Perturbation of clopyralid on bio-denitrification and nitrite accumulation:Long-term performance and biological mechanism

作     者：Suyun Sun Ya-Nan Hou Wei Wei Hafiz Muhammad Adeel Sharif Cong Huang Bing-Jie Ni Haibo Li Yuanyuan Song Caicai Lu Yi Han Jianbo Guo 

作者机构：Tianjin Key Laboratory of Aquatic Science and TechnologySchool of Environmental and Municipal EngineeringTianjin Chengjian UniversityTianjin300384China National Technology Innovation Center of Synthetic BiologyTianjin Institute of Industrial BiotechnologyChinese Academy of SciencesTianjin300308China Centre for Technology in Water and WastewaterSchool of Civil and Environmental EngineeringUniversity of Technology SydneySydneyNSW2007Australia College of Chemistry and Environmental EngineeringShenzhen UniversityShenzhen518060China 

出 版 物：《Environmental Science and Ecotechnology》 (环境科学与生态技术（英文）)

年 卷 期：2022年第9卷第1期

页      面：27-39页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：the National Natural Science Foundation of China(Grant No.52000134) China Postdoctoral Science Foundation(No.2020M680894) Tianjin Science and Technology Program(No.21YDTPJC00700) Natural Science Foundation of Tianjin Science and Technology Correspondent Project(No.19JCQNJC07800) Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project(No.TSBICIP-CXRC-007) 

主　　题：Denitrification Nitrite accumulation Clopyralid Membrane damage Metabolism activity Microbial community 

摘      要：The contaminant of herbicide clopyralid(3,6-dichloro-2-pyridine-carboxylic acid,CLP)poses a potential threat to the ecological ***,there is a general lack of research devoted to the perturbation of CLP to the bio-denitrification process,and its biological response mechanism remains ***,long-term exposure to CLP was systematically investigated to explore its influences on denitrification performance and dynamic microbial *** showed that low-concentration of CLP(15 mg/L)caused severe nitrite accumulation initially,while higher concentrations(35e60 mg/L)of CLP had no further effect after long-term *** mechanistic study demonstrated that CLP reduced nitrite reductase(NIR)activity and inhibited metabolic activity(carbon metabolism and nitrogen metabolism)by causing oxidative stress and membrane damage,resulting in nitrite ***,after more than 80 days of acclimation,almost no nitrite accumulation was found at 60 mg/L *** was proposed that the secretion of extracellular polymeric substances(EPS)increased from 75.03 mg/g VSS at 15 mg/L CLP to 109.97 mg/g VSS at 60 mg/L CLP,which strengthened the protection of microbial cells and improved NIR activity and metabolic ***,the biodiversity and richness of the microbial community experienced a U-shaped *** relative abundance of denitrification-and carbon metabolism-associated microorganisms decreased initially and then recovered with the enrichment of microorganisms related to the secretion of EPS and N-acyl-homoserine lactones(AHLs).These microorganisms protected microbe from toxic substances and regulated their interactions among interand *** study revealed the biological response mechanism of denitrification after successive exposure to CLP and provided proper guidance for analyzing and treating herbicide-containing wastewater.