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Monilinia fructicola on loquat:An old pathogen invading a new host

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Journal of Integrative Agriculture 2021年第7期20卷 2009-2014页

作者： YIN Liang-fen ZHANG Shu-qin DU Juan WANG Xin-yu XU Wen-xing LUO Chao-xi Key Lab of Horticultural Plant Biology Ministry of Education/Huazhong Agricultural UniversityWuhan 430070 P.R.China Experimental Teaching Center of Crop Science College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhan 430070P.R.China Key Laboratory of Crop Disease Monitoring and Safety Control in Hubei Province College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhan 430070P.R.China Key Laboratory of Oasis Agricultural Pest Management and Plant Protection Resources Utilization College of AgricultureShihezi 832003P.R.China

Monilinia fructicola has been widely reported as the causal agent of brown rot disease on many Rosaceae family fruits *** has been reported on stone fruits,e.g.,peach,plum,cherry,apricot and mume;as well as pome fruits,e.g.,apple,pear and *** is a member of the Eriobotrya genus in subfamily Maloideae along with apple,pear and *** far,loquat has not been reported as the host of any Monilia *** June 2019,brown rot symptoms were observed on loquat fruits in an orchard in Wuhan,Hubei Province,*** single spore isolates were obtained and identified as *** based on morphological characteristics and molecular *** is the first report of loquat brown rot disease caused by Monilia species in the ***,upon artificial inoculation,all three Monilia species from peach in China,i.e.,***,*** and ***,could cause typical brown rot disease on loquat *** the same time,*** isolates from loquat showed virulence similar to those isolates from peach when the pathogenicity test was conducted on peach *** results suggested that loquat could be infected by other Monilia species and that isolates from loquat also have potential to damage other Rosaceae family fruits in practice.

关键词： Monilia species brown rot loquat Monilinia fructicola

Sorbitol induces flower bud formation via the MADS-box transcription factor EjCAL in loquat

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Journal of Integrative Plant Biology 2023年第5期65卷 1241-1261页

作者： Hong-Xia Xu Dong Meng Qing Yang Ting Chen Meng Qi Xiao-Ying Li Hang Ge Jun-Wei Chen Institute of Horticulture Zhejiang Academy of Agricultural SciencesHangzhou 310021China Beijing Advanced Innovation Center for Tree Breeding by Molecular Design Beijing Forestry UniversityBeijing 100000China College of Forestry Beijing Forestry UniversityBeijing 100083China

Sorbitol is an important signaling molecule in fruit trees. Here, we observed that sorbitol increased during flower bud differentiation(FBD) in loquat(Eriobotrya japonica Lindl.). Transcriptomic analysis suggested that bud formation was associated with the expression of the MADS-box transcription factor(TF) family gene, EjCAL. RNA fluorescence in situ hybridization showed that EjCAL was enriched in flower primordia but hardly detected in the shoot apical meristem. Heterologous expression of EjCAL in Nicotiana benthamiana plants resulted in early FBD. Yeast-one-hybrid analysis identified the ERF12 TF as a binding partner of the EjCAL promoter. Chromatin immunoprecipitation-PCR confirmed that EjERF12 binds to the EjCAL promoter, and β-glucuronidase activity assays indicated that EjERF12 regulates EjCAL *** loquat trees with sorbitol promoted flower bud formation and was associated with increased expression of EjERF12 and EjCAL. Furthermore, we identified EjUF3GaT1 as a target gene of EjCAL and its expression was activated by EjCAL. Function characterization via overexpression and RNAi reveals that EjUF3GaT1 is a biosynthetic gene of flavonoid hyperoside. The concentration of the flavonoid hyperoside mirrored that of sorbitol during FBD and exogenous hyperoside treatment also promoted loquat bud formation. We identified a mechanism whereby EjCAL might regulate hyperoside biosynthesis and confirmed the involvement of EjCAL in flower bud formation in planta. Together,these results provide insight into bud formation in loquat and may be used in efforts to increase yield.

关键词： EjCAL flower bud differentiation hyperoside loquat sorbitol

Detection of skin defects on loquat using hyperspectral imaging combining both band radio and improved three-phase level set segmentation method

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Food Quality and Safety 2023年第1期7卷 100-111页

作者： Zhaoyang Han Bin Li Qiu Wang Zhaoxiang Sun Yande Liu School of Mechatronics&Vehicle Engineering East China Jiaotong UniversityNational and Local Joint Engineering Research Center of Fruit Intelligent Photoelectric Detection Technology and EquipmentNanchangChina

Background and objectives Skin defects are one of the primary problems that occur in post-harvest grading and processing of *** defects lead to the loquat being easily destroyed during transportation and storage,which causes the risk of other loquats being infected,affecting the selling *** and Methods In this paper,a method combining band radio image with an improved three-phase level set segmentation algorithm(ITPLSSM)is proposed to achieve high accuracy,rapid,and non-destructive detection of skin defects of *** component analysis(PCA)was used to find the characteristic wavelength and PC images to distinguish four types of skin *** best band ratio image based on characteristic wavelength was *** The band ratio image(Q782/944)based on PC2 image is the best segmented *** on pseudo-color image enhancement,morphological processing,and local clustering criteria,the band ratio image(Q782/944)has better contrast between defective and normal areas in ***,the ITPLSSM was used to segment the processing band ratio image(Q782/944),with an accuracy of 95.28%.Conclusions The proposed ITPLSSM method is effective in distinguishing four types of skin ***,it also effectively segments images with intensity inhomogeneities.

关键词： loquat skin defects hyperspectral imaging multispectral images band ratio improved three-phase level set segmentation.

Selection and Validation of Reference Genes for Normalization of RT-qPCR Analysis in Developing or Abiotic-Stressed Tissues of loquat (Eriobotrya japonica)

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Phyton-International Journal of Experimental Botany 2023年第4期92卷 1185-1201页

作者： Shoukai Lin Shichang Xu Liyan Huang Fuxiang Qiu Yihong Zheng Qionghao Liu Shiwei Ma Bisha Wu Jincheng Wu Fujian Provincial Key Laboratory of Ecology-Toxicological Effects&Control for Emerging Contaminants College of Environmental and Biological EngineeringPutian UniversityPutian351100China Key Laboratory of Ecological Environment and Information Atlas(Putian University) Fujian Provincial UniversityPutian351100China

loquat(Eriobotrya japonica Lindl.)is a subtropical evergreen fruit tree that produces fruits with abundant nutrients and medicinal *** suitable reference genes for a set of loquat samples before qRT-PCR experiments is essential for the accurate quantification of gene *** this study,eight candidate reference genes were selected from our previously published RNA-seq data,and primers for each candidate reference gene were designed and *** Cq values of the candidate reference genes were calculated by RT-qPCR in 31 different loquat samples,including 12 subgroups of developing or abiotic-stressed *** combinations of stable reference genes were screened according to a comprehensive rank,which was synthesized from the results of four algorithms,including the geNorm,NormFinder,BestKeeper andΔCt *** screened reference genes were verified by normalizing EjLGA1 in each *** obtained suitable combinations of reference genes for accurate normalization were GAPDH,EF1αand ACT for floral development;GAPDH,UBCE and ACT for fruit setting;EF1α,GAPDH and eIF2B for fruit ripening;ACT,EF1αand UBCE for leaves under heat stress;eIF2B,UBCE and EF1αfor leaves under freezing stress;EF1α,TUA and UBCE for leaves under salt stress;ACT,EF1αand eIF2B for immature pulp under freezing stress;ACT,UBCE and eIF2B for immature seeds under freezing stress;EF1α,eIF2B and UBCE for both immature pulp and seeds under freezing stress;UBCE,TUB and TUA for red-fleshed fruits under cold-storage stress;eIF2B,RPS3 and TUB for white-fleshed fruits under coldstorage stress;and eIF2B,UBCE and RPS3 for both red-and white-fleshed fruits under cold-storage *** study obtained different combinations of stable reference genes for accurate normalization in twelve subgroups of developing or abiotic-stressed tissues in *** our knowledge,this is the first report to obtain stable reference genes for normalizing gene expression of abiotic-stressed t

关键词： loquat Eriobotrya japonica reference genes qRT-PCR gene expression normalization developing tissues abiotic-stressed tissues

Genome-Wide Identification of the GST Gene Family in loquat (Eriobotrya japonica Lindl.) and Their Expression under Cold Stress with ALA Pretreatment

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Phyton-International Journal of Experimental Botany 2024年第11期93卷 2715-2735页

作者： Guanpeng Huang Ti Wu Yinjie Zheng Qiyun Gu Qiaobin Chen Shoukai Lin Jincheng Wu College of Life Sciences Fujian Agriculture and Forestry UniversityFuzhou350002China College of Environmental and Biological Engineering Putian UniversityPutian351100China College of JunCao Science and Ecology(College of Carbon Neutrality) Fujian Agriculture and Forestry UniversityFuzhou350002China

loquat(Eriobotrya japonica Lindl.),a rare fruit native to China,has a long history of cultivation in *** temperature is the key factor restricting loquat growth and severely affects *** temperature induces the regeneration and metabolism of reduced glutathione(GSH)to alleviate stress damage via the participation of glu-tathione S-transferases(GSTs)in *** this study,16 GSTs were identiﬁed from the loquat genome according to their protein sequence similarity with Arabidopsis *** the basis of domain characteristics and phyloge-netic analysis of AtGSTs,these EjGSTs can be divided into 4 subclasses:Phi,Theta,Tau and *** basic prop-erties,subcellular localization,structures,motifs,chromosomal distribution and collinearity of the EjGST proteins or genes were further *** and segmental gene duplications play pivotal roles in EjGST ***-elements that respond to various hormones and stresses,especially those associated with low-temperature responsiveness,were predicted to be present in the promoters of ***,analysis of gene expression proﬁles revealed that 9 of 16 EjGSTs may be involved in the low-temperature responsiveness of loquat *** agriculture,5-aminolevulinic acid(ALA),a potential multifunctional plant growth regulator,can improve the stress response of *** the 9 low-temperature-responsive EjGSTs,the expression of EjGSTU1 and EjGSTF1 signiﬁcantly differed under cold stress in response to exogenous 5-aminolevulinic acid(ALA)*** remarkable increase in GST activity and GSH/GSSG ratio reﬂected the increase in the cold response ability of loquat plants caused by exogenous ALA,thereby alleviating H2O2 accumulation and membrane lipid ***,this study provides an initial exploration of the cold tolerance function of GSTs in loquat,offering a theoretical foundation for the development of cold-resistant loquat cultivars and new antifreeze agents.

关键词： loquat GST gene family identiﬁcation gene expression cold stress ALA

Polyphenol Oxidase Inactivation by Microwave Oven and Its Effect on Phenolic Profile of loquat (<i>Eriobotrya japonica</i>) Fruit

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Food and Nutrition Sciences 2013年第9期4卷 87-94页

作者： Yanet Chávez-Reyes Lidia Dorantes-Alvarez Daniel Arrieta-Baez Obed Osorio-Esquivel Alicia Ortiz-Moreno Departamento de Ingeniería Bioquímica Escuela Nacional de Ciencias Biológicas Instituto Politécnico Nacional México D.F. México Espectrometría de Masas y RMN Centro de Nanociencias y Micro y NanoTecnología México D.F. México Instituto de Salud Pública Universidad de Chalcatongo Chalcatongo de Hidalgo Oaxaca México.

The objective of this research was investigated the effect of polyphenol oxidase microwave treatment on phenolic composition, antioxidant activity and microstructure of loquat fruit. Phenolic profile of methanolic extracts prepared from fresh, and microwave-treated samples were analyzed. Antioxidant activity was also evaluated by 2,2’-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS?+) and 1,1-diphenyl-2-picrylhydrazyl (DPPH+) methods. In addition, polyphenol oxidase inactivation was carried out using a response surface methodology to establish the optimal conditions of treatment. The phenolic content of fresh mesocarp was 311 ± 0.60 mg gallic acid equivalents (GAE)/100g dry weight (DW) and that of microwave-treated mesocarp was 1230 ± 0.36 mg GAE/100g DW. Total phenolic content of water/ methanol extract significantly increases after microwave treatment rather than methanolic extract of fresh loquat. Five glycoside phenolics were identified by HPLC-DAD-MS as 3-caffeoylquinic acid, 3-p-coumaroylquinic acid, 5-caffeoylquinic acid and quercetin-3-O-sambubioside. Methanolic extract of microwave-treated mesocarp showed higher antioxidant activity than that of fresh mesocarp. Thus, polyphenol oxidase inactivation by microwave energy preserved the integrity of phenolic compounds as well as antioxidant activity in mesocarp extracts prepared from loquat fruit. It was also noted that phenolics were more abundant in the microwaved samples than in the fresh samples.

关键词： loquat Phenolic Compounds Polyphenol Oxidase Microwave Eriobotrya japonica

"One Brand for One Village"Planning of Qingzhong loquat Sightseeing Park in Jinting Township of Suzhou City,China

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Journal of Landscape Research 2011年第8期3卷 32-36,41页

作者：余俊潘文明朱笑曹纯苏州农业职业技术学院江苏苏州215008

The planning and design of Qingzhong loquat Sightseeing Park in Jinting Township, Wuzhong District, Suzhou City was taken for an example, industrial development, advantages and disadvantages of the study area were analyzed. Design concepts and techniques of"One Brand for One Village"for Qingzhong loquat Sightseeing Park were introduced from the perspectives of project orientation, planning concepts, functional division, scenic spot design, infrastructure planning and so on. Six ancient relics and three grotesque trees were introduced to expound the planning scheme of typical landscapes. Finally, the architectural control planning from the perspectives of height and style was stressed. The planning scheme broke common restraints of other similar parks, such as poor infrastructure and disordered garden constructions, proposed the planning concept of"building a modern eco-agricultural sightseeing fruit park mainly for production but also integrating functions of tourism, ecological picking, recreation and entertainment", providing a basis for building "One Brand for One Village" and realizing the high-efficiency scale-development of agriculture.

关键词： Suzhou One Brand for One Village loquat Sightseeing Fruit Park

Hormone responses of loquat plant to Trichoderma P3.9 and pathogen of loquat Root Rot

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Hormone responses of loquat plant to Trichoderma P3.9 and pa...

Characterization of Carotenoid Accumulation and Carotenogenic Gene Expression During Fruit Development in Yellow and White loquat Fruit

中国植物病理学会2021年学术年会

作者： LU Haiju ZHU Haiyan XIONG Xinyan FENG Yu ZHAO Mengjuan Department of Life Science and Technology Honghe College

In order to find out the difference of hormone responses of loquat plant to the beneficial fungi and the pathogenic fungi,and to determine the signal transduction pathway of loquat plant induced by the endophytic Trichoderma P3.9 to resist the pathogen of root *** P3.9 and the pathogen of loquat of root rot P3.1,P3.5 and P3.6 strains were used as research *** the 1 year loquat seedlings of root innoculated by single T.P3.9 as the treatment group,by mixture with the T.P3.9 and the respective pathogen of loquat root rot P3.1,P3.5 and P3.6 strains as the positive group,and without inoculation as the negative control *** dosages are respective 20 g T.P3.9 biological agent and3 mycelia pieces diameter 5 mm each loquat *** changes of jasmonic acid,free salicylic acid and combinative salicylic acid in loquat roots,stems and leaves were tested by high performance liquid *** results showed that *** P3.9 interacted with loquat plant,which increased the content of JA,free SA and bound SA in loquat roots and *** content of JA in leaves was not affected,but that of free SA was decreased,and the content of bound SA was *** indicates that *** P3.9 activates both JA and SA signaling molecules to initiate the defense system of loquat *** P3.9 interacted with the pathogen of loquat root rot,which increased the contents of JA,free SA and bound SA in stems,and the content of free SA in *** indicates that when loquat plant was attacked by the pathogen of loquat root rot,*** P3.9 can maintain high content of JA and SA in loquat stem,and activate the defense system of it,which had synergistic effect on the increase of SA content in loquat root,so as to resist the attack of the pathogen of loquat root *** T.P3.9 respectively interacted with the pathogens P3.1,P3.5 and P3.6,it was not consistent that the change of JA content in *** JA content of the former was decreased,but the latter was

关键词： Trichoderma loquat root rot jasmonic acid salicylic acid high performance liquid chromatography

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Horticultural Plant Journal 2016年第1期2卷 9-15页

作者： ZHANG Ling ZHANG Zhike ZHENG Tingting WEI Weilin ZHU Yunmei GAO Yongshun YANG Xianghui LIN Shunquan College of Horticulture South China Agricultural University Shangrao City Forest Resources Monitoring Center

Accumulation of carotenoids in peel and pulp of the yellow-fleshe loquat ‘Zaozhong 6'(ZZ6) and the white-fleshe loquat ‘Baiyu'(BY) were tracked during different fruit development stages, and the expression of 15 carotenogenic genes were analyzed. During loquat fruit ripening the fresh weight content of β-carotene in peel and pulp of ZZ6 increased gradually and peaked at the fully ripe stage, reaching 68.53 μg·g^(-1)FW in the peel and 11.92 μg·g^(-1)FW in the pulp. In BY, the content of β-carotene in the peel increased and peaked at the fully ripe stage, reaching38.89 μg·g^(-1)FW, while it decreased in the pulp from the original 0.47 μg·g~(^(-1))FW and reduced to 0.29 μg·g~(^(-1))FW. The content of β-cryptoxanthin in the peel and pulp of ZZ6 and BY both increased steadily, and peaked at the fully ripe stage; however, the content of lutein decreased in the peel of ZZ6 and increased in the pulp, but in BY, it dropped and then rose in the peel. There was no significan change of β-cryptoxanthin in the pulp of BY. After the breaker stage, the m RNA levels of phytoene synthase(PSY) and chromoplast-specifi lycopene β-cyclase(CYCB) were higher in the peel, while CYCB and β-carotene hydroxylase(BCH) m RNAs were higher in the fles of ZZ6, compared with BY. The results showed that the expression level of PSY, CYCB, and BCH appeared to cooperatively regulate the accumulation of carotenoids.

关键词： Eriobotrya japonica loquat carotenoids gene expression