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Journal of Environmental Sciences 2018年第3期30卷 356-366页

作者： Limei Xu Chongmiao Zhang Pengcheng Xu Xiaochang C.Wang International Science and Technology Cooperation Center for Urban Alternative Water Resources Development China Key Laboratory of Northwest Water Resource Environment and Ecology MOE China Engineering Technology Research Center for Wastewater Treatment and Reuse Shaanxi Province China School of Environmental and Municipal Engineering Xi'an University of Architecture and Technology Xi'an 710055 China

Traditional culture methods may underestimate the tolerance of microorganisms to disinfectants because of the existence of viable but nonculturable or sublethally injured cells after *** selection of a strict method is crucial for the evaluation of disinfection *** actions of 2 typical disinfectants–ultraviolet（UV）and chlorine–on the fecal indicator Escherichia coli were investigated by the detection of culturability,membrane permeability,metabolic activity,deoxyribonucleic acid（DNA）,and messenger ribonucleic acid（m RNA）.During UV disinfection,the irreversible damages in the cell membrane and cellular adenosine triphosphate（ATP）were negligible at low UV doses（80 m J/cm^2）.However,membrane permeability was damaged at low doses of chlorine（5 mg/L）,leading to leakage of cellular *** study showed that a slight lesion in DNA was detected even at high doses of UV（400 m J/cm^2）and chlorine（5 mg/L）*** decay of m RNA was more rapid than that of *** degradation level of m RNA depended on the choice of target *** exposure to 50 m J/cm^2UV dose or 5 mg/L chlorine for30 min,the DNA damage repair function（Rec A m RNA）was *** m RNA involved in the DNA damage repair function can be a potential indicator of bacterial viability.

关键词： UV Chlorination membrane damage Adenosine triphosphate DNA mRNA

Antimicrobial activity and mode of action of terpene linalyl anthranilate against carbapenemase-producing Klebsiella pneumoniae

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Journal of Pharmaceutical Analysis 2021年第2期11卷 210-219页

作者： Shun-Kai Yang Khatijah Yusoff Mokrish Ajat Wai-Sum Yap Swee-Hua Erin Lim Kok-Song Lai Department of Cell and Molecular Biology Faculty of Biotechnology and Biomolecular SciencesUniversiti Putra Malaysia43400SerdangSelangorMalaysia Department of Microbiology Faculty of Biotechnology and Biomolecular SciencesUniversiti Putra Malaysia43400SerdangSelangorMalaysia Department of Veterinary Preclinical Sciences Faculty of Veterinary MedicineUniversiti Putra Malaysia43400SerdangSelangorMalaysia Department of Biotechnology Faculty of Applied SciencesUCSI University56000CherasKuala LumpurMalaysia Health Sciences Division Abu Dhabi Women’s CollegeHigher Colleges of Technology41012Abu DhabiUnited Arab Emirates

Mining of plant-derived antimicrobials is the major focus at current to counter antibiotic resistance. This study was conducted to characterize the antimicrobial activity and mode of action of linalyl anthranilate(LNA) against carbapenemase-producing Klebsiella pneumoniae(KPC-KP). LNA alone exhibited bactericidal activity at 2.5%(V/V), and in combination with meropenem(MPM) at 1.25%(V/V). Comparative proteomic analysis showed a significant reduction in the number of cytoplasmic and membrane proteins,indicating membrane damage in LNA-treated KPC-KP cells. Up-regulation of oxidative stress regulator proteins and down-regulation of oxidative stress-sensitive proteins indicated oxidative stress. Zeta potential measurement and outer membrane permeability assay revealed that LNA increases both bacterial surface charge and membrane permeability. Ethidium bromide influx/efflux assay showed increased uptake of ethidium bromide in LNA-treated cells, inferring membrane damage. Furthermore, intracellular leakage of nucleic acid and proteins was detected upon LNA treatment. Scanning and transmission electron microscopies again revealed the breakage of bacterial membrane and loss of intracellular materials. LNA was found to induce oxidative stress by generating reactive oxygen species(ROS) that initiate lipid peroxidation and damage the bacterial membrane. In conclusion, LNA generates ROS, initiates lipid peroxidation, and damages the bacterial membrane, resulting in intracellular leakage and eventually killing the KPC-KP cells.

关键词： Comparative proteomic KPC-KP Linalyl anthranilate membrane damage ROS

Sensitivity of Different Cytotoxic Responses of Vero Cells Exposed to Organic Chemical Pollutants and their Reliability in the Bio-toxicity Test of Trace Chemical Pollutants

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Biomedical and Environmental Sciences 2010年第3期23卷 219-229页

作者： TING-TING LIAO YAN-LING SHI JIAN-WEI JIA AND LEI WANG College of Environmental Science and Engineering State Key Laboratory of Pollution Control and Resource Reuse Tongji University Shanghai 200092 China

Objective To find a sensitive cytotoxic response to reflect the bio-toxicity of trace organic pollutants, the sensitivity and reliability of morphological change and proliferation inhibition of Vero cells exposed to 2, 4, 6-trichlorophenol （TCP） and the leachate from products related to drinking water （PRDW） were compared, and the mechanism of the morphological change in Vero cells exposed to chemical pollutants was studied. Methods Vero cells were treated by different concentration of TCP and the leachate from PRDW. Methylthiazol-2-yl-2, 5-diphenyl tetrazolium bromide （MTT） assay was carried out for proliferation inhibition. Bioluminescence method was carried out as another method to test the toxicity of TCP. Flow Cytometry assay was used to test cell Apoptosis and damage of cell-membrane. Results 0.25 mg/L TCP had an effect on cell morphology, and the proportion of morphologically changed cells increased with increasing TCP concentration. At low TCP concentrations, inhibition of cell proliferation did not seem to correlate to TCP concentration, and was negative when TCP concentration was 〈1.0 mg/L. After exposure to leachate from PRDW extracted at different temperatures, the percentage of morphologically changed cells increased with extracting temperature, but the inhibition of cell proliferation failed to reflect the correlation between extracting temperature and proliferation inhibition of Vero cells. Although the Sensitivity of bioluminescence method seems to be similar to morphological change in Veto cells, the bacterial in this method is not homologous enough with human body cells to reflect the toxicity to human body. These imply cell morphological change is a more sensitive and reliable method to reflect bio-toxicity of organic pollutants than proliferation inhibition. Flow cytometry analysis and cell rejuvenation experiments indicated cell membrane damage, which results in cell morphological change, was an early and sensitive cytotoxic response comparing w

关键词： Vero cell Morphological change membrane damage Bio-toxicity test Drinking water

Unique Azolyl Acylhydrazonyl Hybridization of Aloe Emodins to Access Potential Antibacterial Agents

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Chinese Journal of Chemistry 2024年第15期42卷 1741-1758页

作者： Yi-Xin Wang Zhao Deng Aisha Bibi Bo Fang Cheng-He Zhou Institute of Bioorganic&Medicinal Chemistry Key Laboratory of Applied Chemistry of Chongqing MunicipalitySchool of Chemistry and Chemical EngineeringSouthwest UniversityChongqing 400715China College of Pharmacy National&Local Joint Engineering Research Center of Targeted and Innovative TherapeuticsChongqing Key Laboratory of Kinase Modulators as Innovative MedicineChongqing University of Arts and SciencesChongqing 402160China

A type of unique azole-hybridized acylhydrazonyl aloe emodins(AAEs)were developed as new antibacterial agents for combating bacterial *** target AAEs showed strong antibacterial activities,especially,tetrazolylthioether AAE 27a exhibited broad antibacterial spectrum with 16-256 folds and 8-64 folds more active antibacterial efficacy than the reference drugs aloe emodin and norfloxacin,*** AAE 27a also gave low hemolysis and cytotoxicity,as well as favorable *** mechanism explorations revealed that tetrazolylthioether AAE 27a could cause bacterial membrane depolarization and damage the cell membrane,resulting in nucleic acid ***,compound 27a could intercalate into DNA to impede its replication and form supramolecular 27a-DNA gyrase complex to disturb the function of DNA *** findings would provide valuable insights for the further exploration of azolyl acylhydrazonyl aloe emodins as new potential antibacterial candidates.

关键词： Aloe emodin Hydrazone Heterocycle Antibacterial membrane damage

Perturbation of clopyralid on bio-denitrification and nitrite accumulation:Long-term performance and biological mechanism

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Environmental Science and Ecotechnology 2022年第1期9卷 27-39页

作者： Suyun Sun Ya-Nan Hou Wei Wei Hafiz Muhammad Adeel Sharif Cong Huang Bing-Jie Ni Haibo Li Yuanyuan Song Caicai Lu Yi Han Jianbo Guo Tianjin Key Laboratory of Aquatic Science and Technology School of Environmental and Municipal EngineeringTianjin Chengjian UniversityTianjin300384China National Technology Innovation Center of Synthetic Biology Tianjin Institute of Industrial BiotechnologyChinese Academy of SciencesTianjin300308China Centre for Technology in Water and Wastewater School of Civil and Environmental EngineeringUniversity of Technology SydneySydneyNSW2007Australia College of Chemistry and Environmental Engineering Shenzhen UniversityShenzhen518060China

The contaminant of herbicide clopyralid(3,6-dichloro-2-pyridine-carboxylic acid,CLP)poses a potential threat to the ecological ***,there is a general lack of research devoted to the perturbation of CLP to the bio-denitrification process,and its biological response mechanism remains ***,long-term exposure to CLP was systematically investigated to explore its influences on denitrification performance and dynamic microbial *** showed that low-concentration of CLP(membrane damage,resulting in nitrite ***,after more than 80 days of acclimation,almost no nitrite accumulation was found at 60 mg/L *** was proposed that the secretion of extracellular polymeric substances(EPS)increased from 75.03 mg/g VSS at 15 mg/L CLP to 109.97 mg/g VSS at 60 mg/L CLP,which strengthened the protection of microbial cells and improved NIR activity and metabolic ***,the biodiversity and richness of the microbial community experienced a U-shaped *** relative abundance of denitrification-and carbon metabolism-associated microorganisms decreased initially and then recovered with the enrichment of microorganisms related to the secretion of EPS and N-acyl-homoserine lactones(AHLs).These microorganisms protected microbe from toxic substances and regulated their interactions among interand *** study revealed the biological response mechanism of denitrification after successive exposure to CLP and provided proper guidance for analyzing and treating herbicide-containing wastewater.

关键词： Denitrification Nitrite accumulation Clopyralid membrane damage Metabolism activity Microbial community

Effects of High-Temperature Stress on Photosynthetic Characteristics and Antioxidant Enzyme System of Paeonia ostii

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Phyton-International Journal of Experimental Botany 2022年第3期91卷 599-615页

作者： Xiaoxiao Wang Ziwen Fang Daqiu Zhao Jun Tao College of Horticulture and Plant Protection Yangzhou UniversityYangzhou225009China Joint International Research Laboratory of Agriculture and Agri-Product Safety The Ministry of Education of ChinaYangzhou UniversityYangzhou225009China

Paeonia ostii is an economically important oil crop,which has been widely cultivated in the middle and lower reaches of the Yangtze River in China in recent *** *** is highly adaptable to the environment,the prolonged high summer temperature in this region severely inhibits its growth,which adversely affects seed yield and *** this study,*** plants were subjected to 20℃/15℃(day/night)and 40℃/35℃(day/night)temperatures for 15 *** changes in physiological and biochemical indicators of *** under high-temperature stress were initially *** results showed that with the deepening of leaf etiolation,chlorophyll a and chlorophyll b concentration,carotenoid concentration,Soil Plant Analysis Development(SPAD)values and leaf relative water content decreased significantly,while both relative electrical conductivity(REC)and free proline concentration showed an upward ***,the continuous accumulation of reactive oxygen species(ROS)in *** plants,led to an increased activity of antioxidant enzymes including superoxide dismutase(SOD),peroxidase(POD),catalase(CAT)and ascorbate peroxidase(APX).Moreover,with the extension of the high-temperature treatment,the anatomical structures of *** were destroyed,resulting in a decreased photochemical efficiency of the photosystem II(PSII)reaction center and photosynthesis was *** together,these results provide reference values for understanding the physiological response of *** to hightemperature stress and establish a foundation for further research on the relevant underlying molecular mechanisms.

关键词： Antioxidant enzymes membrane damage photosynthesis reactive oxygen species

Postharvest Application of 1-Methylcyclopropene Maintains Quality and Extends the Shelf-Life of Zizania latifolia during Storage at 25˚C

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Agricultural Sciences 2023年第11期14卷 1501-1515页

作者： Xiuzi Tong Yanan Chen Nan Shi Mohamed Hawali Bata Gouda Xiaoxue Kong Kai Jiang Haibo Luo School of Food Science and Pharmaceutical Engineering Nanjing Normal University Nanjing China Faculty of Food Science Zhejiang Pharmaceutical University Ningbo China

The effects of 1-methylcyclopropene (1-MCP) on postharvest quality of Zizania latifolia during storage at 25˚C were investigated. The results pointed out that a postharvest application of 1-MCP maintained the good visual appearance of fresh Z. latifolia, inhibited browning, mildew and weight loss at the bottom of Z. latifolia, and there is no significant changes on L, a*, b* and ΔE during the whole storage period. In addition, 1-MCP treatment inhibited the respiratory intensity of Z. latifolia during the first three days of storage, but it was significantly higher than that of the control on the sixth day of storage. Compared with the control, 1-MCP treatment maintained relatively high SOD, CAT, APX activities and low PAL, POD, PLD, lipase and LOX activities, delayed the decline of AsA content, reduced the accumulation of O2-, H2O2 and MDA, and ultimately maintained the integrity of cell structure and delayed the senescence of Z. latifolia. In addition, positive effects of 1-MCP on maintaining the cell structure integrity were observed in this investigation throughout the storage period at 25˚C.

关键词： Zizania latifolia 1-Methylcyclopropene Browning Enzyme membrane damage

Physiological Changes in Leaves of Medicago falcata Under Low-Temperature Stress

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Journal of Northeast Agricultural University(English Edition) 2023年第2期30卷 78-88页

作者： Yang Mei Li Han Zhang Yu Wang Zhao-yu Chai Lu Ding De-shun Zhang Pan Cui Guo-wen College of Animal Sciences and Technology Northeast Agricultural UniversityHarbin 150030China

Low temperature significantly restricts crop yield and *** falcata(***)is a typical legume species that exhibits great capacity of tolerance to low *** understand the low-temperature responses in ***,the electrolyte leakage and lipid peroxidation level,and activities of superoxide dismutase(SOD),catalase(CAT)and peroxidase(POD),and contents of reduced glutathione(GSH),soluble protein,soluble sugar and proline were investigated in low-temperature-stressed *** *** electrolyte leakage and malondialdehyde(MDA)content increased,and could be used to quantify low-temperature damage at cellular *** then,the significant change of SOD,POD and CAT activities,and GSH content reflected the higher reactive oxygen species(ROS)scavenging capacity in *** addition,the significant change of soluble protein,soluble sugar and proline contents helped to maintain osmotic equilibrium,energy supply and protein *** nine physiological traits were analyzed by gray relational grade analysis and ranked from the highest to the lowest as follows:electrolyte leakage,GSH,proline,soluble protein,MDA,soluble sugar,SOD,CAT and POD,and illustrated that the electrolyte leakage level,GSH and proline contents should be selected and measured priority in *** low-temperature tolerance to improve measurement efficiency.

关键词： antioxidant responses gray relational grade analysis cold response membrane damage osmotic adjustment