as well as a new mcr-3 *** sequence type,virulence properties,and antibiotic resistance vary in Aeromonas isolates from clinical patients,tap water systems,and food.

关键词： Aeromonas multi-locus sequence typing multidrug resistance Virulence gene Antimicrobial resistance gene

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Surveillance and examination of microbial contamination in ice cream in China

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Food Quality and Safety 2022年第3期6卷 446-453页

作者： Lin Yan Xiaoyan Pei Junjie Miao Ying Li Shuran Yang Zixin Peng Xiaorong Yang Lingling Mei Qingwen Yang Huan Ren Dajin Yang Hongmei Shi Hebei Key Laboratory of Environment and Human Health School of Public HealthHebei Medical UniversityShijiazhuangChina China National Center for Food Safety Risk Assessment(CFSA) BeijingChina Sichuan Provincial Center for Disease Control and Prevention ChengduChina Zhejiang Provincial Center for Disease Control and Prevention HangzhouChina Yunnan Provincial Center for Disease Controland Prevention KunmingChina

This study investigated the microbial contamination of ice cream in China.A total of 2887 ice cream samples were collected from different regions of *** by the aerobic plate count(APC),coliforms,and three foodborne pathogens,Listeria monocytogenes(***),Staphylococcus aureus(***),and Salmonella spp.,was detected in the *** isolates were further analyzed for antibiotic susceptibility and multi-locus sequence typing(MLST).The results showed that APC was more than 10^(5) colony forming units(CFU)/g in 6.10%(176/2887)and coliform was more than 10^(2) CFU/g in 15.69%(453/2887)of all *** positive rates of ***,***,and Salmonella *** 0.66%,0.62%,and 0.10%,*** these,*** contamination was more than 102 CFU/g in two samples,and *** in the positive sample was in the range of 0.3-240 most probable number(MPN)/*** a median of 4.3 MPN/*** hygienic status of the packaged samples was much better than that of the bulk samples(P0.05).For 18 *** strains isolated from ice cream,the resistance rate of nine antibiotics was 5.56%(1/18).By MLST,the *** strains were classified into nine sequence types(STs),of which ST8 was the most common(six isolates).These results indicate that a potential health risk to the public may be caused by ice cream,particularly in susceptiblepopulations.

关键词： Frozen dessert microbial contamination foodborne pathogens antibiotic susceptibility multi-locus sequence typing

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Disease burden,antimicrobial resistance and molecular characterization of invasive group B Streptococcus among non-pregnant adults in Malaysia:A protocol study

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Asian Pacific Journal of Tropical Medicine 2023年第11期16卷 498-505页

作者： Abdulrahman Muthanna Nurul Hana Zainal Baharin Mohd Nasir Mohd Desa Nurul Diana Dzaraly Mohammad Noor Amal Azmai Syafinaz Amin-Nordin Department of Biomedical Sciences Faculty of Medicine and Health SciencesUniversiti Putra Malaysia43400 SerdangSelangorMalaysia Department of Biology Faculty of ScienceUniversiti Putra Malaysia43400 SerdangSelangorMalaysia Aquatic Animal Health and Therapeutics Laboratory Institute of BioscienceUniversiti Putra Malaysia43400 SerdangSelangorMalaysia Department of Medical Microbiology Faculty of Medicine and Health SciencesUniversiti Putra Malaysia43400 SerdangSelangorMalaysia Department of Medical Microbiology Sultan Abdul Aziz Shah HospitalUniversiti Putra MalaysiaPersiaran MARDI-UPM43400SelangorMalaysia Laboratory of Vaccine and Biomolecules Institute of BioscienceUniversiti Putra Malaysia43400 SerdangSelangorMalaysia

and pili genes are also ***:This multicentre,prospective,observational study is conducted in seven major tertiary hospitals in Malaysia among non-pregnant ***,a retrospective study is conducted in the selected hospitals with similar *** isolates are subjected to phenotyping,serotyping by multiplex PCR,antimicrobial susceptibility testing and PCR-detection of GBS virulence and pilus *** housekeeping genes are amplified and sequenced for multi-locus sequence ***:Findings from the study may contribute to the management of clinical practice to diagnose and prevent GBS related diseases in a timely *** use of antibiotics is encouraged by monitoring antimicrobial resistance.

关键词： Group B Streptococcus Incidence Serotype Antimicrobial resistance Virulence gene multi-locus sequence typing

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Resolving the Mortierellaceae phylogeny through synthesis of multi-gene phylogenetics and phylogenomics

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Fungal Diversity 2020年第5期104卷 267-289页

作者： Natalie Vandepol Julian Liber Alessandro Desirò Hyunsoo Na Megan Kennedy Kerrie Barry Igor V.Grigoriev Andrew N.Miller Kerry O’Donnell Jason E.Stajich Gregory Bonito Department of Microbiology and Molecular Genetics Michigan State UniversityEast LansingMI 48824USA Department of Plant Biology Michigan State UniversityEast LansingMI 48824USA Department of Plant Soil and Microbial Sciences Michigan State UniversityEast LansingMI 48824USA Joint Genome Institute BerkeleyCA 94720USA Illinois Natural History Survey University of Illinois Urbana-ChampaignChampaignIL 61820USA United States Department of Agriculture Agricultural Research ServicePeoriaIL 61604USA Department of Microbiology and Plant Pathology&Institute for Integrative Genome Biology University of California-RiversideRiversideCA 92521USA

Early efforts to classify Mortierellaceae were based on macro-and micromorphology,but sequencing and phylogenetic studies with ribosomal DNA(rDNA)markers have demonstrated conflicting taxonomic groupings and polyphyletic *** some taxonomic confusion in the family has been clarified,rDNA data alone is unable to resolve higher level phylogenetic relationships within *** this study,we applied two parallel approaches to resolve the Mortierel-laceae phylogeny:low coverage genome(LCG)sequencing and high-throughput,multiplexed targeted amplicon sequenc-ing to generate sequence data for multi-gene *** then combined our datasets to provide a well-supported genome-based phylogeny having broad sampling depth from the amplicon *** the Mortierellaceae phylogeny into monophyletic genera resulted in 13 genera,7 of which are newly ***-coverage genome sequencing proved to be a relatively cost-effective means of generating a high-confidence *** multi-gene phylogenetics approach enabled much greater sampling depth and breadth than the LCG approach,but has limitations *** present this work to resolve some of the taxonomic confusion and provide a genus-level framework to empower future studies on Mortierellaceae diversity and evolution.

关键词： Mortierellaceae Phylogenomics Molecular systematics Taxonomy multi-locus sequence typing

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Antimicrobial resistance and molecular typing of Salmonella in the chicken production chain in Hubei Province, China

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Animal Diseases 2023年第3期3卷 188-201页

作者： Cuirong Wu Lihui Liu Yuxin Sui Lulu Huang Yang Chen Haihong Hao Yuanhu Pan Zhenli Liu Guyue Cheng National Reference Laboratory of Veterinary Drug Residues(HZAU)and MAO Key Laboratory for Detection of Veterinary Drug Residues Huazhong Agricultural UniversityWuhanHubeiChina Department of Animal Husbandry and Veterinary Medicine Chengdu Agricultural CollegeChengduSichuanChina MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products Huazhong Agricultural UniversityWuhanHubeiChina

Salmonella is a significant foodborne zoonotic pathogen that endangers both human and animal *** goal of this research is to gain a preliminary understanding of Salmonella contamination and antimicrobial resistance in the chicken production chain in Hubei Province,China.1149 animal and environmental samples were collected from chicken farms,slaughterhouses,and retail markets in six cities across Hubei Province in China from 2019 to 2020,yielding Salmonella isolation rates of 4.68%(28/598),12.21%(47/385),and 9.64%(16/166),*** distinct serotypes were detected among 53 non-clonal Salmonella strains,of which Meleagridis(26.42%,14/53)was the dominant *** half of the strains(49.06%,26/53)were multi-drug resistant(MDR).Whole-genome sequencing(WGS)showed that 10 resistance genes(tetA,bla_(TEM),parC,qnrs1,floR,aac(6'-ly,aph(6)-ld,aph(3")-b,aac(6')-laa and sul2)and 7 categories of virulence genes were present in all three links in 22 non-clonal dominant serotype *** was shown that Salmonella in the chicken production chain in Hubei Province had a high resist-ance rate to Tetracycline(TET,73.58%),Ofloxacin(OFL,69.81%),Florfenicol(FFC,60.38%)and Ampicillin(AMP,39.62%)which was consistent with the widespread use of these drugs in the husbandry industry in *** ST types determined by MLST and serotypes determined by WGS had a one-to-one *** spanning tree analysis revealed that there was cross contamination of Salmonella in farms and slaughterhouses,slaughterhouses and markets,animal samples and environmental *** work provides useful information for the prevention and control of contamination and antimicrobial resistance of Salmonella in the chicken production chain,as well as demonstrating the dependable role of WGS in Salmonella molecular typing.

关键词： Salmonella Serotype Antimicrobial resistance multi-locus sequence typing Whole genome sequencing

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