Evaluation of the chemical profile from four germplasms sources of Pruni Semen using UHPLC-LTQ-Orbitrap-MS and multivariate analyses

作     者：Zihan Zhao Yue Liu Yushi Zhang Zeyu Geng Rina Su Lipeng Zhou Chao Han Zhanjun Wang Shuangcheng Ma Weidong Li Zihan Zhao;Yue Liu;Yushi Zhang;Zeyu Geng;Rina Su;Lipeng Zhou;Chao Han;Zhanjun Wang;Shuangcheng Ma;Weidong Li

作者机构：School of Chinese Materia MedicaBeijing University of Chinese MedicineBeijing102488China Institute of Forestry and Grassland EcologyNingxia Academy of Agricultural and Forestry SciencesYinchuan750002China Institute for Control of Chinese Traditional Medicine and Ethnic MedicineNational Institutes for Food and Drug ControlBeijing100050China 

出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)

年 卷 期：2022年第12卷第5期

页      面：733-742页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100701[医学-药物化学] 10[医学] 

基　　金：sponsored by the Key Research and Development Programs in the Ningxia Hui Autonomous Region,China(Grant No.:2020BBF02027) Beijing Natural Science Foundation(Grant No.:5212014) 

主　　题：Pruni semen Acetyl flavonoids Amygdalin Multivariate analyses Authentic sources 

摘      要：Pruni Semen,the seed of several unique Prunus plants,is a traditional purgative herbal *** determine the authentic sources of Pruni Semen,46 samples from four species were collected and *** compounds including multiflorin A(Mul A),a notable purative compound,were isolated and identified by chemical separation and nuclear magnetic resonance ***-six communal components were identified by ultra-high performance liquid chromatography with linear ion trap-quadrupole Orbitrap mass spectrometry,and acetyl flavonoid glycosides were recognized as characteristic *** flavonoids were distributed in the seed coat and cyanogenic glycosides in the *** on this,methods for identifying Pruni Semen from different sources were established using chemical fingerprinting,quantitative analysis of the eight principal compounds,hierarchical cluster analysis,principal component analysis,and orthogonal partial least squares discriminant *** results showed that the samples were divided into two categories:one is the small seeds from Prunus humilis(Ph)and Prunus japonica(Pj),and the other is the big seeds from Prunus pedunculata(Pp)and Prunus triloba(Pt).The average content of Mul A was 3.02,6.93,0.40,and 0.29 mg/g,while the average content of amygdalin was 18.5,17.7,31.5,and 30.9 mg/g in Ph,Pj,Pp,and Pt,*** the above information suggests that small seeds might be superior sources of Pruni *** is the first comprehensive report on the identification of chemical components in Pruni Semen from different species.