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Polymodal Functionality of C. elegans OLL Neurons in mechanosensation and Thermosensation

Neuroscience Bulletin 2021年第5期37卷 611-622页

作者： Yuedan Fan Wenjuan Zou Jia Liu Umar Al-Sheikh Hankui Cheng Duo Duan Du Chen Siyan Liu Luyi Chen Jilei Xu Firdosh Ruhomutally Lijun Kang Department of Neurobiology and Department of Neurosurgery of the First Affiliated Hospital Zhejiang University School of MedicineHangzhou 310053China NHC and CAMS Key Laboratory of Medical Neurobiology MOE Frontier Science Center for Brain Research and Brain-Machine IntegrationSchool of Brain Science and Brain MedicineZhejiang UniversityHangzhou 310053China Department of Gastroenterology Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016China Department of Human Sciences and Psychology University of South Africa(UNISA)Pretoria 0003South Africa

Sensory modalities are important for survival but the molecular mechanisms remain challenging due to the polymodal functionality of sensory neurons. Here, we report the C. elegans outer labial lateral(OLL) sensilla sensory neurons respond to touch and cold. mechanosensation of OLL neurons resulted in cell-autonomous mechanically-evoked Ca~(2+) transients and rapidly-adapting mechanoreceptor currents with a very short *** of OLL neurons might be carried by a novel Na~+ conductance channel, which is insensitive to amiloride. The bona fide mechano-gated Na~+ -selective degenerin/epithelial Na~+ channels, TRP-4, TMC, and Piezo proteins are not involved in this ***, OLL neurons also mediated cold but not warm responses in a cell-autonomous manner. We further showed that the cold response of OLL neurons is not mediated by the cold receptor TRPA-1 or the temperaturesensitive glutamate receptor GLR-3. Thus, we propose the polymodal functionality of OLL neurons in mechanosensation and cold sensation.

关键词： Polymodal sensory neuron mechanosensation Thermosensation Sodium channel Cold receptor OLL neurons

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Loss of Aβ-nerve endings associated with the Merkel cell-neurite complex in the lesional oral mucosa epithelium of lichen planus and hyperkeratosis

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International Journal of Oral Science 2016年第1期8卷 32-38页

作者： Daniela Calderon Carrion Yiiksel Korkmaz Britta Cho Marion Kopp Wilhelm Bloch Klaus Addicks Wilhelm Niedermeier Department of Prosthetic Dentistry Dental SchoolUniversity of CologneCologneGermany Center for Biochemistry Institute for Experimental Dental Research and Oral Musculoskeletal BiologyMedical FacultyUniversity of CologneCologneGermany Department of Anatomy University of CologneCologneGermany Department of Molecular and Cellular Sport Medicine German Sport UniversityCologneGermany

The Merkel cell-neurite complex initiates the perception of touch and mediates Ap slowly adapting type I responses. Lichen planus is a chronic inflammatory autoimmune disease with T-cell-mediated inflammation, whereas hyperkeratosis is characterized with or without epithelial dysplasia in the oral mucosa. To determine the effects of lichen planus and hyperkeratosis on the Merkel cell-neurite complex, healthy oral mucosal epithelium and lesional oral mucosal epithelium of lichen planus and hyperkeratosis patients were stained by immunohistochemistry （the avidin-biotin-peroxidase complex and double immunofluorescence methods）using pan cytokeratin, cytokeratin 20 （K20, a Merkel cell marker）, and neurofilament 200 （NF200, a myelinated Aβ- and Aδ-nerve fibre marker） antibodies. N F200-immunoreactive （ir） nerve fibres in healthy tissues and in the lesional oral mucosa epithelium of lichen planus and hyperkeratosis were counted and statistically analysed. In the healthy oral mucosa, K20-positive Merkel cells with and without close association to the intraepithelial NF200-ir nerve fibres were detected. In the lesional oral mucosa of lichen planus and hyperkeratosis patients, extremely rare NF200-ir nerve fibres were detected only in the lamina propria. Compared with healthy tissues, lichen planus and hyperkeratosis tissues had significantly decreased numbers of NF200-ir nerve fibres in the oral mucosal epithelium. Lichen planus and hyperkeratosis were associated with the absence of Aβ-nerve endings in the oral mucosal epithelium. Thus, we conclude that mechanosensation mediated by the Merkel cell-neurite complex in the oral mucosal epithelium is impaired in lichen planus and hvperkeratosis.

关键词： Aβ-nerve fibres hyperkeratosis lichen planus mechanosensation Merkel cell-neurite complex

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Demystifying Mechanosensitive Piezo Ion Channels

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Neuroscience Bulletin 2016年第3期32卷 307-309页

作者： X.Z.Shawn Xu Life Sciences Institute and Department of Molecular and Integrative Physiology University of Michigan

Mechanosensitive channels mediate touch,hearing,proprioception,and blood pressure *** proteins,including Piezo1 and Piezo2,represent a new class of mechanosensitive channels that have been reported to play key roles in most,if not all,of these *** structural architecture and molecular mechanisms by which Piezos act as mechanosensitive channels,however,remain *** new studies have now provided critical insights into the atomic structure and molecular basis of the ion permeation and mechano-gating properties of the Piezo1 channel.

关键词： mechanosensation Mechanosensory echanotransduction Mechanical Cryo-EM Piezo

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Analysis of Freely Swimming C. elegans Using Laser Diffraction

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Open Journal of Biophysics 2012年第3期2卷 101-107页

作者： Jenny Magnes Kathleen M. Raley-Susman Noureddine Melikechi Alicia Sampson Rebecca Eells Alexandra Bello Michael Lueckheide Department of Biology Vassar College Poughkeepsie USA Department of Physics and Pre-Engineering Delaware State University Dover USA Physics and Astronomy Department Vassar College Poughkeepsie USA

Soil and aquatic multicellular microorganisms play a critical role in the nutrient-cycling and organismal ecology of soil and aquatic ecosystems. These organisms live and behave in a complex three-dimensional environment. Most studies of microorganismal behavior, in contrast, have been conducted using microscope-based approaches, which limit the movement and behavior to a narrow, nearly two-dimensional focal field. We report on a novel analytical approach that provides real-time analysis of freely swimming C elegans without dependence on microscope-based equipment. This approach consists of tracking the temporal periodicity of diffraction patterns generated by directing laser light onto nematodes in a cuvette. We measured oscillation frequencies for freely swimming nematodes in cuvettes of different sizes to provide different physical constraints on their swimming. We compared these frequencies with those obtained for nematodes swimming within a small droplet of water on a microscope slide, a strategy used by microscope-based locomotion analysis systems. We collected data from diffraction patterns using two methods: video analysis and real time data acquisition using a fast photodiode. Swimming frequencies of nematodes in a droplet of ionic solution on a microscope slide was confirmed to be 2.00 Hz with a variance of 0.05 Hz for the video analysis method and 0.03 Hz for the real time data acquisition using a photodiode;this result agrees with previously published estimates using microscope-based analytical techniques. We find the swimming frequency of unconstrained worms within larger cuvettes to be 2.37 Hz with a variance of 0.02 Hz. As the cuvette size decreased, so did the oscillation frequency, indicating a change in locomotion when physical constraints are introduced.

关键词： Diffraction C. elegans Fourier Transform Fourier Analysis mechanosensation

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Drosophila Nomp C is a mechano-transduction channel subunit for gentle touch sensation

Drosophila Nomp C is a mechano-transduction channel subunit ...

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中国生理学会第24届全国会员代表大会暨生理学学术大会

作者： Yuh Nung Jan 闫致强 Wei Zhang 复旦大学生命科学学院

Touch sensation is essential for behaviors ranging from environmental exploration to social interaction,but the underlying mechanisms are largely *** Drosophila larvae,two types of sensory neurons,class ⅡI and class IV dendritic arborization（da）neurons,tile the body *** mechanotransduction channel Piezo in class IV da neurons is essential for sensing noxious mechanical stimulus but is not involved in gentle *** we report that class ⅡI da neurons are touch sensitive and contribute to gentle touch sensation,based on electrophysiological recording,calcium imaging and behavioral *** further identify Nomp C（no mechanoreceptor potential C）,a member of the transient receptor potential（TRP）family of ion channels,as a mechanotransduction channel for gentle *** C is highly expressed in class ⅡI da neurons and required for their ***,ectopic Nomp C expression confers touch sensitivity to the normally touch insensitive class IV da *** only is Nomp C critical for gentle touch to elicit behavioral responses,Nomp C expression in Drosophila S2cells gives rise to mechanosensitive channels whose ion selectivity can be altered by Nomp C mutation,indicating Nomp C is a pore-forming subunit of a mechanotransduction *** study establishes Nomp C as a bona fide mechanotransduction channel that satisfies all four criteria and mediates gentle touch sensation,and suggests that different mechanosensitive channels might be utilized to sense gentle touch versus noxious mechanical stimulus.

关键词： touch Drosophila ion channel mechanosensation

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