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Gene expression and metabolite profiles of cotton fiber during cell elongation and secondary cell wall synthesis

Cell Research 2007年第5期17卷 422-434页

作者： Jin-Ying Gou1,2,3 Ling-Jian Wang1,2 Shuang-Ping Chen4 Wen-Li Hu1,2 Xiao-Ya Chen1,2 1 National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China 2 CAS Center of Shanghai Chenshan Botanical Garden, 300 Fenglin Road, Shanghai 200032, China 3 Graduate School of Chinese Academy of Sciences, Shanghai 200032, China 4 Department of Computer Science and Technology, University of Science and Technology of China, Hefei 230026, China NationalKeyLaboratoryofPlantMolecularGenetics InstituteofPlantPhysiologyandEcologyShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciences300FenglinRoadShanghai200032China

Cotton fibers elongate rapidly after initiation of elongation, eventually leading to the deposit of a large amount ofcellulose. To reveal features of cotton fiber cells at the fast elongation and the secondary cell wall synthesis stages, wecompared the respective transcriptomes and metabolite profiles. Comparative analysis of transcriptomes by cDNA arrayidentified 633 genes that were differentially regulated during fiber development. Principal component analysis (PCA)using expressed genes as variables divided fiber samples into four groups, which are diagnostic of developmental *** grouping results are also found if we use non-polar or polar metabolites as variables for PCA of developingfibers. Auxin signaling, wall-loosening and lipid metabolism are highly active during fiber elongation, whereas cellulosebiosynthesis is predominant and many other metabolic pathways are downregulated at the secondary cell wall synthesisstage. Transcript and metabolite profiles and enzyme activities are consistent in demonstrating a specialization process ofcotton fiber development toward cellulose synthesis. These data demonstrate that cotton fiber cell at a certain stage has itsown unique feature, and developmental stages of cotton fiber cells can be distinguished by their transcript and metaboliteprofiles. During the secondary cell wall synthesis stage, metabolic pathways are streamed into cellulose synthesis.

关键词： cotton fiber transcriptome metabolite profile auxin cell elongation cellulose synthesis

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Salt stress-induced changes in soil metabolites promote cadmium transport into wheat tissues

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Journal of Environmental Sciences 2023年第5期127卷 577-588页

作者： Lifu Wang Luyao Qin Xiaoyi Sun Shuwen Zhao Lei Yu Shibao Chen Meng Wang Key Laboratory of Plant Nutrition and Fertilizer Ministry of Agriculture and Rural Affairs/Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081China

Soil salinity is known to improve cadmium(Cd)mobility,especially in arid ***,the mechanisms involved in how salt stress-associated metabolic profiles participate in mediating Cd transport in the soil-plant system remain poorly *** study was designed to investigate the effects of salinity-induced changes in soil metabolites on Cd *** salts in different combinations according to molar ratio(NaCl:Na_(2)SO_(4)=1:1;NaCl:Na_(2)SO_(4):NaHCO_(3)=1:2:1;NaCl:Na_(2)SO_(4):NaHCO_(3):Na_(2)CO_(3)=1:9:9:1;NaCl:Na_(2)SO_(4):NaHCO_(3):Na_(2)CO_(3)=1:1:1:1)were applied to the Cd-contaminated soils,which increased soil Cd availability by 22.36%and the Cd content in wheat grains by 36.61%,compared to the *** stress resulted in soil metabolic reprogramming,which might explain the decreased growth of wheat plants and increased Cd transport from the soil into wheat *** example,down-regulation of starch and sucrose metabolism reduced the production of sugars,which adversely affected growth;up-regulation of fatty acid metabolism allowed wheat plants to maintain a normal intracellular environment under saline conditions;up-regulation of the tricarboxylic acid(TCA)cycle was triggered,causing an increase in organic acid synthesis and the accumulation of organic acids,which facilitated the migration of soil Cd into wheat *** summary,salt stress can facilitate Cd transport into wheat tissues by the direct effect of salt-based ions and the combined effect of altered soil physicochemical properties and soil metabolic profiles in Cd-contaminated soils.

关键词： Saline stress Cadmium availability Wheat growth metabolite profile Rhizosphere

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Metabolic Response of Pakchoi Leaves to Amino Acid Nitrogen

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Journal of Integrative Agriculture 2014年第4期13卷 778-788页

作者： WANG Xiao-li YU Wen-juan ZHOU Qian HAN Rui-feng HUANG Dan-feng Department of Horticulture School of Agriculture and BiologyShanghai Jiao Tong University Instrumental Analysis Center Shanghai Jiao Tong University

Different nitrogen （N） forms may cause changes in the metabolic profiles of plants. However, few studies have been conducted on the effects of amino acid-N on plant metabolic profiles. The main objective of this study was to identify primary metabolites associated with amino acid-N （Gly, Gln and Ala） through metabolic profile analysis using gas chromatography- mass spectrometry （GC-MS）. Plants of pakchoi （Brassica campestris L. ssp. chinensis L.）, Huawang and Wuyueman cultivars, were grown with different nitrogen forms （i.e., Gly, Gln, Ala, NO3-N, and N starvation） applied under sterile hydroponic conditions. The fresh weight and plant N accumulation of Huawang were greater than those of Wuyueman, which indicates that the former exhibited better N-use efficiency than the latter. The physiological performances of the applied N forms were generally in the order of NO3-N〉Gln〉Gly〉Ala. The metabolic analysis of leaf polar extracts revealed 30 amino acid N-responsive metabolites in the two pakchoi cultivars, mainly consisting of sugars, amino acids, and organic acids. Changes in the carbon metabolism of pakchoi leaves under amino acid treatments occurred via the accumulation of fructose, glucose, xylose, and arabinose. Disruption of amino acid metabolism resulted in accumulation of endogenous Gly in Gly treatment, Pro in Ala treatment, and Asn in three amino acid （Gly, Gln and Ala） treatments. By contrast, the levels of endogenous Gln and Leu decreased. However, this reduction varied among cultivars and amino acid types. Amino acid-N supply also affected the citric acid cycle, namely, the second stage of respiration, where leaves in Gly, Gln and Ala treatments contained low levels of malic, citric and succinic acids compared with leaves in NO3-N treatments. No significant difference in the metabolic responses was observed between the two cultivars which differed in their capability to use N. The response of primary metabolites in pakchoi leaves to amino acid-N supply may

关键词： amino acid metabolite profile pakchoi

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