Identification of seeds of Pinus species by microsatellite markers

作     者：FU Xiang-xiang SHI Ji-sen FU Xiang-xiang;SHI Ji-sen

作者机构：Nanjing Forestry University Nanjing 210037 P. R. China 

出 版 物：《Journal of Forestry Research》 (林业研究（英文版）)

年 卷 期：2005年第16卷第4期

页      面：281-284页

核心收录：

学科分类：0907[农学-林学] 08[工学] 0829[工学-林业工程] 09[农学] 

主　　题：Identification Simple sequence repeats (SSRs) Pinus species Microsatellite Markers 

摘      要：The 276 pair-primers （nuclear and chloroplast microsatellite） developed from seven species of Pinaceae were selected and identified for cross-species transferability to ten Pinus species （P massoniana, P kesiya, P tabulaeformis P densiflora, P thunbergii, P caribaea, P taeda, P yunnanensis, P densata, P sylvestris）belonging to Sect. Pinus by BSA （bulked segregate analysis） method. The results showed that 23 of 276 （8.0%） markers were successful to have amplification product in ten species, and 5 of 23 （21.7%） were polymorphic cross species and lack of polymorphic within species. Eight of 10 Pinus species were identified by using single primer, two and more combination of primers, but there were still no effective SSR primers for identifying other 2 species （P. kesiya and P. densata）.