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EFFECTS OF CHANGES IN PATTERN OF lhrh PULSE ON LH SECRETION BY PERFUSED ANTERIOR PITUITARY CELLS OF MALE RATS

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Science China Chemistry 1991年第2期34卷 161-170页

作者：屈智超喻晶华郭景郭建 Sichuan Family Planning Research Institute WHO Collaborating Centre for Research in Human Reproduction

In the present study, a perfusion system of dispersed cells was used to investigate theeffects of lhrh pulse amplitude and frequency, and lhrh continuous stimulation on LHsecretion by anterior pituitary cells of adult male rats. The results have shown that, in therange of lhrh concentrations from 1×10^(-10) to 1 ×10^(-6) mol/L, the dose-response curve of LHsecretion was linear. lhrh pulse frequency generated a biphasic LH response: increasinglhrh pulse frequency increased the basal LH secretion and decreased LH/pulse. When1 ×10^(-9), mol/L or greater lhrh was given at frequencies of 3 pulses/h or higher, it wasobserved that a maximal LH peak was induced and then the LH release declined progres-sively to its LH basal level, i.e. lhrh self-priming effect and LH desensitization occurred.Enhancement of amplitude of lhrh pulses could reduce pulse frequency required for prim-ing. Increases in frequency of lhrh pulses with high amplitude would provoke the prim-ing effect more quickly. In addition, continuous perfusion of lhrh with different con-centrations could also elicit the lhrh self-priming effect and LH desensitization. lhrhwith low concentration (1×10^(-10) mol/L) would take much longer to evoke a self-primingeffect. These results indicate that the LH secretion pattern is dependent on lhrh pulsatileamplitude and frequency, and will help to clarify the kinetics mechanisms by which LHpulses fluctuate in vivo.

关键词： lhrh pulses pituitary cells LH secretion

lhrh刺激雄性大鼠垂体LH分泌高峰时细胞形态变化的图象分析

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生理学报 1997年第1期49卷 88-94页

作者：周寿康赵伟毛全福唐威何志英顾敦瑜蒋文珏上海市计划生育科学研究所上海200032

应用ABC亲和组织化学顺序、MIS－I计算机图象处理系统测定外源性lhrh刺激雄性大鼠LH分泌高峰前后LH细胞面积、细胞内液泡面积和细胞形状等形态参数的变化。结果表明：雄性大鼠LH基础分泌时，LH细胞处于贮存状态：大细胞（＞340μm2）占L... 详细信息

应用ABC亲和组织化学顺序、MIS－I计算机图象处理系统测定外源性lhrh刺激雄性大鼠LH分泌高峰前后LH细胞面积、细胞内液泡面积和细胞形状等形态参数的变化。结果表明：雄性大鼠LH基础分泌时，LH细胞处于贮存状态：大细胞（＞340μm2）占LH阳性细胞56．7％，小细胞（＜190μm2）占2．2％，40％细胞内含有大量液泡，细胞形状大多偏向圆形。注射lhrh30min后，血中LH浓度显著升高，LH细胞平均面积明显减小；60min时，血中LH释放达到高峰，相应的形态学特征是大细胞降低至4％，小细胞增加到66％。随着细胞内液泡的大量消失，52．6％的细胞偏向不规则形状。LH分泌高峰后，血液LH浓度逐渐恢复到基础分泌水平，但随着细胞内LH贮存的增加，细胞和细胞内液泡平均面积逐渐增加，大多数细胞又偏向圆形。结果提示，合大量液泡的偏圆形大细胞同LH大量贮存有关。而液泡的消失以及大量小细胞和不规则形状细胞的出现同LH大量释放有密切联系。由此作者认为：LH细胞内液泡的动态变化以及由此产生的细胞大小、细胞形状和细胞结构的变化可以作为促性腺激素分泌的重要形态学观察指标。

关键词：内分泌生理垂体 LH lhrh 细胞形态

NOTE INDUCTION OF OVULATION IN FEMALE MUD SKIPPER (BOLEOPHTHALMUS PECTINIRESTRIS) BY HCG,ANALOGUE OF lhrh AND PIMOZIDE

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Chinese Journal of Oceanology and Limnology 1989年第3期7卷 283-287页

作者：洪万树王云龙 Department of Oceanology Xiamen University

The effects of two intraperitoneal injections of human chorionic gonadotropin (HCG) or [ D-ALA6, pro9- N- ethylamide]-luteinizing hormone releasing hormone analogue (lhrh-A), respectively, and the combined effect of lhrh-A with pimozide (PIM), a dopamine antagonist, on ovulation in female mud skipper were investigated. A high rate of ovulation (83.3%)was observed after injection with HCG(30 I. U./g). Injection of lhrh-A at a total dosage of 2μg/g was 100% effective in inducing ovulation. No ovulations occurred in the group receiving a low dosage of lhrh-A (0.02μg/g). Injection of lhrh-A(0.2μg/g)with PIM (10 or 20 μg/g)induced greater ovulatory response than injection with lhrh-A (0.2μg/g)alone. PIM at total dosages of 0.2μg/g or 2μg/g did not enhance the potency of lhrh-A to stimulate ovulation.

关键词： lhrh luteinizing gonadotropin alone chorionic inducing Injection antagonist receiving dopamine

Ability of luteinizing hormone releasing hormone-Pseudomonas aeruginosa exotoxin 40 binding to lhrh receptor on human liver cancer cells

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World Journal of Gastroenterology 2004年第19期10卷 2870-2873页

作者： Shou-LiangGong GangZhao Hong-GuangZhao Wen-TianLü Guang-WeiLiu PingZhu RadiobiologyResearchUnitofMinistryofPublicHealth SchoolofPublicHealthJilinUniversityChangchun130021JilinProvinceChina InstituteofMilitaryVeterinaryMedicine QuartermasterUniversityofChinesePLAChangchun130062.JilinPrnvinceChina

AIM: TO explore the ability of recombinant toxin luteinizinghormone releasing hormone-Pseudomonas aeruginosaexotoxin 40 (lhrh-PE40) and lhrh binding to lhrh receptor(lhrhR) on the membrane surface of human liver cancerHEPG ***: lhrh was labeled by using 12sI with enzymaticreaction. The affinity and receptor volume of LHP, H-PE40and lhrh binding to lhrhR on the membrane surface ofhuman liver cancer cells were measured with radioligandreceptor ***: The specific activity of lhrh labeled with 12sIwas 2.7x 104 kBq/l^L, and its radiochemical purity reachedto 99.2-99.7%. The binding of 12sI to lhrh was maximalfor 240 min in the warm cultivation, and this binding wasstabilized. The inhibiting rates of ~2SI-lhrh and lhrh onthe proliferation of human liver cancer HEPG cells werenot significantly different. On the basis of the saturationcurve of ~2SI-lhrh binding to the membrane lhrhR of HEPGcells, ~2SI-lhrh of lx10s cpm was selected for radioligandreceptor assay. The affinity constants (Kd) of lhrh-PE40and lhrh binding to the membrane lhrhR of HEPG cellswere 0.43~0.12 nmol/L and 4.86~ 1.47 nmol/L, respectively,and their receptor volumes were 0.37~0.15 l^mol/g and0.42=I=0.13 l^mol/g, respectively. The binding of lhrh-PE40to the membrane protein of normal liver cells was ***: The recombinant toxin lhrh-PE40 binding tothe membrane surface of lhrhR of human liver cancer HEPGcells was very strong, while the specific binding of it to normalliver cells was not observed. The results provide an importantexperimental basis for the clinical application of lhrh-PE.

关键词：黄体激素激素释放荷尔蒙-假单胞激素铜绿色外毒素40 lhrh 粘合物受体肝细胞癌肿瘤

lhrh-PE40对人结肠癌细胞增殖抑制和凋亡的影响

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吉林大学学报（医学版） 2006年第4期32卷 647-650页

作者：姜洋宋燕张颖超吴广谋张国利朱平吉林大学中日联谊医院普通外科吉林长春130033 吉林大学第二医院普通外科吉林长春130041 解放军军事医学科学院11所吉林长春130062

目的：探讨一种新型免疫毒素lhrh-PE40与人结肠癌细胞系Lovo表面lhrh受体结合的特性，观察其对细胞增殖产生的抑制作用及检测细胞凋亡。方法：用^125Ⅰ标记法检测lhrh-PE40与Lovo结合的特异性；MTT比色法检测lhrh-PE40对胃癌细胞的杀伤... 详细信息

目的：探讨一种新型免疫毒素lhrh-PE40与人结肠癌细胞系Lovo表面lhrh受体结合的特性，观察其对细胞增殖产生的抑制作用及检测细胞凋亡。方法：用^125Ⅰ标记法检测lhrh-PE40与Lovo结合的特异性；MTT比色法检测lhrh-PE40对胃癌细胞的杀伤活性，流式细胞仪检测细胞凋亡情况。结果：人结肠癌细胞系Lovo细胞表面可见配基一受体特异性结合，lhrh-PE40对Lovo细胞的半数致死量为0．24mg·L^-1，0．1～10．0mg·L^-1 lhrh-PE40作用于Lovo细胞，其凋亡明显（P〈0．01）。结论：结肠癌细胞Lovo细胞表面存在lhrh受体，lhrh-PE40对结肠癌细胞的增殖状态有明显的抑制作用及促凋亡作用。

关键词： lhrh-PE40 结肠肿瘤细胞凋亡受体 lhrh

介绍一种lhrh调节LH分泌的离体模型——分散垂体细胞灌流技术

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生理学报 1990年第1期42卷 89-96页

作者：屈智超喻晶华四川省计划生育科学研究所生理室世界卫生组织人类生殖研究合作中心

成年雄性 SD 大鼠断头后分离出垂体前叶(anterior pituitary,AP)。用胰蛋白酶消化和机械分散制备 AP 细胞(成活率大于95%)。分散的细胞悬液与生物凝胶混合后装上灌流柱,然后用 M199溶液连续灌流24h 以上。每间隔1~h 给予一次6min 的 LH... 详细信息

成年雄性 SD 大鼠断头后分离出垂体前叶(anterior pituitary,AP)。用胰蛋白酶消化和机械分散制备 AP 细胞(成活率大于95%)。分散的细胞悬液与生物凝胶混合后装上灌流柱,然后用 M199溶液连续灌流24h 以上。每间隔1~h 给予一次6min 的 lhrh 脉冲式刺激。细胞在此灌流过程中有一个稳定的基础 LH 分泌水平。lhrh 刺激能迅速引起 LH 分泌。对同一剂量 lhrh 的多次刺激可产生相同的 LH 脉冲。在一定的 lhrh 浓度范围内(1×10^(-10)_1×10^(-7)mol/L),LH 分泌与 lhrh 的剂量-效应曲线呈线性。实验结果表明,连续灌流分散的 AP 细胞的技术,优于单层细胞培养和组织块灌流等其他方法,是一种较为理想的研究lhrh 调节 LH 分泌机理的体外模型。

关键词：分散垂体前叶细胞灌流系统离体模型 lhrh LH分泌

lhrh类似物对体外培养的孕妇胎盘组织分泌hCG及孕酮的影响

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中国医药工业杂志 1990年第3期21卷 119-122页

作者：朱宝亭褚云鸿上海医科大学基础医学部药理学教研室上海200032

应用胎盘绒毛组织体外培养法和hCG、孕酮的放射免疫测定法,提示lhrh类似物对体外培养的人早孕(10～12周)和孕终末期(38～40周)绒毛组织分泌hCG均起兴奋作用。但对孕酮的分泌则影响不一;对早孕绒毛分泌孕酮有明显的抑制作用;而对孕终末... 详细信息

应用胎盘绒毛组织体外培养法和hCG、孕酮的放射免疫测定法,提示lhrh类似物对体外培养的人早孕(10～12周)和孕终末期(38～40周)绒毛组织分泌hCG均起兴奋作用。但对孕酮的分泌则影响不一;对早孕绒毛分泌孕酮有明显的抑制作用;而对孕终末期绒毛分泌孕酮却起兴奋作用。

关键词： lhrh hCG 孕酮胎盘组织药理

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lhrh兴奋试验在闭经处理上的应用

中华内分泌代谢杂志 1990年第1期6卷 23-25页

作者：张以文谷春霞徐苓张德永王惠兰北京协和医院妇产科

本文对18例正常妇女及50例血清促性腺激素正常或低下的闭经患者行lhrh兴奋试验(单次静脉注射法)。建立了早卵泡期妇女正常反应范围。78％闭经患者反应正常或高亢。本试验反应类型与预后及lhrh脉冲治疗效果之间有一定的相关关系。本文对L... 详细信息

本文对18例正常妇女及50例血清促性腺激素正常或低下的闭经患者行lhrh兴奋试验(单次静脉注射法)。建立了早卵泡期妇女正常反应范围。78％闭经患者反应正常或高亢。本试验反应类型与预后及lhrh脉冲治疗效果之间有一定的相关关系。本文对lhrh兴奋试验的临床价值及适应症进行了讨论。

关键词：闭经 lhrh 兴奋试验

Mechanisms of Prostate Atrophy after lhrh Antagonist Cetrorelix Injection: An Experimental Study in a Rat Model of Benign Prostatic Hyperplasia

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2012年第3期32卷 389-395页

作者：杨栋侯腾杨雄马彦汪隆旺李兵 Department of Urology Union Hospital Tongji Medical College Huazhong University of Science and Technology Department of Urology Shenzhen People's Hospital Second Clinical Medical College Jinan University

In the present study, we investigated the roles of TGF-β signaling pathway in a rat benign prostatic hyperplasia （BPH） model treated with cetrorelix. TGF-β1 and c-Myc expression were measured by qRT-PCR and Western blotting in the proximal and distal region of ventral prostatic lobes, respectively. We observed that treatment with cetrorelix led to a significant reduction of ventral prostate weight in a dose-dependent manner. In the proximal region, after cetrorelix treatment, the expression of TGF-β1 was dramatically increased （P〈0.05）, while the expression of c-Myc was significantly decreased （P〈0.05）. In comparison with the control group, the cetrorelix groups had more TUNEL-positive cells. Our findings strongly suggest that the TGF-β signaling pathway may be one of the major causes responsible for prostate volume reduction in BPH rats after cetrorelix treatment.

关键词： benign prostatic hyperplasia lhrh transforming growth factor-β