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patterning the neuronal cells via inkjet printing of self-assembled peptides on silk scaffolds

Progress in Natural Science:Materials International 2020年第5期30卷 686-696页

作者： Weizhen Sun Yi Zhang David A.Gregory Ana Jimenez-Franco Mhd Anas Tomeh Songwei Lv Jiqian Wang John W.Haycock Jian R.Lu Xiubo Zhao Department of Chemical and Biological Engineering University of Sheffield Department of Materials Science & Engineering University of Sheffield School of Pharmacy Changzhou University Centre for Bioengineering and Biotechnology China University of Petroleum East China School of Physics and Astronomy University of Manchester

The patterning of neuronal cells and guiding neurite growth are important for neuron tissue engineering and cell-based biosensors. In this paper, inkjet printing has been employed to pattern self-assembled I3QGK peptide nanofibers on silk substrates for guiding the growth of neuron-like PC12 cells. Atomic force microscopy(AFM)confirmed the dynamic self-assembly of I3QGK into nanofiber structures. The printed self-assembled peptide strongly adheres to regenerated silk fibroin(RSF) substrates through charge-charge interactions. It was observed that in the absence of I3QGK, PC12 cells exhibited poor attachment to RSF films, while for RSF surfaces coated or printed with peptide nanofibers, cellular attachment was significantly improved in terms of both cell density and morphology. AFM results revealed that peptide nanofibers can promote the generation of axons and terminal buttons of PC12 cells, indicating that I3QGK nanofibers not only promote cellular attachment but also facilitate differentiation into neuronal phenotypes. Inkjet printing allows complex patterning of peptide nanofibers onto RSF substrates, which enabled us to engineer cell alignment and provide an opportunity to direct axonal development in vitro. The live/dead assay showed that printed I3QGK patterns exhibit no cytotoxicity to PC12 cells demonstrating potential for future nerve tissue engineering applications.

关键词： Neurobiology micro-patterning Inkjet printing Self-assembling peptides PC12 cells

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Laser micro-Printing of Dye-Molecules on Polymeric Surfaces

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Open Journal of Applied Sciences 2016年第7期6卷 416-421页

作者： Wesley Sims Aschalew Kassu Carlton Farley Anup Sharma Department of Physics Chemistry and Mathematics Alabama A&M University Normal AL USA Department of Mechanical Engineering Civil Engineering and Construction Management Alabama A&M University Normal AL USA

We describe a technique for micro-patterning and immobilization of dyes on polymer substrates using a low-power visible laser for dye-excitation. Deposits from an aqueous medium containing the dye can be attached at any desired spot on the substrate simply by exposing the area to laser light. The area of the laser beam can control the spot-size of immobilized dye, in the range of 10 - 100 microns. The immobilization technique is characterized by micro-printing numerals, alphabets and patterns on polybutadiene substrates with Rhodamine (Rh6G) dye. Adsorption of laser-excited dye molecules within the polymer appears to be the mechanism for laser-printing technique.

关键词： Laser micro-Printing Laser Immobilization of Molecules micro-patterning Reticle

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