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Characterization of a nitrobenzene-degrading strain Pseudomonas sp. a3 and using its immobilized cells in the treatment of mixed aromatics wastewater

Characterization of a nitrobenzene-degrading strain Pseudomo...

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第四届中国科学院博士后学术年会暨工业经济与可持续发展学术会议

作者：吴志国 Key Lab of Agricultural Environmental Microbiology Ministry of Agriculture College of Life Sciences Nanjing Agricultural University State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of Sciences

A bacterial strain Pseudomonas sp.a3 capable of degrading nitrobenzene, phenol and aniline was isolated and characterized. The appearance of aniline as one of the metabolites during the aerobic degradation of nitrobenzene indicated that a novel initial reaction existed in strain a3. To improve the degrading efficiency of strain a3, it was immobilized in the mixed carrier of polyvinyl alcohol and sodium alginate. The optimal concentration of polyvinyl alcohol and sodium alginate in the mixed carrier was 9% and 3% respectively, and the immobilized cells showed stable degradation activity and good mechanical properties. The immobilized cells also exhibited higher tolerances toward acid, high salinity environments than those of free cells. Biodegradation of nitrobenzene mixed with aniline and phenol by free and immobilized cells of Pseudomonas sp.a3 was investigated in comparison. The immobilized cells could completely degrade 300mg/L nitrobenzene within 10 hours in presence of 150 mg/L aniline and 150 mg/L phenol, which revealed that the immobilized cells of Pseudomonas sp.a3 would be a potential candidate for treating nitrobenzene wastewater mixed with other aromatics.

关键词： Pseudomonas sp.a3 Nitrobenzene Biodegradation Immobilization mixed wastewater

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Evaluation of the Purification Performance of the Kossodo wastewater Treatment Plant in Ouagadougou

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Journal of Environmental Protection 2021年第12期12卷 1128-1143页

作者： Bienvenue Joceline Sawadogo Martine Kone Rose Yamma Stéphane Nonet Philippe Walhain Roseline Kabore Toltama Sanou Théodore Nana Amado Ouedraogo H. Arsène Yonli National Centre for Scientific and Technological Research (NCSTR) Ouagadougou Burkina Faso UFR/SEA Joseph KI-ZERBO University Ouagadougou Burkina Faso Research and Expertise Center for Water (CEBEDEAU) Liege Belgium University of Liege-Start Tilman Liege Belgium National Office of Water and Sanitation (NOWS) Ouagadougou Burkina Faso

The efficiency of a wastewater treatment system is classically measured by the quality of its discharge into the aquatic environment. This quality can only be achieved if certain aspects are taken into account, such as the design and operation of the system in place. The objective of the present work is to evaluate the purification performance of this system after 17 years of operation. It should be noted that the lagoon plant (WWTP) has a total of eight basins, three of which are anaerobic (ANA). The evaluation of the physicochemical and microbiological parameters in the anaerobic basins and in the maturation basins allowed us to note slightly basic pH values in the maturation basins (8.7 to 9.2) against values from 6.8 to 7.4 in the anaerobic basins. Also, the values in terms of organic and bacteriological pollution have also evolved. For COD and BOD5, we obtained respectively abatements of more than 73% for filtered COD, 55% for gross COD and 81% for BOD5 even if the residual concentrations do not respect the standards of wastewater discharge in our country. Also, the residual concentrations of ammonia nitrogen in the output effluents (on average 12.43 mg/L), do not meet the standard of discharge into surface waters of Burkina Faso (1.29 mg/L). Thus, nutrients such as nitrogen and phosphorus pollution have average abatement rates of 31% and 27% respectively. In sum, we can say that the lagoon system set up does not meet the requirements in terms of quality of discharges, which remains an environmental problem for the receiving environment.

关键词： mixed wastewater Natural Lagooning Nitrogen Phosphorus

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