检索结果-南通市图书馆

男性迟发性性腺功能减退症中西医结合研究展望

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国中西医结合杂志 2022年第11期42卷 1288-1290页

作者：何清湖湖南医药学院湖南418000 湖南中医药大学长沙410208

男性迟发性性腺功能减退症(late-onset hypogonadism in male,LOH)是部分中老年男性以血清睾酮水平降低,伴自主神经功能紊乱,精神心理障碍和性功能改变为主体症状的一组症候群^([1])。随着我国人口老龄化进程以及老年医学的发展,LOH逐... 详细信息

男性迟发性性腺功能减退症(late-onset hypogonadism in male,LOH)是部分中老年男性以血清睾酮水平降低,伴自主神经功能紊乱,精神心理障碍和性功能改变为主体症状的一组症候群^([1])。随着我国人口老龄化进程以及老年医学的发展,LOH逐渐受到重视,睾酮替代疗法(testosterone replacement therapy,TRT)是治疗LOH的主要方法.

关键词：中西医结合研究中老年男性精神心理障碍血清睾酮水平自主神经功能紊乱男性迟发性性腺功能减退症老年医学 LOH

Lost expression of thyroid hormone receptor-β1 mRNA in esophageal cancer

在线全文

学校读者我要写书评

暂无评论

Frontiers in Biology 2012年第4期7卷 368-373页

作者： Hao LI Xiao-Chun XU Department of Clinical Cancer Prevention UTM. D. Anderson Cancer Center Houston TX 77030 USA Department of Pathology Anhui Medical University Hefei 230032 China

Thyroid hormone receptors （TR）, ligand-mediated transcription factors, regulate cell growth, differentiation, and apoptosis. In humans, two different genes encode TR-α and TR-β and they are often co-expressed in various tissues at different levels. To explore the role of TR in esophageal cancer, we analyzed expression of TR-β1 mRNA （most abundantly expressed in the majority of normal cells） in normal and malignant esophageal tissue specimens using in situ hybridization. The TR-β1 mRNA was detected in 92.3% （96 of 104） of normal esophageal mucosa, whereas TR-β1 mRNA was only detected in 55.8% （58 of 104 cases） of esophageal squamous cell carcinoma specimens （P〈 0.00001）. Expression of TR-β1 mRNA was associated with well-differentiated cancers （P〈 0.001）. Furthermore, we determined whether the loss of heterozygosity （LOH） in TR-β1 gene locus would be responsible for the lost TR-β1 expression. Analysis of 73 esophageal tissue specimens generated 39 informative cases, 17 of which showed LOH （43.6%） but only 9 of these 17 cases were correlated with lost TR-β1 expression. This study demonstrated that expression of TR-β1 mRNA was lost in esophageal cancer tissues, which may be due to multiple mechanisms.

关键词： esophageal cancer thyroid hormone receptor in situ hybridization LOH

胰岛素抵抗与男性功能性性腺功能减退相关性的研究进展

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国男科学杂志 2022年第4期36卷 96-100页

作者：冀远(综述) 王璟琦(审校) 山西医科大学第一临床医学院太原030001 山西医科大学第二医院泌尿外科太原030001

男性性腺功能减退症(hypothyroidism)是由于雄性激素缺乏、减少或不能发挥作用所导致的疾病,并伴有身体异常、认知和性功能障碍等症状,在男性中很常见。功能性性腺机能减退症(Functional hypogonadism,FH)被定义为继发于衰老和/或糖尿... 详细信息

男性性腺功能减退症(hypothyroidism)是由于雄性激素缺乏、减少或不能发挥作用所导致的疾病,并伴有身体异常、认知和性功能障碍等症状,在男性中很常见。功能性性腺机能减退症(Functional hypogonadism,FH)被定义为继发于衰老和/或糖尿病、肥胖和/或代谢综合征等共病伴有低血清睾酮水平(Testosterone,T)的一种疾病,包括迟发性性腺功能减退(Late-onset hypogonadism,LOH)以及肥胖相关男性性腺功能减退症等。

关键词：性腺功能减退血清睾酮水平性功能障碍男性性腺功能减退症胰岛素抵抗代谢综合征 LOH 肥胖

LOSS OF HETEROZYGOSITY ON CHROMOSOME 17p13.3 IN OVARIAN CANCER AND CERVICAL CANCER

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Cancer Research 1998年第4期10卷 28-31页

作者：张国玲杨慧娟许凯黎周瑾秦瑞娣陆明华 Department of Gynecology Shanghai Cancer Hospital Shanghai Medical University Shanghai Shanghai Cancer Institute Shanghai

Objective: To identify the loss of heterozygosity (LOH) on chromosome 17p13 3 in ovarian cancer and cervical cancer Methods: The frequency of LOH on chromosome 17p13 3 in DNA samples from 24 ovarian cancers, 9 cervical cancers, and 13 non malignant gynecological diseases were determined respectively, using Southern blot method with probe PYNZ 22 Results: LOH on 17p13 3 was found in 12 of 24 (50 0%) ovarian cancers (including a borderline mucinous cystadenoma), 4 of 9 (44 4%) cervical carcinomas, and 1 of 13 (7 7%) non malignant gynecological diseases, which was cervical intraepithelial neoplasm III (CIN III) ( PLOH on 17p13 3 is associated with ovarian cancer and cervical cancer, suggesting that detection of LOH on 17p13 3 may be helpful to understand the molecular pathogenesis of ovarian cancer and cervical cancer

关键词： Ovarian neoplasms Cervical neoplasms 17p13 3 LOH

联合单核苷酸多态性微阵列及短串联重复序列技术产前诊断Beckwith-Wiedemann综合征胎儿1例

在线全文

学校读者我要写书评

暂无评论

现代妇产科进展 2023年第4期32卷 319-320页

作者：李燕青江矞颖王元白庄建龙福建省泉州市妇幼保健院·儿童医院产前诊断中心 362000

单核苷酸多态性微阵列(single nucleotide polymorphism array,SNP-array)除了能检出CNV外,还能检测出大多数单亲二倍体(uniparental disomy,UPD)和杂合性缺失(LOH)。本研究利用SNP-array及短串联重复序列技术(short tandem repeat,STR... 详细信息

关键词： Beckwith-Wiedemann LOH 短串联重复序列技术 SNP-array

Characterization of six tumorsuppressor genes and microsatellite instability in hepatocellular carcinomain southern African blacks

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 1999年第6期5卷 470-476页

作者： Martins C Kedda MA Kew MC MRC/CANSA/University Molecular Hepatology Research Unit Department of Medicine University of the Witwatersrand Medical School 7 York Road Parktown 2193 Johannesburg South Africa

AIM To analyse cumulative loss of heterozygosity (LOH) of chromosomal regions and tumor suppressor genes in hepatocellular carcinomas (HCCs) from 20 southern African blacks. METHODS p53, RB1, BRCA1, BRCA2, WT1 and E cadherin genes were analysed for LOH, and p53 gene was also analysed for the codon 249 mutation, in tumor and adjacent non tumorous liver tissues using molecular techniques and 10 polymorphic microsatellite markers. RESULTS p53 codon 249 mutation was found in 25% of the subjects, as was expected, because many patients were from Mozambique, a country with high aflatoxin B 1 exposure. LOH was found at the RB1, BRCA2 and WT1 loci in 20%(4/*!20) of the HCCs, supporting a possible role of these genes in HCC. No LOH was evident in any of the remaining genes. Reports of mutations of p53 and RB1 genes in combination, described in other populations, were not confirmed in this study. Change in microsatellite repeat number was noted at 9/*!10 microsatellite loci in different HCCs, and changes at two or more loci were detected in 15%(3/*!20) of subjects. CONCLUSION We propose that microsatellite/genomic instability may play a role in the pathogenesis of a subset of HCCs in black Africans.

关键词： carcinoma, hepatocellular southern African blacks cumulative LOH tumor suppressor genes microsatellite genomic instability liver neoplasms

Loss of heterozygosity on 10q23.3 and mutation of tumor suppressor gene PTEN in gastric cancer and precancerous lesions

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2005年第2期11卷 285-288页

作者： Yi-LingLi ZhongTian Dong-YingWu Bao-YuFu YahXin DepartmentofGastroenterology FirstHospitalofChinaMedicalUniversitySh_enyang110001LiaoningProvinceofChina DepartmentofSurgery SecondHospitalofChinaMedicalUniversityShenyang110003LiaoningProvinceChina TheFourthLaboratory CancerInstituteFirstHospitalofChinaMedicalUniversityShenyang110001LiaoningProvinceChina

AIM: To investigate the loss of heterozygosity (LOH) and mutation of tumor suppressor gene PTEN in gastric cancer and precancerous lesions.METHODS: Thirty cases of normal gastric mucosa, advanced and early stage gastric cancer, intestinal metaplasia, atrophic gastritis, and atypical hyperplasia were analyzed for PTEN LOH and mutations within the entire coding region of PTEN gene by PCR-SSCP denaturing PAGE gel electrophoresis,and PTEN mutation was detected by PCR-SSCP sequencing followed by silver staining.RESULTS: LOH rate found in respectively atrophic gastritis was 10% (3/30), intestinal metaplasia 10% (3/30), atypical hyperpiasia 13.3% (4/30), early stage gastric cancer 20%(6/30), and advanced stage gastric cancer 33.3% (9/30),None of the precancerous lesions and early stage gastric cancer showed PTEN mutations, but 10% (3/30) of the advanced stage gastric cancers, which were all positive for LOH, showed PTEN mutation.CONCLUSION: LOH of PTEN gene appears in precancerous lesions, and PTEN mutations are restricted to advanced gastric cancer, LOH and mutation of PTEN gene are closely related to the infiltration and metastasis of gastric cancer.

关键词：异型结合性 10q23.3 基因突变肿瘤抑制基因 PTEN 胃癌癌症前期损害消化系统 LOH

卵巢上皮性癌nm23H1基因遗传不稳定性与临床病理特性的关系

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

癌症 2006年第6期25卷 713-717页

作者：杨月琴李继承浙江大学细胞生物学研究所浙江杭州310031

背景与目的:基因的遗传不稳定性,包括微卫星不稳定(microsatelliteinstablility,MSI)和杂合性缺失(lossofheterozygosity,LOH),是导致抑癌基因功能失调,引起肿瘤发生的重要因素。本实验研究中国人17号染色体D17S396位点MSI和LOH对卵巢... 详细信息

背景与目的:基因的遗传不稳定性,包括微卫星不稳定(microsatelliteinstablility,MSI)和杂合性缺失(lossofheterozygosity,LOH),是导致抑癌基因功能失调,引起肿瘤发生的重要因素。本实验研究中国人17号染色体D17S396位点MSI和LOH对卵巢上皮性癌nm23H1蛋白表达的影响,阐明nm23H1基因遗传不稳定性与卵巢上皮性癌临床病理特性的关系,为揭示nm23H1基因作用机制和肿瘤转移机制提供实验依据。方法:采用PCR-单链构象多态性(PCR-SSCP)和常规银染检测25例卵巢上皮性癌及相应的非癌组织D17S396位点的MS和LOH,采用EnVision免疫组织化学和Leica-Qwin计算机图像分析检测nm23H1蛋白表达。结果:25例卵巢上皮性癌中,D17S396位点MSI、LOH检出率和nm23H1蛋白阳性率分别为16.00%、24.00%和56.00%。LOH发生率在淋巴转移组(66.67%)高于无淋巴转移组(10.53%,PLOH阳性组中nm23H1蛋白阳性率为0.00%,显著低于LOH阴性组的73.68%(PLOH的发生可作为卵巢组织恶变的判断指标。nm23H1基因的MSI和LOH,通过相互独立的途径调控卵巢上皮癌的发生和转移,后者可抑制卵巢上皮性癌局部nm23H1的表达,并与卵巢上皮癌高淋巴结转移、预后差有关。

关键词：卵巢肿瘤/遗传学 nm23H1基因 MSI LOH 遗传不稳定性淋巴结转移预后

胃癌组织17p13.3杂合性丢失及p53蛋白异常表达的研究

在线全文

学校读者我要写书评

暂无评论

中华病理学杂志 1997年第3期26卷 134-136页

作者：王东旭房殿春罗元辉刘为纹王明荣第三军医大学西南医院消化科

目的了解染色体１７ｐ１３．３丢失及ｐ５３蛋白异常表达在胃癌发生发展中的作用。方法应用ＰＣＲ－ＲＦＬＰ法及免疫组化技术检测了５１例胃癌手术标本中１７ｐ１３．３杂合性丢失及ｐ５３蛋白异常表达。结果发现在３１例信息个体中１... 详细信息

目的了解染色体１７ｐ１３．３丢失及ｐ５３蛋白异常表达在胃癌发生发展中的作用。方法应用ＰＣＲ－ＲＦＬＰ法及免疫组化技术检测了５１例胃癌手术标本中１７ｐ１３．３杂合性丢失及ｐ５３蛋白异常表达。结果发现在３１例信息个体中１７ｐ１３．３杂合丢失１６例，占５１．６％（１６／３１）。ｐ５３蛋白染色阳性１９例，占３７．３％（１９／５１）。１７ｐ１３．３杂合丢失与ｐ５３蛋白表达间无显著相关。１７ｐ１３．３杂合丢失率和ｐ５３蛋白阳性率在肠型及胃型胃癌差异无显著性（Ｐ＞０．０５），与临床病理参数无显著相关。１例早期胃癌检出１７ｐ１３．３杂合丢失。结论染色体１７ｐ１３．３杂合丢失、ｐ５３蛋白异常表达是胃癌的常见改变；１７ｐ１３．３杂合丢失可出现在胃癌的早期。

关键词：胃肿瘤染色体异常蛋白质 P53 LOH