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Cathepsin D knockdown regulates biological behaviors of granulosa cells and affects litter size traits in goats

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2021年第11期22卷 893-905页

作者： Zhinan ZHOU Xiang CHEN Min ZHU Weiwei WANG Zheng AO Jiafu ZHAO Wen TANG Lei HONG Key Laboratory of Plateau Mountain Animal Genetics Breeding and Reproduction of Ministry of EducationKey Laboratory of Animal GeneticsBreeding and Reproduction of Guizhou ProvinceCollege of Animal ScienceGuizhou UniversityGuiyang 550025China

Cathepsin D(CTSD),the major lysosomal aspartic protease that is widely expressed in different tissues,potentially regulates the biological behaviors of various *** granulosa cells are responsive to the increase of ovulation number,hence indirectly influencing litter ***,the mechanism underlying the effect of CTSD on the behaviors of goat granulosa cells has not been fully *** study used immunohistochemistry to analyze CTSD localization in goat ovarian ***,western blotting was applied to examine the differential expression of CTSD in the ovarian tissues of monotocous and polytocous ***,the effects of CTSD knockdown on cell proliferation,apoptosis,cell cycle,and the expression of candidate genes of the prolific traits,including bone morphogenetic protein receptor IB(BMPR-IB),follicle-stimulating hormone(FSHR),and inhibinα(INHA),were determined in granulosa *** showed that CTSD was expressed in corpus luteum,follicle,and granulosa ***,CTSD expression in the monotocous group was significantly higher than that in the polytocous *** addition,CTSD knockdown could improve granulosa cell proliferation,inhibit cell apoptosis,and significantly elevate the expression of proliferating cell nuclear antigen(PCNA)and B cell lymphoma 2(Bcl-2),but it lowered the expression of Bcl-2-associated X(Bax)and ***,CTSD knockdown significantly reduced the ratios of cells in the G0/G1 and G2/M phases but substantially increased the ratio of cells in the S *** expression levels of cyclin D2 and cyclin E were elevated followed by the obvious decline of cyclin A1 ***,the expression levels of BMPR-IB,FSHR,and INHA clearly increased as a result of CTSD ***,our findings demonstrate that CTSD is an important factor affecting the litter size trait in goats by regulating the granulosa cell proliferation,apoptosis,cell cycle,and the expression of candidate genes of th

关键词： Cathepsin D(CTSD) litter size trait Granulosa cells Cell apoptosis Cell cycle Cell proliferation

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