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Exopolysaccharides of lactobacillus rhamnosus GG ameliorate Salmonella typhimurium-induced intestinal inflammation via the TLR4/NF-κB/MAPK pathway

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Journal of Animal Science and Biotechnology 2023年第4期14卷 1501-1513页

作者： Jinze Li Qiuke Li Qianhui Wu Nan Gao Zhihua Wang Yang Yang Anshan Shan Institute of Animal Nutrition Northeast Agricultural UniversityHarbin 150030P.R.China

Background Salmonella typhimurium(S.T),as an important foodborne bacterial pathogen,can cause diarrhea and gastroenteritis in humans and *** studies have confirmed that exopolysaccharides(EPSs)have various biological functions,but the mechanism through which EPSs improve the immunity of animals against the invasion of pathogenic bacteria is ***,we explored the protective effect of EPSs of lactobacillus rhamnosus GG(LGG)on the S.T-infected *** Mice received adequate food and drinking water for one week before the start of the *** 7 d of prefeeding,2×108 CFU/mL S.T solution and an equivalent volume of saline(control group)were given orally for 1 *** the fourth day,the mice were treated with 0.5 mg/mL EPSs,1.0 mg/mL EPSs,2.0 mg/mL EPSs,or 2.0 mg/mL penicillin for 7 ***,the body and relative organ weight,histological staining,and the levels of antioxidant enzyme activity and inflammatory cytokines were *** The S.T-infected mice exhibited symptoms of decreased appetite,somnolence,diarrhea and flagging *** with EPSs and penicillin improved the weight loss of the mice,and the high dose of EPSs showed the best therapeutic *** significantly ameliorated S.T-induced ileal injury in ***-dose EPSs were more effective than penicillin for alleviating ileal oxidative damage induced by *** mRNA levels of inflammatory cytokines in the ileum of mice showed that the regulatory effects of EPSs on inflammatory cytokines were better than those of *** could inhibit the expression and activation of key proteins of the TLR4/NF-κB/MAPK pathway and thereby suppress the level of S.T-induced ileal *** EPSs attenuate S.T-induced immune responses by inhibiting the expression of key proteins in the TLR4/NF-κB/MAPK signaling ***,EPSs could promote bacterial aggregation into clusters,which may be a potential strategy for reducing the bacterial invasion of

关键词： Exopolysaccharides lactobacillus rhamnosus Salmonella typhimurium TLR4/NF-κB/MAPK

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Lactic Acid Fermentation from Coffee Ground Waste Hydrolysate by lactobacillus rhamnosus

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Journal of Renewable Materials 2019年第4期7卷 365-372页

作者： Ja-Ryong Koo Hye Min Park Se Kyung Kim Hyun Shik Yun Department of Biological Engineering Inha UniversityIncheon 22212Korea Institute of Industrial Biotechnology Inha University Research and Business FoundationIncheon 22212Korea

Lactic acid is an important organic acid that is widely used in the food,pharmaceutical,and cosmetic *** acid was produced from coffee ground waste which contains fermentable sugars and is increasingly generated from our daily dietary *** 114 strains of lactobacillus species,lactobacillus rhamnosus ATCC 10863 was selected for the production of lactic acid from coffee ground *** alkali pretreatment and saccharification,cellulose and hemicellulose in coffee ground waste were converted into fermentable *** experiments were conducted at various alkali solution,concentrations,and *** pretreatment with 35 g/L of KOH at 121oC for 60 min,the highest concentration of fermentable sugars was *** optimum concentration of Viscozyme L was 2%when saccharification was proceeded at 55oC for 7 *** productivity of lactic acid fermentation was the highest(0.59 g/L/h)at 100 g of coffee ground waste(1x concentration),whereas the lactic acid concentration was the highest at 600 g of coffee ground waste(6x concentration).As the concentration of coffee ground increased,the lactic acid concentration was also increased,however,the amount was not proportional to the coffee ground waste *** this study,it was found that coffee ground waste could be used as a culture medium for lactobacillus rhamnosus ATCC 10863 through pretreatment and saccharification for the production of lactic acid.

关键词： Coffee ground waste lactobacillus rhamnosus lactic acid fermentable sugars saccharification alkali pretreatment

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Effect of fructooligosaccharides on the colonization of lactobacillus rhamnosus AS 1.2466^(T) in the gut of mice

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Food Science and Human Wellness 2023年第2期12卷 607-613页

作者： Zhihua Niu Meijuan Zou Tingting Bei Na Zhang Dongyao Li Miaoshu Wang Chen Li Hongtao Tian College of Food Science and Technology Hebei Agricultural UniversityBaoding 071000China College of Biochemistry and Environmental Engineering Baoding UniversityBaoding 071000China New Hope Tensun(Hebei)Dairy Co. LtdBaoding 071000China Hebei Technology Innovation Center of Probiotic Functional Dairy Product Baoding 071000China National Engineering Research Center for Agriculture in Northern Mountainous Areas Baoding 071000China

lactobacillus rhamnosus and fructooligosaccharides(FOS)have been widely studied so ***,the effects of *** on the intestinal microecological environment at the species level and the effect of different proportions of FOS on *** colonization in different parts of mice intestine are still *** study results indicated that the specific bands of enterobacterial repetitive intergenic consensus polymerase chain reaction(ERIC-PCR)in the ***(LR)group significantly increased at 7 *** the number of bands was similar to the natural recovery(NR)group,the brightness of few bands significantly enhanced in the later stage of ***,Southern-blot maps showed strong signals,indicating that the ERIC-PCR fingerprint could accurately reflect the changes in the mouse gut microbiota ***,the high-throughput results confirmed that the lactobacillus and Akkermansia had different changes at different periods,but all of them showed an upward trend,while the Klebsiella were inhibited,thereby maintaining the intestinal microecology ***,FOS exerted a positive effect on *** colonization in the gut.

关键词： Intestinal microbiota Synbiotics Fructooligosaccharides lactobacillus rhamnosus

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A cellular chip-MS system for investigation of lactobacillus rhamnosus GG and irinotecan synergistic effects on colorectal cancer

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Chinese Chemical Letters 2022年第4期33卷 2096-2100页

作者： Wanting Hu Dan Gao Zhaochen Su Rui Qian Yu Wang Qionglin Liang State Key Laboratory of Chemical Oncogenomics the Graduate School at ShenzhenTsinghua UniversityShenzhen 518055China Department of Chemistry Center for Synthetic and Systems BiologyKey Laboratory of Bioorganic Phosphorus Chemistry&Chemical Biology(Ministry of Education)Tsinghua UniversityBeijing 100084China

Colorectal cancer(CRC) is still the leading cause of cancer death worldwide, but the clinical effect of drug therapy such as irinotecan is not an ideal way at present. In recent years, probiotics have attracted much attention, and the combination of probiotics may play an important role in the prevention and treatment of CRC. This work proposed a cellular chip-MS system, to study the synergistic effects of probiotic lactobacillus rhamnosus GG(***) and irinotecan on HCT116 cells by cell viability and on-line mass spectrometry(MS) analysis. The double-layer chip sandwiched with a polycarbonate membrane can coculture HCT116 cells and ***. And the solid phase microextraction chip can be used for desalination and concentration. Finally, the extracted chemicals were entered the electrospray ionization quadrupole time-of-flight MS to detect irinotecan metabolites. The results showed that with the increasing concentration of co-cultured ***, the percentage of living HCT116 cells decreased, but the relative amount of metabolized SN-38 by HCT116 cells increased. Therefore, the microfluidic system can be used to detect and monitor the synergistic effect of *** combination on HCT116 cells. In summary, our study provided experimental evidence for the first time with potential applications of *** combination in CRC treatment, and the cellular chip-MS system as a powerful tool can be used in the experiments of probiotics as new drugs.

关键词： Irinotecan lactobacillus rhamnosus Microfluidics Mass spectrometry Colorectal cancer

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Amelioration of dextran sodium sulphate-induced colitis in mice by treatment with lactobacillus rhamnosus and lactobacillus reuteri:intraspecific and interspecific patterns

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Food Science and Human Wellness 2024年第5期13卷 2799-2812页

作者： Chunxiu Lin Yuxing Zheng Bo Zhang Guopeng Lin Kexin Shang Jianxin Zhao Gang Wang Wei Chen State Key Laboratory of Food Science and Resources Jiangnan UniversityWuxi 214122China School of Food Science and Technology Jiangnan UniversityWuxi 214122China National Engineering Research Center for Functional Food Jiangnan UniversityWuxi 214122China (Yangzhou)Institute of Food Biotechnology Jiangnan UniversityYangzhou 225004China

lactobacillus rhamnosus(Rh)and lactobacillus reuteri(Re)are well-known probiotic species in inflammatory bowel disease(IBD)*** variations between these species’efficacy against colitis,and their model of action in this regard,are intriguing and enable treatment to be individually tailored to *** this study,four strains each of Rh and Re were isolated from fecal samples and their draft genomes were *** anti-colitis activities of both strains involved various aspects of intestinal immune,physical,chemical,and biological barrier ***,the tested strains exhibited considerable interspecies and intraspecies specificity in colitis *** strains significantly outperformed Re strains in terms of short-chain fatty acid ***,Re strains were more effective than Rh strains in inhibiting production of inflammatory factors;promoting production of intestinal mucus,antimicrobial peptides,and tight junction proteins;and supporting the stem cell *** accounts for the anti-colitis outcomes of Re strains being superior to those of Rh *** addition,the effective Rh and Re strains were found to express high concentrations of specific carbohydrate metabolism-and prophage-related genes,*** together,the results of this study could assist researchers in developing effective therapies for IBD.

关键词： Inflammatory bowel disease Anti-colitis lactobacillus rhamnosus lactobacillus reuteri Intestinal barrier function Short-chain fatty acids

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Oral administration of lactobacillus rhamnosus GG to newborn piglets augments gut barrier function in pre-weaning piglets

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2019年第2期20卷 180-192页

作者： Yang WANG Li GONG Yan-ping WU Zhi-wen CUI Yong-qiang WANG Yi HUANG Xiao-ping ZHANG Wei-fen LI Key Laboratory of Molecular Animal Nutrition and Feed Science Key Laboratory of Animal Feed and Nutrition of Zhejiang ProvinceCollege of Animal ScienceZhejiang UniversityHangzhou 310058China Department of Animal Sciences Oregon State UniversityCorvallisOR 97330USA College of Animal Science and Technology Guangxi UniversityNanning 530005China China National Bamboo Research Center Key Laboratory of High Efficient Processing of Bamboo of Zhejiang ProvinceHangzhou 310012China

To understand the effects of lactobacillus rhamnosus GG(ATCC 53103)on intestinal barrier function in pre-weaning piglets under normal conditions,twenty-four newborn littermate piglets were randomly divided into two *** in the control group were orally administered with 2 mL 0.1 g/mL sterilized skim milk while the treatment group was administered the same volume of sterilized skim milk with the addition of viable *** at the 1st,3rd,and 5th days after *** feeding trial was conducted for 25 *** showed that piglets in the *** group exhibited increased weaning weight and average daily weight gain,whereas diarrhea incidence was *** bacterial abundance and composition of cecal contents,especially Firmicutes,Bacteroidetes,and Fusobacteria,were altered by probiotic *** addition,*** increased the jejunal permeability and promoted the immunologic barrier through regulating antimicrobial peptides,cytokines,and chemokines via Toll-like *** findings indicate that oral administration of *** GG to newborn piglets is beneficial for intestinal health of pre-weaning piglets by improving the biological,physical,and immunologic barriers of intestinal mucosa.

关键词： lactobacillus rhamnosus Gut microbiota Intestinal physical barrier Intestinal immunological barrier Piglet

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Soothing Efficacy and Tolerability of a Skin Care Product Containing Live lactobacillus rhamnosus Bacteria and Berry Seed Oils on Atopic Dermatitis Lesions

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Journal of Cosmetics, Dermatological Sciences and Applications 2019年第2期9卷 83-93页

作者： Beata Imko-Walczuk Aleksandra Taraszkiewicz Annika Mayra Dermscan Poland GdanskPoland Gutguide Ltd. HelsinkiFinland

Background: Atopic dermatitis (AD) is a chronic pruritic inflammatory skin disease characterized by a reduced microbiome diversity which favours the skin colonization with pathogenic bacteria, mainly with Staphylococcus aureus. A novel approach to the management of several skin diseases, including AD, involves the use of specific beneficial probiotic bacteria aimed at restoring the skin microbiome balance and decreasing the skin colonization with pathogens. Objective: This study assessed the soothing efficacy and tolerability of a new probiotic-based skin care product combining two live lactobacillus rhamnosus strains and berry seed oils on eczematous lesions in subjects with atopic skin. Patients and Methods: Twenty-five adults with atopic skin and presenting at least one inflammatory lesion applied the product on the defined lesion for 28 days, once to twice daily. Severity of eczema signs (EASI score) and intensity of pruritus (VAS) were assessed, and digital images were captured following treatment. Cutaneous tolerability and subject satisfaction were also investigated. Results: A statistically significant decrease in mean EASI score was observed after 28 days (-27%, p = 0.011). A visible improvement in skin aspect was observed in 67% of the subjects on week 4. On average, the intensity of pruritus decreased by 57% as compared to baseline (p = 0.011). This effect was observed in 86% of the subjects. No relevant adverse reactions were observed following treatment with the skin care product and the majority of the subjects appreciated the product for its general characteristics, properties, and efficacy. Conclusion: The results suggest that this skin care product containing two live lactobacillus rhamnosus strains and berry seed oils could be used for the treatment of eczematous lesions in subjects with atopic skin.

关键词： Atopic Dermatitis lactobacillus rhamnosus Probiotic Berry Seed Oils Pruritus

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lactobacillus intestinalis/lactobacillus rhamnosus protects against AFB_(1)-induced liver damage:involvement of intestinal mucosal barrier

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One Health Advances 2023年第1期1卷 94-105页

作者： Yuanyuan Chen Honglin Liu Tianhui An Qian Wu Hongtao Zhang Juan JLoor Jiaxin Cheng Junqi Wang Jian Sun Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases College of Animal Science and Veterinary MedicineHeilongjiang Bayi Agricultural UniversityNo.5 Xinyang RoadDaqingHeilongjiang 163319China Department of Animal Sciences and Division of Nutritional Sciences University of IllinoisUrbanaIL 61802USA Tonghua Dongchang Animal Disease Control Centre Dongchang DistrictTonghuaJilin 134001China

Aflatoxin B1(AFB1)is a widely spread mycotoxin that poses a threat to the healthy to human and *** liver is the main target organ for AFB1-induced damage,primarily causing inflammatory injury and oxidative *** AFB1 enters the body,it can damage the intestinal barrier function,and its metabolites are transported to the ***,the damage to the liver is closely associated with intestinal barrier *** plays a crucial role in mitigating liver damage by improving the intestinal barrier *** our previous report,we reported that lactobacillus reduces liver damage caused by ***,it is still unclear how the intestinal barrier contributes to the protective effects of lactobacillus against *** investigate the protective effects and intestinal barrier mechanisms of lactobacillus intestinals/rhamnosus against AFB1-induced liver damage,we orally administered AFB1 and lactobacillus intestinals/rhamnosus to male SD *** the body weight,organ index,histopathological changes in the liver and gut,liver and kidney function indicators,intestinal mucosal barrier indicators,serum AFB1 content and inflammatory factors,liver oxidative stress index,and short-chain fatty acids content were *** findings demonstrate that exposure to AFB1 resulted in changes in liver histopathology and biochemical functions,altered inflammatory response and oxidative stress,compromised the intestinal mucosal barrier,and induced the accumulation of inflammatory factor and inflammation in the ***,supplementation with lactobacillus intestinals or lactobacillus rhamnosus significantly prevented AFB1-induced liver injury,alleviated histopathological changes and hepatic injury by the maintenance of intestinal mucosal barrier integrity.

关键词： lactobacillus intestinalis lactobacillus rhamnosus AFB1 Liver damage Intestinal mucosal barrier

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Peptidoglycan derived from lactobacillus rhamnosus promotes antimicrobial peptides gene expression in chicken peripheral blood monocytes

Peptidoglycan derived from Lactobacillus rhamnosus promotes ...

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第十一届全国免疫学学术大会

作者： Li Guanhong Ai Hongying Peng Fuqiang Li Qiufen Peng Chengying Qu Mingren Jiangxi Province Key Laboratory of Animal Nutrition/College of Animal Sciene and Technology Jiangxi Agricultural University

Defensins and cathelicidins are the two major families of antimicrobial peptides found in animals, and play an important role in immune defense against infection. The peptidoglycan extracted from probiotic lactobacillus rhamnosus, plays an important role in immune regulation especially in promoting the expression of antimicrobial peptides. However, the mechanisms of action by which peptidoglycan promotes the expression of antimicrobial peptides has not yet been fully elucidated. The effects of whole peptidoglycan(WPG) derived from lactobacillus rnamnosus MLGA on avain β-defensin 9(AvB D9) and cathelicidin-B1(Cath-B1) gene expressions in peripheral blood monocytes(PBM) of chicken and the signaling pathway involved in induction of AvBD9 and Cath-B1 expressions by WPG were investigated in the present study. The quan-titative RT-PCR analysis was used to determine the expressions of related genes. Anti-TLR2 antibody, specific inhibitors of molecules in the signaling pathway were used to elucidate the signaling pathway involved in induction of AvBD9 and Cath-B1 by WPG WPG derived from lactobacillus rhamnosus MIGA dose-dependently promoted AvBD9 and Cath-B1 mRNA expressions in chicken PBM, where a peak response occurred at 200 μg/ml in AvBD9 induction and at 150μg/ml in Cath-B1 induction, respectively. WPG significantly upregulated Toll-like receptor 2(TLR2), NALP3 and NOD-like receptors 1(NOD1) mRNA expression in chicken PBM. AvBD9 and Cath-B1 expression were suppressed and the ratio of phosphorylated NF-κB, p38 MAPK, JNK proteins to respective non-phosphorylated ones were significantly decreased when TLR2 was blocked by anti-TLR2 specific antibody or NOD1 was inhibited by specific inhibitor. AvBD9 and CathB1 induction in chicken PBM by WPG was reduced by pretreatment with NF-κB inhibitor, or JNK inhibitor, or p38 MAPK *** conclusion, lactobacillus rhamnosus MLGA-derived WPG induces AvBD9 and Cath-B1 gene expressions in chicken peripheral blood monocytes was medi

关键词： lactobacillus rhamnosus peptidoglycan chicken peripheral blood monocytes antimicrobial peptides

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lactobacillus rhamnosus GR-1 attenuates Escherichia coli- induced inflammation and cell damage in primary bovine mammary epithelial cells

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畜牧与饲料科学 2015年第Z1期 343-343页

作者： WU Qiong ZHANG Wei LIU Mingchao ZHU Yaohong Department of Veterinary Clinical Sciences College of Veterinary MedicineChina Agricultural University

Bovine mammary epithelial cells(BMECs) were successfully cultivated using tissue mass inoculation method in this study,and fibroblast cells was removed by adding nutrition factors and controlling the time of *** isolated BMECs were thoroughly morphological and structural characteristic via immunocytochemistry,morphology,scanning electron microscopy(SEM) and transmission electron microscope(TEM).Our results showed that the obtained BMECs morphology together with the positive staining for Cytokeratin 18 was a powerful result to prove the specific epithelium characters of *** obtained BMECs were present as acobblestone form and proliferating *** cells had normal morphology,growth characteristics and mitochondrial and endoplasmic reticulum in cell cytoplasms,BMECs could be passaged 15 generations with a good behavior of proliferation,thus,it might represent an useful tool for studying the function of bovine mammary *** coli(***) is the major pathogens causing mammary infection of dairy *** are extensively used to promote gastrointestinal health,and emerging evidence suggests that their beneficial properties can extend beyond the local environment of the ***,we determined whether pre-incubated with probiotics can protect against ***-induced *** medium alone,the BMECs were plump and with plenty of microvilli,whereas *** infection caused microvilli missing,cellular atrophy and different degrees of cell *** cells infected with *** alone,*** adhered on the surface of BMECs and the *** internalization in nucleus was *** BMECs incubated with only *** GR-1 maintained the intact ultrastructure and were with microvilli and plump ***,pretreatment with *** GR-1 prevented disruption of the ultrastructure of BMECs for up to 6 h following *** *** medium alone,the BMECs exhibited normal appearance(the cells with intact cytoplasm and organelle

关键词： lactobacillus rhamnosus Escherichia coli bovine mammary epithelial cell

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