Identification of New Genes Related to Virulence of <i>Xanthomonas axonopodis</i>Pv. <i>Citri</i>during Citrus Host Interactions

作     者：Cristiano B. Ferreira Leandro M. Moreira Joice B. Brigati Lonjoré L. Lima Jesus A. Ferro Maria I. T. Ferro Julio C. F. de Oliveira 

作者机构：Department of Technology Faculty of Agricultural Sciences and Veterinary Sã o Paulo State University (UNESP) Jaboticabal SP Brazil Citriculture Research Center Sylvio Moreira (IAC-Campinas) SP Brazil Department of Biological Sciences Institute of Exact and Biological Sciences Federal University of Ouro Preto Ouro Preto MG Brazil Research Center in Biological Sciences (NUPEB) Department of Biological Sciences Institute of Exact and Biological Sciences Federal University of Ouro Preto Ouro Preto MG Brazil Plant Healthy Department Faculty of Agricultural Sciences and Veterinary Sã o Paulo State University (UNESP) Jaboticabal SP Brazil Department of Biological Sciences Federal University of Sã o Paulo (UNIFESP) Diadema SP Brazil 

出 版 物：《Advances in Microbiology》 (微生物学（英文）)

年 卷 期：2017年第7卷第1期

页      面：22-46页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Xanthomonas axonopodis Pv. Citri Mutants Impaired Virulence 

摘      要：A mutant library of the bacterium Xanthomonas citri subsp. citri strain 306 pathotype A (Xac), the causative agent of most aggressive Asiatic type A citrus canker, was screened regarding altered canker symptoms after inoculations into Citrus sinensis and Citrus limonia host leaves. Twenty-six mutants have shown phenotypic virulence changes and have respectively knocked out gene identified by sequencing. In vivo growth curves were obtained for nine mutants to quantify how the mutations could affect pathogen’s adaptability to growth inside and attack host plant infected tissue. Among identified genes in mutated strains, we could find those that until now had not been reported as being involved in Xac adaptation and/or virulence, such as predicted to encode for xylose repressor-like protein (XACΔxylR), Fe-S oxidoredutase (XACΔaslB), helicase IV (XACΔhelD), ubiquinol cytochrome c oxidoreductase iron-sulfur subunit (XACΔpetA), chromosome partitioning protein (XACΔparB) and cell division protein FtsB (XACΔftsB), in addition to genes predicted to encode for hypothetical proteins. The new genes found in this study as being relevant to adaptation and virulence, improve the understanding of Xac fitness during citrus plant attack and canker symptoms development.