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Morphological comparison and molecular marker screening of three Skeletonema species found in Changjiang(Yangtze)River Basin

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Journal of Oceanology and Limnology 2024年第3期42卷 923-942页

作者： Jingwen HU Yuxin YI Zhengxin YANG John Patrick KOCIOLEK Pan YU Quanxi WANG Qingmin YOU College of Life Sciences Shanghai Normal UniversityShanghai 200235China Museum of Natural History University of ColoradoBoulder 80309USA College of Environmental and Geographical Sciences Shanghai Normal UniversityShanghai 200235China

In our recent investigations of diatom diversity,we studied three species,namely,Skeletonema costatum,Skeletonema subsalsum,and Skeletonema *** they have been found frequently in Changjiang(Yangtze)River Basin,their morphological and molecular identification is difficult in ***,to integrate morphological and molecular biological approaches,we compared systematically their morphological characters and performed phylogenetic *** strains of Skeletonema were collected and isolated from Shanghai and Jiangsu,China,and their morphological characteristics were examined by light microscopy(LM)and the scanning electron microscopy(SEM).Based on morphological comparison,we determined that *** is easy to distinguish from the other two *** heavily silicified areolae,undulated or cleft distal ends of terminal fultoportula processes(TFPPs),absence of basal pores of fultoportula processes(FPPs),the rootlike protrusions of FPPs,and no interlocking connection are the stable characteristics that can be used to identify ***,there are only two features that can distinguish *** from ***,namely the location of terminal rimoportulae(TRPs)and the distal shape of *** addition,we amplified and sequenced nine common genetic markers from the strains,from which 101 sequences were obtained,constructed phylogenetic trees based on the nine genes and evaluated that seven genes can be used to identify ***,and revealed that *** is the closest known relative of ***,and only ATP synthetase beta-subunit gene(atp B)is able to distinguish them from each other,which strongly support that it is an effective molecular marker for *** work provided a theoretical basis for the taxonomic study of Skeletonema.

关键词： Skeletonema Changjiang River morphological change distinguishing morphological characteristics molecular marker

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Development of molecular marker Linked with Cercospora Leaf Spot (CLS) Disease Resistance in Vigna radiata, Cloning, and Expression for Evaluating Antifungal Activity against Cercospora canescens

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Phyton-International Journal of Experimental Botany 2023年第4期92卷 1289-1300页

作者： Maria Babar Siddra Ijaz Imran Ul Haq Muhammad Sarwar Khan Centre of Agricultural Biochemistry and Biotechnology(CABB) University of Agriculture FaisalabadFaisalabad38040Pakistan Department of Plant Pathology University of Agriculture FaisalabadFaisalabad38040Pakistan

We developed a molecular marker for MAS of mungbean resistant varieties against CLS from the consensus sequence(MB-CLsRG)of identified RGAs(MB-ClsRCaG1 and MB-ClsRCaG2).The MB-CLsRG sequence-specific primer pair was used to screen Cercospora leaf spot(CLS)resistant varieties of mungbean in genomic analysis that showed congruency with phenotypic *** of molecular marker linkage with CLS resistance was performed using rtPCR in transcriptomic *** sequenced PCR products showed 100%homology with MB-CLsRG sequence and putative disease resistance proteins that confirmed the linkage of molecular marker with CLS resistance in *** antifungal potential of MB-CLsRG gene encoding protein was *** MB-CLsRG gene sequence was cloned in the *** expression vector for recombinant protein *** recombinant protein was then investigated for its in vitro antifungal potential against Cercospora *** in vitro investigation showed strong antifungal activity of recombinant protein as it restricted the growth of fungal mycelial *** results validated the linkage of developed marker with CLS-resistant mungbean varieties;therefore,it can be used to screen resistant varieties from a large population in ***,the recombinant protein of the MB-CLsRG gene sequence revealed antifungal potential,which proved the gene sequence could be suitable to use in transgenic plants technology to develop fungal-resistant transgenic crops.

关键词： molecular marker mungbean MAS recombinant protein antifungal potential

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Genetic Diversity of Main Inbred Indica Rice Varieties Applied in Guangdong Province as Revealed by molecular marker

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Rice science 2015年第1期22卷 1-8页

作者： HUANG Ming WU Ya-hui TAO Xing-xing LIU Yong-zhu YANG Gui-li CHEN Zhi-qiang South China Agricultural University

Genetic diversity of 299 inbred indica rice varieties, including 33 introduced varieties, applied in Guangdong Province of China were assessed using 20 ILP （intron length polymorphism） and 34 SSR （simple sequence repeat） markers. Totally, 154 loci were screened for the 299 varieties, with the average number of alleles （Na）, rare alleles （Nr）, and polymorphism information content （PIC） scored at 3.4, 0.7 and 0.32, respectively. The Nei＇s genetic distance （GD） was estimated ranging from 0 to 0.7529 with an average of 0.4797. There was no significant difference of Na, Nr, PIC or GDs between the introduced and local varieties. Neighbor-joining （N J） analysis showed that the 299 varieties failed into three main distinct groups, and the 33 introduced varieties were distributed over all the groups or subgroups. Model-based cluster analysis demonstrated that only 73 （24.4%） of the 299 varieties and 7 （21.2%） of the 33 introduced varieties could be distinctly classified into the three groups. Analysis of molecular variance showed that within the groups divided by NJ analysis, the genetic variations revealed by ILP, SSR and these two combined were 7.7%, 5.6% and 6.6%, and within the groups divided by region （Guangdong local and the introduced varieties）, the genetic variables were 2.1%, 4.6%, 5.4%, respectively. These results suggested that the genetic diversity of the 299 inbred rice varieties in Guangdong Province was low, simultaneously relationship among varieties was poor and close in all kind of groups. Hence, it is very necessary to extend the genetic diversity during the breeding and selection practical procedure.

关键词： cluster analysis genetic diversity molecular marker rice population structure

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Quantitative Evaluation of the Larval Density of Sea Potato Acaudina *** Mitochondrial molecular marker

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Journal of Ocean University of China 2021年第3期20卷 681-687页

作者： LI Xiang WANG Guilin LI Yunfan LIU Wei LU Chao GUO Yuchen HUANG Wen WANG Jinhui DONG Bo Laboratory for Morphogenesis&Evolution College of Marine Life SciencesOcean University of ChinaQingdao 266003China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China Ningde Marine Environment Monitoring Center Ningde 352100China Key Laboratory of Marine Ecological Monitoring and Restoration Technology Shanghai 200137China East China Sea Branch Ministry of Natural ResourcesShanghai 200137China

The sea potato Acaudina *** a species of sea cucumber,belonging to the class Holothuroidea under Phylum *** becomes blooming recently in the East Sea of China,causing serious ecological ***,until now there are no molecular data for its larval identification and population genetic *** this study,we firstly screened a mtDNA fragment and demonstrated that it was the species-specific molecular marker for the identification of Acaudina *** then developed a quantitative polymerase chain reaction(PCR)method to evaluate the larval density of Acaudina *** on this molecular *** this method,we examined 116 plankton samples collected in four seasons from 13 stations along the coastal region in Fu-jian province,*** results showed that the high larval density was presented at stations 1,2,and 3,which were near a quay in the *** larval density increased from April and reached the highest value in June and July,suggesting temperature might be the main environmental factor on the effects of its population distribution and *** work provides an important molecular tool for species identification and risk evaluation of a potentially invasive species.

关键词： Acaudina spp. sea potato larval density population distribution molecular marker

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molecular marker of Tumours

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Journal of Cancer Therapy 2016年第10期7卷 675-679页

作者： Marian Surma Department of Physics Adam Mickiewicz University Poznań Poland

molecular structure of the marker of tumour is determined by magneto-optical analysis of blood serum. The marker is the laevorotatory enantiomer of alanine. The cancer status of a subject is described by the number of molecules of the laevorotatory alanine enantiomer (-)ρ and the effectiveness of therapy is measured by the number of molecules of the dextrorotatory alanine enantiomer (+)ρ. The values of (-)ρ and (+)ρ are determined separately for the patient before and after therapy.

关键词： Cancer molecular marker Protein markers

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Mapping Quantitative Trait Loci Controlling Endosperm Traits with molecular marker

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Agricultural Sciences in China 2002年第1期1卷 5-10页

作者： XU Chen-wu, LI Tao, SUN Chang-sen and GU Shi-Hang( Laboratory of Quantitative Genetics , Yangzhou University, Yangzhou 225009) Laboratory of Quantitative Genetics Yangzhou University Yangzhou 225009

Based on the genetic models for triploid endosperm traits and on the methods for mapping diploid quantitative traits loci (QTLs), the genetic constitutions, components of means and genetic variances of QTL controlling endosperm traits under flanking marker genotypes of different generations were presented. From these results, a multiple linear regression method for mapping QTL underlying endosperm traits in cereals was proposed, which used the means of endosperm traits under flanking marker genotypes as a dependent variable, the coefficient of additive effect (d) and dominance effect (h1 and/or h2) of a putative QTL in a given interval as independent variables. This method can work at any position in a genome covered by markers and increase the estimation precision of QTL location and their effects by eliminating the interference of other relative QTLs. This method can also be easily used in other uneven data such as markers and quantitative traits detected or measured in plants and tissues different either in generations or at chromosomal ploidy levels, and in endosperm traits controlled by complicated genetic models considering the effects produced by genotypes of both maternal plants and seeds on them.

关键词： Endosperm traits Quantitative trait loci Multiple linear regression molecular marker

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Identification of molecular marker Linked to Salt Tolerance Gene in Alfalfa

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Agricultural Sciences in China 2005年第10期4卷 781-787页

作者： YANG Qing-chuan HAN Jian-guo SUN Yan WU Ming-sheng Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing 100094 R.P.China Institute of Grassland Science China Agricultural University Beijing 100094 R.P.China

The study has established the F2 offspring obtained by crossing salt-tolerant with salt-sensitive alfalfa, and appraised the salt-tolerant F2 offspring seedling was evaluated in pot culture. With the F2 segregated population, the research has obtained a molecular marker linked with salt-tolerant genes of alfalfa using the improved BSA combined with RAPD. The RAPD PCR products were excised from the agarose gel and purified using a kit, then were mixed with pMD-18T vector and sequenced. Sequencing result indicated the RAPD marker was 1 438 bp in length. Similarity researches using blast in Genbank indicated that the nucleotide sequence of the RAPD marker showed 93% and 91% similarity with mth2-6el8 gene fragment （347 bp） and ruth2-33122 gene fragment （334 bp） of Medicago truncatula respectively. Medicago truncatula is a close relative of alfalfa and Mth2-6el8 is a molecular marker of the gene coding for a cysteine protease which was saltinducible in some plants. These results indicated the RAPD marker was possibly related to cysteine protease genes in alfalfa.

关键词： Alfalfa Salt tolerance molecular marker Cysteine protease

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molecular marker for rapid detection of Aleurodicus disperses(Homoptera:Aleyrodidae)

Molecular marker for rapid detection of Aleurodicus disperse...

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第三届全国生物入侵大会

作者： Wei-Dong Li~*,Fu-Xiu Liu,Xiang-Shan Feng Hainan Entry-Exit Inspection and Quarantine Bureau,Haikou 570311,China

The spiralling whitefly,Aleurodicus disperses Russell,is a invasive pest worldwide. Morphological identification of the pest is very difficult due to the small size and high degree similarity with other *** this study,the method for rapid detection of *** was established based on CO I gene sequence,one pair of primers which are specific to spiralling whitefly was *** fragment amplified by the primers was 399 bp in *** tests performed with this pair of primers showed that all specimens of *** were *** this method was tested on a single male and female adult,a single nymph(1st to 4th instar),even a single egg,,and proved to be applicable for these stages of *** ***,When the dilution was 1/4000 of a whole female adult of ***,the 399 bp DNA fragment could be identified for all *** establishment of the method for rapid detection of *** provided a technical support in prevention spread of the spiralling whitefly in China and in reduction of its harmfulness.

关键词： Aleurodicus disperses molecular marker Rapid detection

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Application of molecular marker Assisted Selection in Gene Pyramiding and Selection of New Cultivars

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Journal of Northeast Agricultural University(English Edition) 2011年第1期18卷 79-84页

作者： WU Lan WANG Chao College of Horticulture Northeast Agricultural University Harbin 150000 China

The feasibility of molecule markers＇ application in gene pyramiding has been proved,and obvious progresses in crop breeding have been made till ***,different QTLs or molecular markers linked tightly to yield,quality or resistance may be used for marker assisted *** will be applied widely in crop breeding due to the development of more gene-based markers and efficient quantitative trait locus（QTL） as well as lower cost marking systems.

关键词： molecular marker gene pyramiding breeding

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Development of molecular marker Linked to Cf-10 Gene Using SSR and AFLP Method in Tomato

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Journal of Northeast Agricultural University(English Edition) 2012年第4期19卷 30-36页

作者： Li Ning Jiang Jing-bin Li Jing-fu Xu Xiang-yang Cash Crops Research Institute Hubei Academy of Agricultural Sciences Department of Horticulture College of HorticultureNortheast Agricultural University

The leaf mould resistance gene Cf-10 on tomato confered resistant or immune to all prevalent physiological races of Cladosporium fulvum presented in three northeastern provinces of China in inoculation test. In order to better utilize Cf-10 gene in a marker-assisted selection program and to permit the pyramiding of one or several resistance genes in a cultivar, tightly linked SSR and AFLP markers were obtained by the bulked segregant analysis method. One SSR marker and three AFLP markers were identified linked to Cf-10 gene, with the distance of 9.73, 5.8, 8.5, and 10.6 cM, respectively. These markers will facilitate the selection of resistant tomato germplasm containing Cf-10 gene.

关键词： tomato Cf-10 resistant gene Cladosporium fulvum molecular marker

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