In vivo evaluation of additively manufacturedmulti-layered scaffold for the repair of large osteochondral defects

作     者：Maryam Tamaddon Gordon Blunn Rongwei Tan Pan Yang Xiaodan Sun Shen-Mao Chen Jiajun Luo Ziyu Liu Ling Wang Dichen Li Ricardo Donate Mario Monzón Chaozong Liu Maryam Tamaddon;Gordon Blunn;Rongwei Tan;Pan Yang;Xiaodan Sun;Shen-Mao Chen;Jiajun Luo;Ziyu Liu;Ling Wang;Dichen Li;Ricardo Donate;Mario Monzón;Chaozong Liu

作者机构：Institute of Orthopaedic and Musculoskeletal ScienceRoyal National Orthopaedic HospitalUniversity College LondonStanmore HA74LPUK School of Pharmacy and Biomedical SciencesUniversity of PortsmouthPortsmouth PO12DTUK Guangdong Engineering Research Center of Implantable Medical PolymerShenzhen Lando Biomaterials Co.Ltd.Shenzhen 518107China School of Materials Science and EngineeringTsinghua UniversityBeijing 100084China State Key Laboratory for Manufacturing System EngineeringSchool of Mechanical EngineeringXi’an Jiaotong UniversityXi’an 710054China Departamento de Ingeniería MecánicaGrupo de Investigación en Fabricación Integrada y AvanzadaUniversidad de Las Palmas de Gran CanariaCampus Universitario de Tafira s/n35017 Las PalmasSpain 

出 版 物：《Bio-Design and Manufacturing》 (生物设计与制造（英文）)

年 卷 期：2022年第5卷第3期

页      面：481-496页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 0836[工学-生物工程] 

基　　金：financially supported by the Versus Arthritis (No. 21160) the Rosetree Trust (No. A1184) the European Commission via H2020-MSCA-RISE Program (BAMOS Project (No.734156)) Innovate UK via Newton Fund (No. 102872) the Engineering and Physical Science Research Council (EPSRC) via DTP Case Programme (No. EP/T517793/1) 

主　　题：Osteochondral scaffold Large animal Additive manufacturing Porous titanium 

摘      要：The repair of osteochondral defects is one of the major clinical challenges in ***-established osteochondral tissue engineering methods have shown promising results for the early treatment of small ***,less success has been achieved for the regeneration of large defects,which is mainly due to the mechanical environment of the joint and the heterogeneous nature of the *** this study,we developed a multi-layered osteochondral scaffold to match the heterogeneous nature of osteochondral tissue by harnessing additive manufacturing technologies and combining the established art laser sintering and material extrusion *** developed scaffold is based on a titanium and polylactic acid matrix-reinforced collagen“sandwichcomposite *** microstructure and mechanical properties of the scaffold were examined,and its safety and efficacy in the repair of large osteochondral defects were tested in an ovine condyle *** 12-week in vivo evaluation period revealed extensive and significantly higher bone in-growth in the multi-layered scaffold compared with the collagen–HAp scaffold,and the achieved stable mechanical fixation provided strong support to the healing of the overlying cartilage,as demonstrated by hyaline-like cartilage *** histological examination showed that the regenerated cartilage in the multi-layer scaffold group was superior to that formed in the control *** genes such as aggrecan and collagen-II were upregulated in the scaffold and were higher than those in the control *** findings showed the safety and efficacy of the cell-free“translation-readyosteochondral scaffold,which has the potential to be used in a one-step surgical procedure for the treatment of large osteochondral defects.