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Temperature-regulated typeⅡgrass carp reovirus establishes latent infection in Ctenopharyngodon idella brain

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Virologica Sinica 2023年第3期38卷 440-447页

作者： Rui Jiang Jie Zhang Zhiwei Liao Wentao Zhu Hang Su Yongan Zhang Jianguo Su College of Fisheries Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan430070China Laboratory for Marine Biology and Biotechnology Pilot National Laboratory for Marine Science and TechnologyQingdao266237China

Grass carp reovirus(GCRV)causes extensive infection and death in grass carp and black carp fingerlings,with a highly seasonal prevalence.Previous studies suggested that GCRV can become latent after primary infection.In this study,we investigated type II GCRV(GCRV-II)latency in asymptomatic grass carp with GCRV infection or exposure history.We found that during latent infection,GCRV-II was detectable only in the brain of grass carp,unlike the multi-tissue distribution observed in natural infection.GCRV-II only caused damage to the brain during latent infection,while in natural infection,brain,heart,and eye tissues had relatively higher viral loads.We also discovered viral inclusion bodies in infected fish brains.Additionally,GCRV-II distribution in grass carp was notably affected by ambient temperature,with the virus targeting the brain only during low temperatures and multi-tissue distribution during high temperatures.This study provides insights into the mechanisms of GCRV-II latent infection and reactivation and contributes to the prevention and control of GCRV pandemics.

关键词： Grass carp Grass carp reovirus(GCRV) latent infection Brain

High frequency of latent Chlamydia trachomatis infection in patients with rhegmatogenous retinal detachment

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International Journal of Ophthalmology(English edition) 2016年第6期9卷 863-868页

作者： Ernest V.Bolko Alexei L.Pozniak Dmitrii S.Maltsev Alexei A.Suetov Irina V.Nuralova St.Petersburg Branch of the Academician S.Fyodorov IRTC "Eye Microsurgery"St.Petersburg 192283Russia Department of Ophthalmology Military Medical AcademySt.Petersburg 194044Russia

AIM： To determine the frequency of detection of ocular and extraocular Chlamydia trachomatis （CT） infection in non -high myopes with rhegmatogenous retinal detachment （RRD）. METHODS： This was a single-center, nonrandomized, prospective, case-control study. One hundred and four patients were divided into a study group with RRD （n= 63） and a control group with traumatic retinal detachment （n=41）. Samples of subretinal fluid （SFR）, conjunctival, urethral/cervical swabs, and blood were collected. The frequency of detection of CT infection in SRF samples was determined by polymerase chain reaction （PCR）, direct fluorescence assay （DFA） and cell culture, whereas that in conjunctival swabs was determined by PCR and DFA, and those in urethral/cervical swabs and blood were determined by DFA. Yates Chi-square test （with Bonferroni correction） and two-tailed Student＇s t-test were used for statistical analysis. RESULTS： SRF CT infection was detected more frequently in the study group （50.8%-71.4%） than in the control group （9.8%-12.2%） by all the methods used （P〈 0.01）. The frequency of detection of conjunctival CT infection by DFA was higher in the RRD patients compared with the controls （81.0% vs 24.4%, P=0.004）. The PCR detected conjunctival CT infection more often in the study group than in the controls （46.0% vs89.8%, P= 0.007）. The DFA detected CT in blood specimens almost as frequently as in urogenital specimens, for the RRD patients （61.2% vs 63.5%） and the controls （7.3% vs 9.8%）. CONCLUSION： CT infection is detected with high frequency in non-high myopes with RRD.

关键词： rhegmatogenous retinal detachment Chlamydia trachomatis myopia latent infection

Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples

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Journal of Coastal Life Medicine 2014年第2期2卷 125-131页

作者： Jorge G Fernández Martín A Fernández-Baldo Claudio Muñoz Eloy Salinas Julio Raba María I Sanz Area of Chemistry and Biotechnology Technology Department of ChemistryFaculty of ChemistryBiochemistry and PharmacyNational University of San LuisChacabuco 917D5700BWS San LuisArgentina Inquisal-Conicet Department of ChemistryFaculty of ChemistryBiochemistry and PharmacyNational University of San LuisChacabuco 917D5700BWS San LuisArgentina Laboratory of Plant Virology EEA Mendoza INTASan Martín 3853D5507BWSMendozaArgentina

Objective:To detect Botrytis cinerea(B.cinerea)latent infections on apples before storage,which is essential for effective control strategies in the fruit postharvest industry.Methods:In the present study,a polymerase chain reaction detection method,based on primers designed on B.cinerea transposable elements(boty and flipper)and intergenic spacer region as internal control,were utilized to reveal the presence of symptomless infections on apple fruits.This molecular method proved to be highly specific and sensitive in detecting latent infections.It revealed the presence of the pathogen in 83%of the samples from infected apples with 104 conidia/mL,whereas those infected with 106 conidia/mL detected 94%as compared to the traditional method that revealed the pathogen in 40%and 66%of the samples inoculated with 10^(4)and 10^(6)conidia/mL respectively.Furthermore,the method characterized B.cinerea as subpopulation transposa-type by the presence of the transposable elements boty and flipper Results:The results obtained from DNA quantification method were compared with enzyme-linked immunosorbent assay and these studies showed good correlation.Therefore our method has important advantages compared with others detection methods for B.cinerea,because the proposed methodology allowed distinguishes between its two subpopulations(vacuma and transposa)and this would allow establish possible appropriate control strategies.Conclusions:Finally,the method can be an interesting alternative for its possible application in the phytosanitary programs of the fruit industry worldwide.

关键词： Botrytis cinerea latent infection Transposable elements Boty and flipper Symptomless apples

Reservoir of human immunodeficiency virus in the brain:New insights into the role of T cells

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BIOCELL 2023年第12期47卷 2591-2595页

作者： YINGDONG ZHANG MING CHU HONGZHOU LU Department of Neurosurgery The Second Affiliated HospitalSchool of MedicineSouthern University of Science and TechnologyShenzhen518055China School of Medicine The Second Affiliated HospitalShenzhen518000China Department of Infectious Diseases National Clinical Research Center for Infectious DiseasesShenzhen Third People’s HospitalShenzhen518000China

Human immunodeficiency virus(HIV)infection of the central nervous system(CNS)has attracted significant attention because it contributes to severe complications of acquired immunodeficiency syndrome(AIDS)and seriously impairs the life quality of infected patients.In this review,we briefly describe the latent infection of HIV in CNS and focus on the role of the important immune cells,such as T cells,in the formation and maintenance of the HIV reservoir in CNS.This review explores the mechanisms by which T cells enter CNS and establish latent infection of HIV in the CNS.In conclusion,we summarize the role of these cells in the interaction between HIV and CNS.With our better understanding of the underlying mechanisms,we propose future directions for the development of novel strategies to eliminate HIV reservoirs in the CNS based on cellular components.

关键词： Human immunodeficiency virus Central nervous system Reservoir latent infection T cells

Establishment and Application of a SYBR Green I Real-time Quantitative PCR Assay for the Detection of LAT Gene of Pseudorabies Virus in Bama Miniature Pigs

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Agricultural Biotechnology 2015年第3期4卷 62-66页

作者： Sufang LU Guangjun GUO Ling MO Feng WEI Bingmei DONG Wentong ZHANG Zhiqiang SHEN Shandong Binzhou Animal Science & Veterinary Medicine Academy/Shandong Research & Development Center of Veterinary Propolis Vaccines Shandong Lvdu Bio-Sciences & Technology Co. Ltd.

[ Objective] This study aimed to establish a rapid, sensitive and specific SYBR Green I real-time quantitative PCR assay for the detection of latent pseudorabies virus （PRV） infection. [ Method ] SYBR Green I real-time quantitative PCR conditions and system for early detection of latent pseudorabies virus in- fection were optimized and compared with conventional PCR to investigate the sensitivity and specificity. Subsequently, the established assay was applied to detect different clinical samples. [ Result] The sensitivity of SYBR Green I real-time quantitative PCR assay （52 copies/μl） was 1 000 times higher than that of conven- tional PCR （5.2×1^04 copies/μl） and the detection time was shortened by 1/2. The established assay could be used to detect PRV but could not be used to detect PCV2, PPV, CSFV or PRRSV. Various tissues were collected from Bama miniature pigs with latent PRV infection under sterile conditions for real-time PCR detection. Results showed that viral copy number in the brain, nasal swab, inguinal lymph node, liver, lung and spleen was above 20, while PRV was not detected in the kidney and heart tissues. [ Conclusion] The established SYBR Green I real-time quantitative PCR assay for PRV/.AT detection was specific, sensitive and rapid, which could be used for pathogen monitoring, epidemiological investigation and quantitative study of PRV.

关键词： SYBR Green I Pseudorabies virus gE deleted virus LAT gene latent infection

Morphological, Pathological, Biochemical and Molecular Characterization of <i>Ralstonia solanacearum</i>Isolates in Bangladesh

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American Journal of Molecular Biology 2021年第4期11卷 142-164页

作者： S. Razia M. S. M. Chowdhury F. M. Aminuzzaman N. Sultana M. Islam Department of Plant Pathology Faculty of Agriculture Sher-e-Bangla Agricultural University Dhaka Bangladesh Plant Pathology Division Bangladesh Agricultural Research Institute Joydebpur Gazipur Bangladesh

One thirty samples (fifty-five potato tubers, twenty-seven potato stems, three chili stems, twenty-eight soil samples, five weed samples, three banana leaves, and nine water samples) were examined and one hundred six (106) Bangladeshi isolates of Ralstonia solanacearum were isolated and identified. Isolation was made on selective media (Tetrazolium chloride media) and R. solanacearum was identified based on morphological, pathological and biochemical properties and polymerase chain reaction (PCR) by using the species-specific primers. Studies showed that 81.54% (106) samples were positive on tetrazolium chloride solid medium. Among them 90 isolates were virulent and rest of them were avirulent. Fifty isolates were selected for chemical characterization based on hypersensitivity test. R. solanacearum is gram negative, aerobic facultative bacteria on the basis of chemical characterization. Fifty tested isolates expressed as race 3 while in biovar test forty-eight showed as biovar III and the rest two showed as biovar I. In nine tested isolates from the three districts a species-specific band of 280 bp was amplified in PCR that confirmed the identity of R. solanacerum.

关键词： Brown Rot of Potato latent infection Ralstonia solanacearum Biovar Race

ULb'Region May Be Not Necessary For Establishment of HCMV latency model

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ULb'Region May Be Not Necessary For Establishment of HCMV la...