Patterning the neuronal cells via inkjet printing of self-assembled peptides on silk scaffolds
Patterning the neuronal cells via inkjet printing of self-assembled peptides on silk scaffolds作者机构：Department of Chemical and Biological Engineering University of Sheffield Department of Materials Science & Engineering University of Sheffield School of Pharmacy Changzhou University Centre for Bioengineering and Biotechnology China University of Petroleum East China School of Physics and Astronomy University of Manchester
出 版 物：《Progress in Natural Science:Materials International》 (自然科学进展·国际材料(英文))
年 卷 期：2020年第30卷第5期
页 面：686-696页
核心收录：
学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0836[工学-生物工程]
基 金：the EPSRC (EP/N007174/1 and EP/N023579/1) Royal Society (RG160662 and IE150457) Jiangsu specially-appointed professor program for support
主 题：Neurobiology Micro-patterning Inkjet printing Self-assembling peptides PC12 cells
摘 要：The patterning of neuronal cells and guiding neurite growth are important for neuron tissue engineering and cell-based biosensors. In this paper, inkjet printing has been employed to pattern self-assembled I3QGK peptide nanofibers on silk substrates for guiding the growth of neuron-like PC12 cells. Atomic force microscopy(AFM)confirmed the dynamic self-assembly of I3QGK into nanofiber structures. The printed self-assembled peptide strongly adheres to regenerated silk fibroin(RSF) substrates through charge-charge interactions. It was observed that in the absence of I3QGK, PC12 cells exhibited poor attachment to RSF films, while for RSF surfaces coated or printed with peptide nanofibers, cellular attachment was significantly improved in terms of both cell density and morphology. AFM results revealed that peptide nanofibers can promote the generation of axons and terminal buttons of PC12 cells, indicating that I3QGK nanofibers not only promote cellular attachment but also facilitate differentiation into neuronal phenotypes. Inkjet printing allows complex patterning of peptide nanofibers onto RSF substrates, which enabled us to engineer cell alignment and provide an opportunity to direct axonal development in vitro. The live/dead assay showed that printed I3QGK patterns exhibit no cytotoxicity to PC12 cells demonstrating potential for future nerve tissue engineering applications.
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