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Journal of Coastal Life Medicine 2016年第1期4卷 53-55页

作者： Ramar Ganasekar Jeyasankar Alagarmalai PG&Research Department of Zoology Arignar Anna Government Arts CollegeMusiri-621211Tamil NaduIndia PG&Research Department of Zoology Government Arts College(Autonomous)Coimbatore-641018Tamil NaduIndia

Objective:To determine the phytochemical and larvicidal activity of Tragia involucrata(T.involucrata)leaf extracts against fourth instar larvae of Ae.aegypti.Methods:Phytochemical analysis of hexane leaf extract was performed and larvicidal activity was determined against Ae.aegypti at concentrations of 50,100,150,200 and 250 mg/L.Larval mortality was assessed after 24 h.Results:The hexane extracts of T.involucrata was found to be higher mortality against the larvae of Ae.aegypti with a LC_(50) value of 153.51 mg/L.Conclusions:These results suggested that the leaf extracts of T.involucrata showed potential to be used as an ideal ecofriendly approach for the control of the Ae.aegypti.

关键词： Phytochemical larvicidal Tragia involucrata Leaf extract Aedes aegypti

Mosquito larvicidal properties of Ficus henghalensis L.(Family:Moraceae) against Culex tritaeniorhynchus Giles and Anopheles subpictus Grassi(Diptera:Culicidae)

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Asian Pacific Journal of Tropical Medicine 2011年第7期4卷 505-509页

作者： M Govindarajan R Sivakumar A Amsath S Niraimathi Division of Vector Biology and phytochemistry Department of ZoologyAnnamalai UniversityAnnamalainagar-608002India PG & Research Department of Zoology Khadir Mohideen CollegeAdirampattinam-614701TamilnaduIndia PG & Research Department of Biochemistry RVS College of Arts and ScienceKaraikal-609 609TamilnaduIndia

Objective:To determine the larvicidal efficacy of different solvent leaf extract of Ficus benghalensis（F.benghalensis） against Culex tritaeniorhynchus（Cx.tritaeniorhynchus） and Anopheles subpictus（An.subpictus）.Methods:Twenty five early third instar larvae of Cx. tritaeniorhynchus and An.subpictus were exposed to various concentrations and were assayed in the laboratory by using the protocol of WHO 2005.The larval mortality was observed after 24 h of treatment.Results:Among three solvent extracts tested the maximum efficacy was observed in the methanol extract.The LC50 and LC90 values of F.benghalensis against early third instar of Cx. tritaeniorhynchus and An.subpictus were 100.***.159.76 ppm and 56.***.85.84 ppm.respectively. No mortality was observed in controls.The chi-square values were significant at P<0.05 level. Conclusions:From the results it can be concluded the crude extract of F.benghalensis was an excellent potential for controlling Cx.tritaeniorhynchus and Art.subpictus mosquito larvae.

关键词： larvicidal activity Culex tritaeniorhynchus Anopheles subpictus Finis benghalensis

In VitroOvicidal and larvicidal Activities of Stem Bark of Terminalia glaucescens(Combretaceae) against Haemonchus contortus

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American Journal of Plant Sciences 2014年第19期5卷 2859-2868页

作者： Mbafor Fidelia Lem Khan Payne Vincent Wabo Pone Josue Yondo Jeannette Mbogning Tayo Gertrude Tchoumboue Joseph Laboratory of Applied Biology and Ecology Department of Animal Biology Faculty of Science University of Dschang Dschang Cameroon Laboratory of Animal Health Department of Animal Production Faculty of Agronomy and Agricultural Sciences University of Dschang Dschang Cameroon

Objectives: To evaluate the in vitro activity of aqueous and methanolic extracts of stem bark of Terminalia glaucescens on the eggs and larval stages of Haemonchus contortus of sheep. Methods: The eggs were exposed for 24 hours in five different concentrations (625, 1250, 2500, 3750 and 5000 μg/ml) of methanol, hot water (decoction) and cold water extracts at room temperature (25°C). Distilled water, 1.25% DMSO and Albendazole were used as negatives and positive control respectively in the bioassay. Results: A significant effect was obtained with all of the three extracts and differences were observed depending on the parasitic stage. Hot water extract (HWE), Methanol (MET) and Cold water extract (CWE) inhibited embryonic development by 98.1%, 96% and 86.5% respectively at 5000 μg/ml, meanwhile Albendazole had a 77.9% inhibition. For the mean inhibition rate of egg hatching, there was a general increase in the inhibition rate with increase in concentration of the extract from 625 to 5000 μg/ml. Methanol extract had the highest inhibition rate in all concentration from 625 to 5000 μg/ml. Concerning larval mortality, HWE had the highest effect in L1 larval mortality in all concentration above 1250 μg/ml. Distilled water had very little larval mortality on L1 and L2 larvae by 3.3 and 3.3% respectively. Conclusion: This in vitro study brought out the ovicidal and larvicidal properties of stem bark of Terminalia glaucescens.

关键词： Anthelmintic Extract Haemonchus contortus Ovicidal and larvicidal Activities Terminalia glaucescens

Anthelmintic activity of Securidaca longepedunculata(Family:Polygalaceae)root extract in mice,in vitro and in vivo

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Asian Pacific Journal of Tropical Medicine 2013年第11期6卷 841-846页

作者： Adiele RC Fakae BB Isuzu IU Department of Anatomy and Cell Biology.Division of Biomedical Sciences College of MedicineUniversity of SaskatchewanSaskatoon.SK.S7N5E Cameco MS Neuroscience Research Center Saskatoon City HospitalSaskatoonSKS7K OM7 Department of Veterinary Parasitology and Entomology Faculty of Veterinary MedicineUniversity of NigeriaNsukka Vice Chancellor's Office River State university of Science and TechnologyPort Harcourt Department of Veterinary Physiology and Pharmacology Faculty of Veterinary MedicineUniversity of NigeriaNsukka Deputy Vice Chancellor's Office.University of Nigeria Nsukka

Objective:To elucidate the pharmacological bases of oral administration of Securidaca longepedunculata(S.longepedunculata) root extract as an anthelmintic in folkloric medicine. Methods:Albino mice were infected with infective third(L3) larval stage of Heligmosomoides polygyrus(H.polygyrus) by esophageal intubation.Following establishment of the adult worms in the intestine,the mice were treated with 0-2 000 mg/kg body weight(bw) of methanolic root extract of S.longepedunculata and 100 mg/kg bw of pyrantel embonate,the reference drug in vivo. Bioactivity and larvicidal effects of the extract were tested by exposing brine shrimps(Artemia salina) to 0.00-1.00 mg/mL and the L3 stage of Heligmosomoides contortus(H.contortus) and H.polygyrus to 0.00-2.50 mg/mL of the extract in vitro.Results:The percentage yield of the extract was 7.13%w/w dry matter.The brine shrimps toxicity bioassay resulted in an LC_(50) of 74.18 μ g/mL.The extract had a significant,dose-dependent larvicidal effect on the L3 stage of H.contortus and H.polygyrus with the terminal effect of 75%and 70%at the highest exposure concentrations,respectively.The extract however,did not affect the number of worm eggs per gram(epg) of fecal materials(Plarvicidal effect on L3 stage of H.contortus and H.polygyrus,substantiating its use as anthelmintic in alternative medicine.

关键词： Anthelmintic Securidaca longepedunculata Haemonchus contortus Heligmosomoides polygyrus Brine shrimps larvicidal Fecal egg count

Evaluation of larvicidal activity of Pongamia pinnata extracts against three mosquito vectors

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Asian Pacific Journal of Tropical Biomedicine 2013年第11期3卷 853-858页

作者： Guna Ranjan Kolli Balakrishnan Vijayan Raja Sundararajan GITAM Institute of Pharmacy GITAM University National Centre for Disease Control(Formely Southern India Branch NICD) Department of Pharmaceutical Biotechnology J.S.S College of Pharmacy

Objective:To evaluate the mosquito larvicidal activity of Pongamia pinnata(P.pinnata)extracts against three mosquito vectors.Methods:The methanol and hydroalcohol extracts of bark part of P.pinnata L were tested against fourth instar larvae of Culex quinqnefasciatus,Aedes aegypti and Anopheles stephensi.The mortality was observed 24 h and 48 h after treatment,data was subjected to probit analysis to determine lethal concentration(LC_(50)and LC_(90))to kill 50 and 90 percent of treated larvae of tested species.Results:The larval mortality was found in both methanol and hydroalcohol extracts of P.pinnata against Culex quinquefascialus,Aedes aegypti and Anopheles stephensi with LC_(50)values of 84.8,118.2 and 151.7 ppm;97.7,128.3 and 513 ppm.The highest larval mortality was found in methanol extract of P.pinnala when comparable to the hydroalcohol extract.Conclusions:These results suggest that both methanol and hyrdoalcohol extracts have the potential to be used as an ideal ecofriendly approach for the control of disease vectors.This could lead to isolation of novel natural larvicidal compounds.

关键词： Pongamia pinnata L. larvicidal Mosquito vectors Culex quinquefasciatus Aedes aegypti Anopheles stephensi

Studies on effects of Andrographis paniculata(Burm.f.) and Andrographis lineata nees(Family:Acanthaceae) extracts against two mosquitoes Culex quinquefasciatus(Say.) and Aedes aegypti(Linn.)

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Asian Pacific Journal of Tropical Medicine 2013年第3期6卷 176-179页

作者： Renugadevi G Ramanathan T Shanmuga priya R Thirunavukkarasu P Centre of Advanced Study in Marine Biology Faculty of Marine SciencesAnnamalai UniversityParangipettai 608 502Tamil NaduIndia

Objective:To investigate the studies on effects of Andrographis paniculata(A.paniculata) (Burm.f.) and Andrographis lineata(A.lineata) nees(Family:Acanthaeeae)extracts against two mosquitoes Culex quinquefasciatus(Cx.quinquefasciatus)(Say.) and Aedes aegypti(Ae.aegypti) (Linn.).Methods:The aqueous and petroleum ether extracts of two plant species.A.paniculata and A.lineate were examined against the larvae of,4.aegypti(L.) and Cx.quinquefasciatus with gradually increasing concenlration ie.from 50 to 200 ppm of solvent extracts and to test their activity in combination with each other.Results:In a 24 h bioassay experiment with plant extracts,highest mortalities were recorded at 200 ppm of concentrations for leaves of 4.lineta and A.paniculata individually.For combination effect,only 150 ppm of the mixture of solvent extracts of petroleum ether:aqueous(1:1) extracts showed 100%mortality after 24 h of exposure. Conclusions:The results show that,insecticides of plant combination is ecofriend and has better larvicidal activity compared to individual extracts.

关键词： Andrographis paniculata Culex quinquefasciatus Petroleum ether larvicidal activity Combination effect Mosquitoes Chikungunya fever

Evaluation of Andrographis paniculata Burm.f.(Family.Acanthaceae) extracts against Culex quinquefasciatus(Say.) and Aedes aegypti(Linn.)(Diptera:Culicidae)

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Asian Pacific Journal of Tropical Medicine 2011年第3期4卷 176-181页

作者： Marimuthu Govindarajan Division of Vector Biology and Phytochemistry Department of ZoologyAnnamalai UniversityAnnamalainagar-608 002Tamil NaduIndia

Objective:To investigate the larvicidal and ovicidal efficacy of different extracts of Andrographis paniculata（A.paniculata） against Culex quinquefasciatus（Cx.quinquefasciatus） Say and Aedes aegypti（Ae.aegypti ） L（Diptera:Culicidae）.Methods:larvicidal efficacy of the crude leaf extracts of A.paniculata with five different solvents like benzene,hexane,ethyl acetate, methanol and chloroform was tested against the early third instar larvae of Cx.quinquefasciatus and Ae.aegypti.The ovicidal activity was determined against two mosquito species to various concentrations ranging from 50-300 ppm under the laboratory conditions.Results:The benzene, hexane,ethyl acetate,methanol and chloroform leaf extract of A.paniculata was found to be more effective against Cx.quinquefasciatus than Ae.aegypti.The LC50 values were 112.19,137.48, 118.67,102.05,91.20 ppm and 119.58,146.34,124.24,110.12,99.54 ppm respectively.Among five tested solvent,methanol and ethyl acetate crude extract was found to be most effective for ovicidal activity against two mosquito species.The extract of methanol and ethyl acetate exerted 100%mortality at 200 ppm against Cx.quinquefasciatus and at 250 ppm against Ae.aegypti. Conclusions:From the results it can be concluded the crude extract of A.paniculata was a potential for controlling Cx.quinquefasciatus and Ae.aegypti mosquitoes.

关键词： Andrographis paniculata larvicidal activity Ovicidal activity Culex quinquefasciatus Aedes aegypti

Synthesis and Bio-activities of Pyrazolyl or Pyridinyl Substituted Tonghaosu Analogs

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Chinese Journal of Chemistry 2007年第6期25卷 808-813页

作者：尹标林陈立徐汉虹张健存吴毓林 Guangzhou Institute of Biomedicine and Health Chinese Academy of Science Guangzhou Guangong 510663 China State Key Laboratory ofBio-organic & Natural Products Chemistry Shanghai Institute of Organic Chemistry Chinese Academy of Sciences Shanghai 200032 China Laboratory of Insect Toxicology South China Agricultural University Guangzhou Guangdong 510642 China

11 pyrazolyl or pyridinyl substituted tonghaosu analogs were synthesized. Structures of all the new compounds were confirmed by ^1H NMR, IR, MS, HREIMS or elemental analysis. Their antifeedant activity against larvae of large white butterfly （Pieris brassicae L.）, larvicidal activity toward mosquito （Culex quinquefasciatus Say） and growth control activity toward larvae of Spodoptera litura Fab were examined. Some of them exhibited antifeeding activities comparable to or stronger than tonghaosu Z-1. Based on the activity data, the preliminary structure-activity relationship was also discussed, which might be instructive for finding out lead compounds with better bioactivities in the future.

关键词： spiroketal tonghaosu analog antifeedant larvicidal growth control Pieris brassicae Culex quinquefasciatus Spodoptera litura

Mosquitocidal properties of Solanum trilobatum L.(Solanaceae) leaf extracts against,three important human vector mosquitoes(Diptera:Culicidae)

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Asian Pacific Journal of Tropical Medicine 2013年第11期6卷 854-858页

作者： Selvaraj Premalatha Kuppusamy Elumalai Alagarmalai Jeyasankar Department of Zoology & Botany Arignar Anna Government Arts CollegeMusiri-621 211 Tamil NaduIndia Unit of Enlomoloxicity Department of Advanced Zoology & BiotechnologyGovt.Arts College NandanamChennai -600 035Tamil NaduIndia

Objective:To determine the larvicidal and pupicidal activites of Solanum trilobatum(S. trilobatum) leaf extracts against Aedes aegypti(Ae.aegypti),Culex quinquefasciatus(Cx. quinquefasciatus) and Anopheles stephensi(An.stephensi).Methods:The larvicidal and pupucidal was determined at five different concentrations of 50,100,150,200 and 250 ppm.Percentage of larval mortality was assessed after 48 h.Results:Methanol extracts of S.trilobatum was found to be more susceptible against the larvae of Ae.aegypti.Cx.quinquefasciatus and An.stephensi at 250 ppm with a LC_(50) value of 125.43,127.77 and 116.64 ppm respectively.Leaf methanol extracts of S.trilobatum also exhibited pupicidal and adult emergence properties.Conclusions:These results suggested that the leaf extracts of S.Irilobalum showed potential to be used as an ideal ecofriendly approach for the control of the Ae.Aegypti,Cx.quinquefasciatus and An.stephensi.

关键词： Solanum trilobatum leaf extracts Aedes aegypti Culex quinquefasciatus Anopheles stephensi larvicidal

Effect of leaf essential oil of Coccinia indica on egg hatchability and different larval instars of malarial mosquito Anopheles stephensi

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Asian Pacific Journal of Tropical Medicine 2011年第12期4卷 948-951页

作者： Sankaran Rajkumar Arulsamy Jebanesan Rajarathinavelu Nagarajan Department of Zoology and Wildlife Biology AVC College - 609 305Tamil NaduIndia Division of Vector Biology Department of ZoologyAnnamalai UniversityAnnamalai Nagar-608 002Tamil Nadu.India Department of Zoology and Wildlife Biology AVC College - 609 305.Tamil NaduIndia

Objective:To assess the larvicidal and egg hatching inhibition property of the leaf essential oil of Cocrinia indica（C.indica） against Anopheles stephensi（An.stephensi）.Methods:The larvicidal potential of C.indica leaf essential oil was evaluated against 1st.2nd.3rd and 4th instars larvae of An.stephensi using WHO protocol.The 24h LC50and LC90 values of the essential oil were determined following probit analysis.The egg hatching inhibition activity was also tested at 10.***.40.and 60 mg/L The IC50 value of essential oil was determined against eggs of An. stephensi.Results:The essential oil extracted from C.indica possessed excellent larvicidal and egg hatching inhibition activity against An.stephensi.The bioassays showed LC50—LC90of 54.3- 140.***.65.5-155.6,86.8-180.7 and 95.3-192.6 for 1 st.***.3rd.and 4th larval insiars.respectively. The 5095 egg hatching inhibition concentration(IC50) was noted at 16.5 mg/L.Conclusions:The present finding suggest that the C.indica leaf essential oil provided an excellent potential for controlling An.slephemi mosquito at earlier stage of their life cycle.

关键词： Anopheles stephensi Coccinia indica Essential oil larvicidal activity Egg hatching inhibition