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Ultrasound-assisted isolation: A new method to isolate stromal vascular fraction

Chinese Journal of Plastic and Reconstructive Surgery 2024年第1期6卷 8-15页

作者： Yiming Gao Xiaojie Zhang Poh-Ching Tan Yun Xie Peiqi Zhang Tianyu Zhang Qingfeng Li Shuangbai Zhou 1.Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine 2.Department of Burns and Plastic Surgery, Shanghai Changzheng Hospital 3.College of Life Sciences, Shanghai Normal University Department of Plastic and Reconstructive Surgery Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Department of Burns and Plastic Surgery Shanghai Changzheng Hospital College of Life Sciences Shanghai Normal University

Background: The stromal vascular fraction(SVF), a cluster of stem and progenitor cells isolated from adipose tissue, holds significant promise for application in regenerative medicine. However, the existing methods for SVF isolation are time-consuming and expensive. Thus, in this study, we explored a new method of SVF extraction—ultrasound-assisted SVF isolation(USASI)—and compared the viability and characteristics of SVF isolated using different methods.Methods: SVF extraction methods using different combinations of ultrasound power, ultrasound time, collagenase dosage, and collagenase digestion time were compared with those of the control group(collagenase digestion method). The cell yield and vitality of the SVF were evaluated via cell counting and trypan blue staining. The cell components and immunophenotypes of freshly isolated SVF were analyzed using flow cytometry. The proliferative capacity and differentiation potential of the SVF were also identified.Results: Ultrasonication at 95 W-20 k Hz for 30 s followed by digestion with 0.15% collagenase for 30 min was identified as the most suitable parameter for the USASI method in isolating SVF, as recommended based on the evaluation of various tested conditions. The USASI method significantly reduced the collagenase dosage and shortened the digestion time. Compared to the collagenase digestion method, the USASI method had a higher cell yield and cell viability, with no adverse effects on cell components, proliferative capacity, or multipotential differentiation capacity.Conclusion: With reduced processing time, lower collagenase dosage, and increased cell yield without impairing the viability and characteristics of SVF, USASI holds the potential to emerge as a time-saving and cost-effective method for future clinical applications.

关键词： Stromal vascular fractions SVF isolation mechanical force Ultrasound

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Targeted mechanical forces enhance the effects of tumor immunotherapy by regulating immune cells in the tumor microenvironment

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Cancer Biology & Medicine 2023年第1期20卷 44-55页

作者： Pengfei Zhu Hongrui Lu Mingxing Wang Ke Chen Zheling Chen Liu Yang Cancer Center Department of Medical OncologyZhejiang Provincial People's Hospital(Affiliated People's HospitalHangzhoul Medical College)Hangzhou 310014China Graduate School of Clinical Medicine Bengbu Medical CollegeBengbu 233000China Department of Gastroenterology&Pancreatic Surgery Zhejiang Provincial People's HospitalHangzhou 310014China

mechanical forces in the tumor microenvironment(TME)are associated with tumor growth,proliferation,and drug resistance.Strong mechanical forces in tumors alter the metabolism and behavior of cancer cells,thus promoting tumor progression and metastasis.mechanical signals are transformed into biochemical signals,which activate tumorigenic signaling pathways through mechanical transduction.Cancer immunotherapy has recently made exciting progress,ushering in a new era of“chemo-free”treatments.However,immunotherapy has not achieved satisfactory results in a variety of tumors,because of the complex tumor microenvironment.Herein,we discuss the effects of mechanical forces on the tumor immune microenvironment and highlight emerging therapeutic strategies for targeting mechanical forces in immunotherapy.

关键词： mechanical force microenvironment immunotherapy extracellular matrix cancer

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The RLCK-VND6 module coordinates secondary cell wall formation and adaptive growth in rice

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Molecular Plant 2023年第6期16卷 999-1015页

作者： Shaoxue Cao Yan Wang Yihong Gao Rui Xu Jianing Ma Zuopeng Xu Keke Shang-Guan Baocai Zhang Yihua Zhou State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyThe Innovative Academy of Seed DesignChinese Academy of SciencesBeijing100101China University of Chinese Academy of Sciences Beijing 100049China jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of the Ministry of Education for Plant Functional Genomics College of AgricultureYangzhouUniversityYangzhou225009China

The orderly deposition of secondary cell wall(SCW)in plants is implicated in various biological programs and is precisely controlled.Although many positive and negative regulators of SCW have been documented,the molecular mechanisms underlying SCW formation coordinated with distinct cellular physiological processes during plant adaptive growth remain largely unclear.Here,we report the identification of Cellulose Synthase co-expressed Kinase1(CSK1),which encodes a receptor-like cytoplasmic kinase,as a negative regulator of SCW formation and its signaling cascade in rice.Transcriptome deep sequencing of developing internodes and genome-wide co-expression assays revealed that CSK1 is co-expressed with cellulose synthase genes and is responsive to various stress stimuli.The increased SCW thickness and vigorous vessel transport in csk1 indicate that CSK1 functions as a negative regulator of SCW biosynthesis.Through observation of green fluorescent protein-tagged CSK1 in rice protoplasts and stable transgenic plants,we found that CSK1 is localized in the nucleus and cytoplasm adjacent to the plasma membrane.Biochemical and molecular assays demonstrated that CSK1 phosphorylates VASCULAR-RELATED NAC-DOMAIN 6(VND6),a master SCW-associated transcription factor,in the nucleus,which reduces the transcription of a suite of SCW-related genes,thereby attenuating SCW accumulation.Consistently,genetic analyses show that CSK1 functions upstream of VND6 in regulating SCW formation.Interestingly,our physiological analyses revealed that CSK1 and VND6 are involved in abscisic acid-mediated regulation of cell growth and SCW deposition.Taken together,these results indicate that the CSK1-VND6 module is an important component of the SCW biosynthesis machinery,which coordinates SCW accumulation and adaptive growth in rice.Our study not only identifies a new regulator of SCW biosynthesis but also reveals a fine-tuned mechanism for precise control of SCW deposition,offering tools for rationally tailoring agronomic traits.

关键词： RLCK signaling kinase fine-tuning regulator secondary cell wall biosynthesis mechanical force ABA adaptation

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Distortion of frontal bones results from cell apoptosis induced by the mechanical force from the migrating eye during metamorphosis in Paralichthys olivaceus

Distortion of frontal bones results from cell apoptosis indu...

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第二届全国发育生物学大会

作者： Mingyan Sun Baolong Bao Shanghai Ocean University

The left/right asymmetry of adult flatfishes(Pleuronectiformes)is remarkable given the external body symmetry of the larval fish.The asymmetry of the cranium mainly results from distortion of the frontals during metamorphosis in flatfishes.Recently,it is showed that distortion of frontal bones is caused by the migrated eye.However,the molecular mechanism on regulating the distortion of the frontals is unclear.In this study,we first show that distortion of frontal bones during metamorphosis in Paralichthys olivaceus is the result of cell apoptosis,rather than cell autophage or cell proliferation.Secondly,we found that the cell apoptosis in frontals is induced by the mechanical force transferred from the eye upward migration in P.olivaceus.mechanical force signal from the up-migrating eye transmit from FAK to downstream gene and are integrated into the BMP-2 signal pathway.Finally,it is clear that the cell apoptosis in frontal bones is activated by the intrinsic mitochondrial pathway under FAK-mediating.The extrinsic death receptor should not be involved in the process.In addition,the cell apoptosis in frontal bones in P.olivaceus was not induced directly by thyroid hormone,which is regarded as to mediate cell apoptosis directly during amphibian metamorphosis.These findings help us to outline the major signaling route regulating the distortion of frontals during metamorphosis in flatfishes.Meanwhile,they tell us that the asymmetry of the cranium,at least the distortion of frontals,is rather the result than the reason of eye migration.These finding is important to better understand the evolution of flatfishes.

关键词： flatfishes frontal bones cell apoptosis mechanical force eye migration metamorphosis

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Understanding cell-extracellular matrix interactions for topology-guided tissue regeneration

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BIOCELL 2023年第4期47卷 789-808页

作者： AAYUSHI RANDHAWA SAYAN DEB DUTTA KEYA GANGULY TEJAL V.PATIL RACHMI LUTHFIKASARI KI-TAEK LIM Department of Biosystems Engineering Kangwon National UniversityChuncheon24341Korea Interdisciplinary Program in Smart Agriculture Kangwon National UniversityChuncheon24341Korea

Tissues are made up of cells and the extracellular matrix(ECM)which surrounds them.These cells and tissues are actively adaptable to enduring significant stress that occurs in daily life.This astonishing mechanical stress develops due to the interaction between the live cells and the non-living ECM.Cells in the matrix microenvironment can sense the signals and forces produced and initiate a signaling cascade that plays a crucial role in the body’s normal functioning and influences various properties of the native cells,including growth,proliferation,and differentiation.However,the matrix’s characteristic features also impact the repair and regeneration of the damaged tissues.The current study reviewed how the cell-ECM interaction regulates cellular behavior and physicochemical properties.Herein,we have described the response of cells to mechanical stresses,the importance of substrate stiffness and geometry in tissue regeneration,and the development of scaffolds to mimic the nature of native ECM in 3D for tissue engineering applications has also been discussed.Finally,the study summarizes the conclusions and promising prospects based on the cell-ECM interplay.

关键词： ECM Stress mechanical force Scaffold Mechanotransduction Tissue engineering

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Organoboron luminophores with extremely strong dual-phase emissions

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Chinese Chemical Letters 2023年第3期34卷 274-278页

作者： Qingsong Liu Man Zhang Ye Fu Shen Shen Liangliang Zhu Department of Macromolecular Science State Key Laboratory of Molecular Engineering of PolymersFudan UniversityShanghai 200438China

Developing efficient dual-phase emission emitters upon organoboron luminophores remains a formidable challenge due to the ubiquitous self-absorption and deleterious π-π interactions from aromatic structure.Here, a new family of benzothiazole-enolate-based organoboron luminophores(HN1-4) with effective dual-phase emission was constructed. HN4 showed almost the highest quantum yield(QY) among this type of compound so far. The three-ring-fused rigid skeleton and moderate intramolecular charge transfer(ICT) effect ensured that HN4 could give rise to extremely strong emission in any solution(QY up to 99%). X-ray crystallographic analysis showed that the twisted core structure constructed by the boronic coordination of two penta-fluorobenzene of HN4 was responsible for intense emission in the solid state(QY up to 68%). Besides, HN4 exhibited a unique response to mechanical force accompanied by a reversible change of the QY. We believe that this strategy provides beneficial inspiration and methodology to design materials with high emissive quantum yield that can be used in a variety of luminescent events.

关键词： Organoboron Dual-phase emission Quantum yield Rigid-twisted core mechanical force

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Toward an optimized strategy of using various airway mucus clearance techniques to treat critically ill COVID-19 patients

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BIOCELL 2022年第4期46卷 855-871页

作者： MINGZHI LUO KAI NI YAN SUN JIA GUO KANG WEN LINHONG DENG Changzhou Key Laboratory of Respiratory Medical Engineering Institute of Biomedical Engineering and Health SciencesChangzhou UniversityChangzhou213164China

Coronavirus disease 2019(COVID-19)caused by acute respiratory syndrome coronavirus 2(SARS-Cov-2)is still threatening the human life and society throughout the world.For those critically ill patients,mechanical ventilation(MV)is essential to provide life support during treatment.However,both the virus infection and MV disrupt the balance between secretion and elimination of airway mucus and lead to mucus accumulation in the lung.Postmortem examination verified that the lungs in patients died of COVID-19 are indeed filled with sticky mucus,suggesting a great need to improve airway mucus clearance in critically ill COVID-19 patients.Therefore,it may be helpful to comprehensively review the current understanding regarding the changes of biochemical and rheological features of airway mucus associated with the disease,as well as the physiological principles and algorithm to decide airway clearance techniques suitable for the critically ill COVID-19 patients.Based on these considerations,optimized strategies may be developed to eliminate the airway mucus accumulated in the airways of critically ill COVID-19 patients.

关键词： COVID-19 Airway mucus Rheology mechanical force Airway clearance techniques

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Role of the mechanical microenvironment in cancer development and progression

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Cancer Biology & Medicine 2020年第2期17卷 282-292页

作者： Qiuping Liu Qing Luo Yang Ju Guanbin Song Key Laboratory of Biorheological Science and Technology Ministry of EducationCollege of BioengineeringChongqing UniversityChongqing 400030China Department of mechanical Science and Engineering Nagoya UniversityNagoya 464-8603Japan

Cross-talk between tumor cells and mechanical stress in the tumor microenvironment has been shown to be involved in carcinogenesis.High mechanical stress in tumors can alter the metabolism and behaviors of cancer cells and cause cancer cells to attain cancer stem-like cell properties,thus driving tumor progression and promoting metastasis.The mechanical signal is converted into a biochemical signal that activates tumorigenic signaling pathways through mechanotransduction.Herein,we describe the physical changes occurring during reprogramming of cancer cell metabolism,which regulate cancer stem cell functions and promote tumor progression and aggression.Furthermore,we highlight emerging therapeutic strategies targeting mechanotransduction signaling pathways.

关键词： Cancer stem cell cell metabolism mechanical force tumor progression

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Mechanotransduction,nanotechnology,and nanomedicine

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The Journal of Biomedical Research 2021年第4期35卷 284-293页

作者： Xiaowei Liu Fumihiko Nakamura School of Pharmaceutical Science and Technology Tianjin UniversityTianjin 300072China.

Mechanotransduction,a conversion of mechanical forces into biochemical signals,is essential for human development and physiology.It is observable at all levels ranging from the whole body,organs,tissues,organelles down to molecules.Dysregulation results in various diseases such as muscular dystrophies,hypertension-induced vascular and cardiac hypertrophy,altered bone repair and cell deaths.Since mechanotransduction occurs at nanoscale,nanosciences and applied nanotechnology are powerful for studying molecular mechanisms and pathways of mechanotransduction.Atomic force microscopy,magnetic and optical tweezers are commonly used for force measurement and manipulation at the single molecular level.force is also used to control cells,topographically and mechanically by specific types of nano materials for tissue engineering.Mechanotransduction research will become increasingly important as a sub-discipline under nanomedicine.Here we review nanotechnology approaches using force measurements and manipulations at the molecular and cellular levels during mechanotransduction,which has been increasingly play important role in the advancement of nanomedicine.

关键词： nanomedicine mechanotransduction mechanical force differentiation tissue engineering

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Nanoscale domain switching mechanism of Bi_(3.15)Eu_(0.85)Ti_3O_(12) thin film under the different mechanical forces

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Chinese Physics B 2015年第10期24卷 496-500页

作者：朱哲陈玉博郑学军 School of Materials Science and Engineering Xiangtan University School of mechanical Engineering Xiangtan University

The switching process of ferroelectric thin films in electronic devices is one of the most important requirements for their application. Especially for the different external fields acting on the film surface, the mechanism of domain switching is more complicated. Here we observe the nanoscale domain switchings of Bi3.15Eu0.85Ti3O12 thin film under different mechanical forces at a fast scan rate. As the force increases from initial state to 247.5 n N, the original bright or grey contrasts within the selected grains are all changed into dark contrasts corresponding to the polarization vectors reversed from the up state to the down state, except for the clusters. As the mechanical force increases to 495 n N, the color contrasts in all of the selected grains further turn into grey contrasts and some are even changed into grey contrasts completely showing the typical 90° domain switching. When another stronger loading force 742.5 n N is applied, the phase image becomes unclear and it indicates that the piezoelectric signal can be suppressed under a sufficiently high force, which is coincident with previous experimental results. Furthermore, we adopt the domain switching criterion from the perspective of equilibrium state free energy of ferroelectric nanodomain to explain the mechanisms of force-generated domain switchings.

关键词： thin film domain switching mechanical force piezoresponse force microscopy

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