检索结果-南通市图书馆

Bioactive Materials 2022年第8期7卷 134-144页

作者： Xin Qin Lirong Xiao Ni Li Chen Hou Wenman Li Jiajie Li Naihong Yan Yunfeng Lin State Key Laboratory of Oral Diseases National Clinical Research Center for Oral DiseasesWest China Hospital of StomatologySichuan UniversityChengdu610041China Research Laboratory of Ophthalmology West China HospitalSichuan UniversityChengdu610041China College of Biomedical Engineering Sichuan UniversityChengdu610041China

Choroidal neovascularization(CNV)is a common pathological feature of various eye diseases and an important cause of visual impairment in middle-aged and elderly patients.In previous studies,tetrahedral framework nucleic acids(tFNAs)showed good carrier performance.In this experiment,we developed MicroRNA-155-equipped tFNAs(T-155)and explored its biological effects on CNV.Based on the results of in-vitro experiments,T-155 could regulate macrophages into the antiangiogenic M1 type.Then,we injected T-155 into the vitreous of laser-induced CNV model mice and found that T-155 significantly reduced the size and area of CNV,inhibited blood vessel leakage.In summary,we prove that T-155 could regulate the inflammatory process of CNV by polarizing macrophages,thereby improving the symptoms of CNV.Thus,T-155 might become a new DNA-based drug with great potential for treating CNV.

关键词： Choroidal neovascularization Tetrahedral framework nucleic acid Macrophages MicroRNA-155 Immunity therapy

MicroRNA-155靶向SOCS1基因对冠心病内皮细胞炎症损伤的影响

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国老年学杂志 2022年第23期42卷 5858-5863页

作者：徐涛王超河北北方学院附属第一医院心血管内科河北张家石075000 河北省人民医院代谢病重点实验室

目的探讨MicroRNA-155靶向细胞因子信号转导抑制因子(SOCS)1基因对冠状动脉粥样硬化性心脏病(CHD)内皮细胞炎症损伤的作用。方法构建动脉粥样硬化(AS)大鼠模型,分离、培养、鉴定大鼠原代冠状动脉内皮细胞,使用同型半胱氨酸与细胞共孵育2... 详细信息

目的探讨MicroRNA-155靶向细胞因子信号转导抑制因子(SOCS)1基因对冠状动脉粥样硬化性心脏病(CHD)内皮细胞炎症损伤的作用。方法构建动脉粥样硬化(AS)大鼠模型,分离、培养、鉴定大鼠原代冠状动脉内皮细胞,使用同型半胱氨酸与细胞共孵育24 h,进行冠心病应激,将细胞分组。双荧光素酶报告基因实验验证MicroRNA-155和SOCS1的靶向关系。实时定量反转录聚合酶链反应(qRT-PCR)和Western印迹检测心肌组织和各组细胞中MicroRNA-155和SOCS1表达水平。四甲基偶氮唑(MTT)法检测细胞活力。使用Hochest33258染色检验细胞凋亡率。酶联免疫吸附试验(ELISA)检测白细胞介素(IL)-1β、IL-6、IL-10和IL-18表达。结果与正常组相比,AS组血清中三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、Ca^(2+)水平均明显提高(PMicroRNA-155水平显著升高,SOCS1 mRNA及蛋白水平显著下降(PMicroRNA-155的靶基因。与空白对照组相比,MicroRNA-155mimics组SOCS1蛋白水平下降,细胞活力降低,细胞凋亡率升高,IL-1β、IL-6和IL-18表达水平上升,IL-10表达水平下降,差异均有统计学意义(PMicroRNA-155 inhibitor组的SOCS1 mRNA及蛋白水平上调,细胞活力增加,细胞凋亡率下降,IL-1β、IL-6和IL-18表达水平下降,IL-10表达水平上升,差异均有统计学意义(PMicroRNA-155通过靶向抑制SOCS1基因,进而增加冠心病大鼠心肌微血管内皮细胞凋亡率及增加促炎症因子的表达,从而发挥对心肌微血管内皮细胞的促炎作用。

关键词：冠心病 MicroRNA-155 细胞因子信号转导抑制因子(SOCS)1 炎症因子

MicroRNA-155在缺氧肺癌脑转移中的作用

在线全文

学校读者我要写书评

暂无评论

四川大学学报（医学版） 2019年第6期50卷 835-839页

作者：刘洋贾永森秦丽娟华北理工大学基础医学院唐山063210 华北理工大学中医学院唐山063210 河北省慢性疾病重点实验室唐山063210

目的明确缺氧与肺癌细胞释放MicroRNA-155的关系,揭示肺癌脑转移的可能机制。方法建立人A549肺癌细胞缺氧模型,缺氧培养肺癌细胞0.5、2、4、8、12和24 h(并设同期常氧对照组)后,免疫荧光法和Western blot法测定肺癌细胞内热休克蛋白70 (... 详细信息

目的明确缺氧与肺癌细胞释放MicroRNA-155的关系,揭示肺癌脑转移的可能机制。方法建立人A549肺癌细胞缺氧模型,缺氧培养肺癌细胞0.5、2、4、8、12和24 h(并设同期常氧对照组)后,免疫荧光法和Western blot法测定肺癌细胞内热休克蛋白70 (hsp70)的表达水平;建立hsp70过表达的人肺癌细胞株A549/hsp70,实时荧光定量PCR(qRT-PCR)法测定缺氧各时点A549细胞和A549/hsp70细胞培养液内MicroRNA-155含量。构建体外血脑屏障模型,给予缺氧不同时间点的A549肺癌细胞培养液后,辣根过氧化物酶(horseradish peroxidase, HRP)检测体外血脑屏障模型的通透性变化;自动细胞计数仪计数Transwell下室培养液中的A549肺癌细胞数。将缺氧不同时间点的A549肺癌细胞培养液经尾静脉注入Wistar大鼠体内,Western blot法测定紧密连接蛋白occludin在脑组织血脑屏障内皮细胞中的表达水平;伊文思兰检测动物血脑屏障通透性改变。结果肺癌细胞缺氧8 h时,肺癌细胞内hsp70和培养液内MicroRNA-155的表达均达最高水平(PMicroRNA-155含量的增加更显著。缺氧8 h,血脑屏障通透性最大,透过血脑屏障模型的肺癌细胞数最多。在动物水平,给予缺氧8 h的肺癌细胞培养液后,动物脑组织血脑屏障内皮细胞的occludin表达最低,且此时实验动物的血脑屏障通透性亦最大。结论缺氧可引起肺癌细胞内hsp70生成增多,促进了MicroRNA-155的表达,进而导致血脑屏障occludin表达减少,血脑屏障通透性增大,最终引起肺癌细胞转移入脑。

关键词：肺癌脑转移 hsp70 MicroRNA-155 紧密连接蛋白

MicroRNA-155 promotes the pathogenesis of experimental colitis by repressing SHIP-1 expression

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2017年第6期23卷 976-985页

作者： Zhan-Jun Lu Jian-Jiong Wu Wei-Liang Jiang Jun-Hua Xiao Kai-Zhong Tao Lei Ma Ping Zheng Rong Wan Xing-Peng Wang Department of Gastroenterology Shanghai General HospitalShanghai Jiao Tong University School of Medicine Department of Gastroenterology Shanghai East HospitalSchool of MedicineTong Ji University

AIM To explore the mechanism by which micro RNA-155(miR-155) regulates the pathogenesis of experimental colitis.METHODS A luciferase assay was performed to confirm the binding of miR-155 to the SHIP-1 3'-UTR. MiR-155 mimics, negative controls and SHIP-1 expression/knockdown vectors were established and then utilized in gain-and loss-of-function studies performed in raw264.7 cells and primary bone marrow-derived macrophages (BMDMs). Thereafter, dextran sulfate sodium(DSS)-induced colitis mouse model with or without antagomiR-155 treatment was established, and the levels of miR-155 and SHIP-1, as well as the pro-inflammatory capabilities, were measured by western blot, quantitative polymerase chain reaction, and immunohistochemistry.RESULTS MiR-155 directly bound to the 3'-UTR of SHIP-1 mRNA and induced a significant decrease in SHIP-1 expression in both raw264.7 cells and primary BMDMs. MiR-155 markedly promoted cell proliferation and proinflammatory secretions including IL-6, TNF-α, IL-1β, and IFN-γ, whereas these effects could be reversed by the restoration of SHIP-1 expression. In vivo studies showed that antagomiR-155 administration could alleviate DSS-induced intestinal inflammation in Balb/c mice. Moreover, significantly increased SHIP-1 expression, as well as decreased Akt activation and inflammatory response, were observed in the antagomiR-155-treated mice.CONCLUSION MiR-155 promotes experimental colitis by repressing SHIP-1 expression. Thus, the inhibition of miR-155 might be a promising strategy for therapy.

关键词：试验性的大肠炎煽动性的肠疾病 MicroRNA-155 SHIP-1

Association between the level of CD4^+T lymphocyte MicroRNA-155 and coronary artery disease in patients with unstable angina pectoris

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Geriatric Cardiology 2018年第10期15卷 611-617页

作者： Zi-Liang WE Hai-Li LU Qiang SU Lang Li Department of Cardiology the First Affiliated Hospital of Guangxi Medical UniversityGuangxi Cardiovascular InstituteNanningGuangxiChina Department of Orthodontics the Affiliated Dental Hospitdl of Guangxi Medical UniversityNanningCruangxiChina

Objective To study the association between the expression of MicroRNA-155(miRNA-155)in peripheral blood CD4^+T lymphocytes and the level of semrn interferon-7(IFN-7)concentration and the severity of coronary artery disease (CAD).Methods After coronary angiography,252patients with suspected unstable angina pectoris (UAP)were divided into the UAP group (128patients with CAD confirmed by angiography)and the control group (124patients without CAD confirmed by angiography).Fresh peripheral blood was extracted 16-24h before coronary angiography,CD4^+T lymphocytes was tested using immunomagnetic beads,the expression ofmiRNA-155was tested using quantitative PCR and the expression of IFN-7was tested using enzyme-linked immunosorbent assay (ELISA).According to the results of angiography,Gensini score of coronary artery lesions was analyzed.Furthermore,we also analysis the association between the level of miRNA-155in peripheral blood CD4^+T lymphocytes,the level of serum IFN-γand Gensini score of coronary lesion.Results The levels ofmiRNA-155(0.49±0.08vs.0.23±0.09)and IFN-7(227.58±26.01vs.141.23±17.89)in the UAP group were significantly higher than that of the control group,the difference was statistically significant.The level of miRNA-155and IFN-γwere positively correlated with Gensini score of CAD (r =0.534,r =0.713,respectively,all P 155was positively correlated with the level of IFN-γ,(r =0.686,P 155in peripheral blood CD4^+T lymphocytes and the level of IFN-γ are closely correlated with the severity of CAD.

关键词： CD4^+T lymphocyte Coronary artery disease IFN-γ MicroRNA-155

血清MicroRNA-155与尿酸在早发急性心肌梗死中的研究分析

在线全文

学校读者我要写书评

暂无评论

血清microRNA-155与尿酸在早发急性心肌梗死中的研究分析

作者：唐智江昆明医科大学

学位级别：硕士

[目的]本研究探讨血清MicroRNA-155在早发急性心肌梗死患者中的表达水平及作用,分析血清尿酸在早发急性心肌梗死患者中的水平差异,并分析血清MicroRNA-155和尿酸的相关性,进而探究尿酸能否通过影响MicroRNA-155的表达而影响早发急性心... 详细信息

[目的]本研究探讨血清MicroRNA-155在早发急性心肌梗死患者中的表达水平及作用,分析血清尿酸在早发急性心肌梗死患者中的水平差异,并分析血清MicroRNA-155和尿酸的相关性,进而探究尿酸能否通过影响MicroRNA-155的表达而影响早发急性心肌梗死的发生、发展,以期为早发急性心肌梗死的防治提供理论依据。[方法]研究选取2021年01月至2022年01月于昆明医科大学附属曲靖医院心内科住院治疗并知情同意行冠状动脉造影的新发的早发急性心肌梗死患者62例（男性＜55岁,女性＜65岁）为病例组,其中急性非ST段抬高型心肌梗死患者32例、ST段抬高型心肌梗死患者30例,以同期住院行冠状动脉造影或冠脉CTA排外冠状动脉粥样硬化或冠心病的同龄患者62例为对照组。入院后在取得患者知情同意基础上,详细记录患者的一般临床资料:年龄、性别、身高、体重、高血压史、糖尿病史、吸烟史、饮酒史、血压、心率;检验及检查指标:血脂、血糖、肝功、肌酐、尿酸、同型半胱氨酸、冠状动脉CTA/冠脉造影结果,同时抽取患者入院当天或次日清晨的外周静脉血分离出血清,于昆明医科大学附属曲靖医院生物实验室采用RT-PCR法检测患者血清中MicroRNA-155表达量。采用Gensini评分评价病例组患者的冠脉病变严重程度。[结果]1.早发急性心肌梗死组合并吸烟与糖尿病的患者比例及胆固醇、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇、空腹血糖水平均高于对照组（P均＜0.05）,而血清尿酸、性别、年龄、高血压病史、BMI、入院血压及心率、甘油三脂、载脂蛋白A1、载脂蛋白B、脂蛋白a、血清肌酐、HCY、WBC、ALT两组间差异无统计学意义（P均＞0.05）,且血清尿酸在早发NSTEMI与早发STEMI组间差异亦无统计学意义（P＞0.05）。2.早发急性心肌梗死组外周血血清MicroRNA-155表达量显著低于对照组（P＜0.05）,而在早发NSTEMI组与早发STEMI组间MicroRNA-155表达量差异无统计学意义（P＞0.05）;3.无论是在早发急性心肌梗死组还是对照组,高尿酸组与正常尿酸组间MicroRNA-155表达量差异均无统计学意义（P均＞0.05）;4.对照组、早发NSTEMI组、早发STEMI组两两比较结果显示:吸烟史、糖尿病史、胆固醇、低密度脂蛋白胆固醇、空腹血糖、MicroRNA-155表达量在早发NSTEMI组与早发STEMI组间差异均无统计学意义（P均＞0.05）。早发NSTEMI组与早发STEMI组空腹血糖水平、吸烟史比例均高于对照组（P均＜0.05）、MicroRNA-155表达量均低于对照组（P＜0.05）;早发NSTEMI组胆固醇、低密度脂蛋白胆固醇高于对照组（P均＜0.05）,而在早发STEMI组与对照组间胆固醇、低密度脂蛋白胆固醇差异却无统计学意义（P均＞0.05）;糖尿病史在早发STEMI组与对照组间差异有统计学意义（P＜0.05）,在早发NSTEMI组与对照组间差异无统计学意义（P均＞0.05）5.在所有研究对象中MicroRNA-155表达量与空腹血糖、胆固醇、高密度脂蛋白胆固醇均呈负相关（空腹血糖r=-0.135,P=0.000;胆固醇r=-0.188,P=0.037;高密度脂蛋白胆固醇r=-0.220,P=0.014）,但分别在早发急性心肌梗死组与对照组间行相关性检验结果却无统计学意义（P均＞0.05）。在早发急性心肌梗死组中 MicroRNA-155 表达量与 Gensini 评分呈负相关（r=-0.286,P=0.031）。6.Logistics回归分析结果显示,与对照组相比,空腹血糖升高、具有吸烟史、MicroRNA-155表达量下降是早发急性心肌梗死的危险因素（P均＜0.05）,其中吸烟史的影响最大;早发NSTEMI组与对照组相比,低密度脂蛋白胆固醇也是早发NSTEMI的危险因素（P＜0.05）。[结论]1.早发急性心肌梗死患者血清MicroRNA-155表达显著降低,在NSTEMI与STEMI间表达无差异。2.早发急性心肌梗死组中MicroRNA-155表达量与Gensini评分呈负相关,而在所有研究对象中microrna表达量与胆固醇、空腹血糖水平呈负相关,MicroRNA-155可能对早发急性心肌梗死的发生、发展起着保护作用。3.血清尿酸水平在早发急性心肌梗死与对照组间无统计学差异,考虑与样本量小有关,本研究尚未发现血清尿酸与MicroRNA-155有相关性,未来需要大样本研究进一步明确二者关系。

关键词：早发急性心肌梗死 MicroRNA-155 尿酸急性非ST段抬高型心肌梗死急性ST段抬高型心肌梗死

MicroRNA-155对自噬的调节在胃癌诊治中的作用研究进展

同方学位论文库评论

在线全文

同方学位论文库

学校读者我要写书评

暂无评论

中南大学学报（医学版） 2019年第1期44卷 87-91页

作者：陈刚张敏敏李玉民甘肃省消化系肿瘤重点实验室兰州730030 兰州大学第二医院普外一科兰州730030 甘肃中医药大学临床医学院兰州730000

我国是全球胃癌高发地区之一,早期检出率低,疗效差,5年存活率低,手术联合放射治疗和化学药物治疗是主流的综合治疗方案,但化学药物治疗多药耐药普遍存在。化学药物治疗、miRNA和自噬三者之间相互影响,但机制复杂。MiR-155在胃癌中表达异... 详细信息

我国是全球胃癌高发地区之一,早期检出率低,疗效差,5年存活率低,手术联合放射治疗和化学药物治疗是主流的综合治疗方案,但化学药物治疗多药耐药普遍存在。化学药物治疗、miRNA和自噬三者之间相互影响,但机制复杂。MiR-155在胃癌中表达异常,促进自噬,抑制凋亡,与幽门螺杆菌相互作用诱发胃癌。通过miR-155调节自噬可以用来治疗胃癌,还可用于改善化学药物治疗敏感性以逆转耐药。MiR-155在胃癌组织中的高表达提示预后不良。

关键词：胃癌 MicroRNA-155 自噬多药耐药

MicroRNA-155在甲状腺乳头状癌中的表达及临床意义

在线全文

学校读者我要写书评

暂无评论

南方医科大学学报 2016年第10期36卷 1364-1368页

作者：朱有志郑珂张惠灏陈玲吴坤琳任彩虹王宗财孔令君阮炜鸿陈祥锦福建医科大学附属第一医院甲状腺乳腺外科福建福州350000 福建医科大学附属第一医院病理科福建福州350000

目的探讨MicroRNA-155(miR-155)在甲状腺乳头状癌(papillary thyroid cancer,PTC)患者中的表达情况及与临床病理特征的相关性,miR-155指导PTC预后的可行性。方法收集86例PTC患者的癌及癌旁新鲜组织,用荧光定量PCR的方法检测miR-155在上... 详细信息

目的探讨MicroRNA-155(miR-155)在甲状腺乳头状癌(papillary thyroid cancer,PTC)患者中的表达情况及与临床病理特征的相关性,miR-155指导PTC预后的可行性。方法收集86例PTC患者的癌及癌旁新鲜组织,用荧光定量PCR的方法检测miR-155在上述组织中的表达,并结合其临床病理特点进行回顾性分析。结果 69.8%(60/86)的PTC患者癌组织的miR-155表达较癌旁正常组织上调,相对上调倍数为2.63±2.73倍;而在表达上调组中,PTC原发灶更大[(1.66±0.96)cm vs(1.19±0.52)cm,P=0.021];更容易出现甲状腺包膜外侵犯(56.7%vs 23.1%,P=0.004);淋巴结转移率更高(70%vs 46.2%,P=0.036);具有更晚的TNM分期,Ⅲ~Ⅳ期的比例更高(20%vs 0%,P=0.014)。PTC癌组织中miR-155相对表达量(ΔCT)高低与淋巴结转移个数具备一定的相关性(r=0.531,P=0.001)。结论 PTC中miR-155的高表达具有更大肿瘤病灶,更易甲状腺包膜外侵犯,更高颈部淋巴结转移率及更晚的TNM分期等临床病理特点,可能作为不良临床预后的判断指标之一。

关键词：甲状腺肿瘤甲状腺乳头状癌 MicroRNA-155

The altered expression of inflammation-related micrornas with MicroRNA-155 expression correlates with Th17 differentiation in patients with acute coronary syndrome

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2011年第6期8卷 486-495页

作者： Rui Yao Yulan Ma Youyou Du Mengyang Liao Huanhuan Li Wei Liang Jing Yuan Zhijun Ma Xian Yu Hong Xiao Yuhua Liao Laboratory of Cardiovascular Immunology Institute of CardiologyUnion HospitalHuazhong University of Science and TechnologyWuhanChina Department of Cardiology the First Affiliated Hospital of Zhengzhou UniversityZhengzhouChina Department of General Surgery Union HospitalHuazhong University of Science and TechnologyWuhanChina Pharmaceutical Preparation Section the First Hospital of Wuhan CityWuhanChina

micrornas(miRNAs)are a novel class of small,non-coding RNAs that play a significant role in both inflammatory and cardiovascular diseases.Immune cells,especially T helper(Th)cells,are critical in the development of atherosclerosis and the onset of acute coronary syndrome(ACS).To assess whether inflammation-related miRNAs(such as miR-155,146a,21,125a-5p,125b,31)are involved in the imbalance of Th cell subsets in patients with ACS,we measured the expression of related miRNAs in patients with acute myocardial infarction(AMI),unstable angina(UA),stable angina(SA)and chest pain syndrome(CPS);analyzed the relationship between miRNA expression and the frequency of Th cell subsets;and observed the co-expression of miR-155 and IL-17A in peripheral blood mononuclear cells(PBMCs)of patients with ACS.The results showed that the expression of miR-155 in the PBMCs of patients with ACS was decreased by approximately 60%,while the expression of both miR-21 and miR-146a was increased by approximately twofold.The expression patterns of miRNAs in plasma correlated with those in PBMCs,except for miR-21,which was increased by approximately sixfold in the AMI group and showed no significant difference between the UA group and the CPS group.We also found that the expression of miR-155 inversely correlated with the frequency of Th17 cells(r520.896,P,0.01)and that miR-155 was co-expressed with IL-17A in patients with ACS.In conclusion,our study revealed the expression patterns of inflammation-related miRNAs in patients with ACS and found that miR-155 may be associated with Th17 cell differentiation.

关键词： acute coronary syndrome MicroRNA-155 Th17

MicroRNA-155在原发性开角型青光眼发病机制中的作用

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

国际眼科杂志 2018年第5期18卷 836-839页

作者：杨茹菲苏颖蒋鑫王峰哈尔滨医科大学附属第一医院眼科分院中国黑龙江省哈尔滨市150001

microrna在眼部组织有特定表达,对眼部组织发挥独特作用。MicroRNA-155可调控多种信号通路,影响相应蛋白的表达,导致细胞、组织的形态和功能等异常。原发性开角型青光眼的产生机制尚不完全明确,现认为小梁网的形态功能异常是引起眼内压... 详细信息

microrna在眼部组织有特定表达,对眼部组织发挥独特作用。MicroRNA-155可调控多种信号通路,影响相应蛋白的表达,导致细胞、组织的形态和功能等异常。原发性开角型青光眼的产生机制尚不完全明确,现认为小梁网的形态功能异常是引起眼内压升高的主要原因。研究MicroRNA-155对原发性开角型青光眼发生发展的影响,可以为原发性开角型青光眼的发病机制和诊治提供新的思路。

关键词： MicroRNA-155 原发性开角型青光眼小梁网细胞外基质