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Evaluation of the chemical profile from four germplasms sources of Pruni Semen using UHPLC-LTQ-Orbitrap-MS and multivariate analyses

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Journal of Pharmaceutical Analysis 2022年第5期12卷 733-742页

作者： Zihan Zhao Yue Liu Yushi Zhang Zeyu Geng Rina Su Lipeng Zhou Chao Han Zhanjun Wang Shuangcheng Ma Weidong Li School of Chinese Materia Medica Beijing University of Chinese MedicineBeijing102488China Institute of Forestry and Grassland Ecology Ningxia Academy of Agricultural and Forestry SciencesYinchuan750002China Institute for Control of Chinese Traditional Medicine and Ethnic Medicine National Institutes for Food and Drug ControlBeijing100050China

Pruni Semen,the seed of several unique Prunus plants,is a traditional purgative herbal *** determine the authentic sources of Pruni Semen,46 samples from four species were collected and *** compounds including multiflorin A(Mul A),a notable purative compound,were isolated and identified by chemical separation and nuclear magnetic resonance ***-six communal components were identified by ultra-high performance liquid chromatography with linear ion trap-quadrupole Orbitrap mass spectrometry,and acetyl flavonoid glycosides were recognized as characteristic *** flavonoids were distributed in the seed coat and cyanogenic glycosides in the *** on this,methods for identifying Pruni Semen from different sources were established using chemical fingerprinting,quantitative analysis of the eight principal compounds,hierarchical cluster analysis,principal component analysis,and orthogonal partial least squares discriminant *** results showed that the samples were divided into two categories:one is the small seeds from Prunus humilis(Ph)and Prunus japonica(Pj),and the other is the big seeds from Prunus pedunculata(Pp)and Prunus triloba(Pt).The average content of Mul A was 3.02,6.93,0.40,and 0.29 mg/g,while the average content of amygdalin was 18.5,17.7,31.5,and 30.9 mg/g in Ph,Pj,Pp,and Pt,*** the above information suggests that small seeds might be superior sources of Pruni *** is the first comprehensive report on the identification of chemical components in Pruni Semen from different species.

关键词： Pruni semen Acetyl flavonoids Amygdalin multivariate analyses Authentic sources

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Overstory functional groups indicate the legacy of land use in a secondary tropical forest in southwestern China

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JOURNAL OF FORESTRY RESEARCH 2024年第1期35卷 1-15页

作者： Deng, Yun Zhang, Wenfu Cao, Min Dong, Jinlong Chen, Hui Deng, Xiaobao Liu, Jiajia Song, Xiaoyang Xia, Shangwen Sha, Liqing Yuan, Shengdong Lin, Luxiang Chinese Acad Sci CAS Key Lab Trop Forest Ecol Xishuangbanna Trop Bot Garden Mengla 666303 Peoples R China Chinese Acad Sci Ctr Plant Ecol Core Bot Gardens Mengla 666303 Peoples R China Chinese Acad Sci Natl Forest Ecosyst Res Stn Xishuangbanna Xishuangbanna Trop Bot Garden Mengla 666303 Peoples R China Univ Chinese Acad Sci Dept Life Sci Beijing 100049 Peoples R China Fudan Univ Dept Life Sci Shanghai 570228 Peoples R China

Anthropogenic disturbances are widespread in tropical forests and influence the species composition in the overstory. However, the impacts of historical disturbance on tropical forest overstory recovery are unclear due to a lack of disturbance data, and previous studies have focused on understory species. In this study, the purpose was to determine the influence of historical disturbance on the diversity, composition and regeneration of overstory species in present forests. In the 20-ha Xishuangbanna tropical seasonal rainforest dynamics plot in southwestern China, the historical disturbance boundaries were delineated based on panchromatic photographs from 1965. Factors that drove species clustering in the overstory layer (DBH >= 40 cm) were analyzed and the abundance, richness and composition of these species were compared among different tree groups based on multiple regression tree analysis. The coefficient of variation of the brightness value in historical panchromatic photographs from 1965 was the primary driver of species clustering in the overstory layer. The abundance and richness of overstory species throughout the regeneration process were similar, but species composition was always different. Although the proportion of large-seeded and vigorous-sprouting species showed no significant difference between disturbed and undisturbed forests in the treelet layer (DBH < 20 cm), the difference became significant when DBH increased. The findings highlight that historical disturbances have strong legacy effects on functional group composition in the overstory and the recovery of overstory species was multidimensional. Functional group composition can better indicate the dynamics of overstory species replacement during secondary succession.

关键词： Historical disturbance Legacy effects Secondary forests Overstory species Functional groups RAIN-FOREST multivariate analyses SEED SIZE VEGETATION TREES XISHUANGBANNA REGENERATION DISTURBANCE RECOVERY YUNNAN

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Weathering and accumulation of trace elements in the soils of the Porali Plain, Balochistan: repercussions in agriculture

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Acta Geochimica 2024年第2期43卷 214-234页

作者： Maria Kaleem Erum Bashir Shahid Naseem Tahir Rafique Bushra Shahab Department of Geology University of KarachiKarachi 75270Pakistan Applied Chemistry Research Centre PCSIR Laboratories ComplexKarachi 75280Pakistan

This study is theﬁrst attempt to assess the nature of the soil,especially on the western side of the Porali Plain in Balochistan;a new emerging agriculture hub,using weathering and pollution indices supplemented by multi-variate analysis based on geochemical *** outcomes of this study are expected to help farmers in soil manage-ment and selecting suitable crops for the ***-ﬁve soil samples were collected,mainly from the arable land of the Porali *** drying and coning-quarter-ing,soil samples were analyzed for major and trace ele-ments using the XRF technique;sieving and hydrometric methods were employed for granulometric ***-mated data were analyzed using Excel,SPSS,and Surfer software to calculate various indices,correlation matrix,and spatial *** granulometric analysis showed that 76%of the samples belonged to loam types of soil,12%to sand type,and 8%to silt *** indices:CIA,CIW,PIA,PWI,WIP,CIX,and ICV were calculated to infer the level of *** indices reﬂect mod-erate to intense weathering;supported by K_(2)O/AI_(2)O_(3),Rb/K_(2)O,Rb/Ti,and Rb/Sr *** of the geo-ac-cumulation and Nemerow Pollution indices pinpoint rela-tively high concentrations of Pb,Ni,and Cr concentration in the *** correlation matrix and Principal Compo-nent Analysis show that the soil in this study area is mainly derived from the weathering of igneous rocks of Bela Ophiolite(Cretaceous age)and Jurassic sedimentary rocks of Mor Range having SEDEX/MVT type *** may result in the undesirable accumulation of certain trace elements which adversely affects crops.

关键词： Weathering indices Pollution indices Accumulation Repercussions Trace elements multivariate analyses Porali Plain Balochistan

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Metabolic Profiling of Human Colorectal Cancer Using High Resolution 1H Nuclear Magnetic Resonance Spectroscopy

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Chinese Journal of Chemistry 2011年第11期29卷 2511-2519页

作者：陈文学周晓燕黄丹陈芬儿杜祥 Department of Chemistry Fudan University Shanghai 200433 China Department of Pathology and Oncology Cancer Hospital Fudan University Shanghai 200032 China

Colorectal cancer （CRC） is the third commonest malignancy cancer worldwide. Clear understandings of global metabolic profiling of the normal mucosa and cancer tissues are vitally important to aid optimizing the clinical management strategy and understanding CRC biology. We studied metabolic characteristics of 20 CRC and 20 distant normal mucosa tissues extracts from 20 patients using high resolution ^1H NMR spectroscopy in conjunction with multivariate analyses, such as principal component analysis （PCA）. Compared with distant normal mucosa tissues, lactate, taurine, ornithine and polyamine were present at significantly higher levels in CRC tissue extracts whereas myo-inositol was present at significantly lower level. Two metabolites ratios such as myo-inositolltaurine and myo-inositol/（ornithine＋polyamine） appear to be the most valuable biomarkers for the differentiation CRC from normal mucosa tissues. Our data suggested that HR ~H NMR spectroscopy combined with multivariate analy- ses is a potentially useful technology for detecting malignant changes in the normal mucosa tissues, the technique may be further exploited for future CRC biomarker research or identification of targets for therapeutic manipulations.

关键词： colorectal cancer NMR spectroscopy metabolic profiling multivariate analyses principal component analysis (PCA) orthogonal partial least squares-discriminant analysis (OPLS-DA)

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