Characterization of Carotenoid Accumulation and Carotenogenic Gene Expression During Fruit Development in Yellow and White Loquat Fruit

作     者：ZHANG Ling ZHANG Zhike ZHENG Tingting WEI Weilin ZHU Yunmei GAO Yongshun YANG Xianghui LIN Shunquan 

作者机构：College of Horticulture South China Agricultural University Shangrao City Forest Resources Monitoring Center 

出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)

年 卷 期：2016年第2卷第1期

页      面：9-15页

学科分类：09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：partially supported by Guangzhou Science and Technology Program key projects(201504010028) 

主　　题：Eriobotrya japonica loquat carotenoids gene expression 

摘      要：Accumulation of carotenoids in peel and pulp of the yellow-fleshe loquat ‘Zaozhong 6 (ZZ6) and the white-fleshe loquat ‘Baiyu (BY) were tracked during different fruit development stages, and the expression of 15 carotenogenic genes were analyzed. During loquat fruit ripening the fresh weight content of β-carotene in peel and pulp of ZZ6 increased gradually and peaked at the fully ripe stage, reaching 68.53 μg·g^(-1)FW in the peel and 11.92 μg·g^(-1)FW in the pulp. In BY, the content of β-carotene in the peel increased and peaked at the fully ripe stage, reaching38.89 μg·g^(-1)FW, while it decreased in the pulp from the original 0.47 μg·g~(^(-1))FW and reduced to 0.29 μg·g~(^(-1))FW. The content of β-cryptoxanthin in the peel and pulp of ZZ6 and BY both increased steadily, and peaked at the fully ripe stage; however, the content of lutein decreased in the peel of ZZ6 and increased in the pulp, but in BY, it dropped and then rose in the peel. There was no significan change of β-cryptoxanthin in the pulp of BY. After the breaker stage, the m RNA levels of phytoene synthase(PSY) and chromoplast-specifi lycopene β-cyclase(CYCB) were higher in the peel, while CYCB and β-carotene hydroxylase(BCH) m RNAs were higher in the fles of ZZ6, compared with BY. The results showed that the expression level of PSY, CYCB, and BCH appeared to cooperatively regulate the accumulation of carotenoids.