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Chemical compounds as well as antioxidant and litholytic activities of Arbutus unedo L. leaves against calcium oxalate stones

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Journal of Integrative Medicine 2019年第6期17卷 430-437页

作者： Rabie Kachkoul Tarik Squalli Housseini Mohamed Mohim Radouane El Habbani Youssef Miyah Anissa Lahrichi Laboratory of Biochemistry Faculty of Medicine and PharmacyUniversity Sidi Mohammed Ben AbdellahBP 1893FezMorocco Faculty of Science and Technology University Sidi Mohammed Ben AbdellahBP 2202FezMorocco Laboratory of Molecular Bases in Human Pathology and Therapeutic Tools Faculty of Medicine and PharmacyUniversity Sidi Mohammed Ben AbdellahBP 1893FezMorocco Department of Nephrology University Hospital HassanⅡBP 1835AtlasFezMorocco

Objective: The present study aimed to quantify and identify the bioactive compounds of the Arbutus unedo L. leaves in order to evaluate both their antioxidant properties and litholytic activities against calcium oxalate ***: This survey was carried out using hydroalcoholic extract(***) and infusion(***) of A. unedo leaves. The quantification of phenolic compounds, flavonoids, flavonols and anthocyanins was done by spectrophotometric methods and identification of chemical components was performed by ultraperformance liquid chromatography with photodiode array and electrospray ionization tandem mass spectrometry. Antioxidant activity was measured using the 1,1-diphenyl-2-picrylhydrazyl(DPPH)method and by the ferric reducing/antioxidant power(FRAP) assay. litholytic activity of *** and *** was studied using a special model that resembles circuitry of the urinary ***: *** showed greater antioxidant efficacy than ***(P litholytic activity compared to ***(P < 0.05), with dissolution values of 31.03% ± 0.63% versus 14.55% ± 0.65%, ***: Overall, the results suggest that the A. unedo is rich in bioactive compounds, and possesses antioxidant and litholitic abilities that are worthy of further study.

关键词： Antioxidant activity Arbutus unedo L litholytic activity Phenolic compounds Liquid chromatography Photodiode array Electrospray ionization tandem Mass spectrometry

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