Chemical compounds as well as antioxidant and litholytic activities of Arbutus unedo L. leaves against calcium oxalate stones

作     者：Rabie Kachkoul Tarik Squalli Housseini Mohamed Mohim Radouane El Habbani Youssef Miyah Anissa Lahrichi 

作者机构：Laboratory of BiochemistryFaculty of Medicine and PharmacyUniversity Sidi Mohammed Ben AbdellahBP 1893FezMorocco Faculty of Science and TechnologyUniversity Sidi Mohammed Ben AbdellahBP 2202FezMorocco Laboratory of Molecular Bases in Human Pathology and Therapeutic ToolsFaculty of Medicine and PharmacyUniversity Sidi Mohammed Ben AbdellahBP 1893FezMorocco Department of NephrologyUniversity Hospital HassanⅡBP 1835AtlasFezMorocco 

出 版 物：《Journal of Integrative Medicine》 (结合医学学报（英文版）)

年 卷 期：2019年第17卷第6期

页      面：430-437页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学] 

主　　题：Antioxidant activity Arbutus unedo L Litholytic activity Phenolic compounds Liquid chromatography Photodiode array Electrospray ionization tandem Mass spectrometry 

摘      要：Objective: The present study aimed to quantify and identify the bioactive compounds of the Arbutus unedo L. leaves in order to evaluate both their antioxidant properties and litholytic activities against calcium oxalate ***: This survey was carried out using hydroalcoholic extract(***) and infusion(***) of A. unedo leaves. The quantification of phenolic compounds, flavonoids, flavonols and anthocyanins was done by spectrophotometric methods and identification of chemical components was performed by ultraperformance liquid chromatography with photodiode array and electrospray ionization tandem mass spectrometry. Antioxidant activity was measured using the 1,1-diphenyl-2-picrylhydrazyl(DPPH)method and by the ferric reducing/antioxidant power(FRAP) assay. Litholytic activity of *** and *** was studied using a special model that resembles circuitry of the urinary ***: *** showed greater antioxidant efficacy than ***(P 0.05). Its higher efficiency was shown via the values of median inhibitory concentration, which was close to(76.14 ± 0.91) mg/mL for *** versus(202.64 ± 5.77) lg/mL for *** using the DPPH method, and(53.77 ± 0.81) lg/mL for *** versus(236.86 ± 31.90) lg/mL for ***, using FRAP method. *** exhibited significantly higher litholytic activity compared to ***(P 0.05), with dissolution values of 31.03% ± 0.63% versus 14.55% ± 0.65%, ***: Overall, the results suggest that the A. unedo is rich in bioactive compounds, and possesses antioxidant and litholitic abilities that are worthy of further study.