检索结果-南通市图书馆

Identification of New Genes Related to Virulence of <i>Xanthomonas axonopodis</i>Pv. <i>Citri</i>during Citrus Host Interactions

引用

Advances in Microbiology 2017年第1期7卷 22-46页

作者： Cristiano B. Ferreira Leandro M. Moreira Joice B. Brigati Lonjoré L. Lima Jesus A. Ferro Maria I. T. Ferro Julio C. F. de Oliveira Department of Technology Faculty of Agricultural Sciences and Veterinary S&atilde o Paulo State University (UNESP) Jaboticabal SP Brazil Citriculture Research Center Sylvio Moreira (IAC-Campinas) SP Brazil Department of Biological Sciences Institute of Exact and Biological Sciences Federal University of Ouro Preto Ouro Preto MG Brazil Research Center in Biological Sciences (NUPEB) Department of Biological Sciences Institute of Exact and Biological Sciences Federal University of Ouro Preto Ouro Preto MG Brazil Plant Healthy Department Faculty of Agricultural Sciences and Veterinary S&atilde o Paulo State University (UNESP) Jaboticabal SP Brazil Department of Biological Sciences Federal University of S&atilde o Paulo (UNIFESP) Diadema SP Brazil

A mutant library of the bacterium Xanthomonas citri subsp. citri strain 306 pathotype A (Xac), the causative agent of most aggressive Asiatic type A citrus canker, was screened regarding altered canker symptoms after inoculations into Citrus sinensis and Citrus limonia host leaves. Twenty-six mutants have shown phenotypic virulence changes and have respectively knocked out gene identified by sequencing. In vivo growth curves were obtained for nine mutants to quantify how the mutations could affect pathogen’s adaptability to growth inside and attack host plant infected tissue. Among identified genes in mutated strains, we could find those that until now had not been reported as being involved in Xac adaptation and/or virulence, such as predicted to encode for xylose repressor-like protein (XACΔxylR), Fe-S oxidoredutase (XACΔaslB), helicase IV (XACΔhelD), ubiquinol cytochrome c oxidoreductase iron-sulfur subunit (XACΔpetA), chromosome partitioning protein (XACΔparB) and cell division protein FtsB (XACΔftsB), in addition to genes predicted to encode for hypothetical proteins. The new genes found in this study as being relevant to adaptation and virulence, improve the understanding of Xac fitness during citrus plant attack and canker symptoms development.

关键词： Xanthomonas axonopodis Pv. Citri mutants Impaired Virulence

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Hepatocellular carcinoma and hepatitis B surface protein

引用

World Journal of Gastroenterology 2016年第6期22卷 1943-1952页

作者： Yong-Wei Li Feng-Cai Yang Hui-Qiong Lu Jiong-Shan Zhang Department of Traditional Chinese Medicine the Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510630Guangdong ProvinceChina Department of Medical Record Room the Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510630Guangdong ProvinceChina Medical Research Center the Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510630Guangdong ProvinceChina

The tumorigenesis of hepatitis B virus(HBV)-associated hepatocellular carcinoma(HCC) has been widely studied. HBV envelope proteins are important for the structure and life cycle of HBV, and these proteins are useful for judging the natural disease course and guiding treatment. Truncated and mutated pre S/S are produced by integrated viral sequences that are defective for replication. The pre S/S mutants are considered "precursor lesions" of HCC. Different pre S/S mutants induce various mechanisms of tumorigenesis, such as transactivation of transcription factors and an immune inflammatory response, thereby contributing to HCC. The pre S2 mutants and type Ⅱ "Ground Glass" hepatocytes represent novel biomarkers of HBVassociated HCC. The pre S mutants may induce the unfolded protein response and endoplasmic reticulum stress-dependent and stress-independent pathways. Treatments to inhibit hepatitis B surface antigen(HBs Ag) and damage secondary to HBs Ag or the pre S/S mutants include antivirals and antioxidants, such as silymarin, resveratrol, and glycyrrhizin acid. Methods for the prevention and treatment of HCC should be comprehensive.

关键词： Hepatitis B surface protein Hepatocellularcarcinoma PreS/S mutants Endoplasmic reticulumstress "Ground Glass" hepatocytes

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Using Forward Genetic Approach to Understand Cell Wall Biosynthesis and Its Biological Functions

Using Forward Genetic Approach to Understand Cell Wall Biosy...

引用

2011全国植物生物学研讨会

作者： Baocai Zhang,Rui Li,Lifeng Liu,Xiangling Liu and Yihua Zhou~* State Key Laboratory of Plant Genomics and National Center for Plant Gene Research,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China

Cell wall is a basic cellular structure of plant *** plays many fundamental roles in plant growth and development,including providing mechanical support, determining cell shape,defending against biotic and abiotic stresses,functioning as signal molecules,and transporting water and nutrient and mineral *** genetic approach is a powerful way for uncovering the secrets in cell wall biosynthesis and its roles in plant *** group focuses on systematical isolation of mutants that have altered mechanical strength and/or plant height,as well as other

关键词： cell wall biosynthesis forward genetic approach mutants plant growth

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Genetic Dissection of the Cytokinin Signaling Pathway in Arabidopsis thaliana

Genetic Dissection of the Cytokinin Signaling Pathway in Ara...

引用

中国细胞生物学学会第八届会员代表大会暨学术大会

作者： Jiaqiang Sun, Binglian Zheng, Suzhi Zhang and Jianru Zuo Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 917 Datun Road, Beijing 100101, China (Contributed equally to this work

Cytokinin is a key growth regulator implicatcd in many aspccts of plantgrowth and development, including the control of the apical dominance, rootgrowth, vascular tissuc development, nowering time and leaf senescence. Recentstudies suggested that cytokinin signal transduction may employ a mechanismsimilar to that of the prokaryotic two-component system. However, themolecular mechanism of the cytokinin signaling remains largely unknown. Wehave previously identified two gain-of-function mutations pga1 and pga22, whichcaused typical cytokinin response in thc absence of the hormone. Molecular

关键词： cytokinin mutants Arabidopsis suppressors of ckil suppressor of ipt8

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Ethylene signaling in rice-some novel insights

Ethylene signaling in rice-some novel insights

引用

2018全国植物生物学大会

作者： He Zhao Kai-Xuan Duan Hui Chen Cui-Cui Yin Biao Ma Wan-Ke Zhang Shou-Yi Chen Jin-Song Zhang State Key Laboratory of Plant Genomics Institute of Genetics & Developmental BiologyChinese Academy of Sciences

Rice is an important monocotyledonous crop worldwide and lives in water environment in most of its life *** as a gas phytohormone plays significant roles in the adaptation of rice to the water ***,the understanding of ethylene signaling in rice is very *** a distinct phenotype of root inhibition but coleoptile promotion in rice etiolated seedlings upon ethylene treatment,we have isolated a set of rice ethylene-response mutants from T-DNA insertion and EMSmutagenized populations,and identified the corresponding genes through map-based *** these,MHZ7/OsEIN2 and MHZ6/OsEIL1 are similar to those in ***6/OsEILl and OsEIL2 are responsible for the ethylene responses in roots and coleoptiles *** of these genes caused salt tolerance,different from the observation in *** also identified MHZ5/carotenoid isomerase and MHZ4/ABA4,both of which are involved in ABA biosynthesis,supporting that ethylene signaling acts upstream of ABA pathway to regulate root growth in *** characterization of a gaoyaol(gy1) mutant exhibiting longer mesocotyl and longer coleoptile compared to its original variety,the GY1 gene was identified to encode a PLA1-type *** GY1 functions at the initial step of jasmonic acid(JA) *** inhibits expression of GY1 and other genes in the JA biosynthesis pathway to reduce JA levels and enhance the growth of mesocotyl and coleoptile through promotion of cell *** analysis of the re-sequencing data from 3000 rice accessions,we identified a single natural variation of the GY1 gene,GY1,which contributes to mesocotyl elongation in rice varieties and is beneficial for emergence of seedlings in dry land after direct ***3 is a novel membrane protein localized on *** can stabilize the OsEIN2 through association with the transmembrane domain of OsEIN2,strengthening the importance of the OsEIN2 transmembrane domain during ethyle

关键词： Ethylene rice mutants signaling stress

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Genetic basis of meiotic abnormalities in non-obstructive azoospermic patients

Genetic basis of meiotic abnormalities in non-obstructive az...

引用

2017中国长三角遗传学大会

作者： Qinghua Shi School of Life Sciences University of Science and Technology of China

Meiosis,which is essential for gametogenesis of sexual reproduction,is characterized by recognition,pairing,synapsis,recombination and segregation of homologous *** sequential occurrence of these chromosomal events during meiosis is dependent on the generation of programmed DNA double-strand breaks(DSBs) followed by repair using the homologous chromosome as a *** mistakes occurred in either steps will lead to spermatogenic failure,thus inducing male *** genomic studies have shown that deletion of each of more than 100 genes in mice can lead to abnormal meiosis,but so far mutations in about 10 genes known to be responsible for meiotic abnormalities in infertile human *** reveal the molecular basis underlying human male infertility caused by abnormal meiosis,whole exome sequencing was performed on patients with unexplained male infertility caused by abnormal meiosis,followed by functional verification in mouse models carrying the human mutants.A number of mutations of genes with or without known function in meiosis were *** results not only reveal the pathological causes underlying meiotic abnormalities in human azoospermic patients,but also provide a new strategy for the discovery of novel meiotic regulators.

关键词： Non-obstructive azoospermia Spermatogenesis Meiosis mutants

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

玉米大斑病菌NPS6基因的克隆和功能分析

玉米大斑病菌NPS6基因的克隆和功能分析

引用

作者：阿里吉林大学

学位级别：硕士

Maize is the important cereal crop in the world after wheat and rice. Many countries in the worlduse maize for different purposes due to its economic important value domestically and *** production is faced with many constraints in the field and post harvest. The major constrainthindering high production is Northern Corn Leaf Blight disease caused by the fungus Setosphaeriaturcica which is a member of ascomycetes filamentous fungi. In this group of fungi, the virulencecharacteristics is determined by NPS6gene which play roles in preventing Hydrogen peroxideproduced by the maize plants. However, NPS6gene in Setosphaeria turcica as not yet been cloneddespite the fact that NPS6gene is conserved throughout filamentous fungi.Therefore, in this study we aim at cloning and functionally characterize the NPS6gene inSetosphaeria turcica.Different experiments were conducted in such a way that this gene can be cloned and functionalcharacterized.Gene length9420bp was first cloned by applying a genome walking method and technique. Thegene information was available relying other filamentous fungi such as Magnaporthe oryzae,Cochliobolus heterostrophus and Neurospora crassa N PS6.Knockout vector pXEH-NPS6LRF was constructed by1350bp sequence for the left flank and1000bp for the right flank separated by Hygromycin phosphotranferase (hph) insert under trpcpromoter. Protoplast transformation method was used where by Fungal strain St28A was preparedthe protoplasts and used in the transformation with vector for regeneration of *** for the mutants was performed by analyzing all collected transformants for the presenceof hph. The analysis revealed that, all transformants consisted the hph gene, not only that, they werealso analyzed for the presence of NPS6gene. We got some transformants right now. We are doingvalidation for the transformants looking for the real NPS6knockout mutants.

关键词： Cloning Setosphaeria turcica Protoplasts transformation mutants wild type.

来源：

同方学位论文库评论

在线全文

同方学位论文库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：