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Lectin Conjugated Gold Nanoparticle-based Colorimetric Assay for Studying the Interactions of Antibiotic with living cell

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Chemical Research in Chinese Universities 2011年第2期27卷 193-197页

作者： WANG Jin-e WANG Cheng-ke LIU Dian-jun WANG Zhen-xin State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 P. R. China Graduate School of Chinese Academy of Sciences Beijing 100049 P. R. China

The interactions of antibiotic with living cells were studied by lectin conjugated gold nanoparticles（GNPs） based colorimetric assay. Because of the high affinity of lectin for saccharides, the lectin conjugated GNPs are able to employ as indicators for monitoring the antibiotic induced changes of glycosyl complexes. The interactions of a well known antibiotic, tunicamycin, with two different cell lines, HeLa and SHG-44, were selected to establish this assay. In the presence of tunicamycin, the dose- and time-dependence on the decreasing of binding affinity of lectin conjugated GNPs with living cells were demonstrated by conventional microscopic and UV-Vis spectroscopic studies. The experimental result demonstrates that our approach can be used to identify antibiotic induced expression difference of glycosyl complexes on different cellular surfaces and determine drug activity quantitatively. For further confirming the capability of the GNP-based assay, the system was also studied by confocal laser scanning microscopy（CLSM） and classic flow cytometry（FCM） assay, and satisfactory results were obtained.

关键词： Lectin-conjugated gold nanoparticle Tunicamycin Colorimetric assay living cell

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Iron-Imprinted Single-Atomic Site Catalyst-Based Nanoprobe for Detection of Hydrogen Peroxide in living cells

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Nano-Micro Letters 2021年第9期13卷 364-376页

作者： Zhaoyuan Lyu Shichao Ding Maoyu Wang Xiaoqing Pan Zhenxing Feng Hangyu Tian Chengzhou Zhu Dan Du Yuehe Lin School of Mechanical and Materials Engineering Washington State UniversityPullmanWA 99164USA School of Chemical Biologicaland Environmental EngineeringOregon State UniversityCorvallisOR 97331USA Irvine Materials Research Institute(IMRI) University of CaliforniaIrvineCA 92697USA

Fe-based single-atomic site catalysts(SASCs),with the natural metalloproteases-like active site structure,have attracted widespread attention in biocatalysis and ***,controlling the isolated single-atom Fe-N-C active site structure is crucial to improve the SASCs’*** this work,we use a facile ion-imprinting method(IIM)to synthesize isolated Fe-N-C single-atomic site catalysts(IIM-Fe-SASC).With this method,the ion-imprinting process can precisely control ion at the atomic level and form numerous well-defined single-atomic Fe-N-C *** IIM-Fe-SASC shows better peroxidase-like activities than that of non-imprinted *** to its excellent properties,IIM-Fe-SASC is an ideal nanoprobe used in the colorimetric biosensing of hydrogen peroxide(H_(2)O_(2)).Using IIM-Fe-SASC as the nanoprobe,in situ detection of H_(2)O_(2)generated from MDA-MB-231 cells has been successfully demonstrated with satisfactory sensitivity and *** work opens a novel and easy route in designing advanced SASC and provides a sensitive tool for intracellular H_(2)O_(2)detection.

关键词： Single-atomic site catalysts Nanoprobe Peroxidase-like activities Biosensing living cell

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Real-time detection of α1A-AR movement stimulated by phenylephrine in single living cells

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Acta Pharmacologica Sinica 2007年第6期28卷 796-802页

作者： Ning XU~(2,5),Zhang-yi LIANG~(3,5),Ming XU~2,Ying-hua GUAN~3,Qi-hua HE~4,Qi-de HAN~2,Xin-sheng ZHAO~(3,6),You-yi ZHANG~(2,6)~2 Institute of Vascular Medicine,Peking University Third Hospital,and Key Laboratory of Molecular Cardiovascular Sciences,Ministry ofEducation,Beijing 100083,China ~3 Beijing National Laboratory for Molecular Sciences,State Key Laboratory for Structural Chemistry ofUnstable and Stable Species,and Department of Chemical Biology,College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,China ~4 Center of Medical Analysis,Peking University,Health Science Center,Beijing 100083,China Institute of Vascular Medicine Peking University Third Hospital and Key Laboratory of Molecular Cardiovascular Sciences Ministry of Education Beijing China Beijing National Laboratory for Molecular Sciences State Key Laboratory for Structural Chemistry of Unstable and Stable Species Department of Chemical Biology College of Chemistry and Molecular Engineering Peking University Beijing China Center of Medical Analysis Peking University Health Science Center Beijing China

Aim:To investigate the movement of α1A-adrenergic receptors(α1A-AR) stimu-lated by agonist,phenylephrine （PE）,and the dynamics of receptor movement inreal time in single living cells with millisecond ***:We labeledα1A-AR using the monoclonal,anti-FLAG （a kind of tag） antibody and Cy3-conju-gated goat anti-mouse IgG and recorded the trajectory of their transport processin living HEK293A cells stimulated by agonist,PE,and then analyzed their dy-namic ***:The specific detection of α1A-AR on the surface ofliving HEK293A-α1Acells was achieved.α1A-AR internalize under the stimulationof *** the cells were stimulated with PE for 20 min,apparent colocalizationwas found between α1A-AR and *** 40 min stimulation of PE,trajecto-ries of approximate linear motion in HEK293A-α1Acells were recorded,and theirvelocity was ***:The specific labeling method on the livingcell surface provides a convenient means of real-time detection of the behavior ofsurface *** this method we were able to specifically detect α1A-AR andrecord the behavior of individual particles of receptors with 50 ms exposure time inreal time in single living cells.

关键词： adrenergic receptor trajectory living cell

Experimental observation of living cells on the surface of intraocular lenses

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吉林大学学报(医学版) 2000年第2期26卷 142-143页

作者： Yu Hongquan,Zhang Yan,Yu Jun (Department of Ophthalmology,The First Teaching Hospital of Norman Bethune University of Medical Sciences,Changchun 130021)〔白求恩医科大学吉林长春 130021

目的 :对比观察后房型人工晶状体植入术后晶状体表面细胞染色前后的数量、形态。方法 :2 0只兔行后房型人工晶状体植入术。分别于术后 1 ,3,7,1 4和 2 8d分组取出人工晶状体放入1 640细胞培养液于倒置显微镜下观察。结果 :术后 1～ 2 8... 详细信息

目的 :对比观察后房型人工晶状体植入术后晶状体表面细胞染色前后的数量、形态。方法 :2 0只兔行后房型人工晶状体植入术。分别于术后 1 ,3,7,1 4和 2 8d分组取出人工晶状体放入1 640细胞培养液于倒置显微镜下观察。结果 :术后 1～ 2 8d人工晶状体表面有细胞粘附 ,染色后细胞形态失去立体外观 ,体积小 ,细胞数量较染色前明显减少。结论 :人工晶状体表面细胞在染色过程中有部分脱落。脱落的细胞数量可以计数比较 ,但细胞类型无法确定。因此需寻找更精确可靠的仪器及方法 ,以免造成实验误差。

关键词： ? intraocular lens living cell

A Turn-On Fluorescent Probe for Highly Selective and Sensitive Detection of Palladium

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Chinese Journal of Chemistry 2016年第7期34卷 715-719页

作者： Junliang Zhou Jian Zhang Hang Ren Xiaochun Dong Xing Zheng Weili Zhao Institute of Pharmacy and Pharmacology University of South China Hengyang Hunan 421001 China School of Pharmacy Fudan University Shanghai 201203 China Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study Hengyang Hunan 421001 China

A novel turn-on fluorescent probe for the detection of palladium has been designed. The probe can selectively and sensitively detect palladium in solution, and the limit of detection was calculated to be 11.4 nmol·L-... 详细信息

关键词： fluorescent probe palladium living cell

Furin controlled intracellular assembly of nanoparticles for enhanced uptake of radionuclide in living cells

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Furin controlled intracellular assembly of nanoparticles for...