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Insect Science 2018年第1期25卷 24-32页

作者： Yasaman Alavi Anthony van Rooyen Mark Adrian Elgar Theresa Melanie Jones Andrew Raymond Weeks1 School of BioSciences University of Melbourne Victoria Australia Cesar Pty Ltd 293 Royal Parade Parkville Victoria Australia

Parthenogenetic reproduction is taxonomically widespread and occurs through various cytological mechanisms, which have different impact on the genetic variation of the offspring. Extatosoma tiaratum is a facultatively parthenogenetic Australian insect （Phasmatodea）, in which females oviposit continuously throughout their adult lifespan irre- spective of mating. Fertilized eggs produce sons and daughters through sexual reproduction and unfertilized eggs produce female offspring via parthenogenesis. Here, we developed novel microsatellite markers for E. tiaratum and characterized them by genotyping indi- viduals from a natural population. We then used the microsatellite markers to infer the cytological mechanism of parthenogenesis in this species. We found evidence suggesting parthenogenesis in E. tiaratum occurs through automixis with terminal fusion, resulting in substantial loss of microsatellite heterozygosity in the offspring. Loss of microsatel- lite heterozygosity may be associated with loss of heterozygosity in fitness related loci. The mechanism of parthenogenetic reproduction can therefore affect fitness outcomes and needs to be considered when comparing costs and benefits of sex versus parthenogenesis.

关键词： apomixis automixis Extatosoma tiaratum heterozygosity microsatellite markers parthenogenesis

Development and Characterization of microsatellite markers for Harpadon nehereus Based on High-Throughput Sequencing and Cross-Species Amplification in Three Myctophiformes Fishes

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Journal of Ocean University of China 2023年第1期22卷 181-188页

作者： HUANG Xinxin NING Zijun YANG Tianyan Fisheries College Zhejiang Ocean UniversityZhoushan 316022China

Harpadon nehereus is a widespread economical fish found in the coastal seas of China and has important ecological value in the marine ecosystem.H_(o)wever,its germplasm resources have been seriously degraded due to natural factors and anthropogenic *** this study,high-throughput sequencing was applied to search for microsatellite loci in *** transcriptome to provide references for its resource conservation and *** loci were developed by non-denaturing polyacrylamide gel electrophoresis,and their cross-species amplified ability was detected in three related species.A total of 5652 microsatellites were identified from 16974320 *** the primer pairs designed for 100 SSRs for PCR amplification,80%were successfully amplified,and 26 loci were polymorphic with a high number of alleles from 3 to 11 *** expected(H_(e))and observed(H_(o))heterozygosities were 0.355–0.885 and 0.375–0.958,*** of the loci were highly polymorphic(polymorphism information content:0.316–0.852;mean:0.713),and these markers can be applied in the population genetic diversity research of ***.H_(o)wever,the transferability of these primers was low,probably because of the close relation of the collected *** follow-up work,simple sequence repeats will be excavated with genome-based technologies,and related species will be gathered to address the present inadequacies.

关键词： Harpadon nehereus microsatellite markers high-throughput sequencing cross-species amplification

Development and characterization of novel polymorphic microsatellite markers for the Korean freshwater snail Semisulcospira coreana and cross-species amplification using next-generation sequencing

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Journal of Oceanology and Limnology 2020年第2期38卷 503-508页

作者： Yeon Jung PARK Mi Nan LEE Eun-Mi KIM Jung Youn PARK Jae Koo NOH Tae-Jin CHOI Jung-Ha KANG Biotechnology Research Division National Institute of Fisheries ScienceBusan 46083Republic of Korea Department of Microbiology Pukyoung National UniversityBusan 48513Republic of Korea

Korean freshwater snails of the genus Semisulcospira are widely distributed across East *** has been a very popular nutritional food in Korea,and is an ecologically important water quality indicator because it lives only in clean ***,no microsatellite markers have been generated to study the population genetic diversity of this *** the present study,we developed and characterized 18 novel microsatellite loci from Semisulcospira coreana genomic *** microsatellites were isolated using 454 GS-FLX titanium sequencing and 18 markers were used for genotyping in *** addition,we also tested the cross-species transferability of the microsatellite markers in four additional Semisulcospira *** identified 18 polymorphic loci and the number of alleles per loci,and their polymorphism information content values ranged from 2 to 17 and 0.203 to 0.902,*** observed and expected heterozygosities of the loci ranged from 0.063 to 0.924 and 0.226 to 0.924,*** to the analysis of the cross-species transferability of these markers,four species,***,***,***,and ***,showed a very high transferability(80%–85%).These results show that this set of nuclear markers could be useful for population genetics studies of this species and closely related species.

关键词： Semisulcospira next-generation sequencing(NGS) microsatellite markers cross-species transferability

Discordant patterns of genetic variation between mitochondrial and microsatellite markers in Acanthogobius ommaturus across the coastal areas of China

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Acta Oceanologica Sinica 2023年第4期42卷 72-80页

作者： Chenyu Song Zhong Tu Na Song Key Laboratory of Mariculture(Ocean University of China) Ministry of EducationQingdao 266003China Shandong Fisheries Development and Resources Conservation Center Yantai 264003China

Acanthogobius ommaturus, which belongs to the family Gobiidae, is a euryhaline and demersal fish that is widely distributed in the coastal areas, harbors, and estuaries of China, D. P. R. Korea and Japan. In this study, the genetic diversity and genetic structure of five geographical populations of A. ommaturus was assessed using the mitochondrial hypervariable region gene and microsatellite markers. The results of the two genetic markers indicated that the A. ommaturus populations had a high level of genetic diversity. The mitochondrial marker detected weak genetic differentiation among populations, and the Neighbor-Joining tree showed that there was no obvious pedigree branches and geographic structure as well. However, population of Zhoushan showed significant genetic differentiation with other populations by microsatellite markers. The population of *** has not experienced bottleneck effect recently. We speculated that the Pleistocene climate change and juvenile fish dispersal played an important role in the population differentiation of A. ommaturus.

关键词： mitochondrial markers microsatellite markers genetic diversity genetic structure Acanthogobius ommaturus

Screening microsatellite markers from EST sequences of bay scallop Argopecten irradians

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High Technology Letters 2006年第1期12卷 97-102页

作者：王玲玲 Song Linsheng Xu Wei Zhang Huan Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 P.R. China Graduate School Chinese Academy of Sciences Beijing 100039 P.R. China

Seventy-five simple sequence repeats （SSRs） were identified by the bioinformatic analysis from 5008 expressed sequence tags （ESTs） of Argopecten irradians. Among the SSRs, the number of repeat nucleotide varied from 2 to 6. Dinucleotide and trinueleotide repeat motifs were dominant in EST-SSRs of bay scallop, with a proportion of 80% over the total screened SSRs. Twenty-nine pairs of primer were designed based on the flank sequences of the selected ESTs using the software of Primet 5, and verified under the given PCR reaction condition. Eighteen of the 29 primer pairs resulted in the expected products, while the remaining either failed to produce any fragments or yielded products over expected size. Thirteen of the 18 SSRs, accounting for 72%, were detected to show polymorphism in the examined scallop samples. A preliminary test in this study indicated that the majority of the identified SSRs were informative in the cultured bay scallops, making them suitable for the population and other genetic analysis. EST-SSR markers have more advantages than the traditional genomic-derived SSRs and there is a wide range of application in comparative mapping, functional gene cloning and marker assisted selection. This research provides a reference to the identification of EST-SSRs with relative bioinformatic analysis from aquaculture species, as well as to those with a large number of ESTs.

关键词： Argopecten irradians microsatellite markers expressed sequence tags

Mapping QTLs Affecting Economic Traits on BTA3 in Chinese Holstein with microsatellite markers

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Journal of Integrative Agriculture 2014年第9期13卷 1999-2004页

作者： QIN Chun-hua CHU Qin CHU Gui-yan ZHANG Yi ZHANG Qin ZHANG Sheng-li SUN Dong-xiao College of Animal Science and Technology/Key Laboratory of Animal Genetics and Breeding Ministry of Agriculture/National Engineering Laboratory of Animal Genetics China Agricultural University

It had been demonstrated that the strong and highly significant quantitative trait locus（QTL） can affect protein percentage on Bos Taurus Autosome 3（BTA3） at the position 52 cM, near the microsatellite DIK4353, with the 95% confidence interval spanning from 25 to 57 cM in Chinese Holstein population using QTL-express, MQREML, and GRIDQTL softwares. This study herein focused on such region of fine mapping QTLs for milk production and functional traits with 16 microsatellite markers with coverage of 33 cM between the markers BMS2904 and MB099 on BTA3 in a daughter-designed Chinese Holstein population. A total of 1 298 Holstein cows and 7 sires were genotyped for 16 microsatellites with ABI 3700 DNA sequencer. The variance components QTL linkage analysis（LA） and linkage-disequilibrium（LD） analysis（LA/LD） was performed to map QTLs for 7 traits, i.e., 305-d milk yield, fat yield, protein yield, fat percentage, protein percentage, somatic cell score and persistency of milk yield. Four strong and highly significant QTLs were detected for fat yield, fat percentage, protein percentage and somatic cell score at the position 40, 30, 27 and 26 cM, respectively. Two minor QTLs for milk yield and persistency of milk yield were identified at 42 and 46 cM, respectively. These findings provided a general idea for the fine mapping of the causal mutation for milk production and functional traits on BTA3 in the future.

关键词： QTL mapping economic traits microsatellite markers BTA3 Chinese Holstein

Morphometric analysis and fluorescent microsatellite markers to evaluate the genetic diversity of five populations of Penaeus japonicus in China

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Aquaculture and Fisheries 2022年第3期7卷 321-327页

作者： Jinghao Du Chunxiu Hou Xiuli Chen Jie Xiao Yasmeen Gul Huanling Wang Key Lab of Freshwater Animal Breeding Key Laboratory of Agricultural Animal GeneticsBreeding and ReproductionMinistry of EducationCollege of FisheryHuazhong Agricultural UniversityWuhan430070China Guangxi Academy of Fishery Sciences Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy BreedingNanning530021China Department of Zoology Government College Women University FaisalabadFaisalabad38000Pakistan

To understand the current status of germplasm resources of Penaeus japonicus,in this study,genetic diversity was analyzed based on morphometric analysis and fluorescent microsatellite *** morphometric analysis,the cumulative ratios of the PC1 and PC2(85.90%)were greater than 75%,which can explain the morphological variation among *** microsatellite analysis,the allele number in 10 microsatellite loci ranged from 27 to 53,and the effective allele number ranged from 21 to *** observed heterozygosity ranged from 0.463 to 0.983,whereas expected heterozygosity ranged from 0.932 to 0.966,and the average polymorphism information content was 0.947,which indicated a high level of genetic *** unbiased genetic distance among populations ranged from 0.230 to 0.431,and the genetic similarity coefficient ranged from 0.654 to *** of molecular variance(AMOVA)showed that the genetic variation within individuals was the main source of variation,accounting for 88.55%,and only 1.49%of the genetic variation came from among populations.

关键词： Penaeus japonicus Morphometric analysis microsatellite markers Genetic diversity Genetic structure

microsatellite markers for main fresh water aquaculture species in China

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Microsatellite markers for main fresh water aquaculture spec...

Identification of seeds of Pinus species by microsatellite markers

2005水产科技论坛

作者：孙效文 MARC Welt Heilongjiang River Fisheries Research Institute CAFS and Key Lab. of Bioengineering Breeding of Northern Finfish-Ministry of Agriculture the Peoples-Republic of China. Harbin 150070 China College of Pharmacy Division of Basic Pharmaceutical SicenceXavier University of LouisianaUSA

＜正＞The microsatellite DNA from seven main fresh water aquatic species including common carp, silver crucian carp, grass carp, silver carp, bighead carp, snout bream, Chinese river crab was cloned by two methods. One method is screening short inserted DNA library using a few synthetic oligonucleotide simple repeated sequence labeled at the 5’end with [γ -p32] ATP. Other method is based on using in biotinylated probes attached to magnetic beads to isolates pieces of DNA containing the microsatellites. We got 1 180 microsatellite DNA for common carp, 137 for silver crucian carp, 114 for grass carp, 138 for silver carp, 75 for bighead carp, 108 for Chinese river crab, 120 for snout bream. PCR primers were designed using software PRIMER5. A total of 210 primer sets were test for polymorphism on each species panels of six animals. 60% markers were found to be polymorphic. These polymorphic markers as well as non-polymorphic markers will be very valuable as genetic tools for using in strain identification, parentage analysis, quantity trait loci analysis, population structure analysis and marker-based selection studies.

关键词： microsatellite markers aquaculture species

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Journal of Forestry Research 2005年第4期16卷 281-284页

作者： FU Xiang-xiang SHI Ji-sen Nanjing Forestry University Nanjing 210037 P. R. China

The 276 pair-primers （nuclear and chloroplast microsatellite） developed from seven species of Pinaceae were selected and identified for cross-species transferability to ten Pinus species （P massoniana, P kesiya, P tabulaeformis P densiflora, P thunbergii, P caribaea, P taeda, P yunnanensis, P densata, P sylvestris）belonging to Sect. Pinus by BSA （bulked segregate analysis） method. The results showed that 23 of 276 （8.0%） markers were successful to have amplification product in ten species, and 5 of 23 （21.7%） were polymorphic cross species and lack of polymorphic within species. Eight of 10 Pinus species were identified by using single primer, two and more combination of primers, but there were still no effective SSR primers for identifying other 2 species （P. kesiya and P. densata）.

关键词： Identification Simple sequence repeats (SSRs) Pinus species microsatellite markers

Cross-species microsatellite markers for revealing population genetic structure of the woolly apple aphid Eriosoma lanigerum

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Cross-species microsatellite markers for revealing populatio...