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Different Doses of the Enhanced UV-B Radiation Effects on Wheat Somatic Cell Division

CellBio 2015年第2期4卷 30-36页

作者： Feifeng Liu Huize Chen Rong Han College of Life Science Shanxi Normal University Linfen China Higher Education Key Laboratory of Plant Molecular and Environmental Stress Response (Shanxi Normal University) in Shanxi Province Linfen China

Being sessile, plants are continuously exposed to DNA-damaging agents presenting in the environment such as ultraviolet (UV). Sunlight acts as an energy source for photosynthetic plants;hence, avoidance of UV radiations (namely, UV-A, 315 - 400 nm;UV-B, 280 - 315 nm;and UV-C, 1 group: 4.05 kJ•m-2•d-1, B2 group: 10.08 kJ•m-2•d-1, B3 group: 7.05 kJ•m-2•d-1, B4 group: 23.02 kJ•m-2•d-1) treatment wheat, then, explored on the growth of wheat root and wheat root tip cell of chromosome aberration effect. In wheat, root-tip cells were observed with confocal laser scanning microscopy (CLSM), the results showed that low doses of B1 group (4.05 kJ•m-2•d-1) promoted the growth of wheat root and cell mitosis frequency. But high dose of B2 group (10.08 kJ•m-2•d-1), B3 group (17.05 kJ•m-2•d-1), B4 group (23.02 kJ•m-2•d-1) inhibited the growth of wheat root tip, and made crooked growth of wheat root, and inhibited the wheat root tip cell mitotic frequency and processed that induce root tip cells of wheat produce all kinds of aberration of chromosome in the interphase containing “multiple nucleoli nuclei”, “incomplete nuclei”, “long round nuclei”, “bean sprouts nucleus”. In mitosis M period contains “dissociative chromosome”, “chromosome bridge”, “adhesion chromosome”, “multi-bundle divide”, “nuclear anomalies”. After, high doses of enhanced UV-B radiation treatment, most of the cell cycle anomaly concentrated in mitosis interphase. In mitosis M period, with UV-B radiation dose enhanced chromosome aberration rate was on the rise and the aberration types also increasing.

关键词： Wheat Enhanced UV-B Radiation Chromosome Aberration Confocal Laser Scanning microscopy (CLSM)

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西南地区当归属植物叶表皮微形态研究

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四川大学学报（自然科学版） 2009年第6期46卷 1798-1804页

作者：冯图刘爽何兴金四川大学生命科学学院成都610065 毕节学院环境与生命科学系毕节551700

利用光学显微镜和扫描电镜对中国西南地区12种当归属植物的叶表皮微形态(如气孔器类型、表皮细胞与气孔形状、气孔纹饰及细胞角质膜特征等)进行了观察,测量并统计气孔长度和气孔指数.研究结果表明当归属植物叶表皮微形态特征在种间存在... 详细信息

利用光学显微镜和扫描电镜对中国西南地区12种当归属植物的叶表皮微形态(如气孔器类型、表皮细胞与气孔形状、气孔纹饰及细胞角质膜特征等)进行了观察,测量并统计气孔长度和气孔指数.研究结果表明当归属植物叶表皮微形态特征在种间存在较大的差异,但表皮细胞形状、气孔分布情况、气孔类型和细胞角质膜特征等是本属植物较为稳定的演化特征,可以作为物种分类与鉴别的依据.最后根据叶表皮微形态特征列出了12种当归属植物的分种检索表.

关键词：中国西南地区当归属植物叶表皮微形态形态研究 Southwest China characters species of 气孔指数 scanning electron microscopy 微形态特征表皮细胞 light microscopy 膜特征 significance 光学显微镜分种检索表演化特征扫描电镜气孔长度

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Effect of Different Calcination Process and Gd<sub>2</sub>O<sub>3</sub>as Impurities on the Different Phases of Bi-Based Superconductor

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World Journal of Condensed Matter Physics 2012年第3期2卷 148-152页

作者： Ahmad Amirabadizadeh Sara Memarzadeh Naser Tajabor Hadi Arabi Department of Physics Faculty of Science Fer-dowsi University of Mashhad Mashhad Iran Department of Physics Faculty of Science University of Birjand Birjand Iran

In this research two samples of a nominal composition Bi1.6Pb0.4Sr2Ca2Cu3Ox were prepared by the solid state reaction method, using two different routes. Codes A and B are used to refer to the two samples. In preparing sample A, the standard method for calcination was used, while for sample B, the SrCO3 and CaCO3 were calcinated at 1100℃ for 3 h. Calcination was done separately on SrCO3 and CaCO3 in order to eliminate CO2. Then after mixing and grinding, the powder mixtures of Bi2O3, CuO, PbO, SrO and CaO were calcinated at 840℃ for 70 h. Also, the samples (samples C) of nominal compositions Bi1.6Pb0.4Sr2Ca2Cu3Ox+ % x Gd2O3 (% x = 0, 3, 5, 7 and 9) have been synthesized by this method. The XRD and SEM results show that addition of Gd2O3 helps to increase the amount of Bi-2212 phase, while alternative calcination process improves the formation of the Bi-2223 phase. In addition, the AC susceptibility measurements confirm the XRD and SEM results.

关键词： Bi-Pb-Sr-Ca-Cu-O System X-Ray Diffraction Scanning Electron microscopy AC Susceptibility

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AFM Analysis on Polymer Optical Micro-Resonators: Investigation on Quality Factor Origin

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Optics and Photonics Journal 2013年第4期3卷 291-295页

作者： David Pluchon Nolwenn Huby Véronique Vié Pascal Panizza Bruno Bêche 1Institute of Physics of Rennes UMR CNRS 6251 University of Rennes 1 Campus Beaulieu Rennes France 2Institut Universitaire de France-IUF Paris France Institute of Physics of Rennes UMR CNRS 6251 University of Rennes 1Campus Beaulieu Rennes France

This paper deals with the surface analysis of spherical polymeric optical micro-resonators in order to correlate surface defects with optical characteristics. Atomic force microscopy was used on structures to determine surface quality, which is the main origin of optical scattering losses. Surface morphologies were numerically treated to enable a relevant investigation on surface parameters such as root mean square (RMS) roughness (30.1 +/- 3.0 nm) or correlation length (few microns) necessary to express optical quality factors. A statistical analysis was conducted for calibration of these parameters as a function of cavities’ diameter. Results are in perfect agreement with spectral analyses performed in parallel on others structures. This comparison highlights the main role of scattering losses on quality factor origin.

关键词： Optical Micro-Resonators Quality Factor Origin Atomic Force microscopy Surface Analysis Scattering Loss

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Microscopic Identification of Zhuang Medicine Laportea bulbifera(Sieb. et Zucc.) Wedd.

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Agricultural Biotechnology 2019年第2期8卷 191-192,195页

作者： Haicheng WEN Deqiang DOU Songji WEI Caiyue LIN Xuesong WANG Zhonghua DAI Wei WEI Guangxi University of Chinese Medicine College of Pharmacy Liaoning University of Traditional Chinese Medicine

[Objectives] This study was conducted to investigate the microscopic identification characteristics of Laportea bulbifera(Sieb. et Zucc.) Wedd. [Methods] The characteristics of L. bulbifera leaf, stem and powder were identified by microscopy. [Results] The main microscopic identification characteristics of the tissue structures of L. bulbifera leaf and stem at different positions and L. bulbifera powder were determined. [Conclusions] The microscopic identification results are characteristic, and could serve as the identification basis of L. bulbifera. These results could provide references for the quality control of L. bulbifera.

关键词： Zhuang medicine Laportea bulbifera (Sieb.et Zucc.) Wedd. microscopy Identification

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Deformation Nano-Mechanisms Occurring on the Interface of Dissimilar Materials Joined in Solid Phase by Means of High Temperature Rolling

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Open Journal of Metal 2014年第4期4卷 107-111页

作者： Boris V. Borts Nikolay I. Danilenko Aleksander A. Parkhomenko Viktor I. Tkachenko Irina M. Korotkova Igor O. Vorobyov Aleksander T. Lopata National Science Center Kharkov Institute of Physics & Technology The National Academy of Sciences of Ukraine (NASU) Kharkov Ukraine Frantsevich Institute for Problems of Materials Science The National Academy of Sciences of Ukraine (NASU) Kyiv Ukraine

Nano-mechanisms of crystal lattice deformation on the interface of dissimilar materials (Cu-Nb), joined by vacuum rolling in solid phase under high temperature (950℃) were studied by means of high resolution electron microscopy. Input of carriers of rotation modes—nano-dipoles of partial disclinations and nano-twist disclinations in the form of double spirals in deformation mechanisms were analyzed. The role of these mechanisms in decrease of shear stability of the interface areas and in production of high-quality rolled metal is discussed.

关键词： Vacuum Hot Rolling Solid Phase Interface Cupper-Niobium microscopy Disclinations

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Ultrastructural observation of tannic acid-captured exocytotic secretory granules in the anterior pituitary gland cells of the rat

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Journal of Medical Colleges of PLA(China) 1996年第3期11卷 217-219页

作者：林英华刘莹莹张旭鞠躬

Tannic acid has been employed to capture the exocytotic secretory granules by binding amino and carboxyl groups of secreted proteins and mordanting them into eletrondense material, and making them easily observed and precisely quantified by electron microscopy. The present study, using tannic acid-captured exocytotic method,aims to investigate ultrastructurally the secretion of the anterior pituitary gland cells of the rat. The exocytosis is frequently identified on lactotrophs and corticotrophs, but rarely somatotrophs,gonadotrophs and thyrotrophs. 12 lactotrophs and 12 corticotrophs of the anterior pituitary are randomly selected from each sample and the exocytotic secretory granules (ESG) are counted. The ESG(x) is 1. 1 6?. 1 6 in lactotrophs and 1. 21?0.24 in corticotrophs (P>0. 05). The results morphologically suggest that the lactotrophs,as well as corticotrophs strongly respond to the stress.

关键词： tannic acid exocytosis electron microscopy lactotroph corticotroph

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Joint Operation of Atomic Force Microscope and Advanced Laser Confocal Microscope for Observing Surface Processes in a Protein Crystal

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Journal of Surface Engineered Materials and Advanced Technology 2012年第3期2卷 210-214页

作者： Shin-ichiro Yanagiya Nobuo Goto Institute of Technology and Science University of Tokushima Tokushima Japan.

We demonstrated the insitu observation of a moving atomic force microscope (AFM) cantilever using a laser confocal microscope combined with a differential interference microscope (LCM-DIM). The AFM cantilever scanned or indented the {110} surface of a hen egg-white lysozyme crystal in a supersaturated solution. Using a soft cantilever, we could observe the step growth with high time resolution by LCM-DIM and perform quantitative measurements of the step height by AFM simultaneously. In addition, a hard cantilever was used with LCM-DIM to observe the dynamics of crystal surface scratching and indentation. In the supersaturated solution, the small steps generated from the scratched line aggregated to macro steps, and subsequently flattened the surface.

关键词： Laser Confocal microscopy Atomic Force microscopy Surface Process Protein Crystal

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Effects of procainamide on 0morphological parameter of dense granule and a granule in blood platelet

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Journal of Medical Colleges of PLA(China) 1999年第2期14卷 144-148页

作者：林继红单春文杨素勤刘连璞顾为望

Objective: To study the protective effect of procainamide (PA) on the ultrastructure of blood ***:The stereology measurement was used to study the effects of PA on morphological parameters of dense granule and a granule. Results: 8. 5, 34. 0 and 136. 0 μmol· L-1 PA ascended morphological parameter values of dense granule and a granule significantly. The increasing percentages were 153. 6% ~ 256. 2% (SS ), 81. 6% ~188. 9% (Sv) and 96. 3% ~145. 4 % (St ) for surface parameter values; 144. 0% ~499. 7% (Vv) and 221. 3% ~1593. 4 % (Vt ) for volume parameter values in dense granule. In a granule, the increasing percentages were 68. 2 %~335. 9% (Nv) and 14. 8%~ 696. 0% (N) for number parameter values; 45. 4%~ 87. 5% (S), -(50.7% 57. 1 % ) (SS), 160. 3% -558. 0% (Sv ) and 181. 5%~395. 7% (St ) for surface parameter values ; 87. 8%~127. 7% (D). 81. 4 %~ 202. 3% (V), 410. 5% ~773. 6% (Vv) and 195. 8% ~297. 8% (Vt) for volume parameter values. Conclusion: PA protects dense granule and a granule from AA-stimulating changes of morphology.

关键词： procainamide arachidonic acid blood platelet electron microscopy stereology measllrement

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Numerical Studies of Divided Aperture Techniques in Confocal System

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Journal of Power and Energy Engineering 2019年第11期7卷 42-46页

作者： Haonan Lai Guangzhou China

This paper performs a numerical study of the imaging process in confocal microscopy. We simulate the imaging result using Fresnel diffraction formula. We also consider the divided aperture technique in confocal system. Different aperture shapes were varied to achieve best performance in terms of resolution and integrated intensity. Here, the D-shaped achieves the best performance in x-axis resolution, while the annular aperture shape is the worst for integrated intensity. We propose using different aperture shapes for various biomedical applications.

关键词： Confocal microscopy Aperture Resolution Integrated Intensity

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