检索结果-南通市图书馆

Genome editing in large animals: current status and future prospects

National Science Review 2019年第3期6卷 402-420页

作者： Jianguo Zhao Liangxue Lai Weizhi Ji Qi Zhou State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of Sciences Savaid Medical School University of Chinese Academy of Sciences Institute of Stem Cell and Regeneration Chinese Academy of Sciences South China Institute for Stem Cell Biology and Regenerative Medicine Guangzhou Institutes of Biomedicine and Health Chinese Academy of Sciences Yunnan Key Laboratory of Primate Biomedicine Research Institute of Primate Translational Medicine Kunming University of Science and Technology CAS Center for Excellence in Brain Science and Intelligence Technology (CEBSIT)

large animals(non-human primates, livestock and dogs) are playing important roles in biomedical research, and large livestock animals serve as important sources of meat and milk. The recently developed programmable DNA nucleases have revolutionized the generation of gene-modified large animals that are used for biological and biomedical research. In this review, we briefly introduce the recent advances in nuclease-meditated gene editing tools, and we outline these editing tools’ applications in human disease modeling, regenerative medicine and agriculture. Additionally, we provide perspectives regarding the challenges and prospects of the new genome editing technology.

关键词： genome editing large animal disease model agriculture xenotransplantation

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

In vivo evaluation of additively manufacturedmulti-layered scaffold for the repair of large osteochondral defects

引用

Bio-Design and Manufacturing 2022年第3期5卷 481-496页

作者： Maryam Tamaddon Gordon Blunn Rongwei Tan Pan Yang Xiaodan Sun Shen-Mao Chen Jiajun Luo Ziyu Liu Ling Wang Dichen Li Ricardo Donate Mario Monzón Chaozong Liu Institute of Orthopaedic and Musculoskeletal Science Royal National Orthopaedic HospitalUniversity College LondonStanmore HA74LPUK School of Pharmacy and Biomedical Sciences University of PortsmouthPortsmouth PO12DTUK Guangdong Engineering Research Center of Implantable Medical Polymer Shenzhen Lando Biomaterials Co.Ltd.Shenzhen 518107China School of Materials Science and Engineering Tsinghua UniversityBeijing 100084China State Key Laboratory for Manufacturing System Engineering School of Mechanical EngineeringXi’an Jiaotong UniversityXi’an 710054China Departamento de Ingeniería Mecánica Grupo de Investigación en Fabricación Integrada y AvanzadaUniversidad de Las Palmas de Gran CanariaCampus Universitario de Tafira s/n35017 Las PalmasSpain

The repair of osteochondral defects is one of the major clinical challenges in ***-established osteochondral tissue engineering methods have shown promising results for the early treatment of small ***,less success has been achieved for the regeneration of large defects,which is mainly due to the mechanical environment of the joint and the heterogeneous nature of the *** this study,we developed a multi-layered osteochondral scaffold to match the heterogeneous nature of osteochondral tissue by harnessing additive manufacturing technologies and combining the established art laser sintering and material extrusion *** developed scaffold is based on a titanium and polylactic acid matrix-reinforced collagen“sandwich”composite *** microstructure and mechanical properties of the scaffold were examined,and its safety and efficacy in the repair of large osteochondral defects were tested in an ovine condyle *** 12-week in vivo evaluation period revealed extensive and significantly higher bone in-growth in the multi-layered scaffold compared with the collagen–HAp scaffold,and the achieved stable mechanical fixation provided strong support to the healing of the overlying cartilage,as demonstrated by hyaline-like cartilage *** histological examination showed that the regenerated cartilage in the multi-layer scaffold group was superior to that formed in the control *** genes such as aggrecan and collagen-II were upregulated in the scaffold and were higher than those in the control *** findings showed the safety and efficacy of the cell-free“translation-ready”osteochondral scaffold,which has the potential to be used in a one-step surgical procedure for the treatment of large osteochondral defects.

关键词： Osteochondral scaffold large animal Additive manufacturing Porous titanium

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：